{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T02:41:30Z","timestamp":1791340890941,"version":"4.3.1"},"reference-count":18,"publisher":"American Chemical Society (ACS)","issue":"28","license":[{"start":{"date-parts":[[2025,7,10]],"date-time":"2025-07-10T00:00:00Z","timestamp":1752105600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo","doi-asserted-by":"publisher","award":["2018\/18954-4"],"award-info":[{"award-number":["2018\/18954-4"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda??o de Amparo ? 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This study aimed to identify novel anti-Toxoplasma gondii compounds through the phenotypic screening of the Medicines for Malaria Venture (MMV) Global Health Priority Box (GHPB), a curated library of 240 chemically diverse molecules. Parasite viability and host cell cytotoxicity assays identified six lead compounds with the selectivity index (SI) exceeding 100, with MMV689404 (Triflumuron), MMV1794214 (Vaniliprole), and MMV1794211 showing SI &amp;gt;103, &amp;gt; 756, and &amp;gt;1000, respectively. In silico ADMET profiling revealed favorable pharmacokinetic and toxicity parameters for most hits, although Vaniliprole showed suboptimal gastrointestinal absorption and potential tumorigenicity. Comparative analysis with the existing literature confirmed previously reported antiparasitic activity for several compounds, reinforcing their relevance for repurposing. These findings highlight the potential of GHPB as a source of candidate molecules for further preclinical evaluation against toxoplasmosis.<\/jats:p>","DOI":"10.1021\/acsomega.5c05130","type":"journal-article","created":{"date-parts":[[2025,7,10]],"date-time":"2025-07-10T19:56:42Z","timestamp":1752177402000},"page":"31147-31152","source":"Crossref","is-referenced-by-count":5,"title":["Phenotypic Screening\nof the MMV Global Health Priority\nBox Identifies Selective Compounds with Anti-\n                    <i>Toxoplasma\ngondii<\/i>\n                    Activity"],"prefix":"10.1021","volume":"10","author":[{"given":"Gabriel\nCandido","family":"Moura","sequence":"first","affiliation":[{"name":"Faculdade de Medicina de Jundiai\u0301 , , ,","place":["Jundiai\u0301, Sa\u0303o Paulo, Brazil, 13202-550"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4418-0838","authenticated-orcid":true,"given":"Juliana Quero","family":"Reima\u0303o","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina de Jundiai\u0301 , , ,","place":["Jundiai\u0301, Sa\u0303o Paulo, Brazil, 13202-550"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2025,7,10]]},"reference":[{"key":"2026081813070850200_cit1","doi-asserted-by":"publisher","first-page":"3059","DOI":"10.1007\/s00436-018-6040-2","article-title":"Toxoplasma gondii infection and behavioral\noutcomes\nin humans: a systematic review","volume":"117","author":"Martinez","year":"2018","journal-title":"Parasitol. Res."},{"key":"2026081813070850200_cit2","doi-asserted-by":"publisher","first-page":"805","DOI":"10.3109\/00365548.2012.693197","article-title":"A review on human toxoplasmosis","volume":"44","author":"Saadatnia","year":"2012","journal-title":"Scand. J. Infect. Dis."},{"key":"2026081813070850200_cit3","doi-asserted-by":"publisher","DOI":"10.1016\/j.fawpar.2019.e00036","article-title":"Treatment\nof toxoplasmosis: Current options and future perspectives","volume":"15","author":"Konstantinovic","year":"2019","journal-title":"Food Waterborne Parasitol."},{"key":"2026081813070850200_cit4","doi-asserted-by":"publisher","first-page":"4030","DOI":"10.1021\/acsinfecdis.4c00700","article-title":"Global\nHealth Priority Box\u2013Proactive Pandemic\nPreparedness","volume":"10","author":"Adam","year":"2024","journal-title":"ACS Infect. Dis."},{"key":"2026081813070850200_cit5","doi-asserted-by":"publisher","DOI":"10.12688\/verixiv.197.1","article-title":"Discovery of a Picomolar Antiplasmodial\nPyrazole\nDerivative from a Broad Antiparasitic Screening of Compounds from\nthe MMV Open Access Global Health Priority Box","volume":"1","author":"Tali","year":"2024","journal-title":"VeriXiv"},{"key":"2026081813070850200_cit7","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2024.106828","article-title":"Screening of the global\nhealth priority Box reveals\npotential new disinfectants against the emerging multidrug-resistant\npathogen Candida auris","volume":"194","author":"Retore","year":"2024","journal-title":"Microb. Pathog."},{"key":"2026081813070850200_cit6","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25116227","article-title":"Novel\ncompound MMV1804559 from the\nglobal health priority box exhibits in vitro and in vivo activity\nagainst Madurella mycetomatis","volume":"25","author":"Ma","year":"2024","journal-title":"Int. J. Mol. Sci."},{"key":"2026081813070850200_cit13","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-024-05155-y","article-title":"Screening the Global Health Priority Box against Plasmodium berghei liver stage parasites using an\ninexpensive luciferase detection protocol","volume":"23","author":"Nguyen","year":"2024","journal-title":"Malar.\nJ."},{"key":"2026081813070850200_cit8","doi-asserted-by":"publisher","DOI":"10.3390\/ph17050554","article-title":"Global Health Priority Box: Discovering\nflucofuron\nas a promising antikinetoplastid compound","volume":"17","author":"Bethencourt-Estrella","year":"2024","journal-title":"Pharmaceuticals"},{"key":"2026081813070850200_cit9","doi-asserted-by":"publisher","first-page":"2063","DOI":"10.1021\/acsinfecdis.4c00062","article-title":"Flucofuron\nas a Promising Therapeutic Agent against\nBrain-Eating Amoeba","volume":"10","author":"Chao-Pellicer","year":"2024","journal-title":"ACS Infect. 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Agents Chemother."},{"key":"2026081813070850200_cit14","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.22504","article-title":"Triflumuron\ninduces cytotoxic effects on hepatic and renal human cell lines","volume":"34","author":"Timoumi","year":"2020","journal-title":"J. Biochem. Mol. Toxicol."},{"key":"2026081813070850200_cit15","doi-asserted-by":"publisher","first-page":"3723","DOI":"10.1007\/s11356-018-3908-8","article-title":"Acute triflumuron exposure induces\noxidative stress responses in liver and kidney of Balb\/C mice","volume":"26","author":"Timoumi","year":"2019","journal-title":"Environ. Sci. Pollut. Res."},{"key":"2026081813070850200_cit16","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0288335","article-title":"Repurposing\nthe Medicines for Malaria Venture\u2019s COVID Box to discover potent\ninhibitors of Toxoplasma gondii, and in vivo efficacy evaluation of\nalmitrine bismesylate (MMV1804175) in chronically infected mice","volume":"18","author":"Dos\nSantos","year":"2023","journal-title":"PLoS One"},{"key":"2026081813070850200_cit17","doi-asserted-by":"publisher","DOI":"10.1038\/srep42717","article-title":"SwissADME: a free web\ntool to evaluate\npharmacokinetics, drug-likeness and medicinal chemistry friendliness\nof small molecules","volume":"7","author":"Daina","year":"2017","journal-title":"Sci. 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