{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T19:37:18Z","timestamp":1791747438457,"version":"4.3.4"},"reference-count":112,"publisher":"American Chemical Society (ACS)","issue":"33","license":[{"start":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T00:00:00Z","timestamp":1786060800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100032205","name":"Association of African Universities","doi-asserted-by":"publisher","award":["Ref. PC\/6"],"award-info":[{"award-number":["Ref. 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(Malvaceae) plant were subjected to maceration in methanol, fractionation in mixed solvent systems, and solvent-gradient column chromatography to furnish 20-hydroxyecdysone 8 and ajugasterone C 24 as pale-yellow and yellow powders in 0.62% and 0.25% yields, respectively. The crude methanol extract and fractions were assayed against Escherichia coli ATCC 25922, enterohemorrhagic E. coli (EHEC) O157:H7, Shigella flexneri ATCC 12022, Salmonella Typhi ATCC 13311, and coded clinical diarrheagenic E. coli isolates: DEC ANI and DEC 134, to assess in vitro antidiarrheal activity. The extract exhibited an MIC of 17.5 mg\/mL against DEC ANI, whereas fraction F11 recorded an MIC of 8.75 mg\/mL against EHEC and S. flexneri. The docking studies of compounds 8 and 24 with proteins of S. Typhi (PDB id: 4KRA) and EHEC (PDB id: 1AB4) revealed higher binding affinities of \u221212.12 kcal\/mol and \u221212.70 kcal\/mol, respectively, against the S. Typhi protein, compared to \u221211.09 kcal\/mol of ciprofloxacin (reference drug), indicating potential antidiarrheal properties.<\/jats:p>","DOI":"10.1021\/acsomega.5c11934","type":"journal-article","created":{"date-parts":[[2026,8,8]],"date-time":"2026-08-08T01:01:43Z","timestamp":1786150903000},"page":"48869-48882","source":"Crossref","is-referenced-by-count":1,"title":["Isolation of Phytoecdysteroids:\nAjugasterone C and\n20-Hydroxyecdysone, from\n                    <i>Sida alba<\/i>\n                    Linn."],"prefix":"10.1021","volume":"11","author":[{"given":"Chijioke N.","family":"Ezennaka","sequence":"first","affiliation":[{"name":"University of Lagos , , Akoka-Yaba , ,","place":["Lagos, Nigeria, 100213"]},{"name":"University of Mississippi , , , ,","place":["University, Mississippi, United States, 38677"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Radhakrishnan","family":"Srivedavyasasri","sequence":"additional","affiliation":[{"name":"University of Mississippi , , , ,","place":["University, Mississippi, United States, 38677"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6315-4969","authenticated-orcid":true,"given":"Josephat U.","family":"Izunobi","sequence":"additional","affiliation":[{"name":"University of Lagos , , Akoka-Yaba , ,","place":["Lagos, Nigeria, 100213"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3202-3536","authenticated-orcid":true,"given":"Justice J.","family":"Mallen","sequence":"additional","affiliation":[{"name":"University of Wisconsin , , , ,","place":["Milwaukee, Wisconsin, United States, 53211"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaret O.","family":"Sofidiya","sequence":"additional","affiliation":[{"name":"University of Lagos , , Idi-Araba , ,","place":["Lagos, Nigeria, 101014"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olayinka T.","family":"Asekun","sequence":"additional","affiliation":[{"name":"University of Lagos , , Akoka-Yaba , ,","place":["Lagos, Nigeria, 100213"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nwamaka H.","family":"Igbokwe","sequence":"additional","affiliation":[{"name":"University of Lagos , , Idi-Araba , ,","place":["Lagos, Nigeria, 101014"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-1895-9701","authenticated-orcid":true,"given":"Stephen T.","family":"Nkwocha","sequence":"additional","affiliation":[{"name":"University of Wisconsin , , , ,","place":["Milwaukee, Wisconsin, United States, 53211"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3807-1299","authenticated-orcid":true,"given":"Samir A.","family":"Ross","sequence":"additional","affiliation":[{"name":"University of Mississippi , , , ,","place":["University, Mississippi, United States, 38677"]},{"name":"University of Mississippi , , , ,","place":["University, Mississippi, United States, 38677"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,7]]},"reference":[{"key":"2026083119112988300_cit1","unstructured":"World Health Organization (WHO).\n          . 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Mol. Biomed. Rep."},{"issue":"9","key":"2026083119112988300_cit56","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0275229","article-title":"Molecular identification\nof diarrheagenic Escherichia coli pathotypes\nand their antibiotic resistance patterns among diarrheic children\nand in contact calves in Bahir Dar city, Northwest Ethiopia","volume":"17","author":"Belete","year":"2022","journal-title":"PLoS One"},{"key":"2026083119112988300_cit57","unstructured":"Thai, T.; Salisbury, B. H.; Zito, P. M.\n          Ciprofloxacin.  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