{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T16:58:07Z","timestamp":1790701087653,"version":"4.1.0"},"reference-count":34,"publisher":"American Chemical Society (ACS)","issue":"38","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100018703","name":"HORIZON EUROPE European Innovation Council","doi-asserted-by":"publisher","award":["101047263"],"award-info":[{"award-number":["101047263"]}],"id":[{"id":"10.13039\/100018703","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011773","name":"Conseil r?gional de Bourgogne-Franche-Comt?","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011773","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>In vivo fluorescence lifetime imaging (FLI) is an advanced optical imaging modality with the capability to reveal additional biological information compared to fluorescence intensity (FI) imaging. In addition to enabling tissue discrimination based on microenvironmental variations, lifetime contrast allows the differentiation of spectrally overlapping fluorophores, thereby expanding the high potential of multiplexing imaging. Until now, most of the studies evaluating the benefits of in vivo FLI in terms of enhanced sensitivity and specificity for tumor imaging with fluorescent contrast agents have been conducted using heptamethine cyanine dyes. While these fluorophores are highly successful for conventional in vivo fluorescence imaging, they are characterized by short singlet excited-state lifetimes (generally below 1 ns), making it challenging to achieve satisfying tissue contrast and to implement multiplexing imaging strategies based on their lifetime differences. Here, we report a bioconjugatable, water-soluble NIR-emissive aza-BODIPY dye with a 100% longer lifetime than IRDye 800CW in physiological conditions. We demonstrate its suitability for in vivo FLI when conjugated to cetuximab and reveal how conjugation methods impact the lifetime characteristics of fluorescent antibody conjugates.<\/jats:p>","DOI":"10.1021\/acsomega.6c04430","type":"journal-article","created":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T01:01:48Z","timestamp":1789520508000},"page":"57061-57071","source":"Crossref","is-referenced-by-count":0,"title":["Near-Infrared Organic\nStructures with Extended Lifetimes\nto Unlock the Potential of\n                    <i>In Vivo<\/i>\n                    Fluorescence Lifetime\nImaging"],"prefix":"10.1021","volume":"11","author":[{"given":"Elisa","family":"Chazeau","sequence":"first","affiliation":[{"name":"Universite\u0301 Bourgogne Europe, CNRS, ICMUB UMR 6302 , 9 Avenue Alain Savary , ,","place":["Dijon, France, 21000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hassan Al","family":"Sabea","sequence":"additional","affiliation":[{"name":"Universite\u0301 Bourgogne Europe, CNRS, ICMUB UMR 6302 , 9 Avenue Alain Savary , ,","place":["Dijon, France, 21000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone","family":"Janssen","sequence":"additional","affiliation":[{"name":"Vrije Universiteit Brussel (VUB) , , Laarbeeklaan 103 , ,","place":["Brussels, Belgium, 1090"]},{"name":"Universiteit Gent , , Salisburylaan 133 , ,","place":["Merelbeke, Belgium, 9820"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2837-8342","authenticated-orcid":true,"given":"\u0141ukasz","family":"Mateusiak","sequence":"additional","affiliation":[{"name":"Vrije Universiteit Brussel (VUB) , , Laarbeeklaan 103 , ,","place":["Brussels, Belgium, 1090"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Van den Dries","sequence":"additional","affiliation":[{"name":"Vrije Universiteit Brussel (VUB) , , Pleinlaan 2 , ,","place":["Elsene, Belgium, 1050"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Lapauw","sequence":"additional","affiliation":[{"name":"Vrije Universiteit Brussel (VUB) , , Pleinlaan 2 , ,","place":["Elsene, Belgium, 1050"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sevada","family":"Sahakian","sequence":"additional","affiliation":[{"name":"Vrije Universiteit Brussel (VUB) , , Pleinlaan 2 , ,","place":["Elsene, Belgium, 1050"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7331-7135","authenticated-orcid":true,"given":"Marcus C.M.","family":"Stroet","sequence":"additional","affiliation":[{"name":"Vrije Universiteit Brussel (VUB) , , Laarbeeklaan 103 , ,","place":["Brussels, Belgium, 1090"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6883-2529","authenticated-orcid":true,"given":"Tim","family":"Dreessen","sequence":"additional","affiliation":[{"name":"Vrije Universiteit Brussel (VUB) , , Laarbeeklaan 103 , ,","place":["Brussels, Belgium, 1090"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maarten","family":"Kuijk","sequence":"additional","affiliation":[{"name":"Vrije Universiteit Brussel (VUB) , , Pleinlaan 2 , ,","place":["Elsene, Belgium, 1050"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7657-5535","authenticated-orcid":true,"given":"Catherine","family":"Paul","sequence":"additional","affiliation":[{"name":"EPHE, PSL Research University , ,","place":["Paris, France, 75000"]},{"name":"Universite\u0301 Bourgogne Europe, INSERM, CTM UMR 1231 , ,","place":["Dijon, France, 21000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans","family":"Ingelberts","sequence":"additional","affiliation":[{"name":"Vrije Universiteit Brussel (VUB) , , Pleinlaan 2 , ,","place":["Elsene, Belgium, 1050"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8739-6137","authenticated-orcid":true,"given":"Sophie","family":"Hernot","sequence":"additional","affiliation":[{"name":"Vrije Universiteit Brussel (VUB) , , Laarbeeklaan 103 , ,","place":["Brussels, Belgium, 1090"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2300-4499","authenticated-orcid":true,"given":"Anthony","family":"Romieu","sequence":"additional","affiliation":[{"name":"Universite\u0301 Bourgogne Europe, CNRS, ICMUB UMR 6302 , 9 Avenue Alain Savary , ,","place":["Dijon, France, 21000"]},{"name":"Universite\u0301 Co\u0302te d\u2019Azur, CNRS , , 28 Avenue Valrose , ,","place":["Nice, France, 06100"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3484-3837","authenticated-orcid":true,"given":"Christine","family":"Goze","sequence":"additional","affiliation":[{"name":"Universite\u0301 Bourgogne Europe, CNRS, ICMUB UMR 6302 , 9 Avenue Alain Savary , ,","place":["Dijon, France, 21000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franck","family":"Denat","sequence":"additional","affiliation":[{"name":"Universite\u0301 Bourgogne Europe, CNRS, ICMUB UMR 6302 , 9 Avenue Alain Savary , ,","place":["Dijon, France, 21000"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,15]]},"reference":[{"issue":"5","key":"2026092911101187100_cit1","doi-asserted-by":"publisher","first-page":"2641","DOI":"10.1021\/cr900343z","article-title":"Fluorescence Lifetime Measurements and Biological Imaging","volume":"110","author":"Berezin","year":"2010","journal-title":"Chem. 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Opt."},{"issue":"22","key":"2026092911101187100_cit8","doi-asserted-by":"publisher","first-page":"6653","DOI":"10.1158\/1078-0432.CCR-19-1686","article-title":"Fluorescence\nLifetime-Based Tumor Contrast Enhancement Using an EGFR Antibody\u2013Labeled\nNear-Infrared Fluorophore","volume":"25","author":"Pal","year":"2019","journal-title":"Clin. Cancer Res."},{"key":"2026092911101187100_cit9","doi-asserted-by":"publisher","first-page":"1649","DOI":"10.1038\/s41551-023-01105-2","article-title":"Fluorescence Lifetime of Injected Indocyanine\nGreen as a Universal Marker of Solid Tumours in Patients","volume":"7","author":"Pal","year":"2023","journal-title":"Nat. Biomed. Eng."},{"issue":"3","key":"2026092911101187100_cit10","doi-asserted-by":"publisher","first-page":"1352","DOI":"10.1039\/D5CS00280J","article-title":"Chemical Fluorophores for Fluorescence\nLifetime Imaging","volume":"55","author":"Lim","year":"2026","journal-title":"Chem. Soc. 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Rev."},{"issue":"5","key":"2026092911101187100_cit13","doi-asserted-by":"publisher","first-page":"3679","DOI":"10.1021\/acs.jmedchem.3c02139","article-title":"Comparison\nof the In Vitro and In\nVivo Behavior of a Series of NIR-II-Emitting Aza-BODIPYs Containing\nDifferent Water-Solubilizing Groups and Their Trastuzumab Antibody\nConjugates","volume":"67","author":"Chazeau","year":"2024","journal-title":"J. Med. Chem."},{"issue":"1","key":"2026092911101187100_cit14","doi-asserted-by":"publisher","first-page":"669","DOI":"10.1021\/jo201413b","article-title":"Replacing\nPhenyl Ring with Thiophene: An Approach to\nLonger Wavelength Aza-Dipyrromethene Boron Difluoride (Aza-BODIPY)\nDyes","volume":"77","author":"Zhang","year":"2012","journal-title":"J. Org. Chem."},{"issue":"4","key":"2026092911101187100_cit15","doi-asserted-by":"publisher","first-page":"1061","DOI":"10.1021\/acs.bioconjchem.8b00795","article-title":"A Promising Family\nof Fluorescent Water-Soluble Aza-BODIPY Dyes for\nin Vivo Molecular Imaging","volume":"30","author":"Pliquett","year":"2019","journal-title":"Bioconjugate Chem."},{"issue":"9","key":"2026092911101187100_cit16","doi-asserted-by":"publisher","first-page":"796","DOI":"10.1021\/cbmi.3c00091","article-title":"Chemical Regulation\nof Fluorescence Lifetime","volume":"1","author":"Dai","year":"2023","journal-title":"Chem. Biomed. 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Med.\nChem."},{"issue":"3","key":"2026092911101187100_cit18","doi-asserted-by":"publisher","first-page":"569","DOI":"10.1021\/bc400574z","article-title":"Transglutaminase-Based Chemo-Enzymatic Conjugation\nApproach Yields Homogeneous Antibody\u2013Drug Conjugates","volume":"25","author":"Dennler","year":"2014","journal-title":"Bioconjugate Chem."},{"issue":"6","key":"2026092911101187100_cit19","doi-asserted-by":"publisher","first-page":"3073","DOI":"10.1021\/acs.molpharmaceut.3c00085","article-title":"RAFT Polymer\u2013Antibody\nConjugation: Squaramide\nEster Chemistry Leads to Conjugates with a Therapeutic Anti-EGFR Antibody\nwith Full Retention of Activity and Increased Tumor Uptake In Vivo","volume":"20","author":"Ardana","year":"2023","journal-title":"Mol. Pharmaceutics"},{"issue":"7","key":"2026092911101187100_cit20","doi-asserted-by":"publisher","first-page":"1223","DOI":"10.1021\/acs.bioconjchem.1c00305","article-title":"Squaric Acid-Based Radiopharmaceuticals for Tumor Imaging\nand Therapy","volume":"32","author":"Grus","year":"2021","journal-title":"Bioconjugate Chem."},{"issue":"9","key":"2026092911101187100_cit21","doi-asserted-by":"publisher","first-page":"3496","DOI":"10.1016\/j.ejmech.2009.01.019","article-title":"An in Vitro Characterization Study\nof New near Infrared Dyes for Molecular Imaging","volume":"44","author":"Pauli","year":"2009","journal-title":"Eur. J. Med. Chem."},{"issue":"8","key":"2026092911101187100_cit22","doi-asserted-by":"publisher","first-page":"2402","DOI":"10.1039\/c3cs60368g","article-title":"The Role of \u201cDisaggregation\u201d\nin Optical Probe Development","volume":"43","author":"Zhai","year":"2014","journal-title":"Chem. Soc. Rev."},{"issue":"34","key":"2026092911101187100_cit23","doi-asserted-by":"publisher","first-page":"10619","DOI":"10.1021\/ja047649e","article-title":"In Vitro\nDemonstration of the Heavy-Atom Effect for\nPhotodynamic Therapy","volume":"126","author":"Gorman","year":"2004","journal-title":"J. Am. Chem. Soc."},{"issue":"12","key":"2026092911101187100_cit24","doi-asserted-by":"publisher","first-page":"2213","DOI":"10.1351\/PAC-REP-10-09-31","article-title":"Standards\nfor photoluminescence quantum yield measurements in solution (IUPAC\nTechnical Report)","volume":"83","author":"Brouwer","year":"2011","journal-title":"Pure Appl. Chem."},{"issue":"4","key":"2026092911101187100_cit25","doi-asserted-by":"publisher","DOI":"10.1002\/smm2.1245","article-title":"NIR-II Cyanine@albumin Fluorophore for Deep Tissue\nImaging and Imaging-Guided Surgery","volume":"5","author":"Zhang","year":"2024","journal-title":"SmartMater"},{"issue":"5","key":"2026092911101187100_cit26","doi-asserted-by":"publisher","first-page":"416","DOI":"10.4161\/cbt.10.5.12610","article-title":"EGFR Expression Variance\nin Paired Colorectal Cancer Primary and Metastatic Tumors","volume":"10","author":"Yarom","year":"2010","journal-title":"Cancer Biol. Ther."},{"issue":"3","key":"2026092911101187100_cit27","doi-asserted-by":"publisher","first-page":"530","DOI":"10.1021\/acs.bioconjchem.2c00049","article-title":"Site-Specifi, Platform-Based Conjugation\nStrategy for the Synthesis of Dual-Labeled Immunoconjugates for Bimodal\nPET\/NIRF Imaging of HER2-Positive Tumors","volume":"33","author":"Adumeau","year":"2022","journal-title":"Bioconjugate\nChem."},{"issue":"4","key":"2026092911101187100_cit28","doi-asserted-by":"publisher","first-page":"621","DOI":"10.1021\/acs.bioconjchem.3c00080","article-title":"First Comparison Study\nof the In Vitro and In Vivo Properties of\na Randomly and Site-Specifically Conjugated SPECT\/NIRF Monomolecular\nMultimodal Imaging Probe (MOMIP) Based on an Aza-BODIPY Fluorophore","volume":"34","author":"Privat","year":"2023","journal-title":"Bioconjugate Chem."},{"issue":"3","key":"2026092911101187100_cit29","doi-asserted-by":"publisher","first-page":"2188","DOI":"10.1021\/acs.jmedchem.3c02120","article-title":"Development\nof an Immuno-SPECT\/Fluorescent\nBimodal Tracer Targeting Human or Murine PD-L1 on Preclinical Models","volume":"67","author":"Privat","year":"2024","journal-title":"J. Med. Chem."},{"key":"2026092911101187100_cit30","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1007\/978-1-4939-9546-2_8","article-title":"Site-Specific\nAntibody\u2013Drug Conjugation Using Microbial Transglutaminase","volume":"2012","author":"Dickgiesser","year":"2019","journal-title":"Methods Mol. Biol."},{"issue":"24","key":"2026092911101187100_cit31","doi-asserted-by":"publisher","first-page":"43900","DOI":"10.1109\/JSEN.2025.3626602","article-title":"128 \u00d7 128-Pixel Current-Assisted Photonic Sampler\nImage Sensor and Camera System for Macroscale Fluorescence Lifetime\nImaging With Multiexposure for Extended Dynamic Range","volume":"25","author":"Van den Dries","year":"2025","journal-title":"IEEE Sens. J."},{"issue":"4","key":"2026092911101187100_cit32","doi-asserted-by":"publisher","first-page":"046011","DOI":"10.1117\/1.3469797","article-title":"Lifetime-Based\nTomographic Multiplexing","volume":"15","author":"Raymond","year":"2010","journal-title":"J. Biomed. Opt."},{"issue":"2","key":"2026092911101187100_cit33","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1021\/ac60146a018","article-title":"Determination of\nWater by Titration Wth Coulometrically\nGenerated Karl Fischer Reagent","volume":"31","author":"Meyer","year":"1959","journal-title":"Anal. Chem."},{"issue":"4","key":"2026092911101187100_cit34","doi-asserted-by":"publisher","first-page":"1953","DOI":"10.1364\/BOE.587556","article-title":"Defining the optimal imaging time point for fluorescence-lifetime-based\ntumor identification using the non-targeting near-infrared dye indocyanine\ngreen and post-processed high-dynamic-range images","volume":"17","author":"Janssen","year":"2026","journal-title":"Biomed. Opt. Express"}],"container-title":["ACS Omega"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsomega.6c04430","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/acsodf\/article-pdf\/11\/38\/57061\/68525150\/acsomega.6c04430.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/acsodf\/article-pdf\/11\/38\/57061\/68525150\/acsomega.6c04430.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T16:10:10Z","timestamp":1790698210000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/acsodf\/article\/11\/38\/57061\/5430862\/Near-Infrared-Organic-Structures-with-Extended"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,15]]},"references-count":34,"journal-issue":{"issue":"38","published-online":{"date-parts":[[2026,9,15]]},"published-print":{"date-parts":[[2026,9,29]]}},"URL":"https:\/\/doi.org\/10.1021\/acsomega.6c04430","relation":{"has-preprint":[{"id-type":"doi","id":"10.26434\/chemrxiv-2025-hfw7h","asserted-by":"object"}]},"ISSN":["2470-1343"],"issn-type":[{"value":"2470-1343","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,15]]}}}