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Here, we used a simple rotational exposure system as an in vitro proof-of-concept to test 80 nm polystyrene (PS) nanoplastics in A549 cells. Exposure to 100 \u03bcg\/L PS nanoplastics or rotation at 6.7 rpm alone produced no detectable change in tetramethylrhodamine ethyl ester (TMRE) fluorescence or intracellular reactive oxygen species (ROS) under the conditions tested. In contrast, the combined treatment increased TMRE fluorescence and induced a pronounced ROS response without altering the mitochondrial-to-nuclear DNA ratio. Separately, an idealized particle-impact model predicted that single-particle impact force increases strongly with particle size and flow velocity at arterial bifurcations. Because the rotation speed was not calibrated to physiological wall shear stress, and A549 cells are not endothelial cells, these findings do not establish vascular toxicity. Rather, they show that particle motion can modify cellular responses to PS nanoplastics in vitro and support further testing in calibrated endothelial flow systems.<\/jats:p>","DOI":"10.1021\/acsomega.6c06111","type":"journal-article","created":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T13:01:36Z","timestamp":1789390896000},"page":"57541-57547","source":"Crossref","is-referenced-by-count":0,"title":["Rotational Motion\nAmplifies Oxidative Responses to\nPolystyrene Nanoplastics: An\n                    <i>In Vitro<\/i>\n                    Proof-of-Concept\nand Particle-Impact Model"],"prefix":"10.1021","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-2075-1613","authenticated-orcid":true,"given":"Hanzeng","family":"Li","sequence":"first","affiliation":[{"name":"Hainan University , , , ,","place":["Haikou, Hainan Province, China, 570228"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Zhou","sequence":"additional","affiliation":[{"name":"Hainan University , , , ,","place":["Haikou, Hainan Province, China, 570228"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofeng","family":"Chen","sequence":"additional","affiliation":[{"name":"Hainan University , , , ,","place":["Haikou, Hainan Province, China, 570228"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shunqing","family":"Xu","sequence":"additional","affiliation":[{"name":"Hainan University , , , ,","place":["Haikou, Hainan Province, China, 570228"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,14]]},"reference":[{"key":"2026092911093364700_cit1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2020.101545","article-title":"Oxidative\nstress pathways of air pollution mediated toxicity: recent insights","volume":"34","author":"Gangwar","year":"2020","journal-title":"Redox Biol."},{"issue":"4","key":"2026092911093364700_cit2","doi-asserted-by":"publisher","first-page":"308","DOI":"10.1089\/ars.2019.8015","article-title":"Particulate\nmatter induces tissue oxInflammation: from mechanism to damage","volume":"33","author":"Valacchi","year":"2020","journal-title":"Antioxid. 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Saf."},{"key":"2026092911093364700_cit7","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2025.103688","article-title":"Mitigating\nmicroplastic-induced\norgan damage: mechanistic insights from the microplastic-macrophage\naxes","volume":"84","author":"Cui","year":"2025","journal-title":"Redox Biol."},{"key":"2026092911093364700_cit8","doi-asserted-by":"publisher","first-page":"211","DOI":"10.1016\/j.envpol.2014.12.021","article-title":"Pollutants bioavailability\nand toxicological risk from\nmicroplastics to marine mussels","volume":"198","author":"Avio","year":"2015","journal-title":"Environ. 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Care Med."},{"key":"2026092911093364700_cit30","doi-asserted-by":"publisher","first-page":"823","DOI":"10.1080\/15376516.2025.2500546","article-title":"Effects of micro- and\nnanoplastic exposure on macrophages: a review of molecular and cellular\nmechanisms","volume":"35","author":"Ahmadi","year":"2025","journal-title":"Toxicol. Mech. Methods"},{"issue":"1","key":"2026092911093364700_cit31","doi-asserted-by":"publisher","DOI":"10.1186\/s43591-025-00138-5","article-title":"Top-down generated\nmicro- and nanoplastics reduce macrophage viability without eliciting\na pro-inflammatory response","volume":"5","author":"van den Berg","year":"2025","journal-title":"Microplast. Nanoplast."},{"issue":"7","key":"2026092911093364700_cit32","doi-asserted-by":"publisher","first-page":"5489","DOI":"10.1021\/acsnano.5c15425","article-title":"Microplastic-induced\nmacrophage dysfunction drives lung tumor progression through glutathione\nimbalance","volume":"20","author":"Kim","year":"2026","journal-title":"ACS Nano"},{"key":"2026092911093364700_cit33","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2024.133855","article-title":"Microplastics in three\ntypes of human arteries detected\nby pyrolysis-gas chromatography\/mass spectrometry (Py-GC\/MS)","volume":"469","author":"Liu","year":"2024","journal-title":"J. Hazard. Mater."},{"key":"2026092911093364700_cit34","doi-asserted-by":"publisher","DOI":"10.1016\/j.envint.2025.109743","article-title":"Tissue-specific distribution of microplastics in human\nblood and\ncarotid plaques: a paired sample analysis","volume":"203","author":"Cui","year":"2025","journal-title":"Environ.\nInt."},{"issue":"1","key":"2026092911093364700_cit35","doi-asserted-by":"publisher","DOI":"10.1186\/s12872-025-05330-2","article-title":"From pollution to\npalpitations: the heart\u2019s silent battle with microplastics","volume":"25","author":"Jahedi","year":"2025","journal-title":"BMC Cardiovasc. Disord."},{"key":"2026092911093364700_cit36","doi-asserted-by":"publisher","DOI":"10.1016\/j.envint.2022.107662","article-title":"Micro- and nanoplastics:\na new cardiovascular risk factor?","volume":"171","author":"Zhu","year":"2023","journal-title":"Environ.\nInt."},{"issue":"38","key":"2026092911093364700_cit37","doi-asserted-by":"publisher","first-page":"4099","DOI":"10.1093\/eurheartj\/ehae552","article-title":"Micro-nanoplastics and\ncardiovascular diseases: evidence and perspectives","volume":"45","author":"Prattichizzo","year":"2024","journal-title":"Eur. Heart J."},{"issue":"7","key":"2026092911093364700_cit38","doi-asserted-by":"publisher","first-page":"752","DOI":"10.1161\/CIRCRESAHA.124.325685","article-title":"Biophysical and biochemical\nroles of shear stress on endothelium: a revisit and new insights","volume":"136","author":"Cheng","year":"2025","journal-title":"Circ. 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Mol.\nLife Sci."},{"key":"2026092911093364700_cit48","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxrep.2025.102043","article-title":"Micro- and nanoplastic toxicity in\nhumans: exposure\npathways, cellular effects, and mitigation strategies","volume":"14","author":"Jahedi","year":"2025","journal-title":"Toxicol. Rep."},{"key":"2026092911093364700_cit49","doi-asserted-by":"publisher","first-page":"48","DOI":"10.1016\/j.toxlet.2024.04.004","article-title":"Vertebrate\nresponse to microplastics,\nnanoplastics and co-exposed contaminants: assessing accumulation,\ntoxicity, behaviour, physiology, and molecular changes","volume":"396","author":"Kaur","year":"2024","journal-title":"Toxicol. Lett."},{"key":"2026092911093364700_cit50","doi-asserted-by":"publisher","DOI":"10.3389\/ftox.2026.1766103","article-title":"Microplastic\ntoxicity: mechanisms,\nassessment methods, and future research directions","volume":"8","author":"Zhang","year":"2026","journal-title":"Front. 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Pollut.\nRes."}],"container-title":["ACS Omega"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsomega.6c06111","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/acsodf\/article-pdf\/11\/38\/57541\/68498372\/acsomega.6c06111.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/acsodf\/article-pdf\/11\/38\/57541\/68498372\/acsomega.6c06111.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T15:51:09Z","timestamp":1790697069000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/acsodf\/article\/11\/38\/57541\/5429314\/Rotational-Motion-Amplifies-Oxidative-Responses-to"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,14]]},"references-count":51,"journal-issue":{"issue":"38","published-online":{"date-parts":[[2026,9,14]]},"published-print":{"date-parts":[[2026,9,29]]}},"URL":"https:\/\/doi.org\/10.1021\/acsomega.6c06111","relation":{},"ISSN":["2470-1343"],"issn-type":[{"value":"2470-1343","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,14]]}}}