{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:13:03Z","timestamp":1788221583554,"version":"build-2803163510"},"reference-count":41,"publisher":"American Chemical Society (ACS)","issue":"33","license":[{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010661","name":"Horizon 2020 Framework Programme","doi-asserted-by":"publisher","award":["101129734"],"award-info":[{"award-number":["101129734"]}],"id":[{"id":"10.13039\/100010661","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Extracellular vesicles (EVs) play crucial roles in intercellular communication and represent promising therapeutic vehicles, yet current isolation and manipulation methods often irreversibly alter their properties. This study presents a charge-mediated approach for the reversible interaction and release of EVs using Giant Unilamellar Vesicles (GUVs) exploiting tailored surface charges. Negatively charged EVs can be selectively bound through electrostatic interactions with positively charged GUVs, forming stable aggregates. Critically, these interactions are reversible upon the introduction of a negatively charged moiety, oleic acid, which disaggregates the complexes and releases the vesicles. Selective fusion events between oppositely charged GUVs allow modulation of internal composition, while GUVs\u2013EVs interactions remain nonfusogenic. This reversible, charge-based platform offers advantages over current EVs\u2019 capture methods, including simplicity, low cost, and the potential for a separation method without permanent labeling.<\/jats:p>","DOI":"10.1021\/acsomega.6c00387","type":"journal-article","created":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T08:01:54Z","timestamp":1786608114000},"page":"49033-49044","source":"Crossref","is-referenced-by-count":0,"title":["Reversible Charge-Mediated\nCapture and Release of\nExtracellular Vesicles Using Giant Unilamellar Vesicles"],"prefix":"10.1021","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8045-8772","authenticated-orcid":true,"given":"Marinella","family":"Pinelli","sequence":"first","affiliation":[{"name":"University of Trento , , Via Sommarive 9 , , ,","place":["Povo, Italy, 38123"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriele","family":"Schiavi","sequence":"additional","affiliation":[{"name":"University of Trento , , Via Sommarive 9 , , ,","place":["Povo, Italy, 38123"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dalia","family":"Tarantino","sequence":"additional","affiliation":[{"name":"University of Trento , , Via Sommarive 9 , , ,","place":["Povo, Italy, 38123"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"Valer","sequence":"additional","affiliation":[{"name":"University of Trento , , Via Sommarive 9 , , ,","place":["Povo, Italy, 38123"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Cortese","sequence":"additional","affiliation":[{"name":"University of Trento , , Via Sommarive 9 , , ,","place":["Povo, Italy, 38123"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michela","family":"Notarangelo","sequence":"additional","affiliation":[{"name":"University of Trento , , Via Sommarive 9 , , ,","place":["Povo, Italy, 38123"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miriam","family":"Romano","sequence":"additional","affiliation":[{"name":"University of Brescia , , ,","place":["Brescia, Italy, 25123"]},{"name":"Center for Colloid and Surface Science (CSGI) , ,","place":["Sesto Fiorentino (FI), Italy, 50019"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2737-1090","authenticated-orcid":true,"given":"Annalisa","family":"Radeghieri","sequence":"additional","affiliation":[{"name":"University of Brescia , , ,","place":["Brescia, Italy, 25123"]},{"name":"Center for Colloid and Surface Science (CSGI) , ,","place":["Sesto Fiorentino (FI), Italy, 50019"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4410-5272","authenticated-orcid":true,"given":"Lucia","family":"Paolini","sequence":"additional","affiliation":[{"name":"Center for Colloid and Surface Science (CSGI) , ,","place":["Sesto Fiorentino (FI), Italy, 50019"]},{"name":"University of Brescia , , ,","place":["Brescia, Italy, 25123"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4652-2168","authenticated-orcid":true,"given":"Paolo","family":"Bergese","sequence":"additional","affiliation":[{"name":"University of Brescia , , ,","place":["Brescia, Italy, 25123"]},{"name":"Center for Colloid and Surface Science (CSGI) , ,","place":["Sesto Fiorentino (FI), Italy, 50019"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vito G.","family":"D\u2019Agostino","sequence":"additional","affiliation":[{"name":"University of Trento , , Via Sommarive 9 , , ,","place":["Povo, Italy, 38123"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5453-2139","authenticated-orcid":true,"given":"Martin M.","family":"Hanczyc","sequence":"additional","affiliation":[{"name":"University of Trento , , Via Sommarive 9 , , ,","place":["Povo, Italy, 38123"]},{"name":"University of New Mexico , , , ,","place":["Albuquerque, New Mexico, United States, 87106"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2572-8677","authenticated-orcid":true,"given":"Silvia","family":"Holler","sequence":"additional","affiliation":[{"name":"University of Trento , , Via Sommarive 9 , , ,","place":["Povo, Italy, 38123"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,13]]},"reference":[{"key":"2026083119134017700_cit1","volume-title":"Histology, Extracellular Vesicles","author":"Ibrahim","year":"2025"},{"key":"2026083119134017700_cit2","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1186\/s12964-023-01103-6","article-title":"A review of the regulatory\nmechanisms of extracellular vesicles-mediated\nintercellular communication","volume":"21","author":"Liu","year":"2023","journal-title":"Cell Commun. 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