{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T19:46:15Z","timestamp":1792784775306,"version":"4.5.0"},"reference-count":43,"publisher":"American Chemical Society (ACS)","issue":"38","license":[{"start":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T00:00:00Z","timestamp":1789344000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"Youth Elite Project of Anhui Academy of Agricultural Sciences","award":["QNYC 202511"],"award-info":[{"award-number":["QNYC 202511"]}],"id":[{"id":"https:\/\/ror.org\/01pw5qp76","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Colon cancer ranks among the most prevalent malignant tumors worldwide, with its incidence rising progressively year by year. The identification of novel research tool for therapeutic targets has thus become an urgent priority. Here, we constructed a CS\u2022CBE integrative delivery and editing system via chitosan and cytosine base editor (CBE), which could successfully target and inhibit the phosphorylation of \u03b2-catenin. In vitro studies demonstrated that this system achieved a high delivery efficiency of 86.0% and an editing efficiency of 78% in HCT116 cells, and Transwell and wound-healing assays verified that the CBE-\u03b2-catenin-HCT116 cells exhibited an increased proliferation rate. Furthermore, a xenograft tumor model was established in nude mice, and we observed that the tumor volume and weight in the experimental group were both significantly increased compared with those in the control group. Subsequently, the candidate genes identified from transcriptomic results, such as AXIN2 and GPR15, which are downstream of \u03b2-catenin pathways, validated the feasibility of the CS\u2022CBE system as a research tool. Collectively, our CS\u2022CBE system serves as a valuable research tool for targeted protein modulation, downstream target identification, and probe specific post-translational modifications analysis in colon cancer research.<\/jats:p>","DOI":"10.1021\/acsomega.6c04206","type":"journal-article","created":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T14:01:40Z","timestamp":1789394500000},"page":"57034-57045","source":"Crossref","is-referenced-by-count":0,"title":["A CS\u2022CBE\nSystem Targeting \u03b2-Catenin:\nExploring Additional Inhibition Targets for Colon Cancer Proliferation"],"prefix":"10.1021","volume":"11","author":[{"given":"Min","family":"Li","sequence":"first","affiliation":[{"name":"Anhui Academy of Agriculture Sciences , , ,","place":["Hefei, China, 230031"]},{"name":"Anhui University , , ,","place":["Hefei, China, 230601"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3520-1721","authenticated-orcid":true,"given":"Qian","family":"Ban","sequence":"additional","affiliation":[{"name":"Anhui University , , ,","place":["Hefei, China, 230601"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dahai","family":"Liu","sequence":"additional","affiliation":[{"name":"Foshan University , , , ,","place":["Foshan, Guangdong, PR China, 528231"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bingang","family":"Li","sequence":"additional","affiliation":[{"name":"Anhui University , , ,","place":["Hefei, China, 230601"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jintang","family":"Xu","sequence":"additional","affiliation":[{"name":"Anhui University , , ,","place":["Hefei, China, 230601"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaqi","family":"Geng","sequence":"additional","affiliation":[{"name":"Anhui University , , ,","place":["Hefei, China, 230601"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dakun","family":"Chen","sequence":"additional","affiliation":[{"name":"Anhui University , , ,","place":["Hefei, China, 230601"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1645-1311","authenticated-orcid":true,"given":"Wenqiao","family":"Hui","sequence":"additional","affiliation":[{"name":"Anhui Academy of Agriculture Sciences , , ,","place":["Hefei, China, 230031"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,14]]},"reference":[{"issue":"10449","key":"2026092911114171600_cit1","doi-asserted-by":"publisher","first-page":"294","DOI":"10.1016\/S0140-6736(24)00360-X","article-title":"Colorectal cancer","volume":"404","author":"Eng","year":"2024","journal-title":"The Lancet"},{"issue":"1","key":"2026092911114171600_cit2","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1007\/s12029-025-01363-0","article-title":"Comparative Outcomes\nof Colorectal Cancer Patients Undergoing Elective and Emergency Surgeries:\nA Propensity Score Matched Cohort Study","volume":"57","author":"Kedareswar","year":"2026","journal-title":"J.\nGastrointest. 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Oncol.\nHematol."},{"issue":"1","key":"2026092911114171600_cit4","doi-asserted-by":"publisher","first-page":"72","DOI":"10.3390\/cancers18010072","article-title":"PUMA-p53 Dysregulation\nand Ki-67 Overexpression Define Unfavorable Prognostic Signatures\nin Colorectal Cancer","volume":"18","author":"Mekras","year":"2026","journal-title":"Cancers"},{"issue":"4","key":"2026092911114171600_cit5","doi-asserted-by":"publisher","first-page":"308","DOI":"10.3760\/cma.j.cn112152-20240308-00102","article-title":"Comparison analyses\nof global burden of colorectal\ncancer","volume":"47","author":"Li","year":"2025","journal-title":"Zhonghua Zhong Liu Za Zhi"},{"issue":"9","key":"2026092911114171600_cit6","doi-asserted-by":"publisher","first-page":"998","DOI":"10.2174\/1389450121999201117115717","article-title":"Colorectal Cancer Epidemiology: Recent\nTrends and Impact on Outcomes","volume":"22","author":"Baidoun","year":"2021","journal-title":"Curr. 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Nanomed"},{"key":"2026092911114171600_cit36","doi-asserted-by":"publisher","first-page":"114221","DOI":"10.1016\/j.colsurfb.2024.114221","article-title":"Utilization of chitosan\nnanocomposites loaded with quantum dots enables efficient and traceable\nDNA delivery","volume":"245","author":"He","year":"2025","journal-title":"Colloids Surf., B"},{"issue":"4","key":"2026092911114171600_cit37","doi-asserted-by":"publisher","first-page":"1396","DOI":"10.1016\/j.ymthe.2022.01.040","article-title":"Evaluation of cytosine base editing and adenine base\nediting as a potential treatment for alpha-1 antitrypsin deficiency","volume":"30","author":"Packer","year":"2022","journal-title":"Mol. Ther."},{"issue":"17","key":"2026092911114171600_cit38","doi-asserted-by":"publisher","first-page":"6240","DOI":"10.3390\/ijms21176240","article-title":"CRISPR-Cas9 DNA Base-Editing and\nPrime-Editing","volume":"21","author":"Kantor","year":"2020","journal-title":"Int. J. Mol. 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