{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T19:04:50Z","timestamp":1789239890629,"version":"build-2803163510"},"reference-count":39,"publisher":"American Chemical Society (ACS)","issue":"28","license":[{"start":{"date-parts":[[2025,7,9]],"date-time":"2025-07-09T00:00:00Z","timestamp":1752019200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001823","name":"Ministerstvo ??kolstv??, Ml??de??e a T??lov??chovy","doi-asserted-by":"publisher","award":["8F20011"],"award-info":[{"award-number":["8F20011"]}],"id":[{"id":"10.13039\/501100001823","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100013281","name":"Joint Programming Initiative on Antimicrobial Resistance","doi-asserted-by":"publisher","award":["549009004"],"award-info":[{"award-number":["549009004"]}],"id":[{"id":"10.13039\/100013281","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007397","name":"Univerzita Karlova v Praze","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007397","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,7,22]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>According to the data from the World Health Organization, acute lower respiratory tract infections, such as pneumonia, are the leading causes of death in low- and middle-income countries, where the burden is highest. Adequate treatment of pneumonia requires accurate diagnosis; however, current diagnostic methods do not allow for rapid differentiation of viral and bacterial cause of pneumonia. Therefore, many patients are empirically treated, which leads to the inappropriate use of antibiotics and the acceleration of the emergence of antimicrobial resistance. We aimed to develop a fast and reliable detection method that is fit for purpose in low-resource settings to identify the most common pathogen causing pneumonia, namely Streptococcus pneumoniae. In this study, we developed, optimized, and validated a loop-mediated isothermal amplification (LAMP) method with simplified sample processing to detect S. pneumoniae in clinical swab samples. When tested on clinical samples, the simplified LAMP method demonstrated performance to that of the established PCR detection method. However, the LAMP approach does not require an expensive thermal cycler and allows for sensitive detection with a short turnaround time. Moreover, we engineered a simple, affordable device that, together with the method, can be implemented in low- and middle-income countries for point-of-care applications.<\/jats:p>","DOI":"10.1021\/acsomega.5c01541","type":"journal-article","created":{"date-parts":[[2025,7,9]],"date-time":"2025-07-09T19:06:09Z","timestamp":1752087969000},"page":"30250-30258","source":"Crossref","is-referenced-by-count":1,"title":["Simplified Loop-Mediated\nIsothermal Amplification-Based\nMethod for Point-of-Care Detection of\n                    <i>Streptococcus\npneumoniae<\/i>\n                    in Low-Resource Settings"],"prefix":"10.1021","volume":"10","author":[{"given":"Petr","family":"Jer\u030ca\u0301bek","sequence":"first","affiliation":[{"name":"Charles University , , ,","place":["Prague, The Czech\nRepublic, 128 00"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0865-8785","authenticated-orcid":true,"given":"Marke\u0301ta","family":"Marti\u0301nkova\u0301","sequence":"additional","affiliation":[{"name":"Charles University , , ,","place":["Prague, The Czech\nRepublic, 128 00"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matu\u0301s\u030c","family":"Fric\u030cek","sequence":"additional","affiliation":[{"name":"Charles University , , ,","place":["Prague, The Czech\nRepublic, 128 00"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christa E.","family":"van der Gaast \u2212 de Jongh","sequence":"additional","affiliation":[{"name":"Radboud University Medical Center , , ,","place":["Nijmegen, The Netherlands, 6525 GA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denis R.","family":"Katundu","sequence":"additional","affiliation":[{"name":"Kilimanjaro Christian Medical University College , ,","place":["Kilimanjaro, Tanzania"]},{"name":"Radboud University Medical Center , , ,","place":["Nijmegen, The Netherlands, 6525 GA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niels","family":"van Heerbeek","sequence":"additional","affiliation":[{"name":"Kilimanjaro Christian Medical University College , ,","place":["Kilimanjaro, Tanzania"]},{"name":"Radboud University Medical Center , , ,","place":["Nijmegen, The Netherlands, 6525 GA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corne\u0301 H.","family":"van den Kieboom","sequence":"additional","affiliation":[{"name":"Xheal B.V. , Reinier Postlaan 2 , ,","place":["Nijmegen, The Netherlands, 6525 GC"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marien I.","family":"de Jonge","sequence":"additional","affiliation":[{"name":"Radboud University Medical Center , , ,","place":["Nijmegen, The Netherlands, 6525 GA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3321-4526","authenticated-orcid":true,"given":"Va\u0301clav","family":"Marti\u0301nek","sequence":"additional","affiliation":[{"name":"Charles University , , ,","place":["Prague, The Czech\nRepublic, 128 00"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2025,7,9]]},"reference":[{"issue":"10325","key":"2026081812212568100_cit1","doi-asserted-by":"publisher","first-page":"629","DOI":"10.1016\/S0140-6736(21)02724-0","article-title":"Global Burden of Bacterial\nAntimicrobial Resistance in 2019: A Systematic Analysis","volume":"399","author":"Murray","year":"2022","journal-title":"Lancet"},{"key":"2026081812212568100_cit2","doi-asserted-by":"publisher","DOI":"10.3389\/fpubh.2022.1028525","article-title":"Global Burden\nof Lower Respiratory\nInfections during the Last Three Decades","volume":"10","author":"Safiri","year":"2023","journal-title":"Front\nPublic Health"},{"key":"2026081812212568100_cit3","unstructured":"The top 10 causes\nof death. https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/the-top-10-causes-of-death (accessed 2025\u201303\u201312)."},{"issue":"1","key":"2026081812212568100_cit4","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1186\/s12879-021-06996-7","article-title":"Nasopharyngeal Colonisation\nDynamics of Bacterial Pathogens in Patients with Fever in Rural Burkina\nFaso: An Observational Study","volume":"22","author":"Martens","year":"2022","journal-title":"BMC Infect Dis"},{"issue":"7","key":"2026081812212568100_cit5","doi-asserted-by":"publisher","first-page":"e744","DOI":"10.1016\/S2214-109X(18)30247-X","article-title":"Burden\nof Streptococcus Pneumoniae and Haemophilus Influenzae Type b Disease\nin Children in the Era of Conjugate Vaccines: Global, Regional, and\nNational Estimates for 2000\u201315","volume":"6","author":"Wahl","year":"2018","journal-title":"Lancet\nGlob Health"},{"issue":"11","key":"2026081812212568100_cit6","doi-asserted-by":"publisher","first-page":"e774","DOI":"10.1016\/S2589-7500(23)00149-8","article-title":"A Multi-Platform Approach to Identify\na Blood-Based Host Protein Signature for Distinguishing between Bacterial\nand Viral Infections in Febrile Children (PERFORM): A Multi-Cohort\nMachine Learning Study","volume":"5","author":"Jackson","year":"2023","journal-title":"Lancet Digital Health"},{"issue":"6","key":"2026081812212568100_cit7","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1177\/2042098614554919","article-title":"Antimicrobial Resistance:\nRisk Associated with Antibiotic\nOveruse and Initiatives to Reduce the Problem","volume":"5","author":"Llor","year":"2014","journal-title":"Ther Adv. 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Chem."},{"issue":"32","key":"2026081812212568100_cit30","doi-asserted-by":"publisher","first-page":"16722","DOI":"10.1021\/acs.langmuir.4c00793","article-title":"In Situ Colorimetric\nLAMP Based on One-Step Modified Filter Paper to Screen Human Papillomavirus\n(HPV)\u00ad16\/18 from Clinical Samples","volume":"40","author":"Jiao","year":"2024","journal-title":"Langmuir"},{"issue":"37","key":"2026081812212568100_cit31","doi-asserted-by":"publisher","first-page":"38855","DOI":"10.1021\/acsomega.4c05025","article-title":"Comparison and Optimization of Simple\nDNA Extraction Methods for LAMP-Based Point-of-Care Applications Employing\nSubmillimeter Skin Biopsies","volume":"9","author":"Boza","year":"2024","journal-title":"ACS Omega"},{"key":"2026081812212568100_cit32","doi-asserted-by":"publisher","first-page":"2668","DOI":"10.1021\/acsinfecdis.4c00013","article-title":"Naked-Eye\nLAMP Assay of M. Tuberculosis in Sputum by In Situ Au Nanoprobe Identification:\nFor the In Vitro Diagnostics of Tuberculosis","volume":"10","author":"Zhang","year":"2024","journal-title":"ACS Infect. Dis."},{"key":"2026081812212568100_cit33","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1016\/B978-0-12-416999-9.00007-1","article-title":"Chapter 7 - DNA\nExtraction: Finding the Most Suitable Method","volume-title":"Molecular Microbial Diagnostic Methods","author":"Barbosa","year":"2016"},{"issue":"11","key":"2026081812212568100_cit34","doi-asserted-by":"publisher","first-page":"3007","DOI":"10.1002\/bit.28198","article-title":"New Horizons\nin Developing Cell Lysis Methods: A Review","volume":"119","author":"Danaeifar","year":"2022","journal-title":"Biotechnol. 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