{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T07:27:57Z","timestamp":1787988477370,"version":"build-2784847793"},"reference-count":43,"publisher":"American Chemical Society (ACS)","issue":"9","license":[{"start":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T00:00:00Z","timestamp":1771891200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["APQ-01996-23"],"award-info":[{"award-number":["APQ-01996-23"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["APQ-04955-23"],"award-info":[{"award-number":["APQ-04955-23"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["RED-00120-23"],"award-info":[{"award-number":["RED-00120-23"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["313367\/2021-3"],"award-info":[{"award-number":["313367\/2021-3"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["315640\/2021-9"],"award-info":[{"award-number":["315640\/2021-9"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["405620\/2021-7"],"award-info":[{"award-number":["405620\/2021-7"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100018992","name":"Fonds der Chemischen Industrie","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100018992","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2023\/07323-1"],"award-info":[{"award-number":["2023\/07323-1"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,3,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>This work addresses the detection and quantification of paracetamol (PAR) in human serum using a point-of-care approach, an essential assessment for intoxication cases in clinical analysis. Electrochemical and colorimetric methods have been widely reported for simple and fast determination of PAR in biological samples. However, most of these previous portable methods have neglected some aspects for a real-world clinical application of PAR intoxication, such as its toxic concentration range and a proper interference study in biological matrices. To fill this gap, we introduce a novel integrated sensing approach, combining colorimetric and electrochemical techniques for determining PAR. In contrast to other sensors, this work employs a simple screen-printed graphite electrode (SPE-Gr) combined with a colorimetric reagent (CR) for the rapid and selective detection of PAR in human serum. Analysis occurs via dual confirmation: after reaction between PAR and CR, first a color change can indicate intoxication levels, and the generated product is subsequently accurately quantified by square-wave adsorptive stripping voltammetry using a SPE-Gr. A wide linear range (4.5 to 1000 mg L\u20131) for determining PAR was obtained, with a limit of detection (1.2 mg L\u20131), covering its therapeutic and toxic concentrations for assessment of intoxication cases. High reproducibility was demonstrated by low relative standard deviations (RSDs &amp;lt; 2.0%) and consistent responses obtained with different disposable SPEs-Gr (n = 3). Analysis in real human serum with a comprehensive interference study showed a high selectivity and reliability of the proposed method for PAR detection in biological samples. Therefore, our combined electrochemical-colorimetric method provides a suitable point-of-care sensor for PAR determination, enabling a prompt and straightforward diagnosis to reduce mortality in intoxication cases.<\/jats:p>","DOI":"10.1021\/acsomega.5c12147","type":"journal-article","created":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T13:37:25Z","timestamp":1771940245000},"page":"15094-15102","source":"Crossref","is-referenced-by-count":3,"title":["Rapid Point-of-Care Assessment of Paracetamol Intoxication\nvia an Integrated Electrochemical\u2013Colorimetric Method"],"prefix":"10.1021","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-7072-5208","authenticated-orcid":true,"given":"Anne A.","family":"Macedo","sequence":"first","affiliation":[{"name":"Universidade Federal Dos Vales Do Jequitinhonha e Mucuri (UFVJM) , , , ,","place":["Diamantina, Minas Gerais, Brazil, 39100000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8932-9739","authenticated-orcid":true,"given":"Dilton M.","family":"Pimentel","sequence":"additional","affiliation":[{"name":"PRPPG-UFVJM , , , ,","place":["Diamantina, Minas Gerais, Brazil, 39100000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karla A. O.","family":"Souza","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Campinas (UNICAMP) , , , ,","place":["Campinas, Sa\u0303o Paulo, Brazil, 13083-859"]},{"name":"UNICAMP , , , ,","place":["Campinas, Sa\u0303o Paulo, Brazil, 13083-859"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9607-3391","authenticated-orcid":true,"given":"Jose\u0301 L.","family":"Costa","sequence":"additional","affiliation":[{"name":"UNICAMP , , , ,","place":["Campinas, Sa\u0303o Paulo, Brazil, 13083-859"]},{"name":"UNICAMP , , , ,","place":["Campinas, Sa\u0303o Paulo, Brazil, 13083-859"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cla\u0301udia\nM.","family":"Rocha","sequence":"additional","affiliation":[{"name":"Universidade Federal de Minas Gerais (UFMG) , , , ,","place":["Belo\nHorizonte, Minas Gerais, Brazil, 31270-901"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cle\u0301sia C.","family":"Nascentes","sequence":"additional","affiliation":[{"name":"Universidade Federal de Minas Gerais (UFMG) , , , ,","place":["Belo\nHorizonte, Minas Gerais, Brazil, 31270-901"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2344-5590","authenticated-orcid":true,"given":"A\u0302ngelo","family":"de Fa\u0301tima","sequence":"additional","affiliation":[{"name":"Universidade Federal de Minas Gerais (UFMG) , , , ,","place":["Belo\nHorizonte, Minas Gerais, Brazil, 31270-901"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1705-1974","authenticated-orcid":true,"given":"Luciano C.","family":"Arantes","sequence":"additional","affiliation":[{"name":"UFMG , , , ,","place":["Belo Horizonte, Minas Gerais, Brazil, 31270-901"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fateme","family":"Ebrahimi","sequence":"additional","affiliation":[{"name":"University of Stuttgart , , ,","place":["Stuttgart, Germany, 70569"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9691-1903","authenticated-orcid":true,"given":"Bertold","family":"Rasche","sequence":"additional","affiliation":[{"name":"University of Stuttgart , , ,","place":["Stuttgart, Germany, 70569"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6585-9930","authenticated-orcid":true,"given":"Wallans T. P.","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Universidade Federal Dos Vales Do Jequitinhonha e Mucuri (UFVJM) , , , ,","place":["Diamantina, Minas Gerais, Brazil, 39100000"]},{"name":"University of Stuttgart , , ,","place":["Stuttgart, Germany, 70569"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,2,24]]},"reference":[{"key":"2026081508103442200_cit1","doi-asserted-by":"publisher","first-page":"1081","DOI":"10.2147\/JPR.S393809","article-title":"An updated\nreview on the metabolite AM404-mediated central mechanism of action\nof paracetamol (acetaminophen): experimental evidence and potential\nclinical impact","volume":"16","author":"Mallet","year":"2023","journal-title":"J. Pain Res."},{"key":"2026081508103442200_cit2","volume-title":"WHO Model List of Essential Medicines-22nd\nList","author":"World Health Organization","year":"2021"},{"key":"2026081508103442200_cit3","doi-asserted-by":"publisher","first-page":"735","DOI":"10.1001\/jama.2023.1080","article-title":"Association of FDA mandate limiting acetaminophen\n(paracetamol) in prescription combination opioid products and subsequent\nhospitalizations and acute liver failure","volume":"329","author":"Orandi","year":"2023","journal-title":"JAMA"},{"key":"2026081508103442200_cit4","doi-asserted-by":"publisher","first-page":"138","DOI":"10.1016\/j.jpha.2020.11.003","article-title":"Chemically modified\ncarbon-based electrodes for the determination of paracetamol in drugs\nand biological samples","volume":"11","author":"Boumya","year":"2021","journal-title":"J. Pharm. Anal."},{"key":"2026081508103442200_cit5","doi-asserted-by":"publisher","first-page":"297","DOI":"10.1080\/17425255.2023.2223959","article-title":"Paracetamol (acetaminophen)\noverdose and hepatotoxicity: mechanism, treatment, prevention measures,\nand estimates of burden of disease","volume":"19","author":"Chidiac","year":"2023","journal-title":"Expert Opin.\nDrug Metab. Toxicol."},{"key":"2026081508103442200_cit6","volume-title":"Acetaminophen Toxicity","author":"Agrawal","year":"2017"},{"key":"2026081508103442200_cit7","doi-asserted-by":"publisher","first-page":"699","DOI":"10.1016\/j.jad.2020.07.091","article-title":"Paracetamol self-poisoning:\nepidemiological study of\ntrends and patient characteristics from the multicentre study of self-harm\nin England","volume":"276","author":"Casey","year":"2020","journal-title":"J. Affective Disord."},{"key":"2026081508103442200_cit8","doi-asserted-by":"publisher","first-page":"692","DOI":"10.1080\/01480545.2022.2083149","article-title":"Decision tree\nalgorithm can determine the outcome of repeated supratherapeutic ingestion\nexposure to acetaminophen: review of 4500 National Poison Data System\ncases","volume":"46","author":"Mehrpour","year":"2023","journal-title":"Drug Chem. Toxicol."},{"key":"2026081508103442200_cit9","doi-asserted-by":"publisher","first-page":"871","DOI":"10.1542\/peds.55.6.871","article-title":"Acetaminophen poisoning and toxicity","volume":"55","author":"Rumack","year":"1975","journal-title":"Pediatrics"},{"key":"2026081508103442200_cit10","doi-asserted-by":"publisher","first-page":"175","DOI":"10.5694\/mja2.50428","article-title":"Updated guidelines for the management\nof paracetamol poisoning in Australia and New Zealand","volume":"212","author":"Chiew","year":"2020","journal-title":"Med. J. Aust."},{"key":"2026081508103442200_cit11","doi-asserted-by":"publisher","first-page":"343243","DOI":"10.1016\/j.aca.2024.343243","article-title":"Electrochemical\nmethods for the determination\nof acetaminophen in biological matrices: a critical review in the\nclinical field","volume":"1333","author":"Melo","year":"2025","journal-title":"Anal. Chim. Acta"},{"key":"2026081508103442200_cit12","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1016\/0163-7258(93)90023-7","article-title":"Paracetamol\n(acetaminophen) poisoning","volume":"60","author":"Thomas","year":"1993","journal-title":"Pharmacol. 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Innov"},{"key":"2026081508103442200_cit17","doi-asserted-by":"publisher","first-page":"101408","DOI":"10.1016\/j.coelec.2023.101408","article-title":"Recent advances\nin the use of screen-printed electrodes in drug analysis: a review","volume":"42","author":"Keli\u0301s\u030ckova\u0301","year":"2023","journal-title":"Curr. Opin. Electrochem."},{"key":"2026081508103442200_cit18","doi-asserted-by":"publisher","DOI":"10.1002\/elan.202400227","article-title":"Assessing the\nelectrochemical performance of a novel\nlow-cost screen-printed electrode based on Apis mellifera beeswax modified with Vulcan@Chitosan used for acetaminophen detection","volume":"36","author":"Gallina","year":"2024","journal-title":"Electroanalysis"},{"key":"2026081508103442200_cit19","doi-asserted-by":"publisher","DOI":"10.1002\/elan.202200484","article-title":"Simultaneous voltammetric determination\nof acetylsalicylic acid, caffeine, and paracetamol in pharmaceutical\nformulations using screen-printed carbon electrodes","volume":"35","author":"Corte\u0301s","year":"2023","journal-title":"Electroanalysis"},{"key":"2026081508103442200_cit20","doi-asserted-by":"publisher","DOI":"10.1002\/slct.202303369","article-title":"Screen-printed\ncarbon electrodes modified with poly (amino acids) for the simultaneous\ndetection of vitamin C and paracetamol","volume":"9","author":"Long","year":"2024","journal-title":"ChemistrySelect"},{"key":"2026081508103442200_cit21","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1016\/j.snb.2019.01.047","article-title":"A simple method to detect the stimulant\nmodafinil in authentic saliva using a carbon-nanotube screen-printed\nelectrode with adsorptive stripping voltammetry","volume":"285","author":"dos\nSantos","year":"2019","journal-title":"Sens. Actuators, B Chem."},{"key":"2026081508103442200_cit22","doi-asserted-by":"publisher","first-page":"134183","DOI":"10.1016\/j.snb.2023.134183","article-title":"Electrochemistry\nof the synthetic\ntryptamine 5-MeO-MiPT at glassy carbon and screen-printed electrodes:\na rapid and simple screening method for application in forensic analysis","volume":"393","author":"Melo","year":"2023","journal-title":"Sens. Actuators, B Chem."},{"key":"2026081508103442200_cit23","doi-asserted-by":"publisher","first-page":"4699","DOI":"10.1021\/acsanm.1c00310","article-title":"Simultaneous\nfemtomolar detection\nof paracetamol, diclofenac, and orphenadrine using a carbon nanotube\/zinc\noxide nanoparticle-based electrochemical sensor","volume":"4","author":"Kokab","year":"2021","journal-title":"ACS Appl. Nano Mater."},{"key":"2026081508103442200_cit24","doi-asserted-by":"publisher","first-page":"2351","DOI":"10.1002\/elan.202100325","article-title":"A novel electrochemical\nglassy carbon electrode modified with carbon black and glyceline deep\neutectic solvent within a crosslinked chitosan film for simultaneous\ndetermination of acetaminophen and diclofenac","volume":"33","author":"Piton","year":"2021","journal-title":"Electroanalysis"},{"key":"2026081508103442200_cit25","doi-asserted-by":"publisher","first-page":"3581","DOI":"10.3390\/ma14133581","article-title":"Electrochemically activated\nscreen-printed carbon sensor modified with anionic surfactant for\nsimultaneous determination of paracetamol, diclofenac, and tramadol","volume":"14","author":"Kozak","year":"2021","journal-title":"Materials"},{"key":"2026081508103442200_cit26","doi-asserted-by":"publisher","first-page":"9910","DOI":"10.1038\/srep09910","article-title":"Office paper\nplatform for bioelectrochromic detection\nof electrochemically active bacteria using tungsten trioxide nanoprobes","volume":"5","author":"Marques","year":"2015","journal-title":"Sci. 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Educ."},{"key":"2026081508103442200_cit30","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1021\/jo01193a004","article-title":"The condensation\nof aminoantipyrine. II. A new color test for phenolic compounds","volume":"08","author":"Emerson","year":"1943","journal-title":"J. Org. Chem."},{"key":"2026081508103442200_cit31","doi-asserted-by":"publisher","first-page":"110717","DOI":"10.1016\/j.microc.2024.110717","article-title":"Enhanced detection of lysergic acid\ndiethylamide using Ehrlich reagent and screen-printed electrodes:\na hybrid method for application in forensic analysis","volume":"201","author":"Arrieiro","year":"2024","journal-title":"Microchem. 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Nano Mater."},{"key":"2026081508103442200_cit35","doi-asserted-by":"publisher","first-page":"117454","DOI":"10.1016\/j.bios.2025.117454","article-title":"Ion-imprinting strategy towards a\nnovel two-in-one copper-based nanozyme for sensitive electrochemical\u2013colorimetric\ndual-mode detection of paracetamol","volume":"280","author":"Liao","year":"2025","journal-title":"Biosens.\nBioelectron."},{"key":"2026081508103442200_cit36","doi-asserted-by":"publisher","DOI":"10.1351\/goldbook.L03540","volume-title":"Compendium of Chemical Terminology (the IUPAC\nGold Book)","author":"IUPAC","year":"2014"},{"key":"2026081508103442200_cit37","unstructured":"Chemicalize Instant Cheminformatics Solutions. https:\/\/chemicalize.com\/app\/calculation (accessed Nov 11, 2024)."},{"key":"2026081508103442200_cit38","doi-asserted-by":"publisher","first-page":"535","DOI":"10.1046\/j.1365-2125.2002.01564.x","article-title":"Concomitant overdosing\nof other drugs in patients with\nparacetamol poisoning","volume":"53","author":"Schmidt","year":"2002","journal-title":"Br. J. Clin. Pharmacol."},{"key":"2026081508103442200_cit39","doi-asserted-by":"publisher","first-page":"298","DOI":"10.1002\/elan.1140070324","article-title":"Cyclic voltammetry-simulation\nand analysis of reaction mechanisms","volume":"7","author":"Beck","year":"1995","journal-title":"Electroanalysis"},{"key":"2026081508103442200_cit40","doi-asserted-by":"publisher","first-page":"236","DOI":"10.1111\/j.1753-4887.1976.tb05776.x","article-title":"Vitamin C toxicity","volume":"34","author":"Clinic","year":"1976","journal-title":"Nutr. Rev."},{"key":"2026081508103442200_cit41","unstructured":"National Library of Medicine. Hyperuricemia; National Center for Biotechnology Information, 2023. https:\/\/www.ncbi.nlm.nih.gov\/books\/NBK459218\/ (accessed November\n19, 2024)."},{"key":"2026081508103442200_cit42","unstructured":"Mayo Clinic Laboratories\n          . CAFF-Overview: Caffeine, Serum. https:\/\/www.mayocliniclabs.com\/test-catalog\/Overview\/8754 (accessed\nNovember 19, 2024)."},{"key":"2026081508103442200_cit43","unstructured":"Cleveland Clinic.\nBlood glucose (sugar) test: levels and what they\nmean. https:\/\/my.clevelandclinic.org\/health\/diagnostics\/12363-blood-glucose-test (accessed November 19, 2024)."}],"container-title":["ACS Omega"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsomega.5c12147","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/acsodf\/article-pdf\/11\/9\/15094\/63273449\/ao5c12147.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/acsodf\/article-pdf\/11\/9\/15094\/63273449\/ao5c12147.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T13:51:55Z","timestamp":1786801915000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/acsodf\/article\/11\/9\/15094\/5079178\/Rapid-Point-of-Care-Assessment-of-Paracetamol"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,24]]},"references-count":43,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,2,24]]},"published-print":{"date-parts":[[2026,3,10]]}},"URL":"https:\/\/doi.org\/10.1021\/acsomega.5c12147","relation":{},"ISSN":["2470-1343"],"issn-type":[{"value":"2470-1343","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,24]]}}}