{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T23:05:21Z","timestamp":1792191921684,"version":"4.4.0"},"reference-count":41,"publisher":"American Chemical Society (ACS)","issue":"11","license":[{"start":{"date-parts":[[2017,11,14]],"date-time":"2017-11-14T00:00:00Z","timestamp":1510617600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/pubs.acs.org\/page\/policy\/authorchoice_termsofuse.html"}],"funder":[{"DOI":"10.13039\/501100001409","name":"Department of Science and Technology, Ministry of Science and Technology","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/501100001409","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001412","name":"Council of Scientific and Industrial Research","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/501100001412","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001843","name":"Science and Engineering Research Board","doi-asserted-by":"publisher","award":["SB\/FT\/CS-198\/2013"],"award-info":[{"award-number":["SB\/FT\/CS-198\/2013"]}],"id":[{"id":"10.13039\/501100001843","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,11,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Cationic lipid-guided nucleic acid delivery holds great promise in gene therapy and genome-editing applications for treating genetic diseases. However, the major challenge lies in achieving therapeutically relevant efficiencies. Prior findings, including our own, demonstrated that asymmetry in the hydrophobic core of cationic lipids imparted superior transfection efficiencies. To this end, we have developed a lipid nanocarrier system with an asymmetric hydrophobic core (PS-Lips) derived from a mixture of fatty acids of food-grade palmstearin and compared its efficiency with symmetric palmitic acid-based nanocarrier system (P-Lip). PS-Lips exhibited superior transfection efficiencies with both plasmid DNA (pDNA) and mRNA in multiple cultured cells than the control P-Lip. More importantly, PS-Lips exhibited 2-fold superior transfections with linear nucleic acid, green fluorescent protein (GFP) mRNA in hematopoietic cells, when compared with the commercial control lipofectamine RNAiMAX. PS-Lips was also found to be effective in delivering genome-editing tools (CRISPR\/Cas9, sgRNA encoded pDNA with a reporter GFP construct) than P-Lip in HEK-293 cells. In the present study, we report that cationic liposomes derivatized from natural food-grade fat palmstearin with a natural hydrophobic core asymmetry are efficient in delivering both linear and circular nucleic acids. In particular, PS-Lips is efficient in delivering mRNA to hematopoietic cells. These findings can be further exploited in the genome-editing approach for treating \u03b2-globinopathies.<\/jats:p>","DOI":"10.1021\/acsomega.7b00935","type":"journal-article","created":{"date-parts":[[2017,11,14]],"date-time":"2017-11-14T16:13:56Z","timestamp":1510676036000},"page":"7892-7903","source":"Crossref","is-referenced-by-count":23,"title":["Green Transfection: Cationic Lipid Nanocarrier System Derivatized from\nVegetable Fat, Palmstearin Enhances Nucleic Acid Transfections"],"prefix":"10.1021","volume":"2","author":[{"given":"Priya","family":"Dharmalingam","sequence":"first","affiliation":[{"name":"Christian Medical College Campus , (a Unit of inStem, Bengaluru), , Bagayam, ,","place":["Vellore, India, 632002"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hari Krishna R.","family":"Rachamalla","sequence":"additional","affiliation":[{"name":"CSIR-Indian Institute of Chemical Technology , , ,","place":["Hyderabad, India, 500 007"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brijesh","family":"Lohchania","sequence":"additional","affiliation":[{"name":"Christian Medical College Campus , (a Unit of inStem, Bengaluru), , Bagayam, ,","place":["Vellore, India, 632002"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bhanuprasad","family":"Bandlamudi","sequence":"additional","affiliation":[{"name":"Christian Medical College Campus , (a Unit of inStem, Bengaluru), , Bagayam, ,","place":["Vellore, India, 632002"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saravanabhavan","family":"Thangavel","sequence":"additional","affiliation":[{"name":"Christian Medical College Campus , (a Unit of inStem, Bengaluru), , Bagayam, ,","place":["Vellore, India, 632002"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohankumar K.","family":"Murugesan","sequence":"additional","affiliation":[{"name":"Christian Medical College Campus , (a Unit of inStem, Bengaluru), , Bagayam, ,","place":["Vellore, India, 632002"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajkumar","family":"Banerjee","sequence":"additional","affiliation":[{"name":"CSIR-Indian Institute of Chemical Technology , , ,","place":["Hyderabad, India, 500 007"]},{"name":"CSIR-Indian Institute of Chemical Technology , , Tarnaka, ,","place":["Hyderabad, India, 500007"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0734-8320","authenticated-orcid":true,"given":"Arabinda","family":"Chaudhuri","sequence":"additional","affiliation":[{"name":"CSIR-Indian Institute of Chemical Technology , , ,","place":["Hyderabad, India, 500 007"]},{"name":"CSIR-Indian Institute of Chemical Technology , , Tarnaka, ,","place":["Hyderabad, India, 500007"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chandrashekhar","family":"Voshavar","sequence":"additional","affiliation":[{"name":"CSIR-Indian Institute of Chemical Technology , , ,","place":["Hyderabad, India, 500 007"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2451-3359","authenticated-orcid":true,"given":"Srujan","family":"Marepally","sequence":"additional","affiliation":[{"name":"Christian Medical College Campus , (a Unit of inStem, Bengaluru), , Bagayam, ,","place":["Vellore, India, 632002"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2017,11,14]]},"reference":[{"key":"2026090201493744300_cit1","doi-asserted-by":"publisher","first-page":"351","DOI":"10.1038\/nature15818","article-title":"Gene therapy\nreturns to centre stage","volume":"526","author":"Naldini","year":"2015","journal-title":"Nature"},{"key":"2026090201493744300_cit2","doi-asserted-by":"publisher","first-page":"897","DOI":"10.1089\/scd.2015.0324","article-title":"Preclinical\nBiosafety Evaluation of Genetically Modified Human Adipose Tissue-Derived\nMesenchymal Stem Cells for Clinical Applications to Brainstem Glioma","volume":"25","author":"Choi","year":"2016","journal-title":"Stem Cells Dev."},{"key":"2026090201493744300_cit3","doi-asserted-by":"publisher","first-page":"1435","DOI":"10.1016\/j.jcyt.2016.09.003","article-title":"Genetically\nmodified mesenchymal\nstromal cells in cancer therapy","volume":"18","author":"Sage","year":"2016","journal-title":"Cytotherapy"},{"key":"2026090201493744300_cit4","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1242\/dmm.018036","article-title":"Genetically modified\nT cells in cancer therapy: opportunities and\nchallenges","volume":"8","author":"Sharpe","year":"2015","journal-title":"Dis. 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