{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T15:58:16Z","timestamp":1791302296936,"version":"4.1.0"},"reference-count":50,"publisher":"American Chemical Society (ACS)","issue":"39","license":[{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001412","name":"Council of Scientific and Industrial Research, India","doi-asserted-by":"publisher","award":["27(0325)\/17\/EMR-II"],"award-info":[{"award-number":["27(0325)\/17\/EMR-II"]}],"id":[{"id":"10.13039\/501100001412","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001407","name":"Department of Biotechnology, Ministry of Science and Technology, India","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100021111","name":"Indian Institute of Science Education and Research Tirupati","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100021111","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Biofilm represents a complex aggregation of bacteria embedded within a self-produced extracellular polymeric substance (EPS). We investigated the characteristics of mycobacterial biofilm using Mycobacterium smegmatis (Msm) as a model organism. By combining transcriptomic (RNA-seq) and proteomic (LC-MS) analyses, the research captures dynamic changes during the establishment and maturation of the biofilm. Transcriptomics analysis showed a distinct gene expression profile as compared to its planktonic form. Interestingly, clear differences were seen between initial (\u223c2-day old) and mature (\u223c5-day old) biofilm stages, highlighting phasic gene expression throughout biofilm development. Marked alteration in oxidative stress-related genes and energy metabolism from ATP to NADH was observed. Furthermore, quantitative mass spectrometry-based proteome examination of EPS showed an abundance of cytoplasmic proteins present differentially between initial and mature biofilm stages. Pathway enrichment revealed enhanced oxidative stress responses and metabolic shifts in mature biofilms, including upregulation of NADH dehydrogenase and downregulation of ATP synthase, indicating altered energy metabolism. Our findings thus provide insights into the molecular adaptations, including production of mycofactocin, occurring during mycobacterial biofilm establishment and maturation, and advance our understanding of mycobacterial biofilm physiology.<\/jats:p>","DOI":"10.1021\/acsomega.6c07807","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T14:04:34Z","timestamp":1789999474000},"page":"60223-60233","source":"Crossref","is-referenced-by-count":0,"title":["From Planktonic\nto Sedentary Lifestyle: Molecular\nDissection of the Establishment and Maintenance of Mycobacterial Biofilm"],"prefix":"10.1021","volume":"11","author":[{"given":"Himalaya","family":"Naik","sequence":"first","affiliation":[{"name":"Indian Institute of Science Education and Research , , , ,","place":["Bhopal, Madhya Pradesh, India, 462066"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rohit","family":"Satardekar","sequence":"additional","affiliation":[{"name":"Indian Institute of Science Education and Research (IISER) , , , ,","place":["Tirupati, Andhra Pradesh, India, 517507"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2035-4407","authenticated-orcid":true,"given":"Raju","family":"Mukherjee","sequence":"additional","affiliation":[{"name":"Indian Institute of Science Education and Research (IISER) , , , ,","place":["Tirupati, Andhra Pradesh, India, 517507"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0238-8144","authenticated-orcid":true,"given":"Vikas","family":"Jain","sequence":"additional","affiliation":[{"name":"Krea University , , , ,","place":["Sri City, Andhra Pradesh, India, 517646"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,21]]},"reference":[{"key":"2026100610490018500_cit1","doi-asserted-by":"publisher","first-page":"563","DOI":"10.1038\/nrmicro.2016.94","article-title":"Biofilms: an emergent form of bacterial\nlife","volume":"14","author":"Flemming","year":"2016","journal-title":"Nat. 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