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Their structures and configurations were determined by comprehensive spectroscopic analysis including HRMS, NMR, and single-crystal X-ray diffraction. Structurally, all new compounds are classic C20-hetisine-type diterpenoid alkaloids with various oxidation sites. Among them, aconicorines A-D (1\u20134) are unique C20-diterpenoid alkaloids containing a rare sulfonate group. Bioassay results showed that 1, 7, 13\u201317, and 20 dose-dependently inhibited the PD-L1 protein expression at 2.5\u201320 \u03bcM in MDA-MB-231 cells. Furthermore, immunofluorescence staining confirmed the inhibitory effect of 7, 14, 15, and 17 on PD-L1 expression. These findings first demonstrated the effects of C20-diterpenoid alkaloids on PD-L1 expression and suggested that C20-diterpenoid alkaloids are a new type of PD-L1 modulators.<\/jats:p>","DOI":"10.1021\/acsomega.6c05802","type":"journal-article","created":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T15:04:28Z","timestamp":1790089468000},"page":"59532-59546","source":"Crossref","is-referenced-by-count":0,"title":["C-20 Diterpenoid\nAlkaloids with PD-L1 Inhibitory\nActivity from the Roots of\n                    <i>Aconitum coreanum<\/i>"],"prefix":"10.1021","volume":"11","author":[{"given":"Kei-Fong","family":"Ng","sequence":"first","affiliation":[{"name":"Macau University of Science and Technology , , , ,","place":["Taipa, Macau, People\u2019s Republic of China, 999078"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Qian","family":"Li","sequence":"additional","affiliation":[{"name":"Macau University of Science and Technology , , , ,","place":["Taipa, Macau, People\u2019s Republic of China, 999078"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao-Ming","family":"Xiong","sequence":"additional","affiliation":[{"name":"Macau University of Science and Technology , , , ,","place":["Taipa, Macau, People\u2019s Republic of China, 999078"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Chen","sequence":"additional","affiliation":[{"name":"Macau University of Science and Technology , , , ,","place":["Taipa, Macau, People\u2019s Republic of China, 999078"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Chen","sequence":"additional","affiliation":[{"name":"Macau University of Science and Technology , , , ,","place":["Taipa, Macau, People\u2019s Republic of China, 999078"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2806-6883","authenticated-orcid":true,"given":"Li-Ping","family":"Bai","sequence":"additional","affiliation":[{"name":"Macau University of Science and Technology , , , ,","place":["Taipa, Macau, People\u2019s Republic of China, 999078"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1620-6332","authenticated-orcid":true,"given":"Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Macau University of Science and Technology , , , ,","place":["Taipa, Macau, People\u2019s Republic of China, 999078"]},{"name":"Macau University of Science and Technology , , ,","place":["Zhuhai, People\u2019s Republic of China, 519000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ancheng C.","family":"Huang","sequence":"additional","affiliation":[{"name":"Southern University of Science and Technology , , ,","place":["Shenzhen, People\u2019s Republic of China, 518000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7956-2481","authenticated-orcid":true,"given":"Zhi-Hong","family":"Jiang","sequence":"additional","affiliation":[{"name":"Macau University of Science and Technology , , , ,","place":["Taipa, Macau, People\u2019s Republic of China, 999078"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4355-894X","authenticated-orcid":true,"given":"Guo-Yuan","family":"Zhu","sequence":"additional","affiliation":[{"name":"Macau University of Science and Technology , , , ,","place":["Taipa, Macau, People\u2019s Republic of China, 999078"]},{"name":"Macau University of Science and Technology , , ,","place":["Zhuhai, People\u2019s Republic of China, 519000"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,22]]},"reference":[{"issue":"7","key":"2026100610502225800_cit1","doi-asserted-by":"publisher","first-page":"1027","DOI":"10.1084\/jem.192.7.1027","article-title":"Engagement of the PD-1\nimmunoinhibitory receptor by a novel B7 family member leads to negative\nregulation of lymphocyte activation","volume":"192","author":"Freeman","year":"2000","journal-title":"J. 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Macromol."},{"issue":"9","key":"2026100610502225800_cit26","doi-asserted-by":"publisher","first-page":"7085","DOI":"10.1007\/s13277-015-3420-4","article-title":"A Aconitum coreanum\npolysaccharide fraction induces apoptosis of hepatocellular carcinoma\n(HCC) cells via pituitary tumor transforming gene 1 (PTTG1)-mediated\nsuppression of the P13K\/Akt and activation of p38 MAPK signaling pathway\nand displays antitumor activity in vivo","volume":"36","author":"Liang","year":"2015","journal-title":"Tumor\nBiol."},{"issue":"6","key":"2026100610502225800_cit27","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0099697","article-title":"A RG-II\ntype polysaccharide purified from Aconitum coreanum alleviates lipopolysaccharide-induced\ninflammation by inhibiting the NF-\u03baB signal pathway","volume":"9","author":"Li","year":"2014","journal-title":"PLoS One"},{"issue":"5","key":"2026100610502225800_cit28","doi-asserted-by":"publisher","first-page":"876","DOI":"10.5897\/JMPR11.1519","article-title":"Isolation, chemical characterization\nand in vitro antioxidant activities of polysaccharides from Aconitum\ncoreanum","volume":"6","author":"Li","year":"2012","journal-title":"J. Med. Plants Res."},{"key":"2026100610502225800_cit29","first-page":"124","article-title":"Electrophysiological\neffects of Acehytisine Hydrochloride Injection in human body","volume":"29","author":"Zhu","year":"2001","journal-title":"Chin. J Cardiol."},{"issue":"5","key":"2026100610502225800_cit30","doi-asserted-by":"publisher","first-page":"713","DOI":"10.1124\/dmd.114.060905","article-title":"Guanfu base A, an antiarrhythmic\nalkaloid of Aconitum coreanum, Is a CYP2D6 inhibitor of human, monkey,\nand dog isoforms","volume":"43","author":"Sun","year":"2015","journal-title":"Drug Metab. Dispos."},{"issue":"3","key":"2026100610502225800_cit31","doi-asserted-by":"publisher","first-page":"1469","DOI":"10.3892\/or.2017.6165","article-title":"Polysaccharide\nsulphated derivative from Aconitum coreanum induces cell apoptosis\nin the human brain glioblastoma U87MG cell line via the NF-\u03baB\/Bcl-2\ncell apoptotic signaling pathway","volume":"39","author":"Sun","year":"2018","journal-title":"Oncol. Rep."},{"issue":"1","key":"2026100610502225800_cit32","doi-asserted-by":"publisher","first-page":"286","DOI":"10.1021\/jf970585p","article-title":"Antifeedant Delphinium diterpenoid\nalkaloids. Structure\u2013 activity relationships","volume":"46","author":"Gonza\u0301lez-Coloma","year":"1998","journal-title":"J. Agric. Food Chem."},{"issue":"3","key":"2026100610502225800_cit33","doi-asserted-by":"publisher","first-page":"329","DOI":"10.1007\/BF00630253","article-title":"Zeravshanisine\u2500A\nnew alkaloid from Aconitum zeravschanicum","volume":"28","author":"Salimov","year":"1992","journal-title":"Chem.\nNat. Compd."},{"key":"2026100610502225800_cit34","first-page":"1","article-title":"Studies on Aconitum\nspecies in China XVI. New alkaloids in Aconitum coreanum","volume":"12","author":"Liu","year":"1981","journal-title":"Chin. Trad. Herb. Drugs"},{"key":"2026100610502225800_cit35","first-page":"1328","article-title":"Alkaloid constituents from root of\nAconitum coreanum","volume":"35","author":"Yang","year":"2004","journal-title":"Chin. Tradit. Herb. 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