{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T16:25:20Z","timestamp":1786465520797,"version":"build-2736575974"},"reference-count":49,"publisher":"American Chemical Society (ACS)","issue":"31","license":[{"start":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T00:00:00Z","timestamp":1784937600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Tumor necrosis factor \u03b1 (TNF\u03b1) is a proinflammatory cytokine associated with autoimmune disorders such as Crohn\u2019s disease, rheumatoid arthritis, and psoriasis. Biotherapeutics represent a rapidly growing part of the treatments used to address autoimmune conditions. Therapeutic monoclonal antibodies (mAbs) are designed to neutralize TNF\u03b1 but exhibit various efficacies. These variations suggest subtle differences in mAbs binding or neutralization mechanisms. TNF\u03b1 can bind two receptors, TNFR1 and TNFR2. Therapeutic mAbs bind to TNF\u03b1 and inhibit signaling through both receptors. X-ray crystallography suggests that when mAbs bind to TNF\u03b1, neutralization occurs through steric hindrance. In this report, we used hydrogen\/deuterium exchange mass spectrometry (HDX-MS) to analyze TNF\u03b1 bound to three mAbs. Our results revealed a significant allosteric reduction in TNF\u03b1 dynamic motion after binding mAbs and receptors. These findings provide an improved model for therapeutic mAbs binding and neutralization of TNF\u03b1 that incorporates both steric and allosteric mechanisms. Using dynamic structural biology techniques such as HDX-MS to identify new details about neutralizing mAbs, TNFR1, and TNFR2 binding to TNF\u03b1 is an important advance for biotherapeutic development. Understanding and improving signal neutralization mechanisms may ultimately help minimize symptoms in individuals with autoimmune disorders.<\/jats:p>","DOI":"10.1021\/acsomega.6c04448","type":"journal-article","created":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T13:05:17Z","timestamp":1785848717000},"page":"46804-46819","source":"Crossref","is-referenced-by-count":0,"title":["HDX-MS Detects\nSteric Protection and Trimeric Compaction\nof TNF\u03b1 after It Binds Receptors or Antibodies"],"prefix":"10.1021","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5590-1784","authenticated-orcid":true,"given":"Christopher A.","family":"Haynes","sequence":"first","affiliation":[{"name":"Protein Structure Laboratory, Clinical Chemistry Branch, Division ofLaboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention , 4770 Buford Hwy , , ,","place":["Atlanta, Georgia, United States, 30341"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0779-315X","authenticated-orcid":true,"given":"Theodore R.","family":"Keppel","sequence":"additional","affiliation":[{"name":"Protein Structure Laboratory, Clinical Chemistry Branch, Division ofLaboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention , 4770 Buford Hwy , , ,","place":["Atlanta, Georgia, United States, 30341"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5311-1631","authenticated-orcid":true,"given":"Sarah H.","family":"Osman","sequence":"additional","affiliation":[{"name":"Protein Structure Laboratory, Clinical Chemistry Branch, Division ofLaboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention , 4770 Buford Hwy , , ,","place":["Atlanta, Georgia, United States, 30341"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Betlehem","family":"Mekonnen","sequence":"additional","affiliation":[{"name":"Protein Structure Laboratory, Clinical Chemistry Branch, Division ofLaboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention , 4770 Buford Hwy , , ,","place":["Atlanta, Georgia, United States, 30341"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jakub","family":"Baudys","sequence":"additional","affiliation":[{"name":"Protein Structure Laboratory, Clinical Chemistry Branch, Division ofLaboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention , 4770 Buford Hwy , , ,","place":["Atlanta, Georgia, United States, 30341"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian R.","family":"Woolfitt","sequence":"additional","affiliation":[{"name":"Protein Structure Laboratory, Clinical Chemistry Branch, Division ofLaboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention , 4770 Buford Hwy , , ,","place":["Atlanta, Georgia, United States, 30341"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John R.","family":"Barr","sequence":"additional","affiliation":[{"name":"Protein Structure Laboratory, Clinical Chemistry Branch, Division ofLaboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention , 4770 Buford Hwy , , ,","place":["Atlanta, Georgia, United States, 30341"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0993-1018","authenticated-orcid":true,"given":"Dongxia","family":"Wang","sequence":"additional","affiliation":[{"name":"Protein Structure Laboratory, Clinical Chemistry Branch, Division ofLaboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention , 4770 Buford Hwy , , ,","place":["Atlanta, Georgia, United States, 30341"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,7,25]]},"reference":[{"issue":"2","key":"2026081110194928800_cit1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2025.104299","article-title":"Death ligand receptor\n(DLR) signaling: Its non-apoptotic functions in cancer and the consequences\nof DLR-directed therapies","volume":"30","author":"Rashid","year":"2025","journal-title":"Drug Discovery Today."},{"issue":"11","key":"2026081110194928800_cit2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20240806","article-title":"Targeting TNF\/TNFR\nsuperfamilies in immune-mediated inflammatory diseases","volume":"221","author":"Veerasubramanian","year":"2024","journal-title":"J. Exp. Med."},{"key":"2026081110194928800_cit3","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2024.155665","article-title":"TNF-alpha,\nand TNFRs in gastrointestinal cancers","volume":"263","author":"Mozooni","year":"2024","journal-title":"Pathol.,\nRes. Pract."},{"key":"2026081110194928800_cit4","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2024.1430187","article-title":"TGF-beta and TNF-alpha interaction promotes the expression\nof MMP-9 through H3K36 dimethylation: implications in breast cancer\nmetastasis","volume":"15","author":"Kochumon","year":"2024","journal-title":"Front. Immunol."},{"key":"2026081110194928800_cit5","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.17023.1","article-title":"Tumour necrosis\nfactor signalling in health and disease","volume":"8","author":"Holbrook","year":"2019","journal-title":"F1000Research"},{"issue":"1","key":"2026081110194928800_cit6","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1080\/1744666X.2024.2400300","article-title":"Translational characterization\nof immune pathways in inflammatory bowel disease: insights for targeted\ntreatments","volume":"21","author":"Nicolo\u0300","year":"2025","journal-title":"Expert Rev. Clin. Immunol."},{"issue":"8","key":"2026081110194928800_cit7","doi-asserted-by":"publisher","first-page":"609","DOI":"10.1038\/s41435-019-0057-0","article-title":"The many faces of tumor necrosis factor signaling in\nthe intestinal epithelium","volume":"20","author":"Delgado","year":"2019","journal-title":"Genes Immun."},{"issue":"4","key":"2026081110194928800_cit8","doi-asserted-by":"publisher","first-page":"622","DOI":"10.1111\/imm.13855","article-title":"Towards understanding the role of nanomedicine in targeting\nTNFR2 in rheumatoid arthritis","volume":"173","author":"Lambuk","year":"2024","journal-title":"Immunology"},{"key":"2026081110194928800_cit9","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.cytogfr.2024.06.001","article-title":"Targeting cytokines\nin psoriatic arthritis","volume":"78","author":"Neurath","year":"2024","journal-title":"Cytokine Growth Factor\nRev."},{"issue":"38","key":"2026081110194928800_cit10","doi-asserted-by":"publisher","first-page":"27059","DOI":"10.1074\/jbc.M113.491530","article-title":"Comparison\nof the inhibition mechanisms of adalimumab\nand infliximab in treating tumor necrosis factor alpha-associated\ndiseases from a molecular view","volume":"288","author":"Hu","year":"2013","journal-title":"J. Biol. Chem."},{"issue":"19","key":"2026081110194928800_cit11","doi-asserted-by":"publisher","first-page":"13799","DOI":"10.1074\/jbc.M112.433961","article-title":"Structural basis for treating tumor necrosis factor alpha (TNFalpha)-associated\ndiseases with the therapeutic antibody infliximab","volume":"288","author":"Liang","year":"2013","journal-title":"J. Biol. 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Pharm."},{"issue":"9","key":"2026081110194928800_cit15","doi-asserted-by":"publisher","DOI":"10.3390\/medicina60091409","article-title":"Neurological\nSide Effects of TNF-alpha Inhibitors Revisited:\nA Review of Case Reports","volume":"60","author":"Gogulescu","year":"2024","journal-title":"Medicina"},{"issue":"5","key":"2026081110194928800_cit16","doi-asserted-by":"publisher","first-page":"793","DOI":"10.1016\/0092-8674(95)90192-2","article-title":"The transmembrane form of tumor necrosis factor\nis the prime activating ligand of the 80 kDa tumor necrosis factor\nreceptor","volume":"83","author":"Grell","year":"1995","journal-title":"Cell"},{"issue":"11","key":"2026081110194928800_cit17","doi-asserted-by":"publisher","first-page":"4184","DOI":"10.1182\/blood.V86.11.4184.bloodjournal86114184","article-title":"Critical involvement of transmembrane tumor necrosis\nfactor-alpha in endothelial programmed cell death mediated by ionizing\nradiation and bacterial endotoxin","volume":"86","author":"Eissner","year":"1995","journal-title":"Blood"},{"key":"2026081110194928800_cit18","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.1042622","article-title":"The dichotomous outcomes of TNFalpha\nsignaling in CD4(+) T cells","volume":"13","author":"Skartsis","year":"2022","journal-title":"Front. Immunol."},{"issue":"1","key":"2026081110194928800_cit19","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-025-06122-0","article-title":"Exploring TNFR1: from\ndiscovery to targeted therapy development","volume":"23","author":"Li","year":"2025","journal-title":"J.\nTransl. Med."},{"issue":"1","key":"2026081110194928800_cit20","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1002\/ijc.35130","article-title":"The implications\nof the TNF\u03b1-TNFR2 immune checkpoint signaling pathway in cancer\ntreatment: From immunoregulation to angiogenesis","volume":"156","author":"Emamalipour","year":"2025","journal-title":"Int. J. Cancer"},{"issue":"24","key":"2026081110194928800_cit21","doi-asserted-by":"publisher","DOI":"10.3390\/cells13242092","article-title":"Updates on Inflammatory Molecular Pathways Mediated by ADAM17 in\nAutoimmunity","volume":"13","author":"Sisto","year":"2024","journal-title":"Cells"},{"issue":"4","key":"2026081110194928800_cit22","doi-asserted-by":"publisher","first-page":"1537","DOI":"10.1172\/JCI45005","article-title":"Soluble TNFRp75 regulates host protective immunity\nagainst Mycobacterium tuberculosis","volume":"124","author":"Keeton","year":"2014","journal-title":"J. Clin.\nInvest."},{"issue":"7","key":"2026081110194928800_cit23","doi-asserted-by":"publisher","first-page":"1303","DOI":"10.1021\/jasms.2c00108","article-title":"Complementary Structural Information\nfor Antibody-Antigen Complexes from Hydrogen-Deuterium Exchange and\nCovalent Labeling Mass Spectrometry","volume":"33","author":"Tremblay","year":"2022","journal-title":"J. Am.\nSoc. Mass Spectrom."},{"issue":"4","key":"2026081110194928800_cit24","doi-asserted-by":"publisher","first-page":"1221","DOI":"10.1016\/j.jmb.2008.11.053","article-title":"Structure-function\nrelationship of tumor necrosis factor\n(TNF) and its receptor interaction based on 3D structural analysis\nof a fully active TNFR1-selective TNF mutant","volume":"385","author":"Mukai","year":"2009","journal-title":"J. Mol. Biol."},{"issue":"1","key":"2026081110194928800_cit25","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-20828-3","article-title":"Structural insights\ninto the disruption of TNF-TNFR1 signalling by small molecules stabilising\na distorted TNF","volume":"12","author":"McMillan","year":"2021","journal-title":"Nat. Commun."},{"issue":"148","key":"2026081110194928800_cit26","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.2000954","article-title":"Solution\nof the structure of the TNF-TNFR2\ncomplex","volume":"3","author":"Mukai","year":"2010","journal-title":"Sci Signal."},{"issue":"10","key":"2026081110194928800_cit27","doi-asserted-by":"publisher","first-page":"1101","DOI":"10.1093\/protein\/10.10.1101","article-title":"Crystal\nstructure of TNF-alpha mutant\nR31D with greater affinity for receptor R1 compared with R2","volume":"10","author":"Reed","year":"1997","journal-title":"Protein Eng."},{"issue":"4","key":"2026081110194928800_cit28","doi-asserted-by":"publisher","first-page":"827","DOI":"10.1002\/j.1460-2075.1991.tb08015.x","article-title":"Localization\nof the active site of human tumour necrosis factor (hTNF) by mutational\nanalysis","volume":"10","author":"Van Ostade","year":"1991","journal-title":"EMBO J."},{"issue":"3","key":"2026081110194928800_cit29","doi-asserted-by":"publisher","first-page":"431","DOI":"10.1016\/0092-8674(93)90132-A","article-title":"Crystal structure of the soluble human 55 kd TNF receptor-human\nTNF beta complex: implications for TNF receptor activation","volume":"73","author":"Banner","year":"1993","journal-title":"Cell."},{"key":"2026081110194928800_cit30","first-page":"274","article-title":"Allostery","author":"Walker","year":"2021","edition":"1"},{"key":"2026081110194928800_cit31","doi-asserted-by":"publisher","first-page":"1335","DOI":"10.1016\/j.csbj.2025.03.046","article-title":"Targeting\nthe inter-monomeric space of TNFR1 pre-ligand dimers: A novel binding\npocket for allosteric modulators","volume":"27","author":"Lo","year":"2025","journal-title":"Comput. Struct.\nBiotechnol. J."},{"issue":"8","key":"2026081110194928800_cit32","doi-asserted-by":"publisher","first-page":"1327","DOI":"10.1080\/19420862.2017.1379641","article-title":"Suppressing allostery\nin epitope mapping experiments\nusing millisecond hydrogen\/deuterium exchange mass spectrometry","volume":"9","author":"Deng","year":"2017","journal-title":"MAbs"},{"issue":"8","key":"2026081110194928800_cit33","doi-asserted-by":"publisher","first-page":"1514","DOI":"10.1016\/j.jasms.2009.04.006","article-title":"Protein\nconformations can be probed in top-down HDX MS experiments utilizing\nelectron transfer dissociation of protein ions without hydrogen scrambling","volume":"20","author":"Abzalimov","year":"2009","journal-title":"J. Am. Soc. Mass Spectrom."},{"issue":"5","key":"2026081110194928800_cit34","doi-asserted-by":"publisher","first-page":"3079","DOI":"10.1021\/acs.analchem.7b03655","article-title":"Top-Down\nHydrogen-Deuterium Exchange Analysis of Protein\nStructures Using Ultraviolet Photodissociation","volume":"90","author":"Brodie","year":"2018","journal-title":"Anal. Chem."},{"issue":"11","key":"2026081110194928800_cit35","doi-asserted-by":"publisher","first-page":"6409","DOI":"10.1021\/acs.analchem.8b00429","article-title":"Automated Removal\nof Phospholipids\nfrom Membrane Proteins for H\/D Exchange Mass Spectrometry Workflows","volume":"90","author":"Anderson","year":"2018","journal-title":"Anal. Chem."},{"issue":"3","key":"2026081110194928800_cit36","doi-asserted-by":"publisher","first-page":"623","DOI":"10.1007\/s13361-017-1860-3","article-title":"Optimization of\nFeasibility Stage for Hydrogen\/Deuterium\nExchange Mass Spectrometry","volume":"29","author":"Hamuro","year":"2018","journal-title":"J. Am. Soc. Mass\nSpectrom."},{"issue":"11","key":"2026081110194928800_cit37","doi-asserted-by":"publisher","first-page":"5000","DOI":"10.1021\/acs.analchem.2c05310","article-title":"Online Fully Automated\nSystem for Hydrogen\/Deuterium-Exchange\nMass Spectrometry with Millisecond Time Resolution","volume":"95","author":"Kish","year":"2023","journal-title":"Anal. Chem."},{"issue":"11","key":"2026081110194928800_cit38","doi-asserted-by":"publisher","first-page":"2305","DOI":"10.1021\/jasms.0c00258","article-title":"Deglycosylation by the Acidic Glycosidase PNGase\nH(+) Enables Analysis of N-Linked Glycoproteins by Hydrogen\/Deuterium\nExchange Mass Spectrometry","volume":"31","author":"Comamala","year":"2020","journal-title":"J. Am. Soc. Mass\nSpectrom."},{"issue":"13","key":"2026081110194928800_cit39","doi-asserted-by":"publisher","first-page":"6681","DOI":"10.1021\/acs.analchem.5b00831","article-title":"Recombinant Nepenthesin II for Hydrogen\/Deuterium Exchange\nMass Spectrometry","volume":"87","author":"Yang","year":"2015","journal-title":"Anal. Chem."},{"issue":"9","key":"2026081110194928800_cit40","doi-asserted-by":"publisher","first-page":"2386","DOI":"10.1021\/jasms.1c00080","article-title":"Rapid Assessment of Pepsin Column\nActivity for Reliable HDX-MS Studies","volume":"32","author":"Vorauer","year":"2021","journal-title":"J. Am.\nSoc. Mass Spectrom."},{"issue":"7","key":"2026081110194928800_cit41","doi-asserted-by":"publisher","first-page":"1461","DOI":"10.1021\/jasms.4c00056","article-title":"Evaluation of Proteolytic Digestion\nEfficiency in Hydrogen Exchange-Mass Spectrometry Experiments Using\nthe Digestible Peptide Score","volume":"35","author":"Wrigley","year":"2024","journal-title":"J. Am. Soc. Mass\nSpectrom."},{"issue":"2","key":"2026081110194928800_cit42","doi-asserted-by":"publisher","first-page":"270","DOI":"10.1093\/bioinformatics\/btaa677","article-title":"Deuteros 2.0: peptide-level\nsignificance testing of data from hydrogen deuterium exchange mass\nspectrometry","volume":"37","author":"Lau","year":"2021","journal-title":"Bioinformatics."},{"issue":"1","key":"2026081110194928800_cit43","doi-asserted-by":"publisher","DOI":"10.1186\/s12859-014-0403-1","article-title":"QUDeX-MS:\nhydrogen\/deuterium exchange calculation for\nmass spectra with resolved isotopic fine structure","volume":"15","author":"Salisbury","year":"2014","journal-title":"BMC Bioinf."},{"issue":"15","key":"2026081110194928800_cit44","doi-asserted-by":"publisher","first-page":"3493","DOI":"10.1021\/acs.jpcb.6b09358","article-title":"A Residue-Resolved Bayesian Approach to Quantitative\nInterpretation of Hydrogen-Deuterium Exchange from Mass Spectrometry:\nApplication to Characterizing Protein-Ligand Interactions","volume":"121","author":"Saltzberg","year":"2017","journal-title":"J Phys Chem B."},{"issue":"21","key":"2026081110194928800_cit45","doi-asserted-by":"publisher","first-page":"12242","DOI":"10.1021\/acs.chemrev.4c00438","article-title":"Computational Tools\nfor Hydrogen-Deuterium Exchange\nMass Spectrometry Data Analysis","volume":"124","author":"Stofella","year":"2024","journal-title":"Chem. Rev."},{"issue":"1","key":"2026081110194928800_cit46","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2025.108166","article-title":"RosettaHDX: Predicting antibody-antigen interaction\nfrom hydrogen-deuterium exchange mass spectrometry data","volume":"217","author":"Tran","year":"2025","journal-title":"J Struct. Biol."},{"issue":"2","key":"2026081110194928800_cit47","doi-asserted-by":"publisher","first-page":"416","DOI":"10.1021\/jasms.4c00471","article-title":"Rigorous\nAnalysis of Multimodal HDX-MS Spectra","volume":"36","author":"Tuttle","year":"2025","journal-title":"J. Am.\nSoc. Mass Spectrom."},{"issue":"7","key":"2026081110194928800_cit48","doi-asserted-by":"publisher","first-page":"595","DOI":"10.1038\/s41592-019-0459-y","article-title":"Recommendations for performing, interpreting and reporting\nhydrogen deuterium exchange mass spectrometry (HDX-MS) experiments","volume":"16","author":"Masson","year":"2019","journal-title":"Nat. Methods"},{"issue":"8","key":"2026081110194928800_cit49","doi-asserted-by":"publisher","first-page":"1627","DOI":"10.1021\/ac60214a047","article-title":"Smoothing and Differentiation of\nData by Simplified\nLeast Squares Procedures","volume":"36","author":"Savitzky","year":"1964","journal-title":"Anal. Chem."}],"container-title":["ACS Omega"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsomega.6c04448","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/acsodf\/article-pdf\/11\/31\/46804\/66253276\/acsomega.6c04448.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/acsodf\/article-pdf\/11\/31\/46804\/66253276\/acsomega.6c04448.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T15:01:50Z","timestamp":1786460510000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/acsodf\/article\/11\/31\/46804\/5232221\/HDX-MS-Detects-Steric-Protection-and-Trimeric"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,25]]},"references-count":49,"journal-issue":{"issue":"31","published-online":{"date-parts":[[2026,7,25]]},"published-print":{"date-parts":[[2026,8,11]]}},"URL":"https:\/\/doi.org\/10.1021\/acsomega.6c04448","relation":{},"ISSN":["2470-1343"],"issn-type":[{"value":"2470-1343","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,25]]}}}