{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T20:08:50Z","timestamp":1793045330734,"version":"4.5.0"},"reference-count":44,"publisher":"American Chemical Society (ACS)","issue":"32","license":[{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001823","name":"Ministerstvo ?kolstv?, Ml?de?e a Telov?chovy","doi-asserted-by":"publisher","award":["CZ.02.01.01\/00\/22_008\/0004597"],"award-info":[{"award-number":["CZ.02.01.01\/00\/22_008\/0004597"]}],"id":[{"id":"10.13039\/501100001823","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001824","name":"Grantov? Agentura Cesk? Republiky","doi-asserted-by":"publisher","award":["23-07356S"],"award-info":[{"award-number":["23-07356S"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Allicin exhibits several therapeutic properties, but its clinical use is limited by its high instability and low bioavailability. Furthermore, although pulmonary delivery of allicin offers a promising approach for treating various respiratory diseases, direct exposure of the lungs to allicin through aerosols or vapors carries a significant risk of respiratory tract irritation or tissue damage. Facing these challenges, we propose a novel bioinspired strategy that facilitates the in situ synthesis of allicin from its stabilized precursors using the Bu\u0308chi Mini Spray Dryer equipped with a three-fluid (3F) nozzle to develop inhalable carriers suitable for deep lung delivery. We have identified conditions that ensure successful coencapsulation of both enzyme and substrate within maltodextrin core\u2013shell microparticles, and we have examined the effects of selected additives on allicin yield and aerosolization performance under various storage conditions. Each 3F particle acts as an independent enzyme\u2013substrate system that, upon deposition, dissolves and releases active allicin within the network of the respiratory tract. We believe that such microcarriers may help overcome the challenges of pulmonary delivery of allicin and other unstable compounds.<\/jats:p>","DOI":"10.1021\/acsomega.6c04308","type":"journal-article","created":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T12:02:33Z","timestamp":1785931353000},"page":"48144-48156","source":"Crossref","is-referenced-by-count":2,"title":["Inhalable Spray-Dried Carriers for Deep Lung Delivery\nand In Situ Synthesis of Allicin"],"prefix":"10.1021","volume":"11","author":[{"given":"Lucie","family":"Mas\u030ckova\u0301","sequence":"first","affiliation":[{"name":"University of Chemistry and Technology Prague , , Technicka\u0301 5 , ,","place":["Prague 6, Czech Republic, 166 28"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ma\u0301ria","family":"Kalina","sequence":"additional","affiliation":[{"name":"University of Chemistry and Technology Prague , , Technicka\u0301 5 , ,","place":["Prague 6, Czech Republic, 166 28"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karoli\u0301na","family":"Slonkova\u0301","sequence":"additional","affiliation":[{"name":"University of Chemistry and Technology Prague , , Technicka\u0301 5 , ,","place":["Prague 6, Czech Republic, 166 28"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lara","family":"Mugrauer","sequence":"additional","affiliation":[{"name":"University of Chemistry and Technology Prague , , Technicka\u0301 5 , ,","place":["Prague 6, Czech Republic, 166 28"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vojte\u030cch","family":"Klims\u030ca","sequence":"additional","affiliation":[{"name":"University of Chemistry and Technology Prague , , Technicka\u0301 5 , ,","place":["Prague 6, Czech Republic, 166 28"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Reiserova\u0301","sequence":"additional","affiliation":[{"name":"University of Chemistry and Technology Prague , , Technicka\u0301 5 , ,","place":["Prague 6, Czech Republic, 166 28"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kater\u030cina","family":"Slaninova\u0301","sequence":"additional","affiliation":[{"name":"University of Chemistry and Technology Prague , , Technicka\u0301 5 , ,","place":["Prague 6, Czech Republic, 166 28"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1478-9754","authenticated-orcid":true,"given":"Denisa","family":"Lizon\u030cova\u0301","sequence":"additional","affiliation":[{"name":"University of Chemistry and Technology Prague , , Technicka\u0301 5 , ,","place":["Prague 6, Czech Republic, 166 28"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8740-415X","authenticated-orcid":true,"given":"Viola","family":"Toka\u0301rova\u0301","sequence":"additional","affiliation":[{"name":"University of Chemistry and Technology Prague , , Technicka\u0301 5 , ,","place":["Prague 6, Czech Republic, 166 28"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5353-6069","authenticated-orcid":true,"given":"Ondr\u030cej","family":"Kas\u030cpar","sequence":"additional","affiliation":[{"name":"University of Chemistry and Technology Prague , , Technicka\u0301 5 , ,","place":["Prague 6, Czech Republic, 166 28"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,5]]},"reference":[{"key":"2026081811085693500_cit1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1039\/BK9780854041909-00001","article-title":"Allium botany\nand cultivation, ancient and modern","author":"Block","year":"2009","journal-title":"Garlic\nand other alliums: the lore and the science"},{"issue":"11","key":"2026081811085693500_cit2","doi-asserted-by":"publisher","first-page":"1950","DOI":"10.1021\/ja01239a048","article-title":"Allicin, the antibacterial principle\nof Allium sativum.\nI. Isolation, physical properties and antibacterial action","volume":"66","author":"Cavallito","year":"1944","journal-title":"J. Am. Chem. Soc."},{"issue":"3","key":"2026081811085693500_cit3","doi-asserted-by":"publisher","first-page":"114","DOI":"10.1007\/BF02137698","article-title":"U\u0308ber alliin,\ndie genuine muttersubstanz des\nKnoblaucho\u0308ls","volume":"3","author":"Stoll","year":"1947","journal-title":"Experientia"},{"issue":"3","key":"2026081811085693500_cit4","doi-asserted-by":"publisher","first-page":"716S","DOI":"10.1093\/jn\/136.3.716S","article-title":"Clarifying\nthe real bioactive constituents of garlic","volume":"136","author":"Amagase","year":"2006","journal-title":"J.\nNutr."},{"key":"2026081811085693500_cit5","doi-asserted-by":"publisher","first-page":"451","DOI":"10.1007\/s12223-020-00786-5","article-title":"Antimicrobial properties of allicin used alone or in combination\nwith other medications","volume":"65","author":"Choo","year":"2020","journal-title":"Folia Microbiol."},{"issue":"11","key":"2026081811085693500_cit6","doi-asserted-by":"publisher","first-page":"2877","DOI":"10.1271\/bbb.80381","article-title":"Thermostability of allicin\ndetermined by chemical and biological assays","volume":"72","author":"Fujisawa","year":"2008","journal-title":"Biosci., Biotechnol., Biochem."},{"key":"2026081811085693500_cit7","doi-asserted-by":"publisher","first-page":"502","DOI":"10.1016\/j.tifs.2019.03.003","article-title":"Allicin and health:\nA comprehensive review","volume":"86","author":"Salehi","year":"2019","journal-title":"Trends Food Sci. 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Bioeng."},{"issue":"10","key":"2026081811085693500_cit9","doi-asserted-by":"publisher","first-page":"1711","DOI":"10.3390\/molecules22101711","article-title":"Diallylthiosulfinate\n(Allicin), a volatile antimicrobial from garlic (Allium sativum),\nkills human lung pathogenic bacteria, including MDR strains, as a\nvapor","volume":"22","author":"Reiter","year":"2017","journal-title":"Molecules"},{"issue":"1","key":"2026081811085693500_cit10","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1021\/bp034135v","article-title":"Thermal degradation\nof allicin in garlic extracts and its implication on the inhibition\nof the in-vitro growth of Helicobacter pylori","volume":"20","author":"Can\u0303izares","year":"2008","journal-title":"Biotechnol. 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Aerosol Sci."},{"issue":"1","key":"2026081811085693500_cit33","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1016\/S0003-2697(02)00010-6","article-title":"A spectrophotometric\nassay for allicin, alliin, and alliinase (alliin lyase) with a chromogenic\nthiol: reaction of 4-mercaptopyridine with thiosulfinates","volume":"307","author":"Miron","year":"2002","journal-title":"Anal. Biochem."},{"issue":"11","key":"2026081811085693500_cit34","first-page":"599","article-title":"Separation\nand purification of alliinase\nand alliin from garlic (Allium sativum)","volume":"2","author":"Mallika","year":"2014","journal-title":"J.\nAcad. Ind. 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