{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T17:03:24Z","timestamp":1790096604909,"version":"4.0.1"},"reference-count":102,"publisher":"American Chemical Society (ACS)","issue":"37","license":[{"start":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T00:00:00Z","timestamp":1789084800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100020898","name":"Universidade Federal de Ci?ncias da Sa?de de Porto Alegre","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100020898","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico","doi-asserted-by":"publisher","award":["406436\/2022-3"],"award-info":[{"award-number":["406436\/2022-3"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004263","name":"Funda??o de Amparo ? Pesquisa do Estado do Rio Grande do Sul","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004263","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,22]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Silver (AgNP) and gold (AuNP) nanoparticles are notable in nanomedicine for their unique properties and strong antibacterial effects; however, formulating them into skin-applied systems that preserve bioactivity while controlling nanoparticle exposure remains challenging. Herein, semisolid extrusion 3D printing of chitosan nanocomposite hydrogels loaded with AgNPs or AuNPs was used to manufacture personalized topical films with antimicrobial performance and minimized skin penetration risk. AgNPs and AuNPs were synthesized with comparable nanoscale dimensions (11.83 \u00b1 3.74 nm and 11.67 \u00b1 1.96 nm), enabling their uniform incorporation into chitosan matrices. The resulting inks presented topical-compatible pH (&amp;gt;4.0) and pronounced thixotropy with &amp;gt;90% viscosity recovery, translating into adequate performance in 3D printing. Nanoparticle incorporation afforded the tuning of properties such as swelling, mechanical behavior, and bioadhesion. Functionally, AgNP-loaded films exhibited the strongest broad-spectrum activity against Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans. Ex vivo porcine skin studies indicated that free nanoparticles were detectable only in the stratum corneum, whereas metals were not detected after application of the 3D-printed films, supporting effective nanoparticle confinement by the chitosan matrix. These results position 3D-printed chitosan-nanoparticle films as a promising platform for customizable antimicrobial dressings for infected skin wounds.<\/jats:p>","DOI":"10.1021\/acsomega.6c07342","type":"journal-article","created":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T17:01:43Z","timestamp":1789146103000},"page":"56346-56359","source":"Crossref","is-referenced-by-count":0,"title":["Development of Antimicrobial Chitosan-Silver and Chitosan-Gold\nNanocomposite Films by 3D Printing for Skin Application"],"prefix":"10.1021","volume":"11","author":[{"given":"Rita de Ca\u0301ssia dos Reis","family":"Schmidt","sequence":"first","affiliation":[{"name":"Universidade Federal de Cie\u0302ncias da Sau\u0301de de Porto Alegre , , Rua Sarmento Leite 245 , , ,","place":["Porto Alegre, Rio Grande do Sul, Brazil, CEP 90050-170"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vito\u0301ria Espinosa de","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Universidade Federal de Cie\u0302ncias da Sau\u0301de de Porto Alegre , Rua Sarmento Leite 245 , , ,","place":["Porto Alegre, Rio Grande do Sul, Brazil, CEP 90050-170"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taina\u0301","family":"Griebler","sequence":"additional","affiliation":[{"name":"Universidade Federal de Cie\u0302ncias da Sau\u0301de de Porto Alegre , Rua Sarmento Leite 245 , , ,","place":["Porto Alegre, Rio Grande do Sul, Brazil, CEP 90050-170"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ju\u0301lia Lazzarini","family":"Machado","sequence":"additional","affiliation":[{"name":"Universidade Federal de Cie\u0302ncias da Sau\u0301de de Porto Alegre , , Rua Sarmento Leite 245 , , ,","place":["Porto Alegre, Rio Grande do Sul, Brazil, CEP 90050-170"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo Pessoa","family":"da Silva","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rio Grande do Sul , , CP 15051 , , ,","place":["Porto Alegre, Rio Grande do Sul, Brazil, CEP 91501-970"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrique","family":"Trombini","sequence":"additional","affiliation":[{"name":"Universidade Federal de Cie\u0302ncias da Sau\u0301de de Porto Alegre , Rua Sarmento Leite 245 , , ,","place":["Porto Alegre, Rio Grande do Sul, Brazil, CEP 90050-170"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1351-2430","authenticated-orcid":true,"given":"Marcelo Barbalho","family":"Pereira","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rio Grande do Sul , , CP 15051 , , ,","place":["Porto Alegre, Rio Grande do Sul, Brazil, CEP 91501-970"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5919-8133","authenticated-orcid":true,"given":"Cesar Liberato","family":"Petzhold","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rio Grande do Sul , , CP 15003 , , ,","place":["Porto Alegre, Rio Grande do Sul, Brazil, CEP 91501-970"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabrielle Aguiar","family":"Dantas","sequence":"additional","affiliation":[{"name":"Universidade Federal de Santa Maria , , Av. Roraima 1000 , , ,","place":["Santa Maria, Rio Grande do Sul, Brazil, CEP 97105-900"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0533-3483","authenticated-orcid":true,"given":"Roberto Christ","family":"Vianna Santos","sequence":"additional","affiliation":[{"name":"Universidade Federal de Santa Maria , , Av. Roraima 1000 , , ,","place":["Santa Maria, Rio Grande do Sul, Brazil, CEP 97105-900"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadine Lysyk","family":"Funk","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rio Grande do Sul , , Av. Ipiranga 2752 , , ,","place":["Porto Alegre, Rio Grande do Sul, Brazil, CEP 90610-000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6262-1097","authenticated-orcid":true,"given":"Ruy Carlos Ruver","family":"Beck","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rio Grande do Sul , , Av. Ipiranga 2752 , , ,","place":["Porto Alegre, Rio Grande do Sul, Brazil, CEP 90610-000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1373-9336","authenticated-orcid":true,"given":"Karina","family":"Paese","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rio Grande do Sul , , Av. Ipiranga 2752 , , ,","place":["Porto Alegre, Rio Grande do Sul, Brazil, CEP 90610-000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9889-7420","authenticated-orcid":true,"given":"Monique","family":"Deon","sequence":"additional","affiliation":[{"name":"Universidade Federal de Cie\u0302ncias da Sau\u0301de de Porto Alegre , , Rua Sarmento Leite 245 , , ,","place":["Porto Alegre, Rio Grande do Sul, Brazil, CEP 90050-170"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,11]]},"reference":[{"issue":"12","key":"2026092211023503200_cit1","doi-asserted-by":"publisher","first-page":"1962","DOI":"10.3390\/polym13121962","article-title":"Biopolymer and Synthetic\nPolymer-Based Nanocomposites in Wound Dressing Applications: A Review","volume":"13","author":"Gobi","year":"2021","journal-title":"Polymers"},{"issue":"12","key":"2026092211023503200_cit2","doi-asserted-by":"publisher","first-page":"1974","DOI":"10.3390\/ijms17121974","article-title":"Future Prospects for Scaffolding Methods and Biomaterials in Skin\nTissue Engineering: A Review","volume":"17","author":"Chaudhari","year":"2016","journal-title":"Int. J. Mol. Sci."},{"key":"2026092211023503200_cit3","doi-asserted-by":"publisher","first-page":"168","DOI":"10.1016\/j.matlet.2019.01.084","article-title":"Polyvinyl alcohol\nnanofiber based three phase wound dressings for sustained wound healing\napplications","volume":"241","author":"Jatoi","year":"2019","journal-title":"Mater. Lett."},{"issue":"4","key":"2026092211023503200_cit4","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s10544-021-00581-0","article-title":"Cellulose\nnanocrystals-based materials as hemostatic agents for wound dressings:\na review","volume":"23","author":"Cidreira","year":"2021","journal-title":"Biomed. Microdevices"},{"issue":"8","key":"2026092211023503200_cit5","doi-asserted-by":"publisher","first-page":"7064","DOI":"10.1021\/acsnano.2c12488","article-title":"Review of Antimicrobial\nNanocoatings in Medicine and Dentistry: Mechanisms of Action, Biocompatibility\nPerformance, Safety, and Benefits Compared to Antibiotics","volume":"17","author":"Butler","year":"2023","journal-title":"ACS Nano"},{"key":"2026092211023503200_cit6","doi-asserted-by":"publisher","first-page":"3030","DOI":"10.1021\/acsanm.1c03891","article-title":"Synergistic Effects Between Metal\nNanoparticles and Commercial Antimicrobial Agents: A Review","volume":"5","author":"Ribeiro","year":"2022","journal-title":"ACS Appl. Nano Mater."},{"issue":"34","key":"2026092211023503200_cit7","doi-asserted-by":"publisher","first-page":"40241","DOI":"10.1021\/acsami.3c07625","article-title":"Multifunctional Gold\u2013Silver\u2013Carbon\nQuantum Dots Nano-Hybrid Composite: Advancing Antibacterial Wound\nHealing and Cell Proliferation","volume":"15","author":"Wang","year":"2023","journal-title":"ACS Appl. Mater.\nInterfaces"},{"key":"2026092211023503200_cit8","doi-asserted-by":"publisher","first-page":"114605","DOI":"10.1016\/J.COLSURFB.2025.114605","article-title":"Polymeric nanocomposites\nin a biological interface: From a molecular view to final applications","volume":"251","author":"dos Reis Schmidt","year":"2025","journal-title":"Colloids Surf., B"},{"key":"2026092211023503200_cit9","doi-asserted-by":"publisher","first-page":"1264","DOI":"10.3390\/antibiotics12081264","article-title":"Nanosilver: An Old Antibacterial\nAgent with Great Promise in the Fight against Antibiotic Resistance","volume":"12","author":"Kaiser","year":"2023","journal-title":"Antibiotics"},{"key":"2026092211023503200_cit10","doi-asserted-by":"publisher","first-page":"100804","DOI":"10.1016\/j.colcom.2024.100804","article-title":"Gold nanoparticles\nantibacterial activity: Does the surface matter?","volume":"62","author":"Sa\u2019ed","year":"2024","journal-title":"Colloid. Interface Sci. Commun."},{"key":"2026092211023503200_cit11","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1021\/acs.chemrestox.3c00154","article-title":"Silver\nNanoparticles Induced Metabolic Perturbations\nin Pseudomonas aeruginosa: Evaluation Using the UPLC-QTof-MSE Platform","volume":"37","author":"Konda","year":"2024","journal-title":"Chem. Res. Toxicol."},{"key":"2026092211023503200_cit12","doi-asserted-by":"publisher","first-page":"4162","DOI":"10.1021\/acsabm.4c00476","article-title":"Silver and Copper Nanoparticle-Loaded\nSelf-Assembled Pseudo-Peptide\nThiourea-Based Organic-Inorganic Hybrid Gel with Antibacterial and\nSuperhydrophobic Properties for Antifouling Surfaces","volume":"7","author":"Devi","year":"2024","journal-title":"ACS Appl. Bio Mater."},{"issue":"3","key":"2026092211023503200_cit13","doi-asserted-by":"publisher","first-page":"679","DOI":"10.3390\/biomedicines10030679","article-title":"Benefit of Silver and\nGold Nanoparticles in Wound Healing Process after Endometrial Cancer\nProtocol","volume":"10","author":"Toczek","year":"2022","journal-title":"Biomedicines"},{"issue":"1","key":"2026092211023503200_cit14","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1080\/20550324.2022.2091340","article-title":"Study on green preparation\nof multi-branched gold nanoparticles loaded flexible polyurethane\nfoam for antibacterial dressing","volume":"8","author":"Huynh","year":"2022","journal-title":"Nanocomposites"},{"key":"2026092211023503200_cit15","doi-asserted-by":"publisher","first-page":"21997","DOI":"10.1038\/s41598-024-71781-w","article-title":"Effect\nof silver nanoparticles and\nREP-PCR typing of Staphylococcus aureus isolated from various sources","volume":"14","author":"Elghazaly","year":"2024","journal-title":"Sci. Rep."},{"key":"2026092211023503200_cit16","doi-asserted-by":"publisher","first-page":"9873","DOI":"10.1021\/acsomega.2c06789","article-title":"Inhibition of Biofilm\nand Virulence Properties of Pathogenic\nBacteria by Silver and Gold Nanoparticles Synthesized from Lactiplantibacillus\nsp. Strain C1","volume":"8","author":"Kang","year":"2023","journal-title":"ACS Omega"},{"key":"2026092211023503200_cit17","doi-asserted-by":"publisher","first-page":"647","DOI":"10.1021\/acsbiomaterials.7b00511","article-title":"High Antifungal\nActivity against Candida Species of\nMonometallic and Bimetallic Nanoparticles Synthesized in Nanoreactors","volume":"4","author":"Gutie\u0301rrez","year":"2018","journal-title":"ACS Biomater. Sci. Eng."},{"issue":"20","key":"2026092211023503200_cit18","doi-asserted-by":"publisher","first-page":"16834","DOI":"10.1021\/acsami.7b02775","article-title":"Functional Silver Nanocomposites\nas Broad-Spectrum Antimicrobial and Biofilm-Disrupting Agents","volume":"9","author":"Guo","year":"2017","journal-title":"ACS Appl. Mater. Interfaces"},{"issue":"3","key":"2026092211023503200_cit19","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1134\/S1062359014030042","article-title":"Bioaccumulation of silver and gold\nnanoparticles in organs and tissues of rats studied by neutron activation\nanalysis","volume":"41","author":"Buzulukov","year":"2014","journal-title":"Biol. Bull."},{"issue":"2","key":"2026092211023503200_cit20","doi-asserted-by":"publisher","first-page":"146","DOI":"10.1016\/j.jdermsci.2017.11.001","article-title":"Penetration\nof gold nanoparticles across the stratum corneum layer of thick-Skin","volume":"89","author":"Raju","year":"2018","journal-title":"J. Dermatol. Sci."},{"issue":"1","key":"2026092211023503200_cit21","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/srep16908","article-title":"Shape-Dependent\nSkin Penetration of Silver Nanoparticles: Does It Really Matter?","volume":"5","author":"Tak","year":"2015","journal-title":"Sci. Rep."},{"issue":"2","key":"2026092211023503200_cit22","doi-asserted-by":"publisher","first-page":"470","DOI":"10.3390\/polym12020470","article-title":"Role of Nanoparticle\u2013Polymer\nInteractions on the Development of Double-Network Hydrogel Nanocomposites\nwith High Mechanical Strength","volume":"12","author":"Chang","year":"2020","journal-title":"Polymers"},{"key":"2026092211023503200_cit23","doi-asserted-by":"publisher","first-page":"103","DOI":"10.34172\/japid.026.3939","article-title":"Development\nand evaluation of a PLGA\/TiO2 nanocomposite for periodontal applications:\nSynthesis, characterization, and cytocompatibility with human gingival\nfibroblasts-An in vitro study","volume":"18","author":"Kashefimehr","year":"2026","journal-title":"J. Adv. Periodontol.\nImplant Dent."},{"key":"2026092211023503200_cit24","doi-asserted-by":"publisher","first-page":"116580","DOI":"10.1016\/j.envres.2023.116580","article-title":"Sustainable functionalized chitosan based nano-composites\nfor wound dressings applications: A review","volume":"235","author":"Verma","year":"2023","journal-title":"Environ. Res."},{"key":"2026092211023503200_cit25","doi-asserted-by":"publisher","first-page":"460","DOI":"10.1016\/j.carbpol.2016.09.051","article-title":"Enhanced\nantibacterial and wound healing activities of microporous\nchitosan-Ag\/ZnO composite dressing","volume":"156","author":"Lu","year":"2017","journal-title":"Carbohydr.\nPolym."},{"key":"2026092211023503200_cit26","doi-asserted-by":"publisher","first-page":"441","DOI":"10.1016\/j.jiec.2024.11.029","article-title":"Synthesis and Comprehensive Characterization\nof PVA\/Chitosan\/Cu Nanowires Wound Dressing Hydrogel for Effective\nIn Vitro Wound Healing","volume":"146","author":"Jallab","year":"2025","journal-title":"J. Ind. Eng. Chem."},{"key":"2026092211023503200_cit27","doi-asserted-by":"publisher","first-page":"360","DOI":"10.1016\/j.msec.2019.03.092","article-title":"A novel bioactive\nquaternized chitosan and its silver-containing nanocomposites as a\npotent antimicrobial wound dressing: Structural and biological properties","volume":"101","author":"Rahimi","year":"2019","journal-title":"Mater. Sci. Eng."},{"key":"2026092211023503200_cit28","doi-asserted-by":"publisher","first-page":"104545","DOI":"10.1016\/J.MTCOMM.2022.104545","article-title":"Novel dual-ionic\nZnO\/CuO embedded in porous chitosan biopolymer for wound dressing\napplication: Physicochemical, bactericidal, cytocompatibility and\nwound healing profiles","volume":"33","author":"Govindasamy","year":"2022","journal-title":"Mater. Today Commun."},{"issue":"3","key":"2026092211023503200_cit29","doi-asserted-by":"publisher","first-page":"267","DOI":"10.1016\/j.bioactmat.2017.11.003","article-title":"Chitosan based\nmetallic nanocomposite scaffolds as antimicrobial wound dressings","volume":"3","author":"Mohandas","year":"2018","journal-title":"Bioact. Mater."},{"key":"2026092211023503200_cit30","doi-asserted-by":"publisher","first-page":"481","DOI":"10.1016\/j.ijbiomac.2021.02.045","article-title":"Ag@MOF-loaded\nchitosan nanoparticle and polyvinyl alcohol\/sodium\nalginate\/chitosan bilayer dressing for wound healing applications","volume":"175","author":"Zhang","year":"2021","journal-title":"Int. J. Biol. Macromol."},{"key":"2026092211023503200_cit31","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1007\/978-81-322-2511-9_1","article-title":"Chitosan Hydrogels\nfor Regenerative Engineering","author":"Padmanabhan","year":"2016","journal-title":"Chitin Chitosan\nRegener. Med."},{"issue":"4","key":"2026092211023503200_cit32","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2023.e14682","article-title":"3D printed superparamagnetic\nstimuli-responsive starfish-shaped hydrogels","volume":"9","author":"Mohammed","year":"2023","journal-title":"Heliyon"},{"key":"2026092211023503200_cit33","doi-asserted-by":"publisher","first-page":"112841","DOI":"10.1016\/J.EURPOLYMJ.2024.112841","article-title":"Modification\nof agar hydrogels for additive 3D printing technologies","volume":"210","author":"Zakharova","year":"2024","journal-title":"Eur. Polym. J."},{"key":"2026092211023503200_cit34","doi-asserted-by":"publisher","first-page":"41107","DOI":"10.1021\/acsomega.4c04961","article-title":"Silver Nanoparticles\nin 3D Printing: A New Frontier\nin Wound Healing","volume":"9","author":"Astaneh","year":"2024","journal-title":"ACS Omega"},{"key":"2026092211023503200_cit35","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1007\/978-981-99-2404-2_5","article-title":"Semi-Solid Extrusion\n(SSE) in Pharmaceuticals","author":"Funk","year":"2023","journal-title":"Addit. Manuf. Pharm."},{"key":"2026092211023503200_cit36","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/B978-0-12-815890-6.00001-3","article-title":"printing in\nmedicine: Current challenges\nand potential applications","author":"Ashish","year":"2019","journal-title":"3D Print. Technol.\nNanomed."},{"key":"2026092211023503200_cit37","doi-asserted-by":"publisher","DOI":"10.3390\/gels12060501","volume-title":"Multifunctional Hydrogel-Based\nScaffolds:\nintegrating Conductive Nanomaterials for Smart Wound Healing Applications","author":"Jeon","year":"2026"},{"key":"2026092211023503200_cit38","doi-asserted-by":"publisher","first-page":"133989","DOI":"10.1016\/j.ijbiomac.2024.133989","article-title":"Synergistic effect of Cydonia oblonga and its\nextracted silver nanoparticles for improving antioxidant and antibacterial\nactivity of 3D printed alginate-based hydrogel as wound dressing","volume":"276","author":"Ashtariyan","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"2026092211023503200_cit39","doi-asserted-by":"publisher","first-page":"1104","DOI":"10.3390\/antibiotics12071104","article-title":"Rapid Prototyping of 3D-Printed AgNPs- and Nano-TiO2-Embedded\nHydrogels as Novel Devices with Multiresponsive Antimicrobial Capability\nin Wound Healing","volume":"12","author":"Remaggi","year":"2023","journal-title":"Antibiotics"},{"key":"2026092211023503200_cit40","doi-asserted-by":"publisher","first-page":"2954","DOI":"10.1039\/C8TB03341B","article-title":"A skin-inspired 3D\nbilayer scaffold enhances granulation tissue formation and anti-infection\nfor diabetic wound healing","volume":"7","author":"Wan","year":"2019","journal-title":"J. Mater. Chem.\nB"},{"key":"2026092211023503200_cit41","doi-asserted-by":"publisher","first-page":"12375","DOI":"10.1021\/acsnano.1c04499","article-title":"Multifunctional\n3D-Printed\nWound Dressings","volume":"15","author":"Alizadehgiashi","year":"2021","journal-title":"ACS Nano"},{"key":"2026092211023503200_cit42","doi-asserted-by":"publisher","first-page":"1089","DOI":"10.3390\/jpm12071089","article-title":"Hemostasis and Anti-Inflammatory\nAbilities of AuNPs-Coated Chitosan Dressing for Burn Wounds","volume":"12","author":"Cherng","year":"2022","journal-title":"J. Pers. Med."},{"key":"2026092211023503200_cit43","doi-asserted-by":"publisher","first-page":"174","DOI":"10.1016\/j.ijpharm.2019.04.079","article-title":"Gold nanoparticles\nloaded into polymeric hydrogel for\nwound healing in rats: Effect of nanoparticles\u2019 shape and surface\nmodification","volume":"565","author":"Mahmoud","year":"2019","journal-title":"Int. J. Pharm."},{"key":"2026092211023503200_cit44","doi-asserted-by":"publisher","first-page":"152645","DOI":"10.1016\/j.ijbiomac.2026.152645","article-title":"Melatonin-loaded 3D-printed carboxymethyl\ncellulose-gold\nnanoparticles composite hydrogels for wound healing","volume":"369","author":"Zarur","year":"2026","journal-title":"Int. J. Biol. Macromol."},{"key":"2026092211023503200_cit45","doi-asserted-by":"publisher","first-page":"543419","DOI":"10.1155\/2014\/543419","article-title":"Characterization and biocompatibility of chitosan gels with silver\nand gold nanoparticles","volume":"2014","author":"Sa\u0301mano-Valencia","year":"2014","journal-title":"J. Nanomater."},{"issue":"1","key":"2026092211023503200_cit46","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1007\/s10971-018-4699-6","article-title":"Chitosan-stabilized\ngold nanoparticles supported on silica\/titania magnetic xerogel applied\nas antibacterial system","volume":"89","author":"Deon","year":"2019","journal-title":"J. Sol-Gel Sci. Technol."},{"issue":"16","key":"2026092211023503200_cit47","doi-asserted-by":"publisher","first-page":"23962","DOI":"10.1021\/acsomega.5c11817","article-title":"Semi-solid Extrusion 3D Printing of Chitosan\/Carbon\nNanotube Nanocomposite Films for Microextraction of Pesticides in\nWater","volume":"11","author":"Salvador","year":"2026","journal-title":"ACS Omega"},{"issue":"5","key":"2026092211023503200_cit48","doi-asserted-by":"publisher","first-page":"1412","DOI":"10.1007\/s10924-020-01959-6","article-title":"Green Synthesis\nof\nSilver Nanoparticles Using Nymphae odorata Extract Incorporated Films\nand Antimicrobial Activity","volume":"29","author":"Gudimalla","year":"2021","journal-title":"J. Polym. Environ."},{"issue":"27","key":"2026092211023503200_cit49","doi-asserted-by":"publisher","first-page":"5732","DOI":"10.1002\/adma.201504829","article-title":"New Developments in\nSmart Bandage Technologies for Wound Diagnostics","volume":"28","author":"McLister","year":"2016","journal-title":"Adv. Mater."},{"issue":"2","key":"2026092211023503200_cit50","doi-asserted-by":"publisher","first-page":"88","DOI":"10.12968\/jowc.2012.21.2.88","article-title":"The effect of wound dressings on\nthe pH stability of fluids","volume":"21","author":"Uzun","year":"2012","journal-title":"J. Wound Care"},{"issue":"7","key":"2026092211023503200_cit51","doi-asserted-by":"publisher","first-page":"200","DOI":"10.1208\/s12249-025-03197-2","article-title":"Fast-Dissolving Pullulan Film as a Solid Dosage\nForm for Sublingual Administration of Nifedipine-Loaded Nanocapsules","volume":"26","author":"Osmari","year":"2025","journal-title":"AAPS PharmSciTech"},{"issue":"9","key":"2026092211023503200_cit52","doi-asserted-by":"publisher","first-page":"1253","DOI":"10.1039\/c3pp50373a","article-title":"Penetration, photo-reactivity\nand photoprotective\nproperties of nanosized ZnO","volume":"13","author":"Detoni","year":"2014","journal-title":"Photochem. Photobiol.\nSci."},{"issue":"5","key":"2026092211023503200_cit53","doi-asserted-by":"publisher","first-page":"2521","DOI":"10.1021\/bm0502258","article-title":"Preparation and characterization\nof chitosan-based nanoparticles","volume":"6","author":"Bodnar","year":"2005","journal-title":"Biomacromolecules"},{"issue":"2","key":"2026092211023503200_cit54","doi-asserted-by":"publisher","first-page":"1402","DOI":"10.1016\/j.carbpol.2012.10.008","article-title":"Stabilisation\nof silver and copper nanoparticles in a chemically modified chitosan\nmatrix","volume":"92","author":"Tiwari","year":"2013","journal-title":"Carbohydr. Polym."},{"issue":"8","key":"2026092211023503200_cit55","doi-asserted-by":"publisher","first-page":"1237","DOI":"10.5935\/0103-5053.20130157","article-title":"Nano-assembled supramolecular films from chitosan-stabilized\ngold nanoparticles and cobalt(II) phthalocyanine","volume":"24","author":"Silva","year":"2013","journal-title":"J. Braz. Chem. Soc."},{"issue":"12","key":"2026092211023503200_cit56","doi-asserted-by":"publisher","first-page":"2642","DOI":"10.21577\/0103-5053.20190182","article-title":"Photochemical\nSynthesis\nof Ag and Au Nanoparticles Using a Thioxanthone Substituted Chitosan\nas Simultaneous Photoinitiator and Stabilizer","volume":"30","author":"Valandro","year":"2019","journal-title":"J. Braz Chem. Soc."},{"issue":"9","key":"2026092211023503200_cit57","doi-asserted-by":"publisher","first-page":"3747","DOI":"10.1007\/s10854-014-2085-x","article-title":"Structural, thermal,\nzeta potential\nand electrical properties of disaccharide reduced silver nanoparticles","volume":"25","author":"Singh","year":"2014","journal-title":"J. Mater. Sci.: mater. Electron"},{"key":"2026092211023503200_cit58","doi-asserted-by":"publisher","first-page":"3647","DOI":"10.1016\/j.matpr.2020.10.681","article-title":"A study\non the green synthesis of\nsilver nanoparticles from Olea europaea and its activity against oral\npathogens","volume":"44","author":"Umai","year":"2021","journal-title":"Mater. Today Proc."},{"issue":"1","key":"2026092211023503200_cit59","doi-asserted-by":"publisher","first-page":"258","DOI":"10.1186\/s11671-019-3083-y","article-title":"Gold nanoparticles with chitosan,\nN-acylated chitosan, and chitosan oligosaccharide as DNA carriers","volume":"14","author":"Abrica-Gonza\u0301lez","year":"2019","journal-title":"Nanoscale Res. Lett."},{"key":"2026092211023503200_cit60","doi-asserted-by":"publisher","first-page":"116983","DOI":"10.1016\/j.carbpol.2020.116983","article-title":"Rapid synthesis of chitosan-capped\ngold nanoparticles for analytical application and facile recovery\nof gold from laboratory waste","volume":"250","author":"Thanayutsiri","year":"2020","journal-title":"Carbohydr. Polym."},{"key":"2026092211023503200_cit61","doi-asserted-by":"publisher","first-page":"120054","DOI":"10.1016\/j.carbpol.2022.120054","article-title":"High strength chitosan\nhydrogels prepared from NaOH\/urea aqueous solutions: the role of thermal\ngelling","volume":"297","author":"Lu","year":"2022","journal-title":"Carbohydr. Polym."},{"key":"2026092211023503200_cit62","doi-asserted-by":"publisher","first-page":"904","DOI":"10.3390\/polym13060904","article-title":"Antimicrobial\nActions\nand Applications of Chitosan","volume":"13","author":"Ke","year":"2021","journal-title":"Polymers"},{"issue":"3","key":"2026092211023503200_cit63","doi-asserted-by":"publisher","first-page":"69","DOI":"10.3390\/cosmetics8030069","article-title":"Towards Optimal pH of the Skin and\nTopical Formulations: From the Current State of the Art to Tailored\nProducts","volume":"8","author":"Lukic\u0301","year":"2021","journal-title":"Cosmetics"},{"issue":"6","key":"2026092211023503200_cit64","doi-asserted-by":"publisher","first-page":"457","DOI":"10.1111\/1346-8138.14891","article-title":"Skin acidification with\na water-in-oil emulsion (pH 4) restores disrupted epidermal barrier\nand improves structure of lipid lamellae in the elderly","volume":"46","author":"Kilic","year":"2019","journal-title":"J. Dermatol."},{"key":"2026092211023503200_cit65","doi-asserted-by":"publisher","first-page":"174","DOI":"10.1111\/wrr.12125","article-title":"The effects of pH\non wound healing, biofilms, and antimicrobial efficacy","volume":"22","author":"Percival","year":"2014","journal-title":"Wound Repair Regener."},{"key":"2026092211023503200_cit66","doi-asserted-by":"publisher","first-page":"109777","DOI":"10.1016\/j.eurpolymj.2020.109777","article-title":"Antibacterial\nnano cerium oxide\/chitosan\/cellulose acetate composite films as potential\nwound dressing","volume":"133","author":"Kalayc\u0131og\u0306lu","year":"2020","journal-title":"Eur. Polym. J."},{"key":"2026092211023503200_cit67","doi-asserted-by":"publisher","first-page":"103095","DOI":"10.1016\/j.cis.2024.103095","article-title":"Innovations in hydrogel-based manufacturing:\nA comprehensive review of direct ink writing technique for biomedical\napplications","volume":"324","author":"Baniasadi","year":"2024","journal-title":"Adv. Colloid Interface Sci."},{"key":"2026092211023503200_cit68","doi-asserted-by":"publisher","first-page":"119983","DOI":"10.1016\/j.ijpharm.2020.119983","article-title":"3D printing of extended-release\ntablets of theophylline using hydroxypropyl methylcellulose (HPMC)\nhydrogels","volume":"591","author":"Cheng","year":"2020","journal-title":"Int. J. Pharm."},{"key":"2026092211023503200_cit69","doi-asserted-by":"publisher","first-page":"124017","DOI":"10.1016\/j.ijpharm.2024.124017","article-title":"Pimobendan controlled release guar\ngum printlets: Tailoring\ndrug doses for personalised veterinary medicines","volume":"655","author":"Lea\u0303o","year":"2024","journal-title":"Int. J. Pharm."},{"issue":"12","key":"2026092211023503200_cit70","doi-asserted-by":"publisher","first-page":"3383","DOI":"10.1021\/bm800793z","article-title":"Collagen Crosslinking\nwith Au Nanoparticles","volume":"9","author":"Castaneda","year":"2008","journal-title":"Biomacromolecules"},{"issue":"1","key":"2026092211023503200_cit71","doi-asserted-by":"publisher","first-page":"32","DOI":"10.66617\/001c.1001","article-title":"Investigation\nof thermal and viscoelastic properties of polymers relevant to hot\nmelt extrusion - I: Polyvinylpyrrolidone and related polymers","volume":"5","author":"Gupta","year":"2016","journal-title":"Int. J. Pharm. Excipients"},{"issue":"12","key":"2026092211023503200_cit72","doi-asserted-by":"publisher","first-page":"4868","DOI":"10.3390\/molecules28124868","article-title":"Understanding Gel-Powers:\nExploring Rheological Marvels of Acrylamide\/Sodium Alginate Double-Network\nHydrogels","volume":"28","author":"Wang","year":"2023","journal-title":"Molecules"},{"issue":"6","key":"2026092211023503200_cit73","doi-asserted-by":"publisher","first-page":"2017","DOI":"10.1039\/c2sm26878g","article-title":"Supramolecular\nhydrogels for creating gold and silver nanoparticles in situ","volume":"9","author":"Shen","year":"2013","journal-title":"Soft Matter"},{"issue":"11","key":"2026092211023503200_cit74","doi-asserted-by":"publisher","first-page":"1665","DOI":"10.1080\/17425247.2024.2399706","article-title":"3D printed personalized therapies for pediatric patients\naffected by adrenal insufficiency","volume":"21","author":"Rodri\u0301guez-Pombo","year":"2024","journal-title":"Expert Opin.\nDrug Deliv."},{"issue":"11","key":"2026092211023503200_cit75","doi-asserted-by":"publisher","first-page":"1680","DOI":"10.3390\/polym13111680","article-title":"Chitosan\/gelatin\/silver nanoparticles\ncomposites films for biodegradable food packaging applications","volume":"13","author":"Ediyilyam","year":"2021","journal-title":"Polymers"},{"issue":"6","key":"2026092211023503200_cit76","doi-asserted-by":"publisher","first-page":"2358","DOI":"10.1021\/acsbiomaterials.1c00441","article-title":"Gold, Silver,\nand Iron Oxide Nanoparticle Incorporation into Silk Hydrogels for\nBiomedical Applications: Elaboration, Structure, and Properties","volume":"7","author":"Belda Mari\u0301n","year":"2021","journal-title":"ACS Biomater. Sci. Eng."},{"key":"2026092211023503200_cit77","doi-asserted-by":"publisher","first-page":"325","DOI":"10.1016\/j.colsurfb.2017.09.044","article-title":"Preparation\nand cytotoxicity of chitosan-based\nhydrogels modified with silver nanoparticles","volume":"160","author":"Tyliszczak","year":"2017","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"2026092211023503200_cit78","doi-asserted-by":"publisher","first-page":"1539","DOI":"10.2147\/IJN.S448282","article-title":"Hydrogels Containing\nChitosan-Modified Gold Nanoparticles\nShow Significant Efficacy in Healing Diabetic Wounds Infected with\nAntibiotic-Resistant Bacteria","volume":"19","author":"Meng","year":"2024","journal-title":"Int. J. Nanomed."},{"issue":"8","key":"2026092211023503200_cit79","doi-asserted-by":"publisher","first-page":"2892","DOI":"10.1002\/jps.21210","article-title":"Wound Healing\nDressings and Drug Delivery Systems: A Review","volume":"97","author":"Boateng","year":"2008","journal-title":"J. Pharm. Sci."},{"key":"2026092211023503200_cit80","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1002\/marc.201800337","article-title":"Nanoparticle\u2013Polymer\nSynergies in Nanocomposite Hydrogels: From Design to Application","volume":"39","author":"Chen","year":"2018","journal-title":"Macromol. Rapid Commun."},{"key":"2026092211023503200_cit81","doi-asserted-by":"publisher","first-page":"116063","DOI":"10.1016\/j.carbpol.2020.116063","article-title":"Controlled size green\nsynthesis of bioactive silver nanoparticles assisted by chitosan and\nits derivatives and their application in biofilm preparation","volume":"236","author":"Affes","year":"2020","journal-title":"Carbohydr. Polym."},{"key":"2026092211023503200_cit82","doi-asserted-by":"publisher","first-page":"125118","DOI":"10.1016\/j.ijbiomac.2023.125118","article-title":"Green synthesis\nof nano silver-embedded\ncarboxymethyl starch waste\/poly vinyl alcohol hydrogel with photothermal\nsterilization and pH-responsive behavior","volume":"242","author":"Srikhao","year":"2023","journal-title":"Int.\nJ. Biol. Macromol"},{"key":"2026092211023503200_cit83","doi-asserted-by":"publisher","first-page":"838","DOI":"10.1016\/j.ijbiomac.2018.11.231","article-title":"One-pot synthesis of gold nanoparticles\nembedded in polysaccharide-based hydrogel: Physical-chemical characterization\nand feasibility for large-scale production","volume":"124","author":"Marques","year":"2019","journal-title":"Int. J. Biol. Macromol."},{"key":"2026092211023503200_cit84","doi-asserted-by":"publisher","first-page":"46202","DOI":"10.1021\/acsami.1c15948","article-title":"Dual Functions\nof a Au@AgNP-Incorporated Nanocomposite\nDesalination Membrane with an Enhanced Antifouling Property and Fouling\nDetection Via Surface-Enhanced Raman Spectroscopy","volume":"13","author":"Zhang","year":"2021","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"2026092211023503200_cit85","doi-asserted-by":"publisher","first-page":"1136077","DOI":"10.3389\/fbioe.2023.1136077","article-title":"Polymeric biomaterials for wound\nhealing","volume":"11","author":"Oliveira","year":"2023","journal-title":"Front. Bioeng. Biotechnol."},{"issue":"9","key":"2026092211023503200_cit86","doi-asserted-by":"publisher","first-page":"413","DOI":"10.1007\/s00403-006-0713-x","article-title":"Influence\nof pH on wound-healing: A new perspective for wound-therapy?","volume":"298","author":"Schneider","year":"2007","journal-title":"Arch. Dermatol. Res."},{"key":"2026092211023503200_cit87","doi-asserted-by":"publisher","first-page":"127053","DOI":"10.1016\/j.ijbiomac.2023.127053","article-title":"Hydrogen sulfide\nreleasing poly(\u03b3-glutamic acid) biocomposite hydrogel with monitoring,\nantioxidant, and antibacterial properties for diabetic wound healing","volume":"253","author":"Liu","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"issue":"4","key":"2026092211023503200_cit88","doi-asserted-by":"publisher","first-page":"675","DOI":"10.3390\/polym11040675","article-title":"The Effect of Nanofillers on the\nFunctional Properties of Biopolymer-Based Films: A Review","volume":"11","author":"Jamro\u0301z","year":"2019","journal-title":"Polymers"},{"issue":"5","key":"2026092211023503200_cit89","doi-asserted-by":"publisher","first-page":"721","DOI":"10.3390\/POLYM13050721","article-title":"Understanding\nthe Barrier and Mechanical Behavior of Different Nanofillers in Chitosan\nFilms for Food Packaging","volume":"13","author":"Pires","year":"2021","journal-title":"Polymers"},{"key":"2026092211023503200_cit90","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1016\/j.bioactmat.2021.11.008","article-title":"Cohesion mechanisms for bioadhesives","volume":"13","author":"Bu","year":"2022","journal-title":"Bioact. Mater."},{"key":"2026092211023503200_cit91","doi-asserted-by":"publisher","first-page":"357","DOI":"10.1016\/j.ijid.2020.10.092","article-title":"Candida colonization\nas a predictor of invasive candidiasis in non-neutropenic ICU patients\nwith sepsis: A systematic review and meta-analysis","volume":"102","author":"Alenazy","year":"2021","journal-title":"Int. J. Infect. Dis."},{"issue":"8","key":"2026092211023503200_cit92","doi-asserted-by":"publisher","first-page":"2818","DOI":"10.1073\/pnas.0813394106","article-title":"Harnessing\nHsp90 function as a powerful, broadly\neffective therapeutic strategy for fungal infectious disease","volume":"106","author":"Cowen","year":"2009","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"2026092211023503200_cit93","doi-asserted-by":"publisher","first-page":"S612","DOI":"10.1093\/CID\/CIV791","article-title":"Echinocandin\nResistance in Candida","volume":"61","author":"Perlin","year":"2015","journal-title":"Clin. Infect. Dis."},{"key":"2026092211023503200_cit94","doi-asserted-by":"publisher","DOI":"10.1002\/slct.202404909","article-title":"Synthesis and Characterization of Chitosan\u2013Silver Nanocomposite\nFilm: Antibacterial and Cytotoxicity Study","volume":"9","author":"Ogungbesan","year":"2024","journal-title":"ChemistrySelect"},{"issue":"2","key":"2026092211023503200_cit95","doi-asserted-by":"publisher","first-page":"1087","DOI":"10.1021\/am508094e","article-title":"Development\nof Noncytotoxic Chitosan\u2013Gold Nanocompositesas Efficient Antibacterial\nMaterials","volume":"7","author":"Regiel-Futyra","year":"2015","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"2026092211023503200_cit96","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1016\/j.ijbiomac.2014.05.047","article-title":"Chitosan nanocomposite films based\non Ag-NP and Au-NP biosynthesis by Bacillus Subtilis as packaging\nmaterials","volume":"69","author":"Youssef","year":"2014","journal-title":"Int. J. Biol. Macromol."},{"issue":"6","key":"2026092211023503200_cit97","doi-asserted-by":"publisher","first-page":"850","DOI":"10.1049\/iet-nbt.2017.0311","article-title":"Eco-friendly\nsynthesis of silver\nand gold nanoparticles with enhanced antimicrobial, antioxidant, and\ncatalytic activities","volume":"12","author":"Vijayan","year":"2018","journal-title":"IET Nanobiotechnol."},{"issue":"21","key":"2026092211023503200_cit98","doi-asserted-by":"publisher","first-page":"17693","DOI":"10.1021\/acsami.6b15123","article-title":"Bactericidal Effect\nof Gold\u2013Chitosan Nanocomposites in Coculture Models of Pathogenic\nBacteria and Human Macrophages","volume":"9","author":"Mendoza","year":"2017","journal-title":"ACS Appl. Mater.\nInterfaces"},{"issue":"1","key":"2026092211023503200_cit99","doi-asserted-by":"publisher","first-page":"260","DOI":"10.1021\/jf304006y","article-title":"Silver ions\nrelease from antibacterial chitosan films containing in situ generated\nsilver nanoparticles","volume":"61","author":"Lo\u0301pez-Carballo","year":"2013","journal-title":"J. Agric. Food Chem."},{"issue":"4","key":"2026092211023503200_cit100","doi-asserted-by":"publisher","first-page":"427","DOI":"10.1007\/s12088-017-0670-4","article-title":"Preparation, Characterization,\nand Antimicrobial Properties of Chitosan\u2013Silver Nanocomposites\nFilms Against Fish Pathogenic Bacteria and Fungi","volume":"57","author":"Dananjaya","year":"2017","journal-title":"Indian J. Microbiol."},{"issue":"16","key":"2026092211023503200_cit101","doi-asserted-by":"publisher","first-page":"8655","DOI":"10.1007\/s10570-019-02678-5","article-title":"Laccase\/TEMPO-mediated bacterial cellulose functionalization:\nproduction of paper-silver nanoparticles composite with antimicrobial\nactivity","volume":"26","author":"Morena","year":"2019","journal-title":"Cellulose"},{"issue":"23","key":"2026092211023503200_cit102","doi-asserted-by":"publisher","first-page":"6325","DOI":"10.1007\/s10853-009-3871-3","article-title":"Nanostructure characterization of polymer-stabilized\ngold nanoparticles and nanofilms derived from green synthesis","volume":"44","author":"Medina-Rami\u0301rez","year":"2009","journal-title":"J. Mater. Sci."}],"container-title":["ACS Omega"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsomega.6c07342","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/acsodf\/article-pdf\/11\/37\/56346\/68487893\/acsomega.6c07342.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/acsodf\/article-pdf\/11\/37\/56346\/68487893\/acsomega.6c07342.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T16:03:35Z","timestamp":1790093015000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/acsodf\/article\/11\/37\/56346\/5428659\/Development-of-Antimicrobial-Chitosan-Silver-and"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,11]]},"references-count":102,"journal-issue":{"issue":"37","published-online":{"date-parts":[[2026,9,11]]},"published-print":{"date-parts":[[2026,9,22]]}},"URL":"https:\/\/doi.org\/10.1021\/acsomega.6c07342","relation":{},"ISSN":["2470-1343"],"issn-type":[{"value":"2470-1343","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,11]]}}}