{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T12:13:18Z","timestamp":1788437598903,"version":"build-2803163510"},"reference-count":87,"publisher":"American Chemical Society (ACS)","issue":"34","license":[{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010661","name":"Horizon 2020 Framework Programme","doi-asserted-by":"publisher","award":["101131087"],"award-info":[{"award-number":["101131087"]}],"id":[{"id":"10.13039\/100010661","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003407","name":"Ministero dell?Istruzione, dell?Universit? e della Ricerca","doi-asserted-by":"publisher","award":["P2022FA79R"],"award-info":[{"award-number":["P2022FA79R"]}],"id":[{"id":"10.13039\/501100003407","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/na","name":"Red Espa?ola de Supercomputacion","doi-asserted-by":"publisher","award":["QHS-2025-2-0025"],"award-info":[{"award-number":["QHS-2025-2-0025"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Anhydrobiosis, the ability of organisms to survive extreme conditions such as desiccation, may represent a sustainable alternative to liquid-nitrogen (liquid nitrogen)-based cryopreservation once it can be induced in mammalian cells and germplasm. While natural xeroprotectants (e.g., signature peptides of Late Embryogenesis Abundant proteins and tardigrade disordered proteins, and vitamin E) can stabilize cells under drying, their use is limited to the laboratory level due to the high cost of extraction and the incomplete understanding of the molecular mechanisms. Here we employed a multiscale modeling framework spanning quantum mechanics (QM) and molecular dynamics to investigate how these representative xeroprotectants interact with mammalian model membranes (POPC\/DMPC) under desiccation and thermal cycling. We found that peptide-based xeroprotectants remain near headgroups and form dehydration-enhanced hydrogen bonds, whereas vitamin E partitions into the acyl chain region. This is consistent with suppressed pore formation in oxidized membranes. Across dry states, xeroprotectants generally increase acyl chain order and membrane rigidity, attenuate area shrinkage, and preserve bilayer thickness homogeneity. Notably, at higher peptide concentrations, chain overordering is mitigated, suggesting a tunable window for formulation. These results provide physicochemical guidelines for designing cost-effective, eco-friendly, and energy-efficient formulations of xeroprotectants and support the rational development of room-temperature preservation strategies with improved translational potential.<\/jats:p>","DOI":"10.1021\/acsomega.6c04138","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T10:01:45Z","timestamp":1786960905000},"page":"51254-51271","source":"Crossref","is-referenced-by-count":0,"title":["Screening of Xeroprotectants for Efficient Mammalian\nCell Structural Preservation in Dry Conditions"],"prefix":"10.1021","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6234-2749","authenticated-orcid":true,"given":"Valentin","family":"Diez-Cabanes","sequence":"first","affiliation":[{"name":"University of Burgos , , ,","place":["Burgos, Spain, 09001"]},{"name":"University of Burgos , , ,","place":["Burgos, Spain, 09001"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takahiro","family":"Kikawada","sequence":"additional","affiliation":[{"name":"The University of Tokyo , , , ,","place":["Kashiwa, Chiba, Japan, 277-8562"]},{"name":"National Agriculture and Food Research Organization , , Tsukuba , ,","place":["Ibaraki, Japan, 305-8634"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pasqualino","family":"Loi","sequence":"additional","affiliation":[{"name":"University of Teramo , , ,","place":["Teramo, Italy, 64100"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9996-2426","authenticated-orcid":true,"given":"Santiago","family":"Aparicio","sequence":"additional","affiliation":[{"name":"University of Burgos , , ,","place":["Burgos, Spain, 09001"]},{"name":"University of Burgos , , ,","place":["Burgos, Spain, 09001"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,17]]},"reference":[{"key":"2026090307184891400_cit1","doi-asserted-by":"publisher","first-page":"0963689721999617","DOI":"10.1177\/0963689721999617","article-title":"Cryopreservation: An Overview of Principles and Cell-Specific\nConsiderations","volume":"30","author":"Whaley","year":"2021","journal-title":"Cell Transplant"},{"key":"2026090307184891400_cit2","first-page":"375","article-title":"Cryoprotectants:\nThe Essential Antifreezes to Protect Life in the Frozen State","volume":"25","author":"Fuller","year":"2004","journal-title":"Cryo-Letters"},{"key":"2026090307184891400_cit3","doi-asserted-by":"publisher","first-page":"2200285","DOI":"10.1002\/adbi.202200285","article-title":"Cryopreservation: A Comprehensive Overview, Challenges, and Future\nPerspectives","volume":"7","author":"Parihar","year":"2023","journal-title":"Adv. Biol."},{"key":"2026090307184891400_cit4","unstructured":"https:\/\/www.rutherfordtitan.com\/liquid-nitrogen-generators\/liquid-nitrogen-price-usa\/ https:\/\/liquidnitrogengeneratorsus.com\/liquid-nitrogen-pricing (accessed 2025-09-10)."},{"key":"2026090307184891400_cit5","doi-asserted-by":"publisher","first-page":"1394","DOI":"10.1038\/1831394a0","article-title":"Prevention\nof Freezing Damage to Living Cells by Dimethyl\nSulfoxide","volume":"183","author":"Lovelock","year":"1959","journal-title":"Nature"},{"key":"2026090307184891400_cit6","doi-asserted-by":"publisher","first-page":"28","DOI":"10.1016\/0006-3002(53)90005-5","article-title":"Het Mechanism\nof the Protective Action of Glycerol against Haemolysis by Freezing\nand Thawing","volume":"11","author":"Lovelock","year":"1953","journal-title":"BBA - Biochim. Biophys. Acta"},{"key":"2026090307184891400_cit7","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1002\/j.1939-4640.1990.tb01583.x","article-title":"Cryopreservation\nof Mammalian Sperm:\nWhat We Ask Them to Survive","volume":"11","author":"Hammerstedt","year":"1990","journal-title":"J. Androl."},{"key":"2026090307184891400_cit8","unstructured":"https:\/\/apps.carboncloud.com\/climatehub\/product-reports\/id\/42847031980 (accessed 2025-09-10)."},{"key":"2026090307184891400_cit9","doi-asserted-by":"publisher","DOI":"10.1002\/btpr.3504","article-title":"Optimizing\nCryopreservation Strategies\nfor Scalable Cell Therapies: A Comprehensive Review with Insights\nfrom IPSC-Derived Therapies","volume":"40","author":"Dobruskin","year":"2024","journal-title":"Biotechnol. Prog."},{"key":"2026090307184891400_cit10","doi-asserted-by":"publisher","first-page":"2980","DOI":"10.1039\/D4MD00174E","article-title":"Trehalose in Cryopreservation. Applications,\nMechanisms and Intracellular Delivery Opportunities","volume":"15","author":"Murray","year":"2024","journal-title":"RSC Med. Chem."},{"key":"2026090307184891400_cit11","doi-asserted-by":"publisher","first-page":"579","DOI":"10.1038\/s41570-022-00407-4","article-title":"Chemical\nApproaches to Cryopreservation","volume":"6","author":"Murray","year":"2022","journal-title":"Nat. Rev. Chem."},{"key":"2026090307184891400_cit12","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1038\/72608","article-title":"Intracellular\nTrehalose Improves\nthe Survival of Cryopreserved Mammalian Cells","volume":"18","author":"Eroglu","year":"2000","journal-title":"Nat. Biotechnol."},{"key":"2026090307184891400_cit13","doi-asserted-by":"publisher","first-page":"3592","DOI":"10.1128\/aem.61.10.3592-3597.1995","article-title":"Trehalose\nand Sucrose Protect Both Membranes and Proteins in Intact Bacteria\nduring Drying","volume":"61","author":"Leslie","year":"1995","journal-title":"Appl. Environ. Microbiol."},{"key":"2026090307184891400_cit14","doi-asserted-by":"publisher","first-page":"519","DOI":"10.2337\/diab.46.3.519","article-title":"Trehalose: A Cryoprotectant\nThat Enhances Recovery and Preserves\nFunction of Human Pancreatic Islets after Long-Term Storage","volume":"46","author":"Beattie","year":"1997","journal-title":"Diabetes"},{"key":"2026090307184891400_cit15","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1159\/000499453","article-title":"New Approaches to\nCryopreservation of Cells, Tissues, and Organs","volume":"46","author":"Taylor","year":"2019","journal-title":"Transfus. Med. Hemotherapy"},{"key":"2026090307184891400_cit16","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1111\/jzo.12677","article-title":"Staying Young and Fit? Ontogenetic\nand Phylogenetic Consequences of Animal Anhydrobiosis","volume":"309","author":"Kaczmarek","year":"2019","journal-title":"J. Zool."},{"key":"2026090307184891400_cit17","doi-asserted-by":"publisher","first-page":"557","DOI":"10.3390\/insects13060557","article-title":"Deciphering the Biological\nEnigma-Genomic Evolution\nUnderlying Anhydrobiosis in the Phylum Tardigrada and the Chironomid\nPolypedilum Vanderplanki","volume":"13","author":"Yoshida","year":"2022","journal-title":"Insects"},{"key":"2026090307184891400_cit18","doi-asserted-by":"publisher","first-page":"4338","DOI":"10.1093\/jxb\/erac172","article-title":"Desiccation-Rehydration\nMeasurements in Bryophytes: Current Status and Future Insights","volume":"73","author":"Morales-Sa\u0301nchez","year":"2022","journal-title":"J. Exp. Bot."},{"key":"2026090307184891400_cit19","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1042\/bj20041931","article-title":"LEA Proteins\nPrevent Protein Aggregation\nDue to Water Stress","volume":"388","author":"Goyal","year":"2005","journal-title":"Biochem. J."},{"key":"2026090307184891400_cit20","doi-asserted-by":"publisher","first-page":"18073","DOI":"10.1073\/pnas.0706964104","article-title":"Hydrophilic Protein Associated with Desiccation Tolerance\nExhibits Broad Protein Stabilization Function","volume":"104","author":"Chakrabortee","year":"2007","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"2026090307184891400_cit21","doi-asserted-by":"publisher","first-page":"3440","DOI":"10.4236\/ajps.2014.522360","article-title":"Insights into Late Embryogenesis\nAbundant (LEA) Proteins in Plants: From Structure to the Functions","volume":"05","author":"Amara","year":"2014","journal-title":"Am. J. Plant Sci."},{"key":"2026090307184891400_cit22","doi-asserted-by":"publisher","first-page":"20859","DOI":"10.1073\/pnas.1214893109","article-title":"Late Embryogenesis\nAbundant Proteins Protect Human\nHepatoma Cells during Acute Desiccation","volume":"109","author":"Li","year":"2012","journal-title":"Proc.\nNatl. Acad. Sci. U. S. A."},{"key":"2026090307184891400_cit23","doi-asserted-by":"publisher","first-page":"409","DOI":"10.1111\/j.1748-1716.2011.02252.x","article-title":"Survival in Extreme Environments\n- on the Current Knowledge of Adaptations in Tardigrades","volume":"202","author":"M\u00f8bjerg","year":"2011","journal-title":"Acta Physiol."},{"key":"2026090307184891400_cit24","doi-asserted-by":"publisher","first-page":"178","DOI":"10.1186\/s12964-020-00670-2","article-title":"The Biology of Tardigrade\nDisordered Proteins in Extreme\nStress Tolerance","volume":"18","author":"Hesgrove","year":"2020","journal-title":"Cell Commun. Signal."},{"key":"2026090307184891400_cit25","doi-asserted-by":"publisher","first-page":"114063","DOI":"10.1016\/j.biopha.2022.114063","article-title":"The\nBiomedical Potential of Tardigrade\nProteins: A Review","volume":"158","author":"Kasianchuk","year":"2023","journal-title":"Biomed. Pharmacother."},{"key":"2026090307184891400_cit26","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3001780","article-title":"Stress-Dependent Cell\nStiffening by Tardigrade Tolerance Proteins That Reversibly Form a\nFilamentous Network and Gel","volume":"20","author":"Tanaka","year":"2022","journal-title":"PLoS Biol."},{"key":"2026090307184891400_cit27","doi-asserted-by":"publisher","first-page":"1050S","DOI":"10.1093\/ajcn\/53.4.1050S","article-title":"Protective\nRole of Vitamin E in Biological Systems","volume":"53","author":"Packer","year":"1991","journal-title":"Am.\nJ. Clin. Nutr."},{"key":"2026090307184891400_cit28","doi-asserted-by":"publisher","first-page":"3687","DOI":"10.1016\/j.febslet.2008.10.002","article-title":"Effects\nof \u03b1-Tocopherol (Vitamin E) on the Stability and Lipid Dynamics\nof Model Membranes Mimicking the Lipid Composition of Plant Chloroplast\nMembranes","volume":"582","author":"Hincha","year":"2008","journal-title":"FEBS Lett."},{"key":"2026090307184891400_cit29","doi-asserted-by":"publisher","first-page":"113536","DOI":"10.1016\/j.colsurfb.2023.113536","article-title":"The Effect of \u03b1-Tocopherol Incorporated into\nDifferent Carriers on the Oxidative Stability of Oil in Water (O\/W)\nEmulsions","volume":"230","author":"Olbin\u0301ska","year":"2023","journal-title":"Colloids Surfaces B Biointerfaces"},{"key":"2026090307184891400_cit30","doi-asserted-by":"publisher","first-page":"469","DOI":"10.1016\/0006-291X(88)90544-X","article-title":"Membrane Stabilization\nof Vitamin\nE: Interactions of \u0391-tocopherol with Phospholipids in Bilayer\nLiposomes","volume":"150","author":"Urano","year":"1988","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2026090307184891400_cit31","doi-asserted-by":"publisher","DOI":"10.15761\/ams.1000131","article-title":"Antioxidant Modified\nAmphiphilic Polymer Improves Intracellular Cryoprotectant Delivery\nand Alleviates Oxidative Stress in HeLa Cells","volume":"2","author":"Chen","year":"2017","journal-title":"Adv. Mater. Sci."},{"key":"2026090307184891400_cit32","first-page":"237","article-title":"Effects\nof Temperature on Cell Membranes","volume":"42","author":"Quinn","year":"1988","journal-title":"Symp. Soc.\nExp. Biol."},{"key":"2026090307184891400_cit33","doi-asserted-by":"publisher","first-page":"499","DOI":"10.1146\/annurev-biophys-112221-074832","article-title":"The Effects of Temperature\non Cellular Physiology","volume":"51","author":"Knapp","year":"2022","journal-title":"Annu. Rev. Biophys."},{"key":"2026090307184891400_cit34","doi-asserted-by":"publisher","first-page":"5392","DOI":"10.1073\/pnas.0408249102","article-title":"Molecular\nStructure of the Lecithin Ripple Phase","volume":"102","author":"De Vries","year":"2005","journal-title":"Proc.\nNatl. Acad. Sci. U. S. A."},{"key":"2026090307184891400_cit35","doi-asserted-by":"publisher","first-page":"045101","DOI":"10.1063\/1.2955753","article-title":"Solvent and Lipid Dynamics\nof Hydrated Lipid Bilayers by Incoherent Quasielastic Neutron Scattering","volume":"129","author":"Swenson","year":"2008","journal-title":"J. Chem. Phys."},{"key":"2026090307184891400_cit36","doi-asserted-by":"publisher","first-page":"1489","DOI":"10.1016\/j.bbamem.2018.04.014","article-title":"Investigation of\nPhase Transitions of Saturated Phosphocholine\nLipid Bilayers via Molecular Dynamics Simulations","volume":"1860","author":"Khakbaz","year":"2018","journal-title":"Biochim. Biophys. Acta-Biomembr."},{"key":"2026090307184891400_cit37","doi-asserted-by":"publisher","first-page":"14326","DOI":"10.1021\/jp0614796","article-title":"A Molecular Dynamics Investigation\nof the Influence\nof Hydration and Temperature on Structural and Dynamical Properties\nof a Dimyristoylphosphatidylcholine Bilayer","volume":"110","author":"Ho\u0308gberg","year":"2006","journal-title":"J. Phys. Chem. B"},{"key":"2026090307184891400_cit38","doi-asserted-by":"publisher","first-page":"3324","DOI":"10.1016\/S0006-3495(02)75333-2","article-title":"Structure of Gel Phase DMPC Determined\nby X-Ray Diffraction","volume":"83","author":"Tristram-Nagle","year":"2002","journal-title":"Biophys. J."},{"key":"2026090307184891400_cit39","doi-asserted-by":"publisher","first-page":"4023","DOI":"10.1016\/S0006-3495(03)74816-4","article-title":"Hydration, Structure,\nand Molecular Interactions in\nthe Headgroup Region of Dioleoylphosphatidylcholine Bilayers: An Electron\nSpin Resonance Study","volume":"85","author":"Ge","year":"2003","journal-title":"Biophys. J."},{"key":"2026090307184891400_cit40","doi-asserted-by":"publisher","first-page":"174904","DOI":"10.1063\/5.0044614","article-title":"Dehydration Induced\nDynamical Heterogeneity and Ordering\nMechanism of Lipid Bilayers","volume":"154","author":"Malik","year":"2021","journal-title":"J. Chem. Phys."},{"key":"2026090307184891400_cit41","doi-asserted-by":"publisher","first-page":"4515","DOI":"10.1021\/acs.jpclett.4c00332","article-title":"Dehydration of Lipid Membranes Drives\nRedistribution of Cholesterol Between Lateral Domains","volume":"15","author":"Orlikowska-Rzeznik","year":"2024","journal-title":"J. Phys. Chem. Lett."},{"key":"2026090307184891400_cit42","doi-asserted-by":"publisher","first-page":"196","DOI":"10.2142\/biophysico.16.0_196","article-title":"Replica Exchange\nMolecular Dynamics Simulation Study on the Mechanism of Desiccation-Induced\nStructuralization of an Intrinsically Disordered Peptide as a Model\nof LEA Proteins","volume":"16","author":"Nishimoto","year":"2019","journal-title":"Biophys. physicobiology"},{"key":"2026090307184891400_cit43","doi-asserted-by":"publisher","first-page":"5699","DOI":"10.1039\/C6CP08051K","article-title":"Alpha-Tocopherol Inhibits Pore Formation\nin Oxidized Bilayers","volume":"19","author":"Boonnoy","year":"2017","journal-title":"Phys. Chem. Chem. Phys."},{"key":"2026090307184891400_cit44","doi-asserted-by":"publisher","first-page":"5346","DOI":"10.1021\/acs.jpcb.1c02575","article-title":"Effects of Trehalose on Lipid Membranes\nunder Rapid Cooling Using All-Atom and Coarse-Grained Molecular Simulations","volume":"125","author":"Kozuch","year":"2021","journal-title":"J. Phys. Chem. B"},{"key":"2026090307184891400_cit45","doi-asserted-by":"publisher","first-page":"104898","DOI":"10.1016\/j.cryobiol.2024.104898","article-title":"Molecular Simulation\non the Interaction between Trehalose and Asymmetric Lipid Bilayer\nMimicking the Membrane of Human Red Blood Cells","volume":"115","author":"Cao","year":"2024","journal-title":"Cryobiology"},{"key":"2026090307184891400_cit46","doi-asserted-by":"publisher","first-page":"10732","DOI":"10.1021\/jp8025489","article-title":"Molecular Dynamics\nStudy on the Stabilization of Dehydrated\nLipid Bilayers with Glucose and Trehalose","volume":"112","author":"Leekumjorn","year":"2008","journal-title":"J.\nPhys. Chem. B"},{"key":"2026090307184891400_cit47","doi-asserted-by":"publisher","first-page":"490","DOI":"10.1016\/j.bpj.2009.05.007","article-title":"Water Replacement Hypothesis\nin Atomic Detail - Factors Determining the Structure of Dehydrated\nBilayer Stacks","volume":"97","author":"Golovina","year":"2009","journal-title":"Biophys. J."},{"key":"2026090307184891400_cit48","doi-asserted-by":"publisher","first-page":"2481","DOI":"10.1016\/j.bpj.2016.10.033","article-title":"Effects of Dimethyl Sulfoxide on\nSurface Water near Phospholipid Bilayers","volume":"111","author":"Lee","year":"2016","journal-title":"Biophys.\nJ."},{"key":"2026090307184891400_cit49","unstructured":"Rozas, S.; Righetti, G. I. C.; Diez-Cabanes, V.; De-la-Huerta-Sainz, S.; Ferna\u0301ndez-Pampi\u0301n, N.; Carlos Rumbo; ; Pietro, M. E. Di; Bol, A.; Mele, A.; Aparicio, S.\n          Dermal Safety of Deep Eutectic Solvents:\nA Multiscale Investigation of HMF-Levulinic Acid Skin Interactions.  Sustain. Chem. Pharm.2025. under\nrevision."},{"key":"2026090307184891400_cit50","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1007\/s00232-005-7006-8","article-title":"Structure of Fully Hydrated Fluid\nPhase Lipid Bilayers with Monounsaturated Chains","volume":"208","author":"Kuc\u030cerka","year":"2006","journal-title":"J. Membr. Biol."},{"key":"2026090307184891400_cit51","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1016\/S0304-4157(00)00016-2","article-title":"Structure\nof Lipid Bilayers","volume":"1469","author":"Nagle","year":"2000","journal-title":"Biochim. Biophys. Acta, Rev.\nBiomembr."},{"key":"2026090307184891400_cit52","doi-asserted-by":"publisher","first-page":"2761","DOI":"10.1016\/j.bbamem.2011.07.022","article-title":"Fluid Phase\nLipid Areas and Bilayer\nThicknesses of Commonly Used Phosphatidylcholines as a Function of\nTemperature","volume":"1808","author":"Kuc\u030cerka","year":"2011","journal-title":"Biochim. Biophys. Acta - Biomembr."},{"key":"2026090307184891400_cit53","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1104\/pp.108.120725","article-title":"The Enigmatic\nLEA Proteins and Other Hydrophilins","volume":"148","author":"Battaglia","year":"2008","journal-title":"Plant Physiol."},{"key":"2026090307184891400_cit54","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1146\/annurev-physiol-012110-142203","article-title":"LEA Proteins\nDuring Water Stress: Not Just for Plants Anymore","volume":"73","author":"Hand","year":"2011","journal-title":"Annu. Rev. Physiol."},{"key":"2026090307184891400_cit55","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0044209","article-title":"Two Novel\nHeat-Soluble Protein Families Abundantly Expressed in an Anhydrobiotic\nTardigrade","volume":"7","author":"Yamaguchi","year":"2012","journal-title":"PLoS One"},{"key":"2026090307184891400_cit56","doi-asserted-by":"publisher","first-page":"1093","DOI":"10.1021\/bi901745f","article-title":"Desiccation-Induced\nStructuralization and Glass Formation of Group 3 Late Embryogenesis\nAbundant Protein Model Peptides","volume":"49","author":"Shimizu","year":"2010","journal-title":"Biochemistry"},{"key":"2026090307184891400_cit57","doi-asserted-by":"publisher","first-page":"51","DOI":"10.20585\/cryobolcryotechnol.59.1_51","article-title":"Structural and Functional Studies on Model Peptides\nfor Group-3 Late Embryogenesis Abundant Proteins (Papers presented\nat the Seminar,\" NIAS International Seminar for Cryobiology and\nCryotechnology\"","volume":"59","author":"Furuki","year":"2013","journal-title":"Cryobiol. Cryotechnol."},{"key":"2026090307184891400_cit58","doi-asserted-by":"publisher","first-page":"891","DOI":"10.1016\/j.bbapap.2012.04.013","article-title":"Effects of Group 3 LEA Protein Model\nPeptides on Desiccation-Induced Protein Aggregation","volume":"1824","author":"Furuki","year":"2012","journal-title":"Biochim. Biophys. Acta - Proteins Proteomics"},{"key":"2026090307184891400_cit59","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5135","article-title":"Structural\nAdaptability and Surface Activity of Peptides Derived from Tardigrade\nProteins","volume":"33","author":"Giubertoni","year":"2024","journal-title":"Protein Sci."},{"key":"2026090307184891400_cit60","doi-asserted-by":"publisher","first-page":"400","DOI":"10.1016\/j.mam.2007.05.004","article-title":"Vitamin\nE: An Overview of Major Research Directions","volume":"28","author":"Zingg","year":"2007","journal-title":"Mol. Aspects Med."},{"key":"2026090307184891400_cit61","doi-asserted-by":"publisher","first-page":"8351","DOI":"10.1021\/acs.jpcb.8b05016","article-title":"Vitamin\ne Has Reduced Affinity for a Polyunsaturated\nPhospholipid: An Umbrella Sampling Molecular Dynamics Simulations\nStudy","volume":"122","author":"Leng","year":"2018","journal-title":"J. Phys. Chem. B"},{"key":"2026090307184891400_cit62","doi-asserted-by":"publisher","DOI":"10.1002\/pro.4792","article-title":"UCSF ChimeraX:\nTools for Structure\nBuilding and Analysis","volume":"32","author":"Meng","year":"2023","journal-title":"Protein Sci."},{"key":"2026090307184891400_cit63","doi-asserted-by":"publisher","first-page":"3865","DOI":"10.1103\/PhysRevLett.77.3865","article-title":"Generalized Gradient Approximation\nMade Simple","volume":"77","author":"Perdew","year":"1996","journal-title":"Phys. Rev. Lett."},{"key":"2026090307184891400_cit64","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1002\/wcms.1159","article-title":"Cp2k: Atomistic\nSimulations of Condensed Matter Systems","volume":"4","author":"Hutter","year":"2014","journal-title":"Wiley\nInterdiscip. Rev. Comput. Mol. Sci."},{"key":"2026090307184891400_cit65","doi-asserted-by":"publisher","first-page":"1703","DOI":"10.1103\/physrevb.54.1703","article-title":"Separable Dual-Space Gaussian Pseudopotentials","volume":"54","author":"Goedecker","year":"1996","journal-title":"Phys. Rev. B"},{"key":"2026090307184891400_cit66","doi-asserted-by":"publisher","first-page":"2224","DOI":"10.1021\/j100007a062","article-title":"Conductor-like\nScreening Model for Real Solvents: A New Approach to the Quantitative\nCalculation of Solvation Phenomena","volume":"99","author":"Klamt","year":"1995","journal-title":"J. Phys.\nChem."},{"key":"2026090307184891400_cit67","unstructured":"Frisch, M. J.; Trucks, G. W.; Schlegel, H. B.; Scuseria, G. E.; Robb, M. A.; Cheeseman, J. R.; Scalmani, G.; Barone, V.; Petersson, G. a.; Nakatsuji, H.; Li, X.; Caricato, M.; Marenich, a. V.; Bloino, J.; Janesko, B. G.; Gomperts, R.; Mennucci, B.; Hratchian, H. P.; Ortiz, J. V.; Izmaylov, a. F.; Sonnenberg, J. L.; Williams; ; Ding, F.; Lipparini, F.; Egidi, F.; Goings, J.; Peng, B.; Petrone, A.; Henderson, T.; Ranasinghe, D.; Zakrzewski, V. G.; Gao, J.; Rega, N.; Zheng, G.; Liang, W.; Hada, M.; Ehara, M.; Toyota, K.; Fukuda, R.; Hasegawa, J.; Ishida, M.; Nakajima, T.; Honda, Y.; Kitao, O.; Nakai, H.; Vreven, T.; Throssell, K.; Montgomery, Jr. J. a.; Peralta, J. E.; Ogliaro, F.; Bearpark, M. J.; Heyd, J. J.; Br; et al., Gaussian 16, Revision C.01; Gaussian,\nInc.: Wallingford CT, 2016."},{"key":"2026090307184891400_cit68","doi-asserted-by":"publisher","first-page":"3343","DOI":"10.1021\/acs.jpcb.9b11728","article-title":"COSMO Perm:\nMechanistic Prediction of Passive Membrane Permeability\nfor Neutral Compounds and Ions and Its PH Dependence","volume":"124","author":"Schwo\u0308bel","year":"2020","journal-title":"J. Phys. Chem. B"},{"key":"2026090307184891400_cit69","doi-asserted-by":"publisher","first-page":"10573","DOI":"10.1039\/C5CP07379K","article-title":"Simulation of Lipid Bilayer Self-Assembly\nUsing All-Atom Lipid Force Fields","volume":"18","author":"Skjevik","year":"2016","journal-title":"Phys. Chem.\nChem. Phys."},{"key":"2026090307184891400_cit70","doi-asserted-by":"publisher","first-page":"1186","DOI":"10.3390\/ijms19041186","article-title":"Molecular\nDynamics Simulations of Human Antimicrobial Peptide LL-37 in Model\nPOPC and POPG Lipid Bilayers","volume":"19","author":"Zhao","year":"2018","journal-title":"Int. J. Mol. Sci."},{"key":"2026090307184891400_cit71","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1007\/s00894-019-3964-0","article-title":"Physical Properties\nof Model Biological Lipid Bilayers: Insights from All-Atom Molecular\nDynamics Simulations","volume":"25","author":"Shahane","year":"2019","journal-title":"J. Mol. Model."},{"key":"2026090307184891400_cit72","doi-asserted-by":"publisher","first-page":"012022","DOI":"10.1088\/1742-6596\/1136\/1\/012022","article-title":"Molecular Dynamics Study of Binary\nPOPC Bilayers: Molecular Condensing Effects on Membrane Structure\nand Dynamics","volume":"1136","author":"Saito","year":"2018","journal-title":"J. Phys. Conf. Ser."},{"key":"2026090307184891400_cit73","doi-asserted-by":"publisher","first-page":"1859","DOI":"10.1002\/jcc.20945","article-title":"CHARMM-GUI: A Web-Based Graphical\nUser Interface for CHARMM","volume":"29","author":"Jo","year":"2008","journal-title":"J. Comput. Chem."},{"key":"2026090307184891400_cit74","doi-asserted-by":"publisher","first-page":"405","DOI":"10.1021\/acs.jctc.5b00935","article-title":"CHARMM-GUI Input Generator\nfor NAMD, GROMACS, AMBER, OpenMM, and CHARMM\/OpenMM Simulations Using\nthe CHARMM36 Additive Force Field","volume":"12","author":"Lee","year":"2016","journal-title":"J. Chem.\nTheory Comput."},{"key":"2026090307184891400_cit75","doi-asserted-by":"publisher","first-page":"2135","DOI":"10.1002\/jcc.23354","article-title":"CHARMM36\nAll-Atom Additive Protein Force Field: Validation\nBased on Comparison to NMR Data","volume":"34","author":"Huang","year":"2013","journal-title":"J. Comput.\nChem."},{"key":"2026090307184891400_cit76","doi-asserted-by":"publisher","first-page":"2773","DOI":"10.1016\/j.bpj.2023.05.033","article-title":"Automated\nSimulation-Based Membrane Protein Refinement\ninto Cryo-EM Data","volume":"122","author":"Yvonnesdotter","year":"2023","journal-title":"Biophys. J."},{"key":"2026090307184891400_cit77","doi-asserted-by":"publisher","first-page":"014101","DOI":"10.1063\/1.2408420","article-title":"Canonical\nSampling through Velocity\nRescaling","volume":"126","author":"Bussi","year":"2007","journal-title":"J. Chem. Phys."},{"key":"2026090307184891400_cit78","doi-asserted-by":"publisher","first-page":"114107","DOI":"10.1063\/5.0020514","article-title":"Pressure Control Using\nStochastic Cell Rescaling","volume":"153","author":"Bernetti","year":"2020","journal-title":"J. Chem. Phys."},{"key":"2026090307184891400_cit79","doi-asserted-by":"publisher","first-page":"8577","DOI":"10.1063\/1.470117","article-title":"A Smooth Particle Mesh Ewald Method","volume":"103","author":"Essmann","year":"1995","journal-title":"J. Chem. Phys."},{"key":"2026090307184891400_cit80","doi-asserted-by":"publisher","first-page":"1952","DOI":"10.1002\/jcc.21172","article-title":"GridMAT-MD:\nA Grid-Based Membrane\nAnalysis Tool for Use With Molecular Dynamics","volume":"30","author":"Allen","year":"2009","journal-title":"J. Comput. Chem."},{"key":"2026090307184891400_cit81","doi-asserted-by":"publisher","first-page":"6184","DOI":"10.1021\/acs.chemrev.8b00460","article-title":"Computational\nModeling of Realistic\nCell Membranes","volume":"119","author":"Marrink","year":"2019","journal-title":"Chem. Rev."},{"key":"2026090307184891400_cit82","doi-asserted-by":"publisher","first-page":"5966","DOI":"10.33011\/livecoms.1.1.5966","article-title":"Simulation Best\nPractices for Lipid\nMembranes","volume":"1","author":"Smith","year":"2019","journal-title":"Living J. Comput. Mol. Sci."},{"key":"2026090307184891400_cit83","doi-asserted-by":"publisher","first-page":"4644","DOI":"10.1039\/C8RA08441F","article-title":"Shedding Light on the\nStructural\nProperties of Lipid Bilayers Using Molecular Dynamics Simulation:\nA Review Study","volume":"9","author":"Moradi","year":"2019","journal-title":"RSC Adv."},{"key":"2026090307184891400_cit84","doi-asserted-by":"publisher","first-page":"963","DOI":"10.1016\/j.tibs.2023.08.004","article-title":"Membrane Homeostasis beyond Fluidity: Control of Membrane Compressibility","volume":"48","author":"Renne","year":"2023","journal-title":"Trends Biochem. Sci."},{"key":"2026090307184891400_cit85","doi-asserted-by":"publisher","first-page":"246","DOI":"10.1016\/j.bbamem.2014.06.004","article-title":"Elastic Deformation\nand Area per Lipid of Membranes: Atomistic View from Solid-State Deuterium\nNMR Spectroscopy","volume":"1848","author":"Kinnun","year":"2015","journal-title":"Biochim. Biophys. Acta - Biomembr."},{"key":"2026090307184891400_cit86","doi-asserted-by":"publisher","first-page":"091103","DOI":"10.1063\/5.0137727","article-title":"Quantifying\nDynamical Heterogeneity\nLength Scales of Interface Water across Model Membrane Phase Transitions","volume":"158","author":"Malik","year":"2023","journal-title":"J. Chem. Phys."},{"key":"2026090307184891400_cit87","doi-asserted-by":"publisher","first-page":"5998","DOI":"10.1021\/acs.jpcb.5c02234","article-title":"What Are the\nEssential Water Molecules in Regulating\nLipid Membrane Fluidity?","volume":"129","author":"Kumar","year":"2025","journal-title":"J. Phys. Chem. B"}],"container-title":["ACS Omega"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsomega.6c04138","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/acsodf\/article-pdf\/11\/34\/51254\/66609025\/acsomega.6c04138.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/acsodf\/article-pdf\/11\/34\/51254\/66609025\/acsomega.6c04138.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T11:18:59Z","timestamp":1788434339000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/acsodf\/article\/11\/34\/51254\/5259583\/Screening-of-Xeroprotectants-for-Efficient"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,17]]},"references-count":87,"journal-issue":{"issue":"34","published-online":{"date-parts":[[2026,8,17]]},"published-print":{"date-parts":[[2026,9,1]]}},"URL":"https:\/\/doi.org\/10.1021\/acsomega.6c04138","relation":{},"ISSN":["2470-1343"],"issn-type":[{"value":"2470-1343","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,17]]}}}