{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:13:07Z","timestamp":1788221587284,"version":"build-2803163510"},"reference-count":50,"publisher":"American Chemical Society (ACS)","issue":"33","license":[{"start":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T00:00:00Z","timestamp":1786320000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100031478","name":"NextGenerationEU","doi-asserted-by":"publisher","award":["2022 PNRR P2022973R3"],"award-info":[{"award-number":["2022 PNRR P2022973R3"]}],"id":[{"id":"10.13039\/100031478","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The biological properties of microbial melanins, whose structures resemble those of animal-derived eumelanin pigments, have been poorly investigated in mammalian cell systems, as well as the role of amyloid fibrils in modulating melanin activities. Indeed, although amyloid fibrils are known to be crucial in the melanogenesis process, their functional role is not fully understood. In this study, we have investigated, for the first time, the ability of a microbial eumelanin, biotechnologically produced and purified by Streptomyces nashvillensis DSM 40314, to act as a protective agent against oxidative stress, UV radiation damage, and cytotoxicity associated with advanced glycation end products (AGEs) in a human keratinocyte cell model. Also, the effect of amyloid fibrils on the structural and biological properties of microbial melanin has been investigated for the first time, and the results indicated that the addition of an amyloid scaffold promotes an increase of melanin antioxidant activity and provides greater cellular protection against UV irradiation and AGE-induced cytotoxicity, key events associated with skin damage and aging. This study provides new insights into the functional role of amyloid in enhancing the protective properties of biotechnologically produced microbial melanin, highlighting its potential as a substitute for animal-derived eumelanin.<\/jats:p>","DOI":"10.1021\/acsomega.6c05125","type":"journal-article","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T17:02:12Z","timestamp":1786381332000},"page":"50028-50039","source":"Crossref","is-referenced-by-count":0,"title":["Role of Amyloid\nFibrils in Enhancing the Protective\nProperties of Microbial Melanin against Oxidative Stress, UV Damage,\nand AGE-Related Cytotoxicity in Human Keratinocytes"],"prefix":"10.1021","volume":"11","author":[{"given":"Shana","family":"Perrella","sequence":"first","affiliation":[{"name":"University of Campania \u201cLuigi Vanvitelli\u201d , , Via L. De Crecchio 7 , ,","place":["Naples, Italy, 80138"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Talayeh","family":"Kordjazi","sequence":"additional","affiliation":[{"name":"University of Naples Federico II , , Monte Sant\u2019Angelo Campus, Via Cintia 4 , ,","place":["Naples, Italy, 80126"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Liccardo","sequence":"additional","affiliation":[{"name":"University of Campania \u201cLuigi Vanvitelli\u201d , , Via L. De Crecchio 7 , ,","place":["Naples, Italy, 80138"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6800-5890","authenticated-orcid":true,"given":"Loredana","family":"Mariniello","sequence":"additional","affiliation":[{"name":"University of Naples Federico II , , Monte Sant\u2019Angelo Campus, Via Cintia 4 , ,","place":["Naples, Italy, 80126"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivana","family":"Sirangelo","sequence":"additional","affiliation":[{"name":"University of Campania \u201cLuigi Vanvitelli\u201d , , Via L. De Crecchio 7 , ,","place":["Naples, Italy, 80138"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-9617-9166","authenticated-orcid":true,"given":"Odile Francesca","family":"Restaino","sequence":"additional","affiliation":[{"name":"University of Naples Federico II , , Monte Sant\u2019Angelo Campus, Via Cintia 4 , ,","place":["Naples, Italy, 80126"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara","family":"Iannuzzi","sequence":"additional","affiliation":[{"name":"University of Campania \u201cLuigi Vanvitelli\u201d , , Via L. 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Biotechnol."},{"key":"2026083119152291200_cit6","doi-asserted-by":"publisher","first-page":"7265","DOI":"10.1007\/s00253-022-12191-8","article-title":"Enhanced Streptomyces roseochromogenes melanin production by using the marine renewable source Posidonia oceanica egagropili","volume":"106","author":"Restaino","year":"2022","journal-title":"Appl.\nMicrobiol. Biotechnol."},{"key":"2026083119152291200_cit7","doi-asserted-by":"publisher","first-page":"297","DOI":"10.3390\/microorganisms12020297","article-title":"Biotechnological production\nand characterization\nof extracellular melanin by Streptomyces nashvillensis","volume":"12","author":"Restaino","year":"2024","journal-title":"Microorganisms"},{"key":"2026083119152291200_cit8","doi-asserted-by":"publisher","first-page":"416","DOI":"10.3390\/ijms26010416","article-title":"Metal ion\nsupplementation to boost melanin production by Streptomyces nashvillensis","volume":"26","author":"Restaino","year":"2025","journal-title":"Int. J. Mol. 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