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Unfortunately, it was determined that the immobilized enzyme cannot be recycled effectively. The optimized acetylation method was screened with different thioglycosides and with fully unprotected saccharides. New methods for several new partially protected saccharides were found, while the synthesis of some known saccharides, e.g., the third and the sixth position-protected d-glucose or the fourth position-protected l-rhamnose, was improved. Furthermore, an enzymatic acetal formation between the fourth and the sixth positions was discovered. The main limitation for acetylation reactions with CAL-B has been determined to be the substrate solubility.<\/jats:p>","DOI":"10.1021\/acsomega.5c02467","type":"journal-article","created":{"date-parts":[[2025,5,7]],"date-time":"2025-05-07T11:53:06Z","timestamp":1746618786000},"page":"20047-20053","source":"Crossref","is-referenced-by-count":1,"title":["Selective Acetylation\nof Unprotected Thioglycosides\nand Fully Unprotected Monosaccharides with\n                    <i>Candida\nantarctica<\/i>\n                    Lipase\u2011B"],"prefix":"10.1021","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3860-0194","authenticated-orcid":true,"given":"Kaarel\nErik","family":"Hunt","sequence":"first","affiliation":[{"name":"Tallinn University of Technology , , Akadeemia tee 15 , ,","place":["Tallinn, Estonia, 12618"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5014-6839","authenticated-orcid":true,"given":"Annette","family":"Miller","sequence":"additional","affiliation":[{"name":"Tallinn University of Technology , , Akadeemia tee 15 , ,","place":["Tallinn, Estonia, 12618"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsiana","family":"Jarg","sequence":"additional","affiliation":[{"name":"Tallinn University of Technology , , Akadeemia tee 15 , ,","place":["Tallinn, Estonia, 12618"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2503-7788","authenticated-orcid":true,"given":"Kadri","family":"Kriis","sequence":"additional","affiliation":[{"name":"Tallinn University of Technology , , Akadeemia tee 15 , ,","place":["Tallinn, Estonia, 12618"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5339-9682","authenticated-orcid":true,"given":"To\u0303nis","family":"Kanger","sequence":"additional","affiliation":[{"name":"Tallinn University of Technology , , Akadeemia tee 15 , ,","place":["Tallinn, Estonia, 12618"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2025,5,7]]},"reference":[{"issue":"12","key":"2026081811201011100_cit1","doi-asserted-by":"publisher","first-page":"2371","DOI":"10.1021\/acs.orglett.2c00596","article-title":"Synthesis of Oligosaccharides Resembling\nthe Streptococcus Suis Serotype 18 Capsular Polysaccharide\nas a Basis for Glycoconjugate Vaccine Development","volume":"24","author":"Singh","year":"2022","journal-title":"Org. 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