{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T02:19:45Z","timestamp":1793499585914,"version":"4.5.0"},"reference-count":61,"publisher":"American Chemical Society (ACS)","issue":"32","license":[{"start":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T00:00:00Z","timestamp":1786060800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004901","name":"Funda??o de Amparo ? Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Institute of Science and Technology in Plant-Pest Interaction, Universidade Federal de Vi?osa"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The fall armyworm, Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae), is a significant agricultural pest, and its management is increasingly impeded by resistance to conventional insecticides, underscoring the need for alternative control strategies. This research examined the effects of the synthetic peptide GORE3 on digestive protease homeostasis and larval performance using an integrated approach that combined molecular dynamics simulations, enzymatic assays, physiological analyses, and histopathology. Comparative analyses employed benzamidine and soybean Kunitz trypsin inhibitor (SKTI) as reference inhibitors. Molecular dynamics simulations demonstrated that the trypsin-GORE3 complex is highly stable, with lower Root Mean Square Deviation (RMSD) values and sustained intermolecular interactions compared with benzamidine, while maintaining a favorable binding free-energy profile. In vivo analyses demonstrated that GORE3 induces a dynamic regulation of midgut digestive enzymes, initially suppressing activity, followed by a significant compensatory enhancement in trypsin-like and overall protease activities. Despite this enzymatic compensation, caterpillars exposed to GORE3 has low survival rates, reduced larval weight, prolonged developmental periods, and impaired food conversion efficiency. Nutritional indices indicated a breakdown between digestion and biomass conversion, implying metabolic costs linked to compensatory responses. Histopathological investigations identified midgut damage, including epithelial disruption, epithelial cell disorganization, and structural deterioration with all trypsin inhibitors tested. Oxidative stress responses were detected but played a secondary role relative to digestive dysregulation. Overall, GORE3 disrupts digestive homeostasis by triggering a compensatory yet ineffective proteolytic response, thereby reducing caterpillar fitness. These findings underscore the potential of synthetic peptides targeting digestive proteases as potential bioinsecticides and establish a mechanistic framework linking molecular interactions to organismal effects.<\/jats:p>","DOI":"10.1021\/acsomega.6c06048","type":"journal-article","created":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T14:01:49Z","timestamp":1786111309000},"page":"48583-48601","source":"Crossref","is-referenced-by-count":3,"title":["An Autolysis-Derived\nTrypsin Peptide Disrupts Digestive\nHomeostasis and Reduces Fitness in\n                    <i>Spodoptera Frugiperda<\/i>\n                    Caterpillars"],"prefix":"10.1021","volume":"11","author":[{"given":"Daniel Guimara\u0303es Silva","family":"Paulo","sequence":"first","affiliation":[{"name":"Universidade Federal de Vic\u0327osa, UFV, Bioagro\/INCT-IPP , , , ,","place":["Vic\u0327osa, Minas Gerais, Brazil, 36570-000"]},{"name":"Federal University of Vic\u0327osa , , , ,","place":["Vic\u0327osa, Minas Gerais, Brazil, 36570-000"]},{"name":"Institute of Biotechnology Applied to Agriculture \u2212 BIOAGRO-UFV , ,","place":["Vic\u0327osa, Brazil, 36570-000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5712-9759","authenticated-orcid":true,"given":"Yaremis","family":"Merin\u0303o-Cabrera","sequence":"additional","affiliation":[{"name":"Universidade Federal de Vic\u0327osa, UFV, Bioagro\/INCT-IPP , , , ,","place":["Vic\u0327osa, Minas Gerais, Brazil, 36570-000"]},{"name":"Institute of Biotechnology Applied to Agriculture \u2212 BIOAGRO-UFV , ,","place":["Vic\u0327osa, Brazil, 36570-000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose\u0301","family":"Severiche-Castro","sequence":"additional","affiliation":[{"name":"Universidad del Valle , , ,","place":["Cali, Colombia, 760032"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo Fernandes","family":"Braga","sequence":"additional","affiliation":[{"name":"Federal University of Vic\u0327osa , , , ,","place":["Vic\u0327osa, Minas Gerais, Brazil, 36570-000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanna dos Santos","family":"Pereira","sequence":"additional","affiliation":[{"name":"Universidade Federal de Vic\u0327osa , , ,","place":["Vic\u0327osa, Brazil, 36570-000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laryssa Lemos","family":"da Silva","sequence":"additional","affiliation":[{"name":"Universidade Federal de Vic\u0327osa , , ,","place":["Vic\u0327osa, Brazil, 36570-000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wesley Borges","family":"Wurlitzer","sequence":"additional","affiliation":[{"name":"Universidade do Vale do Taquari, Univates , , , ,","place":["Lajeado, Rio Grande do Sul, Brazil, 95900-000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia Renata","family":"Schneider","sequence":"additional","affiliation":[{"name":"Universidade Federal de Vic\u0327osa, UFV, Bioagro\/INCT-IPP , , , ,","place":["Vic\u0327osa, Minas Gerais, Brazil, 36570-000"]},{"name":"Universidade do Vale do Taquari, Univates , , , ,","place":["Lajeado, Rio Grande do Sul, Brazil, 95900-000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eula\u0301lio Gutemberg Dias","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Universidade Federal de Vic\u0327osa, UFV, Bioagro\/INCT-IPP , , , ,","place":["Vic\u0327osa, Minas Gerais, Brazil, 36570-000"]},{"name":"Institute of Biotechnology Applied to Agriculture \u2212 BIOAGRO-UFV , ,","place":["Vic\u0327osa, Brazil, 36570-000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noeli Juarez","family":"Ferla","sequence":"additional","affiliation":[{"name":"Universidade do Vale do Taquari, Univates , , , ,","place":["Lajeado, Rio Grande do Sul, Brazil, 95900-000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0477-4252","authenticated-orcid":true,"given":"Jose\u0301 Eduardo","family":"Serra\u0303o","sequence":"additional","affiliation":[{"name":"Universidade Federal de Vic\u0327osa , , ,","place":["Vic\u0327osa, Brazil, 36570-000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael Ju\u0301nior","family":"de Andrade","sequence":"additional","affiliation":[{"name":"Universidade Federal de Vic\u0327osa, UFV, Bioagro\/INCT-IPP , , , ,","place":["Vic\u0327osa, Minas Gerais, Brazil, 36570-000"]},{"name":"Institute of Biotechnology Applied to Agriculture \u2212 BIOAGRO-UFV , ,","place":["Vic\u0327osa, Brazil, 36570-000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ota\u0301vio Jose\u0301 Bernardes","family":"Brustolini","sequence":"additional","affiliation":[{"name":"National Laboratory for Scientific Computing , , , ,","place":["Petro\u0301polis, Rio de Janeiro, Brazil, 25651-075"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neilier Rodrigues da Silva","family":"Ju\u0301nior","sequence":"additional","affiliation":[{"name":"Universidade Federal de Vic\u0327osa, UFV, Bioagro\/INCT-IPP , , , ,","place":["Vic\u0327osa, Minas Gerais, Brazil, 36570-000"]},{"name":"Institute of Biotechnology Applied to Agriculture \u2212 BIOAGRO-UFV , ,","place":["Vic\u0327osa, Brazil, 36570-000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Humberto Josue\u0301 Oliveira","family":"Ramos","sequence":"additional","affiliation":[{"name":"Universidade Federal de Vic\u0327osa, UFV, Bioagro\/INCT-IPP , , , ,","place":["Vic\u0327osa, Minas Gerais, Brazil, 36570-000"]},{"name":"Institute of Biotechnology Applied to Agriculture \u2212 BIOAGRO-UFV , ,","place":["Vic\u0327osa, Brazil, 36570-000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4862-7657","authenticated-orcid":true,"given":"Maria Goreti de Almeida","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Universidade Federal de Vic\u0327osa, UFV, Bioagro\/INCT-IPP , , , ,","place":["Vic\u0327osa, Minas Gerais, Brazil, 36570-000"]},{"name":"Institute of Biotechnology Applied to Agriculture \u2212 BIOAGRO-UFV , ,","place":["Vic\u0327osa, Brazil, 36570-000"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,7]]},"reference":[{"issue":"6","key":"2026081811124638900_cit1","doi-asserted-by":"publisher","first-page":"1772","DOI":"10.1093\/jee\/toac089","article-title":"Potential Management\nOptions for the Invasive Moth Spodoptera frugiperda in Europe","volume":"115","author":"Babendreier","year":"2022","journal-title":"J. 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Entomol."},{"issue":"6","key":"2026081811124638900_cit2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0324857","article-title":"First report\nof field-evolved resistance to insecticides\nin Spodoptera frugiperda (Lepidoptera: Noctuidae) from Punjab, Pakistan","volume":"20","author":"Khan","year":"2025","journal-title":"PLoS One"},{"issue":"9","key":"2026081811124638900_cit3","doi-asserted-by":"publisher","first-page":"705","DOI":"10.3390\/insects15090705","article-title":"Monitoring and\nDetection of Insecticide Resistance in Spodoptera frugiperda (Lepidoptera:\nNoctuidae): Evidence for Field-Evolved Resistance in Egypt","volume":"15","author":"Moustafa","year":"2024","journal-title":"Insects"},{"issue":"1","key":"2026081811124638900_cit4","doi-asserted-by":"publisher","first-page":"409","DOI":"10.1007\/s42690-025-01691-5","article-title":"Synthetic or biological insecticide performance on\nBt corn to control Spodoptera frugiperda","volume":"46","author":"Giacomelli","year":"2026","journal-title":"Int.\nJ. Trop Insect Sci."},{"issue":"1","key":"2026081811124638900_cit5","doi-asserted-by":"publisher","DOI":"10.1002\/arch.21631","article-title":"Intestinal proteolytic profile changes during larval\ndevelopment of Anticarsia gemmatalis caterpillars","volume":"103","author":"da Silva Ju\u0301nior","year":"2020","journal-title":"Arch. Insect Biochem. Physiol."},{"key":"2026081811124638900_cit6","doi-asserted-by":"publisher","first-page":"105188","DOI":"10.1016\/j.pestbp.2022.105188","article-title":"Bovine pancreatic trypsin\ninhibitor and soybean Kunitz trypsin inhibitor: Differential effects\non proteases and larval development of the soybean pest Anticarsia\ngemmatalis (Lepidoptera: Noctuidae)","volume":"187","author":"de Almeida Barros","year":"2022","journal-title":"Pestic.\nBiochem. Physiol."},{"issue":"5","key":"2026081811124638900_cit7","doi-asserted-by":"publisher","first-page":"823","DOI":"10.1007\/s12600-020-00837-0","article-title":"Inhibitory effects of tripeptides to enzymatic activity\nand life cycle parameters of Anticarsia gemmatalis","volume":"48","author":"de Oliveira","year":"2020","journal-title":"Phytoparasitica"},{"issue":"3","key":"2026081811124638900_cit8","doi-asserted-by":"publisher","first-page":"383","DOI":"10.1111\/aab.12740","article-title":"Extensive\nreprogramming\nof protein isoforms and histopathological alterations in the midgut\nof Anticarsia gemmatalis fed with protease inhibitors","volume":"180","author":"Coura","year":"2022","journal-title":"Ann. Appl. Biol."},{"issue":"1","key":"2026081811124638900_cit9","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1007\/s12600-024-01125-x","article-title":"Inhibitory efficacy\nof tripeptides on trypsin-like activity in soybean caterpillars Anticarsia\ngemmatalis (Lepidoptera: Erebidae) with dysbiosis","volume":"52","author":"Schultz","year":"2024","journal-title":"Phytoparasitica"},{"issue":"3","key":"2026081811124638900_cit10","doi-asserted-by":"publisher","DOI":"10.1002\/arch.70145","article-title":"Synthetic Peptide Inhibition\nof Trypsin-Like Proteases in Spodoptera frugiperda (Lepidoptera: Noctuidae):\nEvaluating the Influence of Gut Microbiota","volume":"121","author":"Schultz","year":"2026","journal-title":"Arch. Insect Biochem. Physiol."},{"key":"2026081811124638900_cit11","doi-asserted-by":"publisher","first-page":"151886","DOI":"10.1016\/j.ijbiomac.2026.151886","article-title":"Expression\nof a recombinant peptide with bioinsecticidal potential for the control\nof agricultural pests","volume":"359","author":"de Andrade","year":"2026","journal-title":"Int. J. Biol. Macromol."},{"key":"2026081811124638900_cit12","doi-asserted-by":"publisher","first-page":"4632","DOI":"10.1002\/ps.70579","article-title":"Peptidic\nproduct derived from trypsin autolysis modulates insect digestive\nproteases and supports plant biochemical defense","volume":"82","author":"Paulo","year":"2026","journal-title":"Pest Manage. Sci."},{"key":"2026081811124638900_cit13","doi-asserted-by":"publisher","first-page":"103390","DOI":"10.1016\/j.ibmb.2020.103390","article-title":"Rational design of mimetic\npeptides based on the interaction between Inga laurina inhibitor and\ntrypsins for Spodoptera cosmioides pest control","volume":"122","author":"Merin\u0303o-Cabrera","year":"2020","journal-title":"Insect Biochem. Mol. Biol."},{"issue":"3","key":"2026081811124638900_cit14","doi-asserted-by":"publisher","first-page":"1677","DOI":"10.1007\/s10989-021-10200-4","article-title":"Inhibitory Potential\nof a Designed Peptide Inhibitor Based on Zymogen Structure of Trypsin\nfrom Spodoptera frugiperda: In Silico Insights","volume":"27","author":"Hemmati","year":"2021","journal-title":"Int. J. Pept. Res. Ther."},{"issue":"3","key":"2026081811124638900_cit15","doi-asserted-by":"publisher","first-page":"336","DOI":"10.1111\/aab.12539","article-title":"Inhibition of digestive trypsins by plant Kunitz proteins\nreduces the viability of Spodoptera cosmioides larvae","volume":"175","author":"Merin\u0303o-Cabrera","year":"2019","journal-title":"Ann. Appl. Biol."},{"issue":"3","key":"2026081811124638900_cit16","doi-asserted-by":"publisher","DOI":"10.1002\/arch.21687","article-title":"Noncompetitive tight-binding\ninhibition of Anticarsia gemmatalis trypsins by Adenanthera pavonina\nprotease inhibitor affects larvae survival","volume":"104","author":"Merin\u0303o-Cabrera","year":"2020","journal-title":"Arch. Insect Biochem. Physiol."},{"issue":"1","key":"2026081811124638900_cit17","doi-asserted-by":"publisher","DOI":"10.1002\/arch.70123","article-title":"Peptides Derived From Reactive Center Loops Inhibit\nDigestive Trypsin-Like Enzymes in Lepidopteran Pests","volume":"121","author":"Paulo","year":"2026","journal-title":"Arch. Insect Biochem. Physiol."},{"issue":"4","key":"2026081811124638900_cit18","doi-asserted-by":"publisher","first-page":"1714","DOI":"10.1002\/ps.6191","article-title":"Small peptides inhibit gut trypsin-like proteases and\nimpair Anticarsia gemmatalis (Lepidoptera: Noctuidae) survival and\ndevelopment","volume":"77","author":"de Almeida Barros","year":"2021","journal-title":"Pest Manage. Sci."},{"key":"2026081811124638900_cit19","doi-asserted-by":"publisher","first-page":"1963","DOI":"10.3389\/fphys.2018.01963","article-title":"Inhibitory Effect of\nProtease Inhibitors on Larval Midgut Protease Activities and the Performance\nof Plutella xylostella (Lepidoptera: Plutellidae)","volume":"9","author":"Zhao","year":"2018","journal-title":"Front. Physiol."},{"issue":"2","key":"2026081811124638900_cit20","doi-asserted-by":"publisher","first-page":"312","DOI":"10.1021\/acsmedchemlett.1c00717","article-title":"Imidazoline- and Benzamidine-Based\nTrypanosome Alternative Oxidase Inhibitors: Synthesis and Structure\u2013Activity\nRelationship Studies","volume":"13","author":"Cisneros","year":"2022","journal-title":"ACS Med. Chem. Lett."},{"key":"2026081811124638900_cit21","doi-asserted-by":"publisher","first-page":"119","DOI":"10.14411\/eje.2025.015","article-title":"Differential gene expression reprogramming in the midgut\nof Anticarsia gemmatalis (Lepidoptera: Noctuidae) triggered by an\nSKTI-derivative tripeptide protease inhibitor compared to the natural\nSKTI protein","volume":"122","author":"dos Santos","year":"2025","journal-title":"Eur. J. Entomol."},{"issue":"11","key":"2026081811124638900_cit22","doi-asserted-by":"publisher","first-page":"787","DOI":"10.3390\/catal14110787","article-title":"Advancements in Serine Protease Inhibitors:\nFrom Mechanistic Insights to Clinical Applications","volume":"14","author":"Wei","year":"2024","journal-title":"Catalysts"},{"issue":"10","key":"2026081811124638900_cit23","doi-asserted-by":"publisher","first-page":"4303","DOI":"10.1021\/acschemneuro.9b00348","article-title":"Molecular Mechanism of Binding Selectivity of Inhibitors toward BACE1\nand BACE2 Revealed by Multiple Short Molecular Dynamics Simulations\nand Free-Energy Predictions","volume":"10","author":"Chen","year":"2019","journal-title":"ACS Chem. Neurosci."},{"issue":"40","key":"2026081811124638900_cit24","doi-asserted-by":"publisher","first-page":"10256","DOI":"10.1021\/acs.jpcb.5c04050","article-title":"Conformational Transition and Recognition Mechanism of Eukaryotic\nRiboswitches Powered by Thiamine Pyrophosphate Analogues: An Elucidation\nthrough Multiple Short Molecular Dynamics Simulations and Markov Model","volume":"129","author":"Chen","year":"2025","journal-title":"J. Phys. Chem. B"},{"key":"2026081811124638900_cit25","doi-asserted-by":"publisher","DOI":"10.1590\/0001-3765202020180477","article-title":"Inhibition\nkinetics of digestive proteases for Anticarsia\ngemmatalis","volume":"92","author":"Patarroyo-Vargas","year":"2020","journal-title":"An. Acad. Bras. Cienc."},{"issue":"11","key":"2026081811124638900_cit26","doi-asserted-by":"publisher","first-page":"1040","DOI":"10.2174\/0929866524666170918103146","article-title":"Kinetic Characterization of Anticarsia\ngemmatalis Digestive Serine- Proteases and the Inhibitory Effect of\nSynthetic Peptides","volume":"24","author":"Patarroyo-Vargas","year":"2017","journal-title":"Protein Pept. Lett."},{"issue":"3","key":"2026081811124638900_cit27","doi-asserted-by":"publisher","first-page":"369","DOI":"10.1016\/j.cbpc.2004.10.018","article-title":"Partial purification\nand characterization of digestive trypsin-like proteases from the\nvelvet bean caterpillar, Anticarsia gemmatalis","volume":"140","author":"Oliveira","year":"2005","journal-title":"Comp. Biochem. Physiol. B"},{"issue":"3","key":"2026081811124638900_cit28","doi-asserted-by":"publisher","first-page":"611","DOI":"10.1111\/1744-7917.12906","article-title":"Role of\ndigestive protease enzymes and related genes in host plant\nadaptation of a polyphagous pest, Spodoptera frugiperda","volume":"28","author":"Hafeez","year":"2021","journal-title":"Insect Sci."},{"issue":"4","key":"2026081811124638900_cit29","doi-asserted-by":"publisher","first-page":"487","DOI":"10.1093\/jee\/69.4.487","article-title":"Velvetbean\nCaterpillar: A Rearing\nProcedure and Artificial Medium123","volume":"69","author":"Greene","year":"1976","journal-title":"J. Econ.\nEntomol."},{"issue":"2","key":"2026081811124638900_cit30","doi-asserted-by":"publisher","DOI":"10.1002\/arch.21887","article-title":"Inhibition constant and stability\nof tripeptide inhibitors of gut trypsin-like enzyme of the soybean\npest Anticarsia gemmatalis","volume":"110","author":"de Almeida Barros","year":"2022","journal-title":"Arch. Insect Biochem.\nPhysiol."},{"issue":"1\u20132","key":"2026081811124638900_cit31","doi-asserted-by":"publisher","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid\nand sensitive method for the quantitation of microgram quantities\nof protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"issue":"1","key":"2026081811124638900_cit32","doi-asserted-by":"publisher","first-page":"70","DOI":"10.1006\/abio.1996.0292","article-title":"The Ferric Reducing\nAbility of Plasma (FRAP) as a Measure\nof \u201cAntioxidant Power\u201d: The FRAP Assay","volume":"239","author":"Benzie","year":"1996","journal-title":"Anal. Biochem."},{"key":"2026081811124638900_cit33","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1016\/S0076-6879(84)05016-3","article-title":"Catalase in\nvitro","volume":"105","author":"Aebi","year":"1984","journal-title":"In"},{"issue":"2\u20133","key":"2026081811124638900_cit34","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1016\/0009-8981(91)90067-M","article-title":"A simple method\nfor determination of serum catalase activity and revision of reference\nrange","volume":"196","author":"Go\u0301th","year":"1991","journal-title":"Clin. Chim. Acta"},{"issue":"3","key":"2026081811124638900_cit35","doi-asserted-by":"publisher","first-page":"469","DOI":"10.1111\/j.1432-1033.1974.tb03714.x","article-title":"Involvement of the\nSuperoxide Anion Radical in the\nAutoxidation of Pyrogallol and a Convenient Assay for Superoxide Dismutase","volume":"47","author":"Marklund","year":"1974","journal-title":"Eur. J. Biochem."},{"key":"2026081811124638900_cit36","doi-asserted-by":"publisher","first-page":"346","DOI":"10.1016\/S0076-6879(94)33040-9","article-title":"Carbonyl assays for\ndetermination of oxidatively modified proteins","volume":"233","author":"Levine","year":"1994","journal-title":"Methods Enzymol."},{"key":"2026081811124638900_cit37","doi-asserted-by":"publisher","first-page":"302","DOI":"10.1016\/S0076-6879(78)52032-6","article-title":"[30] Microsomal lipid peroxidation","volume":"52","author":"Buege","year":"1978","journal-title":"Methods Enzymol."},{"issue":"2","key":"2026081811124638900_cit38","doi-asserted-by":"publisher","first-page":"170","DOI":"10.1002\/ps.2047","article-title":"Lepidopteran larva consumption of\nsoybean foliage: basis for developing multiple-species economic thresholds\nfor pest management decisions","volume":"67","author":"de Freitas Bueno","year":"2011","journal-title":"Pest Manage.\nSci."},{"issue":"11","key":"2026081811124638900_cit39","doi-asserted-by":"publisher","first-page":"1125","DOI":"10.2174\/1389557523666230901152257","article-title":"The Power\nof Molecular Dynamics Simulations and Their Applications to Discover\nCysteine Protease Inhibitors","volume":"24","author":"dos Santos Nascimento","year":"2024","journal-title":"Mini-Rev. 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