{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T15:59:13Z","timestamp":1791302353847,"version":"4.1.0"},"reference-count":57,"publisher":"American Chemical Society (ACS)","issue":"39","license":[{"start":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:00:00Z","timestamp":1790035200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002850","name":"Fondo Nacional de Desarrollo Cient?fico y Tecnol?gico","doi-asserted-by":"publisher","award":["1240318"],"award-info":[{"award-number":["1240318"]}],"id":[{"id":"10.13039\/501100002850","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Enzyme immobilization can be carried out in either batch or flow mode. Developing flow-through enzymatic reactions requires integrating upstream flow bioprocesses, such as flow immobilization, to minimize downtime, reduce labor requirements, and support the development of sustainable bioprocesses. In this study, Burkholderia cepacia lipase (BCL) was immobilized on Immobead 150 P, and flow esterification of 5-hydroxymethylfurfural (HMF) with oleic acid was performed to synthesize 5-hydroxymethylfurfural oleate. For batch immobilization, the optimal enzyme loading was determined to be 7 mg protein per gram of support, yielding an activity yield (IY%) of 99.9% and a protein yield (IP%) of 98.6%. In flow mode, the best immobilization results were achieved at a flow rate of 0.05 mL\/min, yielding an IY of 98% and an IP of 80% within 130 min. The resulting biocatalyst exhibited high operational stability, maintaining activity after five reaction cycles. Batch synthesis of 5-hydroxymethylfurfural oleate achieved conversions of 97% and 45% using 30 mM and 50 mM HMF, respectively, over 24 h. In flow esterification of HMF and oleic acid, conversions of 84% (30 mM HMF) and 75% (50 mM HMF) were obtained at a flow rate of 0.05 mL\/min. The space-time yield (STY) was higher in flow bioreactors, with values of 4.7 mM\/min and 5.3 mM\/min at the same flow rate for 30 mM and 50 mM HMF, respectively. This work demonstrates the integration of two flow processes\u2500lipase immobilization and HMF esterification with oleic acid\u2500highlighting the significant potential for process scale-up.<\/jats:p>","DOI":"10.1021\/acsomega.6c09089","type":"journal-article","created":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T17:01:34Z","timestamp":1790096494000},"page":"60438-60445","source":"Crossref","is-referenced-by-count":0,"title":["Integrated Flow Enzymatic Immobilization and Esterification\nfor Synthesizing 5-Hydroxymethylfurfural Oleate"],"prefix":"10.1021","volume":"11","author":[{"given":"Karina","family":"Reichel","sequence":"first","affiliation":[{"name":"Pontificia Universidad Cato\u0301lica de Chile , , Avenida Vicun\u0303a Mackenna 4860 , , ,","place":["Macul, Santiago, Chile, 7820436"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3703-133X","authenticated-orcid":true,"given":"Nadia","family":"Guajardo","sequence":"additional","affiliation":[{"name":"Pontificia Universidad Cato\u0301lica de Chile , , Avenida Vicun\u0303a Mackenna 4860 , , ,","place":["Macul, Santiago, Chile, 7820436"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Aguirre","sequence":"additional","affiliation":[{"name":"Universidad Cato\u0301lica de la Santi\u0301sima Concepcio\u0301n , , ,","place":["Concepcio\u0301n, Chile, 4090541"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,22]]},"reference":[{"issue":"1","key":"2026100610475066200_cit1","doi-asserted-by":"publisher","DOI":"10.1186\/s12934-025-02664-6","article-title":"Microbial\nLipases: Advances in Production, Purification, Biochemical Characterization,\nand Multifaceted Applications in Industry and Medicine","volume":"24","author":"Abdelaziz","year":"2025","journal-title":"Microb. 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Biol.\nMacromol."},{"key":"2026100610475066200_cit7","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2023.127358","article-title":"Microfluidic Based\nContinuous Enzyme Immobilization: A Comprehensive Review","volume":"253","author":"Patil","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"key":"2026100610475066200_cit8","doi-asserted-by":"publisher","first-page":"1735","DOI":"10.1007\/s00449-024-03066-5","article-title":"Adsorption of Extracellular\nLipase in a Packed-Bed\nReactor: An Alternative Immobilization Approach","volume":"47","author":"Freitas","year":"2024","journal-title":"Bioprocess Biosyst. 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Chem.Eng."},{"issue":"1","key":"2026100610475066200_cit45","doi-asserted-by":"publisher","DOI":"10.3390\/catal13010019","article-title":"Optimization\nand Determination of\nKinetic Parameters of the Synthesis of 5-Lauryl-Hydroxymethylfurfural\nCatalyzed by Lipases","volume":"13","author":"Uribe","year":"2023","journal-title":"Catalysts"},{"key":"2026100610475066200_cit46","doi-asserted-by":"publisher","first-page":"409","DOI":"10.1016\/j.procbio.2023.04.026","article-title":"Flow Enzymatic Esterification\nof 5-Hydroxymethylfurfural\nand Liquid\u2013Liquid Extraction of 5-Acetyl-Hydroxymethylfurfural\nUsing Deep Eutectic Solvents in Semicontinuous Mode","volume":"130","author":"Gonza\u0301lez","year":"2023","journal-title":"Process Biochem."},{"issue":"4","key":"2026100610475066200_cit47","doi-asserted-by":"publisher","first-page":"630","DOI":"10.1002\/cssc.201200931","article-title":"Lipase-Catalyzed\n(Trans)Esterification of 5-Hydroxymethylfurfural and Separation from\nHMF Esters Using Deep-Eutectic Solvents","volume":"6","author":"Krystof","year":"2013","journal-title":"ChemSusChem"},{"issue":"2","key":"2026100610475066200_cit48","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1080\/02726350590922242","article-title":"Enhanced\nVoidage Correlations for Packed Beds of Various\nParticle Shapes and Sizes","volume":"23","author":"Benyahia","year":"2005","journal-title":"Part. Sci. Technol."},{"issue":"3","key":"2026100610475066200_cit49","doi-asserted-by":"publisher","first-page":"351","DOI":"10.17113\/ftb.63.03.25.8940","article-title":"Evaluating\nPre-Immobilization and Post-Immobilization Bioimprinting Strategies\nfor the Activation of Lipases: A Case Study of LipC12","volume":"63","author":"Wielewski","year":"2025","journal-title":"Food Technol. Biotechnol."},{"key":"2026100610475066200_cit50","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1016\/j.molcatb.2015.03.002","article-title":"Immobilization\nof LipC12, a New Lipase Obtained by Metagenomics, and Its Application\nin the Synthesis of Biodiesel Esters","volume":"116","author":"Madalozzo","year":"2015","journal-title":"J. Mol.\nCatal. 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