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Its clinical utility is limited because of its poor solubility and bioavailability. Since oxidative stress is a cause of cataract formation, this study thus developed quercetin-loaded nanoemulsion (QNE) eye drops and further improved their efficacy by preparing in a form of chitosan-coated QNE (CS-QNE). It was found that CS enlarged the droplet size of QNE to 184.33 \u00b1 0.71 nm and shifted the \u03b6-potential from negative to positive values. CS-QNE had entrapment efficiency of 99.99% with a pH of 6.0 \u00b1 0.0, and shear-thinning behavior. Drug release study demonstrated that the release of quercetin from CS-QNE complied with a zero-order release kinetic. Furthermore, CS-QNE could effectively protect the HLE and HCE-T cells from H2O2-induced oxidative stress. It exhibited strong antioxidant activity with EC50 of 3.15 \u00b1 0.52 \u03bcg\/mL and 19.48 \u00b1 2.36 \u03bcg\/mL from the DPPH and ABTS assays, respectively. Cellular uptake studies revealed efficient internalization of CS-QNE in the HLE and HCE-T cells. CS-QNE also showed a trend toward increased Bcl-2 expression, which may contribute to reduced oxidative stress.. The findings suggest that CS-QNE has the ability to protect the HLE and HCE-T cells from oxidative stress that relates to some ocular diseases, including cataract.<\/jats:p>","DOI":"10.1021\/acsomega.6c05355","type":"journal-article","created":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T18:01:55Z","timestamp":1790272915000},"page":"59339-59350","source":"Crossref","is-referenced-by-count":0,"title":["Chitosan-Coated\nQuercetin Nanoemulsion Eye Drops for\nSustained Ocular Delivery and Protection of Lens Degeneration from\nOxidative Stress"],"prefix":"10.1021","volume":"11","author":[{"given":"Papawee","family":"Sookdee","sequence":"first","affiliation":[{"name":"Thammasat University , , ,","place":["Pathum Thani, Thailand, 12120"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3453-5022","authenticated-orcid":true,"given":"Sewan","family":"Theeramunkong","sequence":"additional","affiliation":[{"name":"Thammasat University , , ,","place":["Pathum Thani, Thailand, 12120"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asira","family":"Fuongfuchat","sequence":"additional","affiliation":[{"name":"National Science and Technology Development Agency (NSTDA) , , Thailand Science Park , ,","place":["Pathum Thani, Thailand, 12120"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Onmanee","family":"Prajuabjinda","sequence":"additional","affiliation":[{"name":"Thammasat University , , ,","place":["Pathum Thani, Thailand, 12120"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4580-9829","authenticated-orcid":true,"given":"Rathapon","family":"Asasutjarit","sequence":"additional","affiliation":[{"name":"Thammasat University , , ,","place":["Pathum Thani, Thailand, 12120"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,24]]},"reference":[{"key":"2026100610492584000_cit1","doi-asserted-by":"publisher","DOI":"10.3390\/biom15010151","article-title":"The Role of Quercetin,\na Flavonoid in the Management of Pathogenesis Through Regulation of\nOxidative Stress, Inflammation, and Biological Activities","volume":"15","author":"Alharbi","year":"2025","journal-title":"Biomolecules"},{"key":"2026100610492584000_cit2","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/6933812","article-title":"Protection of Human Lens Epithelial\nCells from Oxidative Stress Damage and Cell Apoptosis by KGF-2 through\nthe Akt\/Nrf2\/HO-1 Pathway","volume":"2022","author":"Liu","year":"2022","journal-title":"Oxid. 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Vis. Sci."},{"key":"2026100610492584000_cit41","doi-asserted-by":"publisher","DOI":"10.1007\/s10792-025-03782-1","article-title":"The Protective Role\nof Quercetin in Cataract Lenses and Its Mechanisms\nRelated to the Hippo Signaling Pathway","volume":"45","author":"Miao","year":"2025","journal-title":"Int.\nOphthalmol."},{"key":"2026100610492584000_cit42","doi-asserted-by":"publisher","DOI":"10.1016\/j.tice.2024.102465","article-title":"Quercetin Inhibits Hypertonicity-Induced\nInflammatory Injury in Human Corneal Epithelial Cells via the PTEN\/PI3K\/AKT\nPathway","volume":"89","author":"Huang","year":"2024","journal-title":"Tissue Cell"},{"issue":"8","key":"2026100610492584000_cit43","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22084056","article-title":"Protective Effect of\nQuercetin on Sodium Iodate-Induced Retinal Apoptosis\nthrough the Reactive Oxygen Species-Mediated Mitochondrion-Dependent\nPathway","volume":"22","author":"Chang","year":"2021","journal-title":"Int. J. 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