{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T12:12:56Z","timestamp":1788437576056,"version":"build-2803163510"},"reference-count":45,"publisher":"American Chemical Society (ACS)","issue":"34","license":[{"start":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:00:00Z","timestamp":1787011200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22404006"],"award-info":[{"award-number":["22404006"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2024QB407"],"award-info":[{"award-number":["ZR2024QB407"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007322","name":"Binzhou Medical University","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/501100007322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/na","name":"Yantai Science and Technology Innovation Development Plan Project","doi-asserted-by":"publisher","award":["2024JCYJ090"],"award-info":[{"award-number":["2024JCYJ090"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Curcumin, as an example of a lipophilic bioactive compound, is typically ingested orally for absorption into the human body. However, dynamically tracking the interaction between lipophilic bioactive compounds and digestive enzymes remains a significant challenge in the study of lipophilic bioactive compounds\u2019 absorption, translocation, and metabolism within the gastrointestinal tract. The interaction between curcumin and lipase or trypsin was monitored in real time using an ordered porous layer interference (OPLI) system based on silica colloidal crystal (SCC) films. The trypsin or lipase layers were obtained by adsorbing trypsin or lipase onto the SCC films. The optical thickness (OT) received by the OPLI system was used to monitor the interaction between curcumin and digestive enzymes in real time. By comparing the interaction processes of curcumin on trypsin layers at different pH values, it was determined that the structure of curcumin under varying pH conditions influenced its interaction with trypsin. The results for changes in optical thickness (\u0394OT) showed that the \u0394OT of curcumin was greater in the trypsin (24.79 \u00b1 4.94 nm) than in the lipase (2.49 \u00b1 0.84 nm). The combination or dissociation rate constant (Kon or Koff) of curcumin in the presence of the trypsin and lipase layers was compared and analyzed through \u0394OT. The calculated results revealed that the affinity constant (KA) of curcumin on the trypsin layer and the lipase layer were 1.15 \u00d7 103 M\u20131 and 5.33 \u00d7 103 M\u20131, respectively. Analyzing the association and dissociation of curcumin with digestive enzymes offers real-time information and dynamic parameters for a more profound comprehension of the behavior of lipid-soluble drugs in the gastrointestinal tract.<\/jats:p>","DOI":"10.1021\/acsomega.5c13419","type":"journal-article","created":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T16:13:13Z","timestamp":1787069593000},"page":"50751-50761","source":"Crossref","is-referenced-by-count":0,"title":["Investigating the\nInteraction between a Lipophilic\nBioactive Compound and Digestive Enzymes via Interference Effects\nin Colloidal Crystal Films"],"prefix":"10.1021","volume":"11","author":[{"given":"Haixin","family":"Bian","sequence":"first","affiliation":[{"name":"Shandong Medical and Pharmaceutical University , , ,","place":["Yantai, China, 264003"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaonan","family":"Cao","sequence":"additional","affiliation":[{"name":"Shandong Medical and Pharmaceutical University , , ,","place":["Yantai, China, 264003"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shandong Medical and Pharmaceutical University , , ,","place":["Yantai, China, 264003"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-1177-5223","authenticated-orcid":true,"given":"Lele","family":"Zhou","sequence":"additional","affiliation":[{"name":"Shandong Medical and Pharmaceutical University , , ,","place":["Yantai, China, 264003"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,18]]},"reference":[{"key":"2026090307162646200_cit1","doi-asserted-by":"publisher","first-page":"307","DOI":"10.3892\/ol.2023.13893","article-title":"Curcumin inhibits\nproliferation of hepatocellular carcinoma cells\nby blocking PTPN1 and PTPN11 expression","volume":"26","author":"Zhang","year":"2023","journal-title":"Oncol.\nLett."},{"key":"2026090307162646200_cit2","doi-asserted-by":"publisher","first-page":"1249418","DOI":"10.3389\/fphar.2023.1249418","article-title":"Protective effects\nof curcumin against osteoporosis and its molecular mechanisms: a recent\nreview in preclinical trials","volume":"14","author":"Yang","year":"2023","journal-title":"Front. 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Soc."},{"key":"2026090307162646200_cit6","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1007\/s11483-024-09855-5","article-title":"Delivery of Curcumin\nUsing Colloidal Complexes Formed from Ammonium\nChitosan and Acid Treated Soybean Protein","volume":"19","author":"Weng","year":"2024","journal-title":"Food\nBiophys."},{"key":"2026090307162646200_cit7","doi-asserted-by":"publisher","first-page":"199","DOI":"10.3390\/separations11070199","article-title":"Extraction of Bioactive\nCompound-Rich Essential Oil from Cistus ladanifer L. by Microwave-Assisted\nHydrodistillation: GC-MS Characterization, In Vitro Pharmacological\nActivities, and Molecular Docking","volume":"11","author":"El Hachlafi","year":"2024","journal-title":"Separations"},{"issue":"8","key":"2026090307162646200_cit8","doi-asserted-by":"publisher","DOI":"10.7759\/cureus.67870","article-title":"Molecular Docking and Pharmacokinetics\nPrediction of Piperine and Capsaicin as Human Pancreatic Lipase Inhibitors:\nAn In Silico Study","volume":"16","author":"S","year":"2024","journal-title":"Cureus"},{"key":"2026090307162646200_cit9","doi-asserted-by":"publisher","first-page":"46","DOI":"10.3390\/bios7040046","article-title":"Development and Bioanalytical\nApplications of a White\nLight Reflectance Spectroscopy Label-Free Sensing Platform","volume":"7","author":"Koukouvinos","year":"2017","journal-title":"Biosensors"},{"key":"2026090307162646200_cit10","doi-asserted-by":"publisher","first-page":"7832","DOI":"10.1021\/ja101415b","article-title":"Metal\u2013Organic\nFrameworks as Sensors: A ZIF-8\nBased Fabry\u2013Pe\u0301rot Device as a Selective Sensor for Chemical\nVapors and Gases","volume":"132","author":"Lu","year":"2010","journal-title":"J. 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Macromol."},{"key":"2026090307162646200_cit29","doi-asserted-by":"publisher","first-page":"13482","DOI":"10.1021\/acs.analchem.4c01646","article-title":"Development\nof a Methodology Based on Optical Interferometry for\nMeasuring Fibrinolytic Activity","volume":"96","author":"Liu","year":"2024","journal-title":"Anal. Chem."},{"key":"2026090307162646200_cit30","doi-asserted-by":"publisher","first-page":"14413","DOI":"10.1021\/acs.analchem.4c01808","article-title":"Real-Time Study of\nthe Specific Interactions of Lactoferrin with\nMimicked Heparan Sulfate Meshes Using Ordered Porous Layer Interferometry","volume":"96","author":"Zhang","year":"2024","journal-title":"Anal. Chem."},{"key":"2026090307162646200_cit31","doi-asserted-by":"publisher","first-page":"77","DOI":"10.3329\/bjp.v9i1.17474","article-title":"Molecular docking\nanalysis of curcumin analogues as human neutrophil elastase inhibitors","volume":"9","author":"Narayanaswamy","year":"2014","journal-title":"Bangladesh J. 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Food Res."},{"key":"2026090307162646200_cit39","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1007\/s11483-021-09697-5","article-title":"Molecular Interactions\nof Pea Globulin, Albumin and\nGlutelin With Curcumin: Formation and Gastric Release Mechanisms of\nCurcumin-loaded Bio-nanocomplexes","volume":"17","author":"Okagu","year":"2022","journal-title":"Food Biophys."},{"key":"2026090307162646200_cit40","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1016\/j.foodhyd.2018.12.040","article-title":"Heat-induced self-assembly\nof zein nanoparticles: fabrication, stabilization\nand potential application as oral drug delivery","volume":"90","author":"Wang","year":"2019","journal-title":"Food Hydrocolloids"},{"key":"2026090307162646200_cit41","doi-asserted-by":"publisher","first-page":"144288","DOI":"10.1016\/j.foodchem.2025.144288","article-title":"Real-time detection of plasmin activity\nin milk using an ordered\nporous layer interferometry","volume":"483","author":"Liu","year":"2025","journal-title":"Food Chem."},{"key":"2026090307162646200_cit42","doi-asserted-by":"publisher","first-page":"340582","DOI":"10.1016\/j.aca.2022.340582","article-title":"Monitoring of silica\ncolloidal crystal-embedded chitosan\nhydrogel films swelling and its drug release application","volume":"1236","author":"Yan","year":"2022","journal-title":"Anal. 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