{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T16:11:04Z","timestamp":1790093464116,"version":"4.0.1"},"reference-count":42,"publisher":"American Chemical Society (ACS)","issue":"37","license":[{"start":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T00:00:00Z","timestamp":1788739200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004901","name":"Funda??o de Amparo ? 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In this study, the interaction between \u03b1-lactalbumin (\u03b1La) and gold nanoparticles (AuNPs) functionalized with different surface ligands (citrate, 3-mercaptopropionic acid (MPA), and polyethylene glycol (PEG 6000)) was investigated by surface plasmon resonance (SPR). Real-time monitoring enabled determination of association and dissociation rate constants, residence times, and activation parameters over a controlled temperature range (285.2\u2013301.2 K). Adsorption proceeded under a rapid kinetic regime for all systems, with both association (107\u2013108 M\u20131 s\u20131) and dissociation (0.5 s\u20131) occurring on short time scales. Despite this overall similarity, the magnitude and thermal dependence of kinetic parameters were strongly influenced by ligand chemistry, with association rates and residence times following the order PEG &amp;gt; MPA \u2248 citrate. Activation analyses indicated that formation of the transition state possibly involves contributions from interfacial desolvation and counterion release. For citrate-coated nanoparticles, temperature-dependent parameters were obtained, suggesting the involvement of conformational changes on \u03b1La upon binding. These findings provide mechanistic insights into how surface functionalization governs the kinetic and energetic pathways of protein adsorption at gold nanobiointerfaces.<\/jats:p>","DOI":"10.1021\/acsomega.6c01893","type":"journal-article","created":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T06:01:34Z","timestamp":1788760894000},"page":"55082-55092","source":"Crossref","is-referenced-by-count":0,"title":["Surface Ligand-Dependent\nKinetics of \u03b1-Lactalbumin\nInteraction with Gold Nanoparticles Investigated by SPR"],"prefix":"10.1021","volume":"11","author":[{"suffix":"Jr.","given":"Carlos Anto\u0302nio","family":"Ferraz","sequence":"first","affiliation":[{"name":"Universidade Federal de Alfenas , , ,","place":["Alfenas, Brasil, 37130-000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6376-4812","authenticated-orcid":true,"given":"Isabela Araujo","family":"Marques","sequence":"additional","affiliation":[{"name":"Universidade Federal de Vic\u0327osa , , ,","place":["Vic\u0327osa, Brasil, 36570-000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-8816-1152","authenticated-orcid":true,"given":"Thalles Maranesi","family":"Pereira","sequence":"additional","affiliation":[{"name":"Universidade Federal de Alfenas , , ,","place":["Alfenas, Brasil, 37130-000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ygor","family":"Ribeiro Guimara\u0303es","sequence":"additional","affiliation":[{"name":"Universidade Federal de Vic\u0327osa , , ,","place":["Vic\u0327osa, Brasil, 36570-000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ci\u0301nthia","family":"das Dores Aguiar","sequence":"additional","affiliation":[{"name":"Universidade Federal de Alfenas , , ,","place":["Alfenas, Brasil, 37130-000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hauster Maximiler","family":"Campos de Paula","sequence":"additional","affiliation":[{"name":"Universidade Federal de Vic\u0327osa , , ,","place":["Vic\u0327osa, Brasil, 36570-000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9814-1394","authenticated-orcid":true,"given":"Luciano Sindra","family":"Virtuoso","sequence":"additional","affiliation":[{"name":"Universidade Federal de Alfenas , , ,","place":["Alfenas, Brasil, 37130-000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nelson Henrique Teixeira","family":"Lemes","sequence":"additional","affiliation":[{"name":"Universidade Federal de Alfenas , , ,","place":["Alfenas, Brasil, 37130-000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Clarissa","family":"dos Santos Pires","sequence":"additional","affiliation":[{"name":"Universidade Federal de Vic\u0327osa , , ,","place":["Vic\u0327osa, Brasil, 36570-000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8262-1091","authenticated-orcid":true,"given":"Luis Henrique Mendes","family":"da Silva","sequence":"additional","affiliation":[{"name":"Universidade Federal de Vic\u0327osa , , ,","place":["Vic\u0327osa, Brasil, 36570-000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9221-0421","authenticated-orcid":true,"given":"Yara Luiza","family":"Coelho Zampier","sequence":"additional","affiliation":[{"name":"Universidade Federal de Alfenas , , ,","place":["Alfenas, Brasil, 37130-000"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,7]]},"reference":[{"key":"2026092210563956100_cit1","doi-asserted-by":"publisher","first-page":"9673","DOI":"10.1021\/la301104a","article-title":"Impact of the Nanoparticle\u2013Protein\nCorona on Colloidal Stability and Protein Structure","volume":"28","author":"Gebauer","year":"2012","journal-title":"Langmuir"},{"key":"2026092210563956100_cit2","doi-asserted-by":"publisher","DOI":"10.3390\/nano14100823","article-title":"A Review for Uncovering the \u201cProtein-Nanoparticle\nAlliance\u201d: Implications of the Protein Corona for Biomedical\nApplications","volume":"14","author":"O\u0308nal Acet","year":"2024","journal-title":"Nanomaterials"},{"key":"2026092210563956100_cit3","doi-asserted-by":"publisher","first-page":"757","DOI":"10.1021\/cbe.4c00105","article-title":"Protein Corona\nof Nanoparticles: Isolation and Analysis","volume":"1","author":"Sun","year":"2024","journal-title":"Chem.\nBio Eng."},{"key":"2026092210563956100_cit4","doi-asserted-by":"publisher","first-page":"3141","DOI":"10.1038\/s41596-021-00546-w","article-title":"RNA-extraction-free\nnano-amplified colorimetric test for point-of-care clinical diagnosis\nof COVID-19","volume":"16","author":"Alafeef","year":"2021","journal-title":"Nat. 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