{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T08:02:33Z","timestamp":1793779353764,"version":"4.6.1"},"reference-count":45,"publisher":"American Chemical Society (ACS)","issue":"33","license":[{"start":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T00:00:00Z","timestamp":1786320000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010628","name":"Universidad de La Sabana","doi-asserted-by":"publisher","award":["INGPHD-54-2023"],"award-info":[{"award-number":["INGPHD-54-2023"]}],"id":[{"id":"10.13039\/501100010628","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Wound healing is a dynamic process that consists of four stages: hemostasis, inflammation, proliferation, and remodeling. Some alterations in this process can lead to fatal adverse effects such as bleeding or chronic wound formation. Thus, the use of appropriate dressings is necessary to allow for hemostasis and healing to take place properly. Nonisocyanate polyhydroxyurethanes (NIPHUs) have emerged as a more sustainable alternative for biomaterials. Thus, the aim was to evaluate the wound healing capacity of four new NIPHUs with different ratios of amines used. The water vapor transmission rate (WVTR), in vivo hemostatic capability in the mouse tail model, skin irritation, and wound healing assays were performed. Results showed new formulations of NIPHUs with different WVTRs (95.6 g\/m2\/day to 593.3 g\/m2\/day). The amount of blood loss was lower when NIPHU 50\/50 was applied than in wounds without any treatment. NIPHUs that were nonirritating dressings were used in the healing process assays. On day 14, all of the wounds were closed, including controls. The healed zone in animals treated with any NIPHUs was smaller and thinner, while an elongated and thick scab was still observed in the healed skin of the control groups (wounds without any treatment and wounds treated with a commercial patch). In accordance with the macroscopic observations, in all treatments, the histologic observations showed that the healing zone was epithelialized, and below it was occupied by residual granulation tissue. In NIPHU 100\/0, NIPHU 67\/33, and controls, no hypodermis was identified, but in the case of NIPHU 50\/50, below this epithelium, several regions could be recognized: connective tissue with active fibroblasts was observed, and there were some newly formed adipocytes, neoformation of pilosebaceous units, and the muscle layer was complete. In conclusion, as a preliminary study, NIPHU 50\/50 exhibited hemostatic activity and allowed the wounds to be in the remodeling phase in 14 days, making it suitable for use as a dressing.<\/jats:p>","DOI":"10.1021\/acsomega.6c01304","type":"journal-article","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T18:02:11Z","timestamp":1786384931000},"page":"49127-49138","source":"Crossref","is-referenced-by-count":1,"title":["Hemostatic and\nWound Healing Nonisocyanate Polyhydroxyurethanes\n(NIPHUs) Dressings from Soybean Oil:\n                    <i>In Vivo<\/i>\n                    Characterization"],"prefix":"10.1021","volume":"11","author":[{"given":"Maria Paula","family":"Morales-Gonzalez","sequence":"first","affiliation":[{"name":"Universidad de La Sabana , , ,","place":["Chi\u0301a, Colombia, 140013"]},{"name":"Universidad de La Sabana , , ,","place":["Chi\u0301a, Colombia, 140013"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel F.","family":"Valero","sequence":"additional","affiliation":[{"name":"Universidad de La Sabana , , ,","place":["Chi\u0301a, Colombia, 140013"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9588-9935","authenticated-orcid":true,"given":"Luis Eduardo","family":"Di\u0301az","sequence":"additional","affiliation":[{"name":"Universidad de La Sabana , , ,","place":["Chi\u0301a, Colombia, 140013"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nallely","family":"A\u0301lvarez-Santos","sequence":"additional","affiliation":[{"name":"National Autonomous University of Mexico (UNAM) , , , ,","place":["Tlalnepantla de Baz, Mexico State, Mexico, 54090"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana Montserrat","family":"Espinosa-Gonza\u0301lez","sequence":"additional","affiliation":[{"name":"National Autonomous University of Mexico (UNAM) , , , ,","place":["Tlalnepantla de Baz, Mexico State, Mexico, 54090"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose\u0301 Del Carmen","family":"Beni\u0301tez-Flores","sequence":"additional","affiliation":[{"name":"National Autonomous University of Mexico (UNAM) , , , ,","place":["Tlalnepantla de Baz, Mexico State, Mexico, 54090"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose\u0301 Guillermo","family":"Avila-Acevedo","sequence":"additional","affiliation":[{"name":"National Autonomous University of Mexico (UNAM) , , , ,","place":["Tlalnepantla de Baz, Mexico State, Mexico, 54090"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1570-8728","authenticated-orcid":true,"given":"Ana Mari\u0301a","family":"Garci\u0301a-Bores","sequence":"additional","affiliation":[{"name":"National Autonomous University of Mexico (UNAM) , , , ,","place":["Tlalnepantla de Baz, Mexico State, Mexico, 54090"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,10]]},"reference":[{"issue":"21","key":"2026083119112539500_cit1","doi-asserted-by":"publisher","first-page":"4301","DOI":"10.3390\/polym15214301","article-title":"An Advanced Review: Polyurethane-Related\nDressings for Skin Wound Repair","volume":"15","author":"Liang","year":"2023","journal-title":"Polymers"},{"issue":"13","key":"2026083119112539500_cit2","doi-asserted-by":"publisher","first-page":"5264","DOI":"10.3390\/molecules28135264","article-title":"Hemostasis Strategies and Recent\nAdvances in Nanomaterials for Hemostasis","volume":"28","author":"Du","year":"2023","journal-title":"Molecules"},{"issue":"3","key":"2026083119112539500_cit3","doi-asserted-by":"publisher","first-page":"90","DOI":"10.3390\/cosmetics11030090","article-title":"Disturbances in the Skin Homeostasis:\nWound Healing, an Undefined Process","volume":"11","author":"Fe\u0301rnandez-Guarino","year":"2024","journal-title":"Cosmetics"},{"issue":"4","key":"2026083119112539500_cit4","doi-asserted-by":"publisher","first-page":"326","DOI":"10.3109\/10409238.2015.1050550","article-title":"How It\nAll Starts: Initiation of\nthe Clotting Cascade","volume":"50","author":"Smith","year":"2015","journal-title":"Crit. Rev. Biochem. Mol.\nBiol."},{"issue":"2","key":"2026083119112539500_cit5","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1177\/08839115221147935","article-title":"Current Status\nof Hemostatic Agents,\nTheir Mechanism of Action, and Future Directions","volume":"38","author":"Gupta","year":"2023","journal-title":"J. Bioact. Compat. Polym."},{"key":"2026083119112539500_cit6","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1098\/rsob.200223","article-title":"Wound Healing: Cellular\nMechanisms and Pathological\nOutcomes","volume":"10","author":"Wilkinson","year":"2020","journal-title":"Open Biol."},{"issue":"1","key":"2026083119112539500_cit7","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1080\/09546634.2020.1730296","article-title":"Therapeutic Advances\nin Wound Healing","volume":"33","author":"Oliveira","year":"2022","journal-title":"J. Dermatol. Treat."},{"key":"2026083119112539500_cit8","doi-asserted-by":"publisher","first-page":"3706315","DOI":"10.1155\/2019\/3706315","article-title":"Skin\nAcute Wound Healing: A Comprehensive Review","volume":"2019","author":"Can\u0303edo-Dorantes","year":"2019"},{"key":"2026083119112539500_cit9","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1016\/j.addr.2018.12.014","article-title":"Advanced\nDrug Delivery Systems and Artificial Skin Grafts for Skin Wound Healing","volume":"146","author":"Kim","year":"2019","journal-title":"Adv. Drug Delivery Rev."},{"issue":"1","key":"2026083119112539500_cit10","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1016\/j.mpsur.2021.11.003","article-title":"The Pathology\nof Healing and Repair","volume":"40","author":"Carr","year":"2022","journal-title":"Surgery"},{"key":"2026083119112539500_cit11","doi-asserted-by":"publisher","first-page":"101290","DOI":"10.1016\/j.nantod.2021.101290","article-title":"Smart Wound Dressings\nfor Wound Healing","volume":"41","author":"Dong","year":"2021","journal-title":"Nano\nToday"},{"key":"2026083119112539500_cit12","doi-asserted-by":"publisher","first-page":"100300","DOI":"10.1016\/j.giant.2024.100300","article-title":"Skin-like Breathable\nWound Dressings with Antimicrobial and Hemostatic Properties","volume":"19","author":"Wu","year":"2024","journal-title":"Giant"},{"issue":"3","key":"2026083119112539500_cit13","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1016\/0195-6701(87)90011-9","article-title":"\u2018Tegaderm\u2019\nDressings Prevent Recolonization\nof Chlorhexidine-Treated Skin","volume":"10","author":"Holmstro\u0308m","year":"1987","journal-title":"J. Hosp. Infect."},{"issue":"6","key":"2026083119112539500_cit14","doi-asserted-by":"publisher","first-page":"2945","DOI":"10.1007\/s00289-019-02891-z","article-title":"Wound Dressing\nApplication of Castor Oil- and CAPA-Based Polyurethane Membranes","volume":"77","author":"Rezaei Hosseinabadi","year":"2020","journal-title":"Polym. Bull."},{"issue":"4","key":"2026083119112539500_cit15","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1088\/1748-605X\/ab7387","article-title":"Vegetable Oil-Based\nPolyurethanes as Antimicrobial\nWound Dressings: In Vitro and in Vivo Evaluation","volume":"15","author":"Gholami","year":"2020","journal-title":"Biomed. Mater."},{"issue":"6","key":"2026083119112539500_cit16","doi-asserted-by":"publisher","first-page":"3233","DOI":"10.1007\/s00289-018-2531-7","article-title":"Advances in\nthe Synthesis of Non-Isocyanate\nPolyurethanes","volume":"76","author":"Suryawanshi","year":"2019","journal-title":"Polym. Bull."},{"key":"2026083119112539500_cit17","doi-asserted-by":"publisher","first-page":"127446","DOI":"10.1016\/j.polymer.2024.127446","article-title":"Biodegradable and Biocompatible Nonisocyanate Polyurethanes Synthesized\nfrom Bio-Derived Precursors","volume":"309","author":"Prasannatha","year":"2024","journal-title":"Polymer"},{"key":"2026083119112539500_cit18","doi-asserted-by":"publisher","first-page":"128553","DOI":"10.1016\/j.polymer.2025.128553","article-title":"Beyond\nIsocyanates: Advances in Non-Isocyanate\nPolyurethane Chemistry and Applications","volume":"332","author":"Chaudhary","year":"2025","journal-title":"Polymer"},{"key":"2026083119112539500_cit19","doi-asserted-by":"publisher","first-page":"113985","DOI":"10.1016\/j.eurpolymj.2025.113985","article-title":"Non-Isocyanate Polyurethanes:\nPerspectives as Biomaterials","volume":"233","author":"Pierrard","year":"2025","journal-title":"Eur. Polym. J."},{"issue":"13","key":"2026083119112539500_cit20","doi-asserted-by":"publisher","first-page":"9273","DOI":"10.1039\/D3RA08684D","article-title":"A Review on\nVegetable Oil-Based Non\nIsocyanate Polyurethane: Towards a Greener and Sustainable Production\nRoute","volume":"14","author":"Rayung","year":"2024","journal-title":"RSC Adv."},{"key":"2026083119112539500_cit21","doi-asserted-by":"publisher","first-page":"110142","DOI":"10.1016\/j.eurpolymj.2020.110142","article-title":"Soybean Oil-Derived\nNon-Isocyanate Polyurethanes Containing\nAzetidinium Groups as Antibacterial Wound Dressing Membranes","volume":"142","author":"Gholami","year":"2021","journal-title":"Eur. Polym. J."},{"issue":"13","key":"2026083119112539500_cit22","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e33693","article-title":"Non-Isocyanate Polyurethane-Co-Polyglycolic\nAcid Electrospun Nanofiber Membrane Wound Dressing with High Biocompatibility,\nHemostasis, and Prevention of Chronic Wound Formation","volume":"10","author":"Ge","year":"2024","journal-title":"Heliyon"},{"key":"2026083119112539500_cit23","doi-asserted-by":"publisher","first-page":"138733","DOI":"10.1016\/j.ijbiomac.2024.138733","volume":"288","author":"Quan","year":"2025","journal-title":"Int. J. Biol. 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Eng."},{"issue":"1","key":"2026083119112539500_cit27","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1002\/jbm.a.34672","article-title":"Preparation and Characterization\nof Novel Antibacterial Castor Oil-Based Polyurethane Membranes for\nWound Dressing Application","volume":"102","author":"Yari","year":"2014","journal-title":"J. Biomed. Mater.\nRes., Part A"},{"key":"2026083119112539500_cit28","article-title":"OECD Guidelines for the Testing of\nChemicals: Acute Dermal Irritation\/Corrosion","author":"OECD.","year":"2015"},{"issue":"1","key":"2026083119112539500_cit29","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1007\/s12668-024-01708-2","article-title":"Optimization\nof Chemical Synthesis for Production of a Skin-Compatible and Scalable\nCopper Oxide Nanoparticles: A Promising and Safe Strategy for Healing\nof Non-Infected and Infected Wounds in Diabetes","volume":"15","author":"Ali","year":"2025","journal-title":"BioNanoscience"},{"key":"2026083119112539500_cit30","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2024.114378","article-title":"Comprehensive Assessment of Intradermal Responses to\nHyaluronic Acid-Based\nSkin Injection Fillers through Multi-Pathway Dynamic Synergies","volume":"246","author":"Zhong","year":"2025","journal-title":"Colloids Surf. B Biointerfaces"},{"issue":"6","key":"2026083119112539500_cit31","doi-asserted-by":"publisher","first-page":"863","DOI":"10.3390\/polym16060863","article-title":"Highly Efficient\nHemostatic\nCross-Linked Polyacrylate Polymer Dressings for Immediate Hemostasis","volume":"16","author":"Ye","year":"2024","journal-title":"Polymers"},{"issue":"14","key":"2026083119112539500_cit32","doi-asserted-by":"publisher","first-page":"11831","DOI":"10.3390\/ijms241411831","article-title":"Anti-Inflammatory\nand Histological Analysis of Skin Wound Healing through Topical Application\nof Mexican Propolis","volume":"24","author":"Balderas-Cordero","year":"2023","journal-title":"Int. J. Mol. Sci."},{"key":"2026083119112539500_cit33","doi-asserted-by":"publisher","first-page":"102150","DOI":"10.1016\/j.fbio.2022.102150","article-title":"Asterohyptis\nStellulata: Phytochemistry and Wound Healing Activity","volume":"50","author":"A\u0301lvarez-Santos","year":"2022","journal-title":"Food Biosci."},{"issue":"1","key":"2026083119112539500_cit34","doi-asserted-by":"publisher","first-page":"335","DOI":"10.3390\/CIMB43010028","article-title":"Histological Evidence of Wound Healing\nImprovement in Rats Treated with Oral Administration of Hydroalcoholic\nExtract of Vitis Labrusca","volume":"43","author":"Santos","year":"2021","journal-title":"Curr. Issues Mol.\nBiol."},{"issue":"1","key":"2026083119112539500_cit35","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s10856-023-06774-8","article-title":"Development\nof Collagenous Scaffolds for Wound Healing: Characterization and in\nVivo Analysis","volume":"35","author":"Rodrigues","year":"2024","journal-title":"J. Mater. Sci.: Mater. Med."},{"issue":"12","key":"2026083119112539500_cit36","doi-asserted-by":"publisher","first-page":"727","DOI":"10.3390\/polym9120727","article-title":"Preparation and Characterization of Breathable Hemostatic\nHydrogel Dressings and Determination of Their Effects on Full-Thickness\nDefects","volume":"9","author":"Pan","year":"2017","journal-title":"Polymers"},{"key":"2026083119112539500_cit37","doi-asserted-by":"publisher","DOI":"10.1093\/burnst\/tkac024","article-title":"Commercial Wound Dressings\nfor the Treatment of Exuding Wounds: An in-Depth Physico-Chemical\nComparative Study","volume":"10","author":"Minsart","year":"2022","journal-title":"Burns Trauma"},{"issue":"2","key":"2026083119112539500_cit38","doi-asserted-by":"publisher","first-page":"282","DOI":"10.3390\/polym12020282","article-title":"Water Vapor\nPermeability through Porous Polymeric Membranes with Various Hydrophilicity\nas Synthetic and Natural Barriers","volume":"12","author":"Anatoly","year":"2020","journal-title":"Polymers"},{"issue":"20","key":"2026083119112539500_cit39","doi-asserted-by":"publisher","first-page":"5575","DOI":"10.1016\/S0032-3861(98)00766-6","article-title":"Hydrophilic\nPolyurethaneurea Membranes: Influence of\nSoft Block Composition on the Water Vapor Permeation Rates","volume":"40","author":"Yilgo\u0308r","year":"1999","journal-title":"Polymer"},{"key":"2026083119112539500_cit40","doi-asserted-by":"publisher","first-page":"119371","DOI":"10.1016\/j.saa.2020.119371","article-title":"Time Series Fourier\nTransform Infrared Spectroscopy\nfor Characterization of Water Vapor Sorption in Hydrophilic and Hydrophobic\nPolymeric Films","volume":"250","author":"Xu","year":"2021","journal-title":"Spectrochim. Acta, Part A"},{"issue":"12","key":"2026083119112539500_cit41","doi-asserted-by":"publisher","first-page":"685","DOI":"10.1089\/wound.2020.1232","article-title":"Moist Wound Healing\nwith Commonly Available Dressings","volume":"10","author":"Nuutila","year":"2021","journal-title":"Adv. Wound\nCare"},{"issue":"4","key":"2026083119112539500_cit42","doi-asserted-by":"publisher","first-page":"042006","DOI":"10.1088\/2516-1091\/ac23a4","article-title":"Synthetic Hydrogels\nas Blood Clot Mimicking Wound Healing Materials","volume":"3","author":"Rausch","year":"2021","journal-title":"Prog. Biomed. Eng."},{"key":"2026083119112539500_cit43","doi-asserted-by":"publisher","first-page":"136395","DOI":"10.1016\/j.ijbiomac.2024.136395","article-title":"Hyaluronic Acid-Based\n\u03b5-Polylysine\/Polyurethane Asymmetric Sponge for Enhanced Wound\nHealing","volume":"281","author":"Niu","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"2026083119112539500_cit44","doi-asserted-by":"publisher","first-page":"103563","DOI":"10.1016\/j.cis.2025.103563","article-title":"Advances in\nBiomaterials for Wound\nCare Management: Insights from Recent Developments","volume":"343","author":"Joshi","year":"2025","journal-title":"Adv. Colloid Interface Sci."},{"key":"2026083119112539500_cit45","doi-asserted-by":"publisher","DOI":"10.1016\/j.hybadv.2025.100381","article-title":"Harnessing\nNatural Polymers and Nanoparticles:\nSynergistic Scaffold Design for Improved Wound Healing","volume":"8","author":"Anitha","year":"2025","journal-title":"Hybrid Adv."}],"container-title":["ACS Omega"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsomega.6c01304","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/acsodf\/article-pdf\/11\/33\/49127\/66479076\/acsomega.6c01304.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/acsodf\/article-pdf\/11\/33\/49127\/66479076\/acsomega.6c01304.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T23:11:33Z","timestamp":1788217893000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/acsodf\/article\/11\/33\/49127\/5251565\/Hemostatic-and-Wound-Healing-Nonisocyanate"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,10]]},"references-count":45,"journal-issue":{"issue":"33","published-online":{"date-parts":[[2026,8,10]]},"published-print":{"date-parts":[[2026,8,25]]}},"URL":"https:\/\/doi.org\/10.1021\/acsomega.6c01304","relation":{},"ISSN":["2470-1343"],"issn-type":[{"value":"2470-1343","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,10]]}}}