{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T12:13:13Z","timestamp":1788437593082,"version":"build-2803163510"},"reference-count":42,"publisher":"American Chemical Society (ACS)","issue":"34","license":[{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004704","name":"National Research Council of Thailand","doi-asserted-by":"publisher","award":["N84A680764"],"award-info":[{"award-number":["N84A680764"]}],"id":[{"id":"10.13039\/501100004704","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Citrullinated histone H3 (CitH3) is an emerging biomarker for early sepsis diagnosis. However, its detection based on standard enzyme-linked immunosorbent assay (ELISA) suffers from long analysis time (4\u20135 h), expensive instruments, and the need for skilled operators, necessitating the development of alternative detection platforms. Herein, a portable electrochemical immunosensor for CitH3 determination was developed using a screen-printed graphene electrode coupled with a custom-designed potentiostat. CitH3 was detected via a sandwich immunoassay employing horseradish peroxidase\u2013conjugated antibody and amperometric measurement in the presence of hydroquinone and hydrogen peroxide. Key fabrication and detection parameters were systematically optimized to achieve high analytical performance. Under optimal conditions, the immunosensor exhibited a linear detection range of 0.1\u20131000 ng mL\u20131 with a detection limit of 42.83 pg mL\u20131 and a quantification limit of 100.89 pg mL\u20131, enabling the discrimination between sepsis and nonsepsis conditions. Additionally, the developed method provided a lower detection limit and shorter processing time (2 h) compared with ELISA. It also demonstrated high selectivity against common serum interferents, satisfactory accuracy (recovery = 89.49\u2013106.11%), and good repeatability (relative standard deviation &amp;lt; 10%). Analysis of clinical serum samples (n = 50) showed a high correlation coefficient with ELISA (r = 0.9861), and Bland\u2013Altman analysis confirmed their good agreement. Furthermore, the assay diagnostic performance demonstrated 100% specificity with high sensitivity values of 90.01% and 81.25% for sepsis and severe sepsis cases, respectively. These results suggested the potential of the proposed portable electrochemical immunosensor as a practical tool for sepsis diagnosis.<\/jats:p>","DOI":"10.1021\/acsomega.6c06505","type":"journal-article","created":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T08:02:23Z","timestamp":1787126543000},"page":"51893-51904","source":"Crossref","is-referenced-by-count":0,"title":["Electrochemical\nImmunosensor for Point-of-Care Detection\nof Citrullinated Histone H3 Using Screen-Printed Graphene Electrodes\nand a Portable Potentiostat"],"prefix":"10.1021","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9342-8011","authenticated-orcid":true,"given":"Vitsarut","family":"Primpray","sequence":"first","affiliation":[{"name":"National Science and Technology Development Agency (NSTDA) , , ,","place":["Pathum Thani, Thailand, 12120"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wichayaporn","family":"Kamsong","sequence":"additional","affiliation":[{"name":"National Science and Technology Development Agency (NSTDA) , , ,","place":["Pathum Thani, Thailand, 12120"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Assawapong","family":"Sappat","sequence":"additional","affiliation":[{"name":"National Science and Technology Development Agency (NSTDA) , , ,","place":["Pathum Thani, Thailand, 12120"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theeraphud","family":"Phunin","sequence":"additional","affiliation":[{"name":"National Science and Technology Development Agency (NSTDA) , , ,","place":["Pathum Thani, Thailand, 12120"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anurat","family":"Wisitsoraat","sequence":"additional","affiliation":[{"name":"National Science and Technology Development Agency (NSTDA) , , ,","place":["Pathum Thani, Thailand, 12120"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Awirut","family":"Charoensappakit","sequence":"additional","affiliation":[{"name":"Chulalongkorn University , , ,","place":["Bangkok, Thailand, 10330"]},{"name":"Chulalongkorn University , , ,","place":["Bangkok, Thailand, 10330"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kritsanawan","family":"Sae-khow","sequence":"additional","affiliation":[{"name":"Chulalongkorn University , , ,","place":["Bangkok, Thailand, 10330"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asada","family":"Leelahavanichkul","sequence":"additional","affiliation":[{"name":"Chulalongkorn University , , ,","place":["Bangkok, Thailand, 10330"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chanpen","family":"Karuwan","sequence":"additional","affiliation":[{"name":"National Science and Technology Development Agency (NSTDA) , , ,","place":["Pathum Thani, Thailand, 12120"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,19]]},"reference":[{"key":"2026090307180781700_cit1","doi-asserted-by":"publisher","first-page":"2120","DOI":"10.3390\/ijms17122120","article-title":"Microbial\nEtiology of Pneumonia: Epidemiology, Diagnosis and Resistance Patterns","volume":"17","author":"Cilloniz","year":"2016","journal-title":"Int. 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