{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T12:13:39Z","timestamp":1788437619009,"version":"build-2803163510"},"reference-count":73,"publisher":"American Chemical Society (ACS)","issue":"34","license":[{"start":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:00:00Z","timestamp":1787011200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior","doi-asserted-by":"publisher","award":["00x0ma614"],"award-info":[{"award-number":["00x0ma614"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005283","name":"Funda??o Cearense de Apoio ao Desenvolvimento Cient?fico e Tecnol?gico","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/501100005283","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Lectins from the genus Erythrina are predominantly galactoside-binding legume lectins with conserved structural features and established use as glycan-recognition tools. However, several species remain incompletely characterized, including the seed lectin from Erythrina fusca (EFusL). Here, EFusL was isolated by single-step lactose-agarose affinity chromatography and characterized by hemagglutination assays, carbohydrate inhibition, physicochemical stability tests, electrophoresis, mass spectrometry-based sequencing, structural modeling, molecular docking, and toxicity assays. EFusL migrated as a 29\u201331 kDa glycoprotein and contained approximately 4.3% neutral carbohydrates. Hemagglutination inhibition confirmed a galactoside-directed profile, with lactose, galactose, and N-acetyl-d-galactosamine among the effective inhibitors. EFusL showed maximal hemagglutinating activity at pH 8.0, retained activity up to 40 \u00b0C, and displayed reduced activity after EDTA treatment with partial recovery following Ca2+\/Mn2+ supplementation. Sequence analysis identified a 249-residue mature lectin highly similar to Erythrina corallodendron and Erythrina cristagalli lectins, including conservation of residues associated with galactoside recognition. Structural modeling supported the canonical \u03b2-sandwich legume lectin fold, and docking analyses indicated that EFusL accommodates galactoside ligands through a conserved polar interaction network comparable to related Erythrina lectins and legume lectins in general. Under the tested conditions, EFusL did not induce lethality in Artemia salina and did not significantly affect NHDF viability, although moderate reductions in HeLa and HT-1080 viability were observed under selected concentration- and time-dependent conditions. Together, these results define EFusL as a structurally conserved, galactoside-binding Erythrina lectin with moderate physicochemical stability and limited cell-type-dependent effects in the evaluated models, supporting its classification within the canonical Erythrina lectin group with similar biotechnological potential.<\/jats:p>","DOI":"10.1021\/acsomega.6c05946","type":"journal-article","created":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T10:02:11Z","timestamp":1787047331000},"page":"51677-51689","source":"Crossref","is-referenced-by-count":0,"title":["Functional and\nStructural Characterization of the\n                    <i>Erythrina fusca<\/i>\n                    Seed Lectin EFusL: A GalNAc-Binding\nLectin with Specificity for Tumor-Associated O-Glycans"],"prefix":"10.1021","volume":"11","author":[{"given":"Messias V.","family":"Oliveira","sequence":"first","affiliation":[{"name":"Federal University of Ceara , , Pici Campus Prof. Prisco Bezerra, S\/N, Pici , ,","place":["Fortaleza, Brazil, 60.455-970"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco W. V.","family":"Martins","sequence":"additional","affiliation":[{"name":"Federal University of Ceara , , Pici Campus Prof. Prisco Bezerra, S\/N, Pici , ,","place":["Fortaleza, Brazil, 60.455-970"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-0446-5275","authenticated-orcid":true,"given":"Clara","family":"Suarez","sequence":"additional","affiliation":[{"name":"Federal University of Ceara , , Pici Campus Prof. Prisco Bezerra, S\/N, Pici , ,","place":["Fortaleza, Brazil, 60.455-970"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valeria M. S.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Federal University of Ceara , , Pici Campus Prof. Prisco Bezerra, S\/N, Pici , ,","place":["Fortaleza, Brazil, 60.455-970"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanir R.","family":"Pinto-Junior","sequence":"additional","affiliation":[{"name":"Federal University of Ceara , , Pici Campus Prof. Prisco Bezerra, S\/N, Pici , ,","place":["Fortaleza, Brazil, 60.455-970"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5730-8829","authenticated-orcid":true,"given":"Vinicius J. S.","family":"Osterne","sequence":"additional","affiliation":[{"name":"Federal University of Ceara , , Pici Campus Prof. Prisco Bezerra, S\/N, Pici , ,","place":["Fortaleza, Brazil, 60.455-970"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1589-7717","authenticated-orcid":true,"given":"Luiz A. G.","family":"Souza","sequence":"additional","affiliation":[{"name":"National Institute for Research in the Amazon , , 2936 Andre Araujo Avenue , , ,","place":["Petropolis, Manaus, Brazil, 69067-375"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Els J. M.","family":"Van Damme","sequence":"additional","affiliation":[{"name":"Ghent University , , Proeftuinstraat 86 , ,","place":["Ghent, Belgium, 9000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo B.","family":"Leal","sequence":"additional","affiliation":[{"name":"Federal University of Santa Catarina , , Block G, 2nd Floor, Room 219 , ,","place":["Florianopolis, Brazil, 88037-000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5791-6170","authenticated-orcid":true,"given":"Benildo S.","family":"Cavada","sequence":"additional","affiliation":[{"name":"Federal University of Ceara , , Pici Campus Prof. Prisco Bezerra, S\/N, Pici , ,","place":["Fortaleza, Brazil, 60.455-970"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2220-4552","authenticated-orcid":true,"given":"Kyria S.","family":"Nascimento","sequence":"additional","affiliation":[{"name":"Federal University of Ceara , , Pici Campus Prof. Prisco Bezerra, S\/N, Pici , ,","place":["Fortaleza, Brazil, 60.455-970"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,18]]},"reference":[{"key":"2026090307200856500_cit1","doi-asserted-by":"publisher","first-page":"111096","DOI":"10.1016\/j.plantsci.2021.111096","article-title":"Review: The Multiple Roles of Plant\nLectins","volume":"313","author":"De Coninck","year":"2021","journal-title":"Plant Sci."},{"issue":"4","key":"2026090307200856500_cit2","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1007\/s12038-021-00225-8","article-title":"Research Advances and\nProspects of Legume Lectins","volume":"46","author":"Katoch","year":"2021","journal-title":"J. Biosci."},{"key":"2026090307200856500_cit3","doi-asserted-by":"publisher","first-page":"109241","DOI":"10.1016\/j.carres.2024.109241","article-title":"Revisiting Legume Lectins: Structural\nOrganization and Carbohydrate-Binding Properties","volume":"544","author":"Osterne","year":"2024","journal-title":"Carbohydr. Res."},{"issue":"1","key":"2026090307200856500_cit4","doi-asserted-by":"publisher","first-page":"3528","DOI":"10.1038\/s41467-026-70188-7","article-title":"Altering the\nCarbohydrate-Binding Specificity of the Legume Lectin FRIL through\nStructure-Guided Engineering","volume":"17","author":"Liu","year":"2026","journal-title":"Nat. Commun."},{"issue":"2","key":"2026090307200856500_cit5","doi-asserted-by":"publisher","first-page":"247","DOI":"10.1111\/j.1432-1033.1982.tb19760.x","article-title":"Purification\nand Properties of a D-galactose\/N-Acetyl-D-Galactosamine-Specific\nLectin from Erythrina Cristagalli","volume":"123","author":"Iglesias","year":"1982","journal-title":"Eur. J. Biochem."},{"issue":"20","key":"2026090307200856500_cit6","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e39471","article-title":"From Inflammation to Immune Regulation: The Dual Nature\nof Dietary Lectins in Health and Disease","volume":"10","author":"Konozy","year":"2024","journal-title":"Heliyon"},{"issue":"1","key":"2026090307200856500_cit7","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1177\/172460089801300105","article-title":"Erythrina\nCristagalli Lectin-Reactive \u03b1-Fetoprotein-E2: A Marker of Hepatocellular\nCarcinoma and Other Malignancies","volume":"13","author":"Kamei","year":"1998","journal-title":"Int. J. Biol.\nMarkers"},{"issue":"5","key":"2026090307200856500_cit8","doi-asserted-by":"publisher","first-page":"707","DOI":"10.1089\/scd.2012.0365","article-title":"Lectin from\nErythrina Cristagalli Supports Undifferentiated Growth and Differentiation\nof Human Pluripotent Stem Cells","volume":"22","author":"Mikkola","year":"2013","journal-title":"Stem Cells\nDev."},{"issue":"4","key":"2026090307200856500_cit9","doi-asserted-by":"publisher","first-page":"917","DOI":"10.1006\/jmbi.1998.1664","article-title":"Structures of the Erythrina Corallodendron Lectin and\nof Its Complexes with Mono- and Disaccharides","volume":"277","author":"Elgavish","year":"1998","journal-title":"J. Mol. Biol."},{"issue":"1","key":"2026090307200856500_cit10","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1016\/S0022-2836(02)00554-5","article-title":"High-Resolution Crystal\nStructures of Erythrina Cristagalli Lectin in Complex with Lactose\nand 2\u2019-Alpha-L-Fucosyllactose and Correlation with Thermodynamic\nBinding Data","volume":"321","author":"Svensson","year":"2002","journal-title":"J. Mol. Biol."},{"issue":"12","key":"2026090307200856500_cit11","doi-asserted-by":"publisher","first-page":"2803","DOI":"10.1016\/0031-9422(85)80004-2","article-title":"Isolation\nand Properties of N-Acetyllactosamine-Specific\nLectins from Nine Erythrina Species","volume":"24","author":"Lis","year":"1985","journal-title":"Phytochemistry"},{"key":"2026090307200856500_cit12","unstructured":"Erythrina fusca\nLour. Plants of the World Online; https:\/\/powo.science.kew.org\/taxon\/urn%3Alsid%3Aipni.org%3Anames%3A494431-1. accessed 23 February 2026."},{"issue":"2","key":"2026090307200856500_cit13","doi-asserted-by":"publisher","first-page":"224","DOI":"10.1007\/s42965-024-00335-y","article-title":"Periodic\nFlooding and Edaphic Factors Shape Erythrina Fusca Dominance in Riparian\nForests in the Pantanal Wetland","volume":"65","author":"Gris","year":"2024","journal-title":"Trop. Ecol."},{"key":"2026090307200856500_cit14","doi-asserted-by":"publisher","first-page":"821","DOI":"10.1016\/s1572-5995(05)80070-5","article-title":"Bioactive Non-Alkaloidal\nConstituents from the Genus Erythrina","author":"Majinda","year":"2005","journal-title":"Stud.\nNat. Prod. Chem."},{"key":"2026090307200856500_cit15","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1007\/978-1-4939-1292-6_4","article-title":"Hemagglutination (inhibition)\nAssay","volume":"1200","author":"Sano","year":"2014","journal-title":"Methods\nMol. Biol."},{"issue":"5259","key":"2026090307200856500_cit16","doi-asserted-by":"publisher","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage\nof Structural Proteins during the Assembly of the Head of Bacteriophage\nT4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"issue":"1","key":"2026090307200856500_cit17","doi-asserted-by":"publisher","first-page":"148","DOI":"10.1016\/0003-2697(69)90383-2","article-title":"Glycoprotein Staining\nFollowing Electrophoresis on Acrylamide Gels","volume":"30","author":"Zacharius","year":"1969","journal-title":"Anal. Biochem."},{"issue":"3","key":"2026090307200856500_cit18","doi-asserted-by":"publisher","first-page":"350","DOI":"10.1021\/ac60111a017","article-title":"Colorimetric\nMethod for Determination of Sugars and Related Substances","volume":"28","author":"DuBois","year":"1956","journal-title":"Anal. Chem."},{"issue":"1","key":"2026090307200856500_cit19","doi-asserted-by":"publisher","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A Rapid\nand Sensitive Method for the Quantitation of Microgram Quantities\nof Protein Utilizing the Principle of Protein-Dye Binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"issue":"11","key":"2026090307200856500_cit20","doi-asserted-by":"publisher","first-page":"527","DOI":"10.1002\/jmr.2200","article-title":"An Overview\nof Lectins Purification Strategies","volume":"25","author":"Nascimento","year":"2012","journal-title":"J. Mol.\nRecognit."},{"issue":"6","key":"2026090307200856500_cit21","doi-asserted-by":"publisher","first-page":"2856","DOI":"10.1038\/nprot.2006.468","article-title":"In-Gel Digestion\nfor Mass Spectrometric Characterization of Proteins and Proteomes","volume":"1","author":"Shevchenko","year":"2006","journal-title":"Nat. Protoc."},{"issue":"D1","key":"2026090307200856500_cit22","doi-asserted-by":"publisher","first-page":"D543","DOI":"10.1093\/nar\/gkae1011","article-title":"The PRIDE\nDatabase at 20 Years: 2025 Update","volume":"53","author":"Perez-Riverol","year":"2025","journal-title":"Nucleic Acids\nRes."},{"issue":"1","key":"2026090307200856500_cit23","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1186\/1471-2105-13-134","article-title":"Primer-BLAST: A Tool to Design Target-Specific\nPrimers for Polymerase Chain Reaction","volume":"13","author":"Ye","year":"2012","journal-title":"BMC Bioinf."},{"key":"2026090307200856500_cit24","unstructured":"Doyle, J. J.; Doyle, J. L.\n          A Rapid DNA Isolation\nProcedure for Small Quantities of Fresh Leaf Tissue; 1987. https:\/\/worldveg.tind.io\/record\/33886\/."},{"issue":"62","key":"2026090307200856500_cit25","doi-asserted-by":"publisher","first-page":"3923","DOI":"10.3791\/3923","article-title":"Agarose Gel Electrophoresis\nfor the Separation of DNA Fragments","volume":"20","author":"Lee","year":"2012","journal-title":"J. Vis.\nExp."},{"issue":"12","key":"2026090307200856500_cit26","doi-asserted-by":"publisher","first-page":"5463","DOI":"10.1073\/pnas.74.12.5463","article-title":"DNA Sequencing\nwith Chain-Terminating\nInhibitors","volume":"74","author":"Sanger","year":"1977","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"W1","key":"2026090307200856500_cit27","doi-asserted-by":"publisher","first-page":"W276","DOI":"10.1093\/nar\/gkac240","article-title":"Search and Sequence Analysis Tools Services from EMBL-EBI in 2022","volume":"50","author":"Madeira","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"2026090307200856500_cit28","doi-asserted-by":"publisher","first-page":"W320","DOI":"10.1093\/nar\/gku316","article-title":"Deciphering Key Features\nin Protein Structures with the New ENDscript\nServer","volume":"42","author":"Robert","year":"2014","journal-title":"Nucleic Acids Res."},{"issue":"W1","key":"2026090307200856500_cit29","doi-asserted-by":"publisher","first-page":"W402","DOI":"10.1093\/nar\/gkz297","article-title":"The PSIPRED Protein Analysis Workbench: 20 Years on","volume":"47","author":"Buchan","year":"2019","journal-title":"Nucleic Acids Res."},{"issue":"2","key":"2026090307200856500_cit30","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1006\/jmbi.1999.3091","article-title":"Protein\nSecondary Structure Prediction Based on Position-Specific Scoring\nMatrices","volume":"292","author":"Jones","year":"1999","journal-title":"J. Mol. Biol."},{"issue":"7873","key":"2026090307200856500_cit31","doi-asserted-by":"publisher","first-page":"583","DOI":"10.1038\/s41586-021-03819-2","article-title":"Highly Accurate Protein\nStructure Prediction with AlphaFold","volume":"596","author":"Jumper","year":"2021","journal-title":"Nature"},{"issue":"7873","key":"2026090307200856500_cit32","doi-asserted-by":"publisher","first-page":"583","DOI":"10.1038\/s41586-021-03819-2","article-title":"Highly Accurate Protein\nStructure Prediction with AlphaFold","volume":"596","author":"Jumper","year":"2021","journal-title":"Nature"},{"issue":"9","key":"2026090307200856500_cit33","doi-asserted-by":"publisher","first-page":"1511","DOI":"10.1002\/pro.5560020916","article-title":"Verification of Protein Structures: Patterns of Nonbonded\nAtomic Interactions","volume":"2","author":"Colovos","year":"1993","journal-title":"Protein Sci."},{"key":"2026090307200856500_cit34","doi-asserted-by":"publisher","first-page":"396","DOI":"10.1016\/S0076-6879(97)77022-8","article-title":"VERIFY3D: Assessment of Protein Models\nwith Three-Dimensional Profiles","volume":"277","author":"Eisenberg","year":"1997","journal-title":"Methods Enzymol."},{"issue":"2","key":"2026090307200856500_cit35","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1107\/S0021889892009944","article-title":"PROCHECK: A Program\nto Check the Stereochemical Quality of Protein Structures","volume":"26","author":"Laskowski","year":"1993","journal-title":"J. Appl. Crystallogr."},{"issue":"8016","key":"2026090307200856500_cit36","doi-asserted-by":"publisher","first-page":"493","DOI":"10.1038\/s41586-024-07487-w","article-title":"Accurate Structure\nPrediction of\nBiomolecular Interactions with AlphaFold 3","volume":"630","author":"Abramson","year":"2024","journal-title":"Nature"},{"issue":"1","key":"2026090307200856500_cit37","doi-asserted-by":"publisher","first-page":"7296","DOI":"10.1038\/s41467-024-51801-z","article-title":"AlphaFold Predictions\nof Fold-Switched Conformations\nAre Driven by Structure Memorization","volume":"15","author":"Chakravarty","year":"2024","journal-title":"Nat. Commun."},{"issue":"3","key":"2026090307200856500_cit38","doi-asserted-by":"publisher","first-page":"727","DOI":"10.1006\/jmbi.1996.0897","article-title":"Development\nand Validation of a Genetic Algorithm for Flexible Docking","volume":"267","author":"Jones","year":"1997","journal-title":"J. Mol. Biol."},{"issue":"6","key":"2026090307200856500_cit39","doi-asserted-by":"publisher","first-page":"737","DOI":"10.1007\/s10822-012-9551-4","article-title":"Pose Prediction\nand Virtual Screening\nPerformance of GOLD Scoring Functions in a Standardized Test","volume":"26","author":"Liebeschuetz","year":"2012","journal-title":"J. Comput. Aided Mol. Des."},{"issue":"11","key":"2026090307200856500_cit40","doi-asserted-by":"publisher","first-page":"935","DOI":"10.1038\/nrd1549","article-title":"Docking and Scoring\nin Virtual Screening for Drug Discovery: Methods and Applications","volume":"3","author":"Kitchen","year":"2004","journal-title":"Nat. Rev. Drug Discovery"},{"issue":"9","key":"2026090307200856500_cit41","doi-asserted-by":"publisher","first-page":"1889","DOI":"10.1021\/acs.jcim.8b00120","article-title":"Defining the Specificity\nof Carbohydrate-Protein Interactions\nby Quantifying Functional Group Contributions","volume":"58","author":"Sood","year":"2018","journal-title":"J. Chem. Inf. Model."},{"issue":"4","key":"2026090307200856500_cit42","doi-asserted-by":"publisher","first-page":"753","DOI":"10.1110\/ps.44001","article-title":"Chemical Characteristics\nof Dimer Interfaces in the\nLegume Lectin Family","volume":"10","author":"Elgavish","year":"2001","journal-title":"Protein Sci."},{"issue":"W1","key":"2026090307200856500_cit43","doi-asserted-by":"publisher","first-page":"W463","DOI":"10.1093\/nar\/gkaf361","article-title":"PLIP 2025:\nIntroducing Protein-Protein Interactions to the Protein-Ligand Interaction\nProfiler","volume":"53","author":"Schake","year":"2025","journal-title":"Nucleic Acids Res."},{"issue":"9","key":"2026090307200856500_cit44","doi-asserted-by":"publisher","first-page":"540","DOI":"10.1021\/ml100164p","article-title":"Drawing the PDB: Protein-Ligand\nComplexes in Two Dimensions","volume":"1","author":"Stierand","year":"2010","journal-title":"ACS Med. Chem.\nLett."},{"issue":"6","key":"2026090307200856500_cit45","doi-asserted-by":"publisher","first-page":"853","DOI":"10.1002\/cmdc.200700010","article-title":"From Modeling to Medicinal Chemistry: Automatic Generation of Two-Dimensional\nComplex Diagrams","volume":"2","author":"Stierand","year":"2007","journal-title":"ChemMedchem"},{"issue":"14","key":"2026090307200856500_cit46","doi-asserted-by":"publisher","first-page":"1710","DOI":"10.1093\/bioinformatics\/btl150","article-title":"Molecular\nComplexes at a Glance:\nAutomated Generation of Two-Dimensional Complex Diagrams","volume":"22","author":"Stierand","year":"2006","journal-title":"Bioinformatics"},{"issue":"3","key":"2026090307200856500_cit47","doi-asserted-by":"publisher","first-page":"1065","DOI":"10.1021\/ci049958u","article-title":"Automated Drawing of Structural Molecular\nFormulas under Constraints","volume":"44","author":"Fricker","year":"2004","journal-title":"J. Chem. Inf. Comput.\nSci."},{"key":"2026090307200856500_cit48","doi-asserted-by":"publisher","first-page":"845","DOI":"10.1016\/j.ijbiomac.2019.10.102","article-title":"Purification\nand Partial Characterization of a New Lectin from Parkia Panurensis\nBenth. Ex H.C. Hopkins Seeds (Leguminosae Family; Mimosoideae Subfamily)\nand Evaluation of Its Biological Effects","volume":"145","author":"Cavada","year":"2020","journal-title":"Int.\nJ. Biol. Macromol."},{"issue":"5","key":"2026090307200856500_cit49","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1055\/s-2007-971236","article-title":"Brine Shrimp:\nA Convenient General Bioassay for Active\nPlant Constituents","volume":"45","author":"Meyer","year":"1982","journal-title":"Planta Med."},{"issue":"3","key":"2026090307200856500_cit50","doi-asserted-by":"publisher","first-page":"382","DOI":"10.1016\/0147-6513(81)90012-9","article-title":"Proposal for\na Short-Term Toxicity Test with Artemia Nauplii","volume":"5","author":"Vanhaecke","year":"1981","journal-title":"Ecotoxicol. Environ. 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