{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T16:10:19Z","timestamp":1790698219598,"version":"4.1.0"},"reference-count":47,"publisher":"American Chemical Society (ACS)","issue":"38","license":[{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003359","name":"Generalitat Valenciana","doi-asserted-by":"publisher","award":["AGROALNEXT_2022_060"],"award-info":[{"award-number":["AGROALNEXT_2022_060"]}],"id":[{"id":"10.13039\/501100003359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003359","name":"Generalitat Valenciana","doi-asserted-by":"publisher","award":["CIAPOS\/2023\/236"],"award-info":[{"award-number":["CIAPOS\/2023\/236"]}],"id":[{"id":"10.13039\/501100003359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003359","name":"Generalitat Valenciana","doi-asserted-by":"publisher","award":["IDIFEDER\/2018\/046"],"award-info":[{"award-number":["IDIFEDER\/2018\/046"]}],"id":[{"id":"10.13039\/501100003359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci??n y Universidades","doi-asserted-by":"publisher","award":["CEX2021-001189-S"],"award-info":[{"award-number":["CEX2021-001189-S"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci??n y Universidades","doi-asserted-by":"publisher","award":["CIDEXG\/2023\/20"],"award-info":[{"award-number":["CIDEXG\/2023\/20"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci??n y Universidades","doi-asserted-by":"publisher","award":["CNS2024-154560"],"award-info":[{"award-number":["CNS2024-154560"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci??n y Universidades","doi-asserted-by":"publisher","award":["PRTR-C17.I1"],"award-info":[{"award-number":["PRTR-C17.I1"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>This study addresses the sustainable valorization of persimmon (Diospyros kaki L.) side streams via process intensification through supercritical CO2 technology (Sc-CO2) (35 MPa, 50 \u00b0C, 120 min). Namely, the ethanol proportion in the supercritical stream and different levels of operation (LoOP) were tested. In particular, the process at a pilot LoOP (2%-ethanol-doped, 2 kg of sample) enabled the production of a lipophilic extract with enhanced nutritional and biological activity, making it a promising candidate for further research in nutraceutical formulations. More deeply, the extract demonstrated a noted antimicrobial activity against bacteria (Escherichia coli, Bacillus subtilis) and filamentous fungi (Penicillium expansum), with minimum inhibitory concentrations (MIC) ranging from 0.8-7-8 \u03bcL\/mL that were similar as the benchmark (oregano extract). In addition, the crude displayed a noted Fe content (85.8 mg\/100 g extract), with a relevant presence of Mg as well (up to 52% RDI in 100 g extract). The environmental sustainability of the process (pilot level, 2%-ethanol-doped) was assessed through green chemistry metrics. Namely, the protocol displayed 45.9 score in the eco-scale, and a ten-fold reduction for PMI or CEF compared to bench scale experiments (2%-ethanol). Overall, this work highlights the efficiency of an advanced methodology to produce a bioactive extract from persimmon culls that represents a promising candidate for the development of nutraceuticals.<\/jats:p>","DOI":"10.1021\/acsomega.6c05435","type":"journal-article","created":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T17:02:00Z","timestamp":1789578120000},"page":"57269-57280","source":"Crossref","is-referenced-by-count":0,"title":["Ethanol-Modified,\nSupercritical CO2 Extraction\nof Persimmon (\n                    <i>Diospyros kaki<\/i>\n                    L.) Side Streams for\nInnovative Bioactive and Antimicrobial Formulations: A Pilot Level\nProof-of-Concept and Green Chemistry Evaluation"],"prefix":"10.1021","volume":"11","author":[{"given":"Albert","family":"Sebastia\u0300","sequence":"first","affiliation":[{"name":"Universitat de Vale\u0300ncia , , Avenida Vicent Andre\u0301s Estelle\u0301s, 22 , , ,","place":["Burjassot, Valencia, Spain, 46100"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5775-6974","authenticated-orcid":true,"given":"Manuel","family":"Salgado-Ramos","sequence":"additional","affiliation":[{"name":"Universitat de Vale\u0300ncia , , Avenida Vicent Andre\u0301s Estelle\u0301s, 22 , , ,","place":["Burjassot, Valencia, Spain, 46100"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8018-3959","authenticated-orcid":true,"given":"Noelia","family":"Pallare\u0301s","sequence":"additional","affiliation":[{"name":"Universitat de Vale\u0300ncia , , Avenida Vicent Andre\u0301s Estelle\u0301s, 22 , , ,","place":["Burjassot, Valencia, Spain, 46100"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samantha","family":"Lo\u0301pez-Contreras","sequence":"additional","affiliation":[{"name":"Instituto de Agroqui\u0301mica y Tecnologi\u0301a de Alimentos (IATA), Consejo Superior de Investigaciones Cienti\u0301ficas (CSIC) , , , ,","place":["Paterna, Valencia, Spain, 46980"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Garrigues","sequence":"additional","affiliation":[{"name":"Instituto de Agroqui\u0301mica y Tecnologi\u0301a de Alimentos (IATA), Consejo Superior de Investigaciones Cienti\u0301ficas (CSIC) , , , ,","place":["Paterna, Valencia, Spain, 46980"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan A.","family":"Tamayo-Ramos","sequence":"additional","affiliation":[{"name":"Instituto de Agroqui\u0301mica y Tecnologi\u0301a de Alimentos (IATA), Consejo Superior de Investigaciones Cienti\u0301ficas (CSIC) , , , ,","place":["Paterna, Valencia, Spain, 46980"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raquel","family":"Lucas-Gonza\u0301lez","sequence":"additional","affiliation":[{"name":"Miguel Herna\u0301ndez University (CIAGRO-UMH) , , Carretera Beniel Km 3.2 , , ,","place":["Orihuela, Alicante, Spain, 03312"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4771-8437","authenticated-orcid":true,"given":"Juana","family":"Ferna\u0301ndez-Lo\u0301pez","sequence":"additional","affiliation":[{"name":"Miguel Herna\u0301ndez University (CIAGRO-UMH) , , Carretera Beniel Km 3.2 , , ,","place":["Orihuela, Alicante, Spain, 03312"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5630-3989","authenticated-orcid":true,"given":"Francisco J.","family":"Barba","sequence":"additional","affiliation":[{"name":"Universitat de Vale\u0300ncia , , Avenida Vicent Andre\u0301s Estelle\u0301s, 22 , , ,","place":["Burjassot, Valencia, Spain, 46100"]},{"name":"Instituto de Salud Carlos III , , C\/Monforte de Lemos 3\u20135. Pabello\u0301n 11. Planta 0 , ,","place":["Madrid, Spain, 28029"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,16]]},"reference":[{"issue":"1","key":"2026092911091974700_cit1","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1007\/s11130-016-0592-z","article-title":"Evaluation of particle size influence\non proximate composition, physicochemical, techno-functional and physio-functional\nproperties of flours obtained from persimmon (Diospyros kaki Trumb.) coproducts","volume":"72","author":"Lucas-Gonza\u0301lez","year":"2017","journal-title":"Plant Foods Hum.\nNutr."},{"key":"2026092911091974700_cit2","doi-asserted-by":"publisher","first-page":"504","DOI":"10.1002\/jsfa.8487","article-title":"Effect of\nparticle size on phytochemical composition\nand antioxidant properties of two persimmon flours from Diospyros kaki Thunb. Vars. \u2018Rojo Brillante\u2019 and \u2018Triumph\u2019\nco-products","volume":"98","author":"Lucas-Gonza\u0301lez","year":"2018","journal-title":"J. Sci. Food Agric."},{"issue":"5","key":"2026092911091974700_cit3","doi-asserted-by":"publisher","first-page":"1397","DOI":"10.3390\/nu12051397","article-title":"Potential nutrition and health claims in deastringed\npersimmon fruits (Diospyros kaki L.), variety \u2018Rojo\nBrillante\u2019, PDO \u2019Ribera Del Xu\u0301quer","volume":"12","author":"Domi\u0301nguez Di\u0301az","year":"2020","journal-title":"Nutrients"},{"key":"2026092911091974700_cit4","doi-asserted-by":"publisher","first-page":"914952","DOI":"10.3389\/frfst.2022.914952","article-title":"Valorization of persimmon\nfruit through the development of new food products","volume":"2","author":"Hosseininejad","year":"2022","journal-title":"Front. Food Sci. 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Int."},{"issue":"17","key":"2026092911091974700_cit12","doi-asserted-by":"publisher","first-page":"3847","DOI":"10.3390\/molecules25173847","article-title":"Recent advances\nin supercritical fluid extraction of\nnatural bioactive compounds from natural plant materials","volume":"25","author":"Uwineza","year":"2020","journal-title":"Molecules"},{"issue":"8","key":"2026092911091974700_cit13","doi-asserted-by":"publisher","first-page":"2325","DOI":"10.1039\/C9GC03878G","article-title":"A Review of sustainable and intensified\ntechniques for extraction of food and natural products","volume":"22","author":"Chemat","year":"2020","journal-title":"Green Chem."},{"key":"2026092911091974700_cit14","first-page":"94","article-title":"Supercritical\nfluid extraction of bioactive compounds from plant materials","volume":"146","author":"De Andrade Lima","year":"2019","journal-title":"J. Supercrit. 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Fluids"},{"issue":"7","key":"2026092911091974700_cit17","doi-asserted-by":"publisher","first-page":"1274","DOI":"10.3390\/pr10071274","article-title":"Green chemistry metrics, A review","volume":"10","author":"Marti\u0301nez","year":"2022","journal-title":"Processes"},{"issue":"6","key":"2026092911091974700_cit18","doi-asserted-by":"publisher","first-page":"1645","DOI":"10.1007\/s00216-025-06073-x","article-title":"LC-QTOF-MS\/MS for tentative identification of antioxidant\npeptides in defatted Spirulina platensis cakes obtained by supercritical\nCO2 Extraction","volume":"418","author":"Cuesta Ramos","year":"2025","journal-title":"Anal. Bioanal. 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Technol."},{"issue":"21","key":"2026092911091974700_cit26","doi-asserted-by":"publisher","first-page":"3412","DOI":"10.3390\/foods11213412","article-title":"Towards substitution\nof hexane as extraction solvent of food products and ingredients with\nno regrets","volume":"11","author":"Cravotto","year":"2022","journal-title":"Foods"},{"issue":"1","key":"2026092911091974700_cit27","doi-asserted-by":"publisher","first-page":"136","DOI":"10.1016\/j.foodchem.2005.05.058","article-title":"Sub- and Supercritical\nfluid extraction of functional ingredients from different natural\nsources: Plants, food-by-products, algae and microalgae: A review","volume":"98","author":"Herrero","year":"2006","journal-title":"Food Chem."},{"issue":"10","key":"2026092911091974700_cit28","first-page":"6695","article-title":"Effects of\nsolvent type on phenolics\nand flavonoids content and antioxidant activities in plant extracts","volume":"52","author":"Ghasemzadeh","year":"2015","journal-title":"J. Food Sci. 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Rep."},{"issue":"3","key":"2026092911091974700_cit43","doi-asserted-by":"publisher","first-page":"429","DOI":"10.4014\/jmb.1608.08024","article-title":"Antimicrobial activity of basil,\noregano, and thyme\nessential oils","volume":"27","author":"Sakkas","year":"2017","journal-title":"J. Microbiol. Biotechnol."},{"issue":"6","key":"2026092911091974700_cit44","doi-asserted-by":"publisher","first-page":"1420","DOI":"10.1111\/jam.13602","article-title":"Carvacrol-rich\noregano oil and thymol-rich\nthyme red oil inhibit biofilm formation and the virulence of uropathogenic Escherichia coli","volume":"123","author":"Lee","year":"2017","journal-title":"J. Appl. Microbiol."},{"issue":"12","key":"2026092911091974700_cit45","doi-asserted-by":"publisher","first-page":"1479","DOI":"10.1111\/j.1468-3083.2011.04315.x","article-title":"Antifungal susceptibility\npatterns of yeasts and filamentous fungi isolated from nail infection","volume":"26","author":"Ataides","year":"2012","journal-title":"J. Eur. Acad. Dermatology Venereol"},{"issue":"11","key":"2026092911091974700_cit46","doi-asserted-by":"publisher","first-page":"1898","DOI":"10.3390\/pharmaceutics13111898","article-title":"Carotenoids\nfrom persimmon (Diospyros kaki Thunb.)\nbyproducts exert photoprotective, antioxidative and microbial anti-adhesive\neffects on HaCaT","volume":"13","author":"Gea-Botella","year":"2021","journal-title":"Pharmaceutics"},{"issue":"14","key":"2026092911091974700_cit47","doi-asserted-by":"publisher","first-page":"4954","DOI":"10.1021\/jf0602267","article-title":"Comparison\nof the concentrations\nof phenolic compounds in olive oils and other plant oils: Correlation\nwith antimicrobial activity","volume":"54","author":"Medina","year":"2006","journal-title":"J. Agric. Food\nChem."}],"container-title":["ACS Omega"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsomega.6c05435","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/acsodf\/article-pdf\/11\/38\/57269\/68534655\/acsomega.6c05435.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/acsodf\/article-pdf\/11\/38\/57269\/68534655\/acsomega.6c05435.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T15:50:30Z","timestamp":1790697030000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/acsodf\/article\/11\/38\/57269\/5431466\/Ethanol-Modified-Supercritical-CO2-Extraction-of"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,16]]},"references-count":47,"journal-issue":{"issue":"38","published-online":{"date-parts":[[2026,9,16]]},"published-print":{"date-parts":[[2026,9,29]]}},"URL":"https:\/\/doi.org\/10.1021\/acsomega.6c05435","relation":{},"ISSN":["2470-1343"],"issn-type":[{"value":"2470-1343","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,16]]}}}