{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T12:13:10Z","timestamp":1788437590208,"version":"build-2803163510"},"reference-count":61,"publisher":"American Chemical Society (ACS)","issue":"34","license":[{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/na","name":"SiciliAn MicronanOTecH Research And Innovation CEnter","doi-asserted-by":"publisher","award":["ECS_00000022"],"award-info":[{"award-number":["ECS_00000022"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The development of robust and recyclable enzyme-based materials is essential for advancing sustainable biocatalysis and biotechnologies. Herein, we report the design of covalent enzyme-halloysite biohybrid materials obtained by the controlled functionalization of halloysite nanotubes (HNTs), a naturally abundant, low-cost, and biodegradable clay nanomaterial acting as a dynamic biological interface. Two structurally and functionally distinct enzymes, collagenase G (ColG) and laccase (Lac), were selected as model systems to investigate how the halloysite surface can modulate the enzyme organization and catalytic behavior. Through tailored interfacial functionalization of the HNT external surface, stable enzyme-clay biointerfaces were obtained while preserving enzymatic activity. Comprehensive spectroscopic, thermal, and microscopic analyses confirmed the successful formation of homogeneous hybrid biointerfaces and revealed distinct supramolecular organization, depending on the immobilized enzyme. Enzymatic assays demonstrate that both HNTs-ColG and HNTs-Lac retained their catalytic function after surface conjugation. Notably, the laccase-based biomaterial efficiently degrades methyl orange under mediator-free conditions, a behavior rarely observed in halloysite-supported laccase systems, while operating under both batch and continuous flow conditions and exhibiting high recyclability over multiple cycles. These findings suggest that halloysite nanotubes do not merely behave as a passive support but rather as adaptive inorganic-biological interfaces capable of dynamically influencing enzyme function through interfacial effects, in particular, via the covalent-induced conformational modulation of the enzyme after its grafting onto the external HNTs surface. The proposed strategy opens new perspectives for the development of functional enzyme materials for bioremediation and biomedical applications.<\/jats:p>","DOI":"10.1021\/acsomega.6c06020","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T16:02:11Z","timestamp":1786982531000},"page":"51690-51700","source":"Crossref","is-referenced-by-count":0,"title":["Halloysite Nanotubes\nas Dynamic Interfaces for Functional\nEnzyme Biomaterials"],"prefix":"10.1021","volume":"11","author":[{"given":"Giuseppe","family":"Cina\u0300","sequence":"first","affiliation":[{"name":"Universita\u0300 di Catania , , Viale Andrea Doria 6 , , ,","place":["Catania, Italy, 95125"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Massaro","sequence":"additional","affiliation":[{"name":"Universita\u0300 degli Studi di Palermo , , Viale delle Scienze, Parco d\u2019Orleans II, Ed. 17 , ,","place":["Palermo, Italy, 90128"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federica","family":"Leone","sequence":"additional","affiliation":[{"name":"Universita\u0300 di Catania , , Viale Andrea Doria 6 , , ,","place":["Catania, Italy, 95125"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Sciacca","sequence":"additional","affiliation":[{"name":"Universita\u0300 di Catania , , Viale Andrea Doria 6 , , ,","place":["Catania, Italy, 95125"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nunzio","family":"Cardullo","sequence":"additional","affiliation":[{"name":"Universita\u0300 di Catania , , Viale Andrea Doria 6 , , ,","place":["Catania, Italy, 95125"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6212-5154","authenticated-orcid":true,"given":"Vera","family":"Muccilli","sequence":"additional","affiliation":[{"name":"Universita\u0300 di Catania , , Viale Andrea Doria 6 , , ,","place":["Catania, Italy, 95125"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0802-7358","authenticated-orcid":true,"given":"Angelo","family":"Nicosia","sequence":"additional","affiliation":[{"name":"Universita\u0300 di Catania , , Viale Andrea Doria 6 , , ,","place":["Catania, Italy, 95125"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Placido G.","family":"Mineo","sequence":"additional","affiliation":[{"name":"Universita\u0300 di Catania , , Viale Andrea Doria 6 , , ,","place":["Catania, Italy, 95125"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rita","family":"Sa\u0301nchez-Espejo","sequence":"additional","affiliation":[{"name":"University of Granada , , , ,","place":["Granada, Spain, 18071"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ce\u0301sar","family":"Viseras","sequence":"additional","affiliation":[{"name":"University of Granada , , , ,","place":["Granada, Spain, 18071"]},{"name":"CSIC-UGR , , , ,","place":["Armilla, Granada, Spain, 18100"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8705-8763","authenticated-orcid":true,"given":"Serena","family":"Riela","sequence":"additional","affiliation":[{"name":"Universita\u0300 di Catania , , Viale Andrea Doria 6 , , ,","place":["Catania, Italy, 95125"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,17]]},"reference":[{"key":"2026090307181006300_cit1","doi-asserted-by":"publisher","first-page":"1242","DOI":"10.36347\/sajb.2025.v13i08.017","article-title":"Green Biochemistry\nand Sustainable Biocatalysis Toward Eco-Friendly Industrial Innovation","volume":"13","author":"Aslam","year":"2025","journal-title":"Sch Acad. J. Biosci"},{"issue":"4","key":"2026090307181006300_cit2","doi-asserted-by":"publisher","first-page":"2307","DOI":"10.1021\/acs.chemrev.5b00472","article-title":"Enzymes as\nGreen Catalysts for Precision Macromolecular Synthesis","volume":"116","author":"Shoda","year":"2016","journal-title":"Chem. Rev."},{"issue":"18","key":"2026090307181006300_cit3","doi-asserted-by":"publisher","first-page":"7041","DOI":"10.1039\/D3GC01878D","article-title":"From green\nto circular chemistry\npaved by biocatalysis","volume":"25","author":"Lozano","year":"2023","journal-title":"Green Chem."},{"issue":"4","key":"2026090307181006300_cit4","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1007\/s12154-013-0102-9","article-title":"Enzyme immobilization:\nan update","volume":"6","author":"Homaei","year":"2013","journal-title":"J. Chem. Biol."},{"issue":"3","key":"2026090307181006300_cit5","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1042\/bio_2022_109","article-title":"Back to the future:\ntaking enzymes to the next level of sustainability","volume":"44","author":"Padrosa","year":"2022","journal-title":"Biochemist"},{"key":"2026090307181006300_cit6","doi-asserted-by":"publisher","DOI":"10.1016\/j.cej.2024.158054","article-title":"Covalent immobilization: A review\nfrom an enzyme perspective","volume":"503","author":"Prabhakar","year":"2025","journal-title":"Chemical Engineering\nJournal"},{"issue":"9","key":"2026090307181006300_cit7","doi-asserted-by":"publisher","first-page":"969","DOI":"10.3390\/min15090969","article-title":"Clay Minerals as Enzyme Carriers for Pollutant Removal\nfrom Wastewater: A Comprehensive Review","volume":"15","author":"Sayahi","year":"2025","journal-title":"Minerals"},{"key":"2026090307181006300_cit8","doi-asserted-by":"publisher","DOI":"10.1016\/j.cogsc.2022.100593","article-title":"Enzyme immobilization\nstrategies for the design of robust and efficient biocatalysts","volume":"35","author":"Guisan","year":"2022","journal-title":"Current Opinion in Green and Sustainable Chemistry"},{"issue":"13","key":"2026090307181006300_cit9","doi-asserted-by":"publisher","first-page":"2343","DOI":"10.1021\/ac960930u","article-title":"Characterization\nof Immobilization of an Enzyme in\na Modified Y Zeolite Matrix and Its Application to an Amperometric\nGlucose Biosensor","volume":"69","author":"Liu","year":"1997","journal-title":"Anal. Chem."},{"issue":"7","key":"2026090307181006300_cit10","doi-asserted-by":"publisher","first-page":"1857","DOI":"10.1021\/acs.oprd.1c00404","article-title":"Recent Advances\nin Enzyme Immobilization Utilizing\nNanotechnology for Biocatalysis","volume":"26","author":"T.sriwong","year":"2022","journal-title":"Org. Process\nRes. Dev."},{"issue":"12","key":"2026090307181006300_cit11","doi-asserted-by":"publisher","first-page":"488","DOI":"10.3390\/jcs7120488","article-title":"b., Recent Advances in Enzyme Immobilisation\nStrategies: An Overview\nof Techniques and Composite Carriers","volume":"7","author":"Mohidem","year":"2023","journal-title":"Journal\nof Composites Science"},{"key":"2026090307181006300_cit12","doi-asserted-by":"publisher","first-page":"1779","DOI":"10.1016\/j.jcis.2021.08.155","article-title":"Site-specific\nhalloysite functionalization by polydopamine: A new synthetic route\nfor potential near infrared-activated delivery system","volume":"606","author":"Alfieri","year":"2022","journal-title":"J. Colloid Interface Sci."},{"key":"2026090307181006300_cit13","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1016\/j.jcis.2022.03.151","article-title":"Nanocarrier based on halloysite and\nfluorescent probe for intracellular delivery of peptide nucleic acids","volume":"620","author":"Massaro","year":"2022","journal-title":"J. Colloid Interface Sci."},{"issue":"28","key":"2026090307181006300_cit14","doi-asserted-by":"publisher","first-page":"6685","DOI":"10.1039\/D3TB00637A","article-title":"Exploiting the interaction between halloysite and charged\nPNAs for their controlled release","volume":"11","author":"Riela","year":"2023","journal-title":"J. Mater.\nChem. B"},{"key":"2026090307181006300_cit15","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1016\/j.jcis.2024.02.136","article-title":"Carrier capability\nof halloysite nanotubes for the intracellular delivery of antisense\nPNA targeting mRNA of neuroglobin gene","volume":"663","author":"Falanga","year":"2024","journal-title":"J. Colloid\nInterface Sci."},{"key":"2026090307181006300_cit16","doi-asserted-by":"publisher","first-page":"236","DOI":"10.1016\/j.jcis.2019.05.062","article-title":"Halloysite nanotubes-carbon dots\nhybrids multifunctional nanocarrier with positive cell target ability\nas a potential non-viral vector for oral gene therapy","volume":"552","author":"Massaro","year":"2019","journal-title":"J. Colloid Interface Sci."},{"issue":"2","key":"2026090307181006300_cit17","doi-asserted-by":"publisher","first-page":"615","DOI":"10.1021\/acs.biomac.5b01542","article-title":"Halloysite\nClay Nanotubes for Enzyme\nImmobilization","volume":"17","author":"Tully","year":"2016","journal-title":"Biomacromolecules"},{"issue":"21","key":"2026090307181006300_cit18","doi-asserted-by":"publisher","first-page":"10559","DOI":"10.1021\/am4022973","article-title":"Surface Modification of Halloysite\nNanotubes with Dopamine for Enzyme Immobilization","volume":"5","author":"Chao","year":"2013","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"2026090307181006300_cit19","doi-asserted-by":"publisher","first-page":"156","DOI":"10.1016\/j.jcis.2018.04.025","article-title":"Halloysite nanotubes\nfor efficient loading, stabilization and controlled\nrelease of insulin","volume":"524","author":"Massaro","year":"2018","journal-title":"J. Colloid Interface Sci."},{"key":"2026090307181006300_cit20","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/B978-0-443-15912-1.00006-9","article-title":"Halloysite nanotubes\nas support material for enzyme immobilization","volume-title":"Smart Halloysite Nanotubes","author":"Sa\u0301nchez-Ferna\u0301ndez","year":"2026"},{"key":"2026090307181006300_cit21","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2023.113511","article-title":"Nanoformulations based on collagenases loaded into halloysite\/Veegum\u00ae\nclay minerals for potential pharmaceutical applications","volume":"230","author":"Massaro","year":"2023","journal-title":"Colloids Surf., B"},{"issue":"18","key":"2026090307181006300_cit22","doi-asserted-by":"publisher","first-page":"15492","DOI":"10.1021\/acsami.7b02531","article-title":"Supermagnetically\ntuned halloysite\nnanotubes functionalized with aminosilane for covalent laccase immobilization","volume":"9","author":"Kadam","year":"2017","journal-title":"ACS Appl. Mater. Interfaces"},{"issue":"10","key":"2026090307181006300_cit23","doi-asserted-by":"publisher","first-page":"5370","DOI":"10.3390\/ijms25105370","article-title":"Comparison of Synthetic Pathways\nfor Obtaining Fluorescent Nanomaterials Based on Halloysite and Carbon\nDots for Potential Biological Sensing","volume":"25","author":"Massaro","year":"2024","journal-title":"International\nJournal of Molecular Sciences"},{"issue":"24","key":"2026090307181006300_cit24","doi-asserted-by":"publisher","first-page":"12775","DOI":"10.1021\/acsanm.5c02027","article-title":"Halloysite Nanotubes Functionalized\nwith Naphthalene Diimide and Peptide Nucleic Acid Derivatives: Toward\nMultifunctional Nanomaterials","volume":"8","author":"Cardano","year":"2025","journal-title":"ACS Applied Nano\nMaterials"},{"issue":"1","key":"2026090307181006300_cit25","doi-asserted-by":"publisher","first-page":"2595","DOI":"10.1515\/ntrev-2022-0148","article-title":"Recent developments and applications of smart nanoparticles\nin biomedicine","volume":"11","author":"Campora","year":"2022","journal-title":"Nanotechnol. Rev."},{"issue":"43","key":"2026090307181006300_cit26","doi-asserted-by":"publisher","first-page":"24075","DOI":"10.1021\/acsami.5b07116","article-title":"Hybrid Collagenase Nanocapsules for\nEnhanced Nanocarrier Penetration in Tumoral Tissues","volume":"7","author":"Villegas","year":"2015","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"2026090307181006300_cit27","doi-asserted-by":"publisher","DOI":"10.1016\/j.cej.2025.167154","article-title":"Entrapment of laccase-halloysite\nnanotubes in chitosan beads for pharmaceutical degradation in wastewater","volume":"521","author":"Sa\u0301","year":"2025","journal-title":"Chemical Engineering Journal"},{"issue":"2","key":"2026090307181006300_cit28","doi-asserted-by":"publisher","first-page":"644","DOI":"10.26554\/sti.2025.10.2.644-659","article-title":"Decolorization of Synthetic Dyes by Laccase of Phellinus\nnoxius BRB\n11 Immobilized with Halloysite Nanotubes","volume":"10","author":"Naser","year":"2025","journal-title":"Science\nand Technology Indonesia"},{"key":"2026090307181006300_cit29","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2023.120999","article-title":"Laccase:\nA potential biocatalyst for pollutant degradation","volume":"319","author":"Dong","year":"2023","journal-title":"Environ. Pollut."},{"issue":"8","key":"2026090307181006300_cit30","doi-asserted-by":"publisher","first-page":"1282","DOI":"10.1080\/10643389.2020.1854565","article-title":"Laccase immobilization\nwith metal-organic frameworks: Current status, remaining challenges\nand future perspectives","volume":"52","author":"Huang","year":"2022","journal-title":"Critical Reviews in\nEnvironmental Science and Technology"},{"issue":"12","key":"2026090307181006300_cit31","doi-asserted-by":"publisher","DOI":"10.1016\/j.arabjc.2022.104305","article-title":"The biobleaching potential of laccase\nproduced from mandarin peelings: Impetus for a circular bio-based\neconomy in textile biofinishing","volume":"15","author":"Unuofin","year":"2022","journal-title":"Arabian Journal\nof Chemistry"},{"issue":"10","key":"2026090307181006300_cit32","doi-asserted-by":"publisher","DOI":"10.1016\/j.jksus.2023.102948","article-title":"Biodegradation\nof chromium by laccase action of Ganoderma multipileum","volume":"35","author":"Alshiekheid","year":"2023","journal-title":"Journal of King Saud University - Science"},{"key":"2026090307181006300_cit33","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2023.124968","article-title":"Structural insights,\nbiocatalytic characteristics, and application prospects of lignin-modifying\nenzymes for sustainable biotechnology","volume":"242","author":"Singh","year":"2023","journal-title":"Int.\nJ. Biol. Macromol."},{"issue":"6","key":"2026090307181006300_cit34","doi-asserted-by":"publisher","first-page":"614","DOI":"10.1039\/D1CB00259G","article-title":"Laccase-mediated\nsynthesis of bioactive\nnatural products and their analogues","volume":"3","author":"Cardullo","year":"2022","journal-title":"RSC Chemical\nBiology"},{"key":"2026090307181006300_cit35","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2024.134375","article-title":"Development of alginate film filled\nwith halloysite-carbon dots for active food packaging","volume":"277","author":"Cina\u0300","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"issue":"28","key":"2026090307181006300_cit36","doi-asserted-by":"publisher","first-page":"20184","DOI":"10.1074\/jbc.M112.448548","article-title":"Structural Basis for Activity Regulation\nand Substrate Preference of Clostridial Collagenases G, H, and T *","volume":"288","author":"Eckhard","year":"2013","journal-title":"J. Biol. Chem."},{"issue":"8","key":"2026090307181006300_cit37","doi-asserted-by":"publisher","first-page":"1143","DOI":"10.3390\/pharmaceutics13081143","article-title":"Collagenase\nActivity of Bromelain Immobilized at Gold Nanoparticle Interfaces\nfor Therapeutic Applications","volume":"13","author":"Brito","year":"2021","journal-title":"Pharmaceutics"},{"key":"2026090307181006300_cit38","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2024.133114","article-title":"Immobilization\nof collagenase in inorganic hybrid nanoflowers with enhanced stability,\nproteolytic activity, and their anti-amyloid potential","volume":"274","author":"Jamal","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"issue":"40","key":"2026090307181006300_cit39","doi-asserted-by":"publisher","first-page":"15742","DOI":"10.1021\/jp805657t","article-title":"Functionalization\nof Halloysite Clay\nNanotubes by Grafting with \u03b3-Aminopropyltriethoxysilane","volume":"112","author":"Yuan","year":"2008","journal-title":"J. Phys. Chem. C"},{"issue":"27","key":"2026090307181006300_cit40","doi-asserted-by":"publisher","first-page":"7377","DOI":"10.1039\/C8TC01424H","article-title":"Photoluminescent\nhybrid nanomaterials\nfrom modified halloysite nanotubes","volume":"6","author":"Massaro","year":"2018","journal-title":"Journal\nof Materials Chemistry C"},{"key":"2026090307181006300_cit41","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1016\/j.jcis.2024.02.136","article-title":"Carrier capability\nof halloysite nanotubes for the intracellular delivery of antisense\nPNA targeting mRNA of neuroglobin gene","volume":"663","author":"Falanga","year":"2024","journal-title":"J. Colloid\nInterface Sci."},{"issue":"1","key":"2026090307181006300_cit42","doi-asserted-by":"publisher","DOI":"10.1088\/1757-899X\/627\/1\/012025","article-title":"Laccase\nEnzyme Immobilization on Ferromagnetic Support Material for Oil Palm\nEmpty Fruit Bunches (OPEFB) Pretreatment","volume":"627","author":"Rashid","year":"2019","journal-title":"IOP\nConference Series: Materials Science and Engineering"},{"issue":"8","key":"2026090307181006300_cit43","doi-asserted-by":"publisher","first-page":"1766","DOI":"10.3390\/polym12081766","article-title":"Halloysite Nanotubes\nCoated by Chitosan for the Controlled Release of Khellin","volume":"12","author":"Lisuzzo","year":"2020","journal-title":"Polymers"},{"key":"2026090307181006300_cit44","doi-asserted-by":"publisher","first-page":"106719","DOI":"10.1016\/j.clay.2022.106719","article-title":"Photostability\nassessment of natural pyrethrins using halloysite nanotube carrier\nsystem","volume":"230","author":"Massaro","year":"2022","journal-title":"Appl. Clay Sci."},{"key":"2026090307181006300_cit45","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1016\/j.procbio.2020.05.003","article-title":"Core-shell Au@Co-Fe hybrid nanoparticles\nas peroxidase mimetic nanozyme for antibacterial application","volume":"95","author":"Mirhosseini","year":"2020","journal-title":"Process Biochemistry"},{"issue":"7","key":"2026090307181006300_cit46","doi-asserted-by":"publisher","first-page":"543","DOI":"10.1038\/nmat2442","article-title":"Understanding biophysicochemical interactions at the\nnano\u2013bio interface","volume":"8","author":"Nel","year":"2009","journal-title":"Nature materials"},{"key":"2026090307181006300_cit47","doi-asserted-by":"publisher","DOI":"10.1016\/j.cej.2025.161230","article-title":"Locally hydrophobic\nand oxytropic nano-bio interface supports highly\neffective enzymatic catalysis: Mechanism and application for enzyme\nactivity evaluation","volume":"509","author":"Ge","year":"2025","journal-title":"Chemical Engineering Journal"},{"issue":"4","key":"2026090307181006300_cit48","doi-asserted-by":"publisher","DOI":"10.1002\/smsc.202300227","article-title":"Nano-bio-interface: unleashing the\npotential of noble\nnanometals","volume":"4","author":"Guo","year":"2024","journal-title":"Small Science"},{"issue":"1","key":"2026090307181006300_cit49","doi-asserted-by":"publisher","first-page":"4950","DOI":"10.1038\/srep04950","article-title":"Amplified\nand selective assay of collagens by enzymatic and fluorescent reactions","volume":"4","author":"Yasmin","year":"2014","journal-title":"Sci. Rep."},{"issue":"41","key":"2026090307181006300_cit50","doi-asserted-by":"publisher","first-page":"17212","DOI":"10.1021\/ja307308j","article-title":"Gold nanoparticles\nas electronic bridges for laccase-based biocathodes","volume":"134","author":"Gutie\u0301rrez-Sa\u0301nchez","year":"2012","journal-title":"J. Am. Chem. Soc."},{"issue":"13","key":"2026090307181006300_cit51","doi-asserted-by":"publisher","first-page":"3813","DOI":"10.3390\/molecules26133813","article-title":"A Brief\nHistory of Colour, the Environmental Impact\nof Synthetic Dyes and Removal by Using Laccases","volume":"26","author":"Ardila-Leal","year":"2021","journal-title":"Molecules"},{"key":"2026090307181006300_cit52","doi-asserted-by":"publisher","first-page":"107271","DOI":"10.1016\/j.clay.2024.107271","article-title":"Cyclodextrin-grafted-hectorite based nanomaterial for antibiotics\nand metal ions adsorption","volume":"250","author":"Massaro","year":"2024","journal-title":"Appl. Clay Sci."},{"key":"2026090307181006300_cit53","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2022.137478","article-title":"Tailor-made\nnovel electrospun polycaprolactone\/polyethyleneimine fiber membranes\nfor laccase immobilization: An all-in-one material to biodegrade textile\ndyes and phenolic compounds","volume":"313","author":"Kolak","year":"2023","journal-title":"Chemosphere"},{"key":"2026090307181006300_cit54","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2023.123410","article-title":"Encapsulated\nlaccase in bimetallic Cu\/Zn ZIFs as stable and reusable biocatalyst\nfor decolorization of dye wastewater","volume":"233","author":"Yang","year":"2023","journal-title":"Int. J.\nBiol. Macromol."},{"key":"2026090307181006300_cit55","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcab.2020.101859","article-title":"An efficient decolorization\nof methyl\norange dye by laccase from Marasmiellus palmivorus immobilized on\nchitosan-coated magnetic particles","volume":"30","author":"Silveira","year":"2020","journal-title":"Biocatalysis\nand Agricultural Biotechnology"},{"key":"2026090307181006300_cit56","doi-asserted-by":"publisher","first-page":"208","DOI":"10.1016\/j.carbpol.2018.04.046","article-title":"Chitosan-functionalized\nsupermagnetic halloysite nanotubes for covalent laccase immobilization","volume":"194","author":"Kadam","year":"2018","journal-title":"Carbohydr. Polym."},{"key":"2026090307181006300_cit57","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2020.123088","article-title":"Improved performance of immobilized laccase on Fe3O4@C-Cu2+ nanoparticles\nand its application for biodegradation of dyes","volume":"399","author":"Li","year":"2020","journal-title":"Journal of Hazardous Materials"},{"key":"2026090307181006300_cit58","doi-asserted-by":"publisher","first-page":"78","DOI":"10.1016\/j.ijbiomac.2020.06.009","article-title":"Immobilization of laccase on Sepharose-linked antibody\nsupport for decolourization of phenol red","volume":"161","author":"Zofair","year":"2020","journal-title":"Int.\nJ. Biol. Macromol."},{"issue":"8","key":"2026090307181006300_cit59","doi-asserted-by":"publisher","DOI":"10.1088\/2053-1591\/ab26a5","article-title":"Effective\nremoval of\nmethylene blue and orange II by subsequent immobilized laccase decolorization\non crosslinked polymethacrylate\/carbon nanotubes","volume":"6","author":"Lai","year":"2019","journal-title":"Materials Research Express"},{"key":"2026090307181006300_cit60","doi-asserted-by":"publisher","DOI":"10.1016\/j.apmt.2021.101045","article-title":"Dye removal by laccase-functionalized\nmicromotors","volume":"23","author":"Uygun","year":"2021","journal-title":"Applied Materials Today"},{"issue":"4","key":"2026090307181006300_cit61","doi-asserted-by":"publisher","first-page":"2777","DOI":"10.1021\/acsomega.0c05081","article-title":"Improvement of Laccase Activity Via Covalent Immobilization\nover Mesoporous Silica Coated Magnetic Multiwalled Carbon Nanotubes\nfor the Discoloration of Synthetic Dyes","volume":"6","author":"Habimana","year":"2021","journal-title":"ACS\nOmega"}],"container-title":["ACS Omega"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsomega.6c06020","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/acsodf\/article-pdf\/11\/34\/51690\/66648335\/acsomega.6c06020.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/acsodf\/article-pdf\/11\/34\/51690\/66648335\/acsomega.6c06020.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T11:18:19Z","timestamp":1788434299000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/acsodf\/article\/11\/34\/51690\/5262041\/Halloysite-Nanotubes-as-Dynamic-Interfaces-for"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,17]]},"references-count":61,"journal-issue":{"issue":"34","published-online":{"date-parts":[[2026,8,17]]},"published-print":{"date-parts":[[2026,9,1]]}},"URL":"https:\/\/doi.org\/10.1021\/acsomega.6c06020","relation":{},"ISSN":["2470-1343"],"issn-type":[{"value":"2470-1343","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,17]]}}}