{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T16:01:02Z","timestamp":1791302462940,"version":"4.1.0"},"reference-count":20,"publisher":"American Chemical Society (ACS)","issue":"39","license":[{"start":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:00:00Z","timestamp":1790208000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico","doi-asserted-by":"publisher","award":["307299\/2026-0"],"award-info":[{"award-number":["307299\/2026-0"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo","doi-asserted-by":"publisher","award":["2018\/18954-4"],"award-info":[{"award-number":["2018\/18954-4"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo","doi-asserted-by":"publisher","award":["2020\/03399-5"],"award-info":[{"award-number":["2020\/03399-5"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo","doi-asserted-by":"publisher","award":["2023\/18327-8"],"award-info":[{"award-number":["2023\/18327-8"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda??o de Amparo ? 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Current therapies, based on pyrimethamine and sulfadiazine, are limited by toxicity, suboptimal efficacy, and the emergence of resistant parasite strains, highlighting the need for safer and more effective alternatives. In this study, we evaluated the antiparasitic potential of six 2-pyridyl 1,3-thiazole derivatives\u2500privileged scaffolds previously reported for potent antitumor activity but never investigated against T. gondii. In vitro assays were performed using intracellular tachyzoites of the RH strain and human foreskin fibroblasts to determine antiparasitic activity and host-cell cytotoxicity. The derivatives exhibited half-maximal effective concentrations (EC50) between 212 and 490 nM and cytotoxicity values (CC50) ranging from 1.5 to 48 \u03bcM. One compound, TAP-05, demonstrated a notable selectivity index of 183.26, exceeding that of the reference drug pyrimethamine, supporting its potential as a lead candidate for further optimization. Transmission electron microscopy of treated parasites revealed reduced ultrastructural signs of tachyzoite division and increased cytoplasmic vacuolization compared to untreated controls, along with occasional Golgi perturbations. These qualitative observations raise the hypothesis of a cytostatic effect of TAP-05 on parasite replication, which requires confirmation by dedicated functional assays. These findings identify a promising thiazole-based chemotype for toxoplasmosis drug discovery and warrant additional studies to elucidate mechanisms of action and assess in vivo efficacy.<\/jats:p>","DOI":"10.1021\/acsomega.6c06743","type":"journal-article","created":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T05:02:07Z","timestamp":1790226127000},"page":"59807-59814","source":"Crossref","is-referenced-by-count":0,"title":["Highly Selective Thiazole Derivatives as Promising\nAnti-\n                    <i>Toxoplasma gondii<\/i>\n                    Agents"],"prefix":"10.1021","volume":"11","author":[{"given":"Ingrid de Oliveira","family":"Dias","sequence":"first","affiliation":[{"name":"Faculdade de Medicina de Jundiai\u0301 , , Rua Francisco Telles 250 , ,","place":["Jundiai\u0301, Brazil, 13202-550"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1256-057X","authenticated-orcid":true,"given":"Gabriel Ca\u0302ndido","family":"Moura","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina de Jundiai\u0301 , , Rua Francisco Telles 250 , ,","place":["Jundiai\u0301, Brazil, 13202-550"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-4233-4066","authenticated-orcid":true,"given":"Lucas Manoel","family":"da Silva Sousa","sequence":"additional","affiliation":[{"name":"Federal University of Pernambuco , , Avenida Artur de Sa\u0301 S\/N , ,","place":["Recife, Brazil, 50740-520"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael Meyer","family":"Mariante","sequence":"additional","affiliation":[{"name":"Instituto Oswaldo Cruz , , Avenida Brasil 4365 , ,","place":["Rio de Janeiro, Brazil, 21040-360"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9346-2292","authenticated-orcid":true,"given":"Ana Cristina Lima","family":"Leite","sequence":"additional","affiliation":[{"name":"Federal University of Pernambuco , , Avenida Artur de Sa\u0301 S\/N , ,","place":["Recife, Brazil, 50740-520"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4418-0838","authenticated-orcid":true,"given":"Juliana Quero","family":"Reima\u0303o","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina de Jundiai\u0301 , , Rua Francisco Telles 250 , ,","place":["Jundiai\u0301, Brazil, 13202-550"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,24]]},"reference":[{"key":"2026100610480747300_cit1","doi-asserted-by":"publisher","first-page":"3059","DOI":"10.1007\/s00436-018-6040-2","article-title":"Toxoplasma gondii Infection and Behavioral Outcomes\nin Humans: A Systematic Review","volume":"117","author":"Martinez","year":"2018","journal-title":"Parasitol. Res."},{"key":"2026100610480747300_cit2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fawpar.2019.e00049","article-title":"Environmental Transmission\nof Toxoplasma gondii: Oocysts in Water, Soil and\nFood","volume":"15","author":"Shapiro","year":"2019","journal-title":"Food Waterborne Parasitol."},{"key":"2026100610480747300_cit3","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1186\/s13071-020-04528-x","article-title":"Behavioral Biology\nof Toxoplasma gondii Infection","volume":"14","author":"Tong","year":"2021","journal-title":"Parasites Vectors"},{"key":"2026100610480747300_cit4","doi-asserted-by":"publisher","first-page":"110","DOI":"10.3390\/pathogens12010110","article-title":"Advances and\nChallenges in Diagnostics of Toxoplasmosis in HIV-Infected Patients","volume":"12","author":"Weso\u0142owski","year":"2023","journal-title":"Pathogens"},{"key":"2026100610480747300_cit5","doi-asserted-by":"crossref","DOI":"10.7476\/9788575415719","volume-title":"Toxoplasmosis & Toxoplasma gondii","author":"de Souza","year":"2014"},{"key":"2026100610480747300_cit6","doi-asserted-by":"publisher","first-page":"273","DOI":"10.2147\/DDDT.S60973","article-title":"Drugs in Development\nfor Toxoplasmosis: Advances, Challenges,\nand Current Status","volume":"11","author":"Alday","year":"2017","journal-title":"Drug Des. Devel. Ther."},{"key":"2026100610480747300_cit7","doi-asserted-by":"publisher","first-page":"473","DOI":"10.2174\/1386207043328544","article-title":"Privileged\nStructures: Applications in Drug Discovery","volume":"7","author":"Desimone","year":"2004","journal-title":"Comb. Chem. High Throughput Screen."},{"key":"2026100610480747300_cit8","doi-asserted-by":"publisher","first-page":"479","DOI":"10.1080\/17568919.2026.2617612","article-title":"Thiazole scaffolds as\nanticancer and antimicrobial agents: recent advances in medicinal\nchemistry","volume":"18","author":"Nuha","year":"2026","journal-title":"Future Med. Chem."},{"key":"2026100610480747300_cit9","doi-asserted-by":"publisher","first-page":"4323","DOI":"10.2174\/0929867324666171023163752","article-title":"Privileged Structures in the Design of Potential Drug\nCandidates for Neglected Diseases","volume":"26","author":"Leite","year":"2019","journal-title":"Curr. 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Chem."},{"key":"2026100610480747300_cit12","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1016\/j.ejmech.2017.02.026","article-title":"Design and Synthesis of Potent Anti-Trypanosoma cruzi Agents: New Thiazole Derivatives Which\nInduce Apoptotic Parasite Death","volume":"130","author":"da Silva","year":"2017","journal-title":"Eur. J. Med.\nChem."},{"key":"2026100610480747300_cit13","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1016\/j.ijpddr.2021.05.004","article-title":"In vitro evaluation\nof new 4-thiazolidinones on invasion and growth of Toxoplasma gondii","volume":"16","author":"Molina","year":"2021","journal-title":"Int. J. 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Available\nat: https:\/\/www.organic-chemistry.org\/prog\/peo\/."}],"container-title":["ACS Omega"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsomega.6c06743","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/acsodf\/article-pdf\/11\/39\/59807\/68632219\/acsomega.6c06743.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/acsodf\/article-pdf\/11\/39\/59807\/68632219\/acsomega.6c06743.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T15:29:28Z","timestamp":1791300568000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/acsodf\/article\/11\/39\/59807\/5437285\/Highly-Selective-Thiazole-Derivatives-as-Promising"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,24]]},"references-count":20,"journal-issue":{"issue":"39","published-online":{"date-parts":[[2026,9,24]]},"published-print":{"date-parts":[[2026,10,6]]}},"URL":"https:\/\/doi.org\/10.1021\/acsomega.6c06743","relation":{},"ISSN":["2470-1343"],"issn-type":[{"value":"2470-1343","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,24]]}}}