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This study aimed to assess the antifungal efficacy and survival benefits of RN104 incorporated into a self-emulsifying drug delivery system (SEDDS-RN104) compared to free RN104 and fluconazole (FCZ) in a murine model of Cryptococcus neoformans (H99) infection. The results demonstrated that SEDDS-RN104 significantly enhanced survival rates compared to free RN104 and FCZ, reducing the event rate (death) by 78% compared to FCZ. The mean survival time was 25 days in the SEDDS-RN104 group, compared to 14 days for free RN104 and 19 days for FCZ. Additionally, the fungal burden in the lungs was markedly reduced in the SEDDS-RN104 group, as confirmed by histopathological analysis. These findings suggest that SEDDS-RN104 effectively addresses the pharmacokinetic limitations of RN104, enhancing its antifungal efficacy and positioning it as a promising therapeutic alternative for cryptococcal infections.<\/jats:p>","DOI":"10.1021\/acsomega.5c02705","type":"journal-article","created":{"date-parts":[[2025,6,21]],"date-time":"2025-06-21T13:48:58Z","timestamp":1750513738000},"page":"27301-27307","source":"Crossref","is-referenced-by-count":1,"title":["Antifungal Efficacy\nof a Thiazolyl Hydrazone Derivative\nIncorporated into a Self-Emulsifying Drug Delivery System in a Murine\nModel of Cryptococcosis"],"prefix":"10.1021","volume":"10","author":[{"given":"Iara R.","family":"Silva","sequence":"first","affiliation":[{"name":"Universidade Federal de Minas Gerais , , Av. 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