{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:56:56Z","timestamp":1791356216152,"version":"4.3.1"},"reference-count":24,"publisher":"American Chemical Society (ACS)","issue":"9","license":[{"start":{"date-parts":[[2017,9,19]],"date-time":"2017-09-19T00:00:00Z","timestamp":1505779200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/pubs.acs.org\/page\/policy\/authorchoice_termsofuse.html"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,9,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Early diagnosis of dopamine and serotonin metabolic defects is of importance notably because of the availability of therapeutic strategies able to prevent the associated progressive brain dysfunction. The diagnosis of these diseases relies on the determination of monoamine metabolites and pterins in cerebrospinal fluid (CSF). Current methods involve at least two high-performance liquid chromatography runs of CSF analysis. The first one is devoted to the quantification of dopamine and serotonin metabolites and the second one to the quantification of pterins. Here, we describe a single-step method to measure monoamine neurotransmitter metabolites and pterins of interest in less than 10 min by ultrahigh-performance liquid chromatography coupled to sequential coulometric oxidation and fluorescence detections. All target compounds were quantified in CSF with a small volume (50 \u03bcL) and a single filtration step for sample preparation and analysis. After validation, the proposed method was applied to the determination of age-related reference ranges in the CSF of target compounds from a series of 1372 samples collected in France from 2008 to 2014. In the same period, the results obtained for 19 CSF samples from patients with known neurotransmitter disorders and 115 CSF samples with known immune system activation confirmed the expected pattern of changes in monoamine metabolites and pterins.<\/jats:p>","DOI":"10.1021\/acsomega.7b01008","type":"journal-article","created":{"date-parts":[[2017,9,19]],"date-time":"2017-09-19T10:39:43Z","timestamp":1505817583000},"page":"5962-5972","source":"Crossref","is-referenced-by-count":16,"title":["Single-Step Rapid Diagnosis of Dopamine and Serotonin\nMetabolism Disorders"],"prefix":"10.1021","volume":"2","author":[{"given":"Aure\u0301lien","family":"Lo","sequence":"first","affiliation":[{"name":"Universite\u0301 Paris Sud , , Plateau de Moulon , ,","place":["Orsay, France, 91400"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Guibal","sequence":"additional","affiliation":[{"name":"Universite\u0301 Paris Sud , , Plateau de Moulon , ,","place":["Orsay, France, 91400"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diane","family":"Doummar","sequence":"additional","affiliation":[{"name":"Services de Neurope\u0301diatrie et de Biochimie, Groupe Hospitalier Trousseau\u2500Laroche\u2500Guyon , 26 avenue du Dr Arnold Netter , ,","place":["Paris, France, 75012"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Rodriguez","sequence":"additional","affiliation":[{"name":"Services de Neurope\u0301diatrie et de Biochimie, Groupe Hospitalier Trousseau\u2500Laroche\u2500Guyon , 26 avenue du Dr Arnold Netter , ,","place":["Paris, France, 75012"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Yves","family":"Hautem","sequence":"additional","affiliation":[{"name":"Services de Neurope\u0301diatrie et de Biochimie, Groupe Hospitalier Trousseau\u2500Laroche\u2500Guyon , 26 avenue du Dr Arnold Netter , ,","place":["Paris, France, 75012"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Re\u0301my","family":"Couderc","sequence":"additional","affiliation":[{"name":"Services de Neurope\u0301diatrie et de Biochimie, Groupe Hospitalier Trousseau\u2500Laroche\u2500Guyon , 26 avenue du Dr Arnold Netter , ,","place":["Paris, France, 75012"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"Billette De Villemeur","sequence":"additional","affiliation":[{"name":"Services de Neurope\u0301diatrie et de Biochimie, Groupe Hospitalier Trousseau\u2500Laroche\u2500Guyon , 26 avenue du Dr Arnold Netter , ,","place":["Paris, France, 75012"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuel","family":"Roze","sequence":"additional","affiliation":[{"name":"Pitie\u0301-Salpe\u0302trie\u0300re Hospital, AP-HP , , ,","place":["Paris, France, 75013"]},{"name":"UMR S 952, INSERM, Paris 6 University , ,","place":["Paris, France, 75005"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Chaminade","sequence":"additional","affiliation":[{"name":"Universite\u0301 Paris Sud , , Plateau de Moulon , ,","place":["Orsay, France, 91400"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fathi","family":"Moussa","sequence":"additional","affiliation":[{"name":"Universite\u0301 Paris Sud , , Plateau de Moulon , ,","place":["Orsay, France, 91400"]},{"name":"Services de Neurope\u0301diatrie et de Biochimie, Groupe Hospitalier Trousseau\u2500Laroche\u2500Guyon , 26 avenue du Dr Arnold Netter , ,","place":["Paris, France, 75012"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2017,9,19]]},"reference":[{"key":"2026090317513996400_cit1","doi-asserted-by":"crossref","first-page":"1568S","DOI":"10.1093\/jn\/137.6.1568S","article-title":"Inherited\ndisorders affecting dopamine and serotonin: critical neurotransmitters\nderived from aromatic amino acids","volume":"137","author":"Hyland","year":"2007","journal-title":"J. Nutr."},{"key":"2026090317513996400_cit2","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1007\/s10545-009-1067-2","article-title":"Disorders\nof biopterin metabolism","volume":"32","author":"Longo","year":"2009","journal-title":"J. Inherited Metab.\nDis."},{"key":"2026090317513996400_cit3","doi-asserted-by":"publisher","first-page":"721","DOI":"10.1016\/s1474-4422(11)70141-7","article-title":"The monoamine\nneurotransmitter disorders: an expanding range of neurological syndromes","volume":"10","author":"Kurian","year":"2011","journal-title":"Lancet Neurol."},{"key":"2026090317513996400_cit4","doi-asserted-by":"publisher","first-page":"415","DOI":"10.1016\/s0301-0082(99)00059-3","article-title":"The role of 6R-tetrahydrobiopterin\nin the nervous system","volume":"61","author":"Koshimura","year":"2000","journal-title":"Prog. Neurobiol."},{"key":"2026090317513996400_cit5","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1007\/978-3-642-55878-8_7","article-title":"Disorders of\nphenylalanine and tetrahydrobiopterin metabolism","volume-title":"Physician\u2019s Guide to the Laboratory Diagnosis of\nMetabolic Diseases","author":"Blau","year":"2003","edition":"2"},{"key":"2026090317513996400_cit6","first-page":"203","article-title":"Biogenic monoamine disorders","volume-title":"Inherited Metabolic Disease in Adults: A Clinical Guide","author":"Roze","year":"2016","edition":"1"},{"key":"2026090317513996400_cit7","doi-asserted-by":"publisher","first-page":"633","DOI":"10.1373\/clinchem.2007.099986","article-title":"Clinical utility\nof monoamine neurotransmitter metabolite analysis in cerebrospinal\nfluid","volume":"54","author":"Hyland","year":"2008","journal-title":"Clin. Chem."},{"key":"2026090317513996400_cit8","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1016\/j.ymgme.2009.01.010","article-title":"A new perspective\non the treatment\nof aromatic L-amino acid decarboxylase deficiency","volume":"97","author":"Allen","year":"2009","journal-title":"Mol. Genet. Metab."},{"key":"2026090317513996400_cit9","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1093\/sleep\/33.3.307","article-title":"Sleep and Rhythm Consequences\nof a Genetically Induced Loss of Serotonin","volume":"33","author":"Leu-Semenescu","year":"2010","journal-title":"Sleep"},{"key":"2026090317513996400_cit10","doi-asserted-by":"publisher","first-page":"176","DOI":"10.1016\/0003-2697(80)90336-x","article-title":"Analysis of reduced\nforms of biopterin in biological\ntissues and fluids","volume":"102","author":"Fukushima","year":"1980","journal-title":"Anal. Biochem."},{"key":"2026090317513996400_cit11","doi-asserted-by":"publisher","first-page":"285","DOI":"10.1016\/s0378-4347(00)83594-x","article-title":"Estimation\nof tetrahydrobiopterin\nand other pterins in cerebrospinal fluid using reversed-phase high-performance\nliquid chromatography with electrochemical and fluorescence detection","volume":"381","author":"Howells","year":"1986","journal-title":"J. Chromatogr. B: Biomed. Sci. Appl."},{"key":"2026090317513996400_cit12","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1093\/clinchem\/34.4.680","article-title":"Simultaneous determination\nof four\nmonoamine metabolites and serotonin in cerebrospinal fluid by \u201cHigh-Performance\u201d\nLiquid Chromatography with electrochemical detection; application\nfor patients with Alzheimer\u2019s Disease","volume":"34","author":"Koyama","year":"1988","journal-title":"Clin. 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Chromatogr."},{"key":"2026090317513996400_cit15","doi-asserted-by":"publisher","first-page":"423","DOI":"10.1016\/s0378-4347(00)00285-1","article-title":"Liquid chromatography\u2013electrospray\ntandem mass spectrometry of acidic monoamine metabolites","volume":"744","author":"Manini","year":"2000","journal-title":"J. Chromatogr. B: Biomed. Sci. Appl."},{"key":"2026090317513996400_cit16","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1016\/j.jneumeth.2004.08.007","article-title":"HPLC with electrochemical and fluorescence detection\nprocedures for the diagnosis of inborn errors of biogenic amines and\npterins","volume":"142","author":"Ormazabal","year":"2005","journal-title":"J. Neurosci. Methods"},{"key":"2026090317513996400_cit17","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1016\/j.ymgme.2008.07.004","article-title":"Technical\nand biochemical factors\naffecting cerebrospinal fluid 5-MTHF, biopterin and neopterin concentrations","volume":"95","author":"Verbeek","year":"2008","journal-title":"Mol. Genet. Metab."},{"key":"2026090317513996400_cit18","doi-asserted-by":"publisher","first-page":"533","DOI":"10.1021\/cn4001928","article-title":"Simultaneous Determination\nof All Forms of Biopterin\nand Neopterin in Cerebrospinal Fluid","volume":"5","author":"Guibal","year":"2014","journal-title":"ACS Chem.\nNeurosci."},{"key":"2026090317513996400_cit19","doi-asserted-by":"crossref","unstructured":"Arning, E.; Bottiglieri, T.\n          LC-MS\/MS analysis\nof cerebrospinal fluid metabolites in the pterin biosynthetic pathway.  JIMD Reports; Springer, 2014; Vol. 29, pp 1\u20139.","DOI":"10.1007\/8904_2014_336"},{"key":"2026090317513996400_cit20","doi-asserted-by":"publisher","first-page":"1405","DOI":"10.1002\/rcm.2459","article-title":"Detection\nof 28 neurotransmitters and related compounds\nin biological fluids by liquid chromatography\/tandem mass spectrometry","volume":"20","author":"Bourcier","year":"2006","journal-title":"Rapid Commun. Mass Spectrom."},{"key":"2026090317513996400_cit21","first-page":"21","article-title":"Power comparisons of Shapiro-Wilk, Kolmogorov-Smirnov,\nLilliefors and Anderson-Darling tests","volume":"2","author":"Razali","year":"2011","journal-title":"J. Stat.\nModel. Anal."},{"key":"2026090317513996400_cit22","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1203\/00006450-199307000-00003","article-title":"Cerebrospinal fluid\nconcentrations of pterins and metabolites of\nserotonin and dopamine in a pediatric reference population","volume":"34","author":"Hyland","year":"1993","journal-title":"Pediatr. Res."},{"key":"2026090317513996400_cit23","doi-asserted-by":"publisher","first-page":"559","DOI":"10.1111\/dmcn.12116","article-title":"Homovanillic acid\nin cerebrospinal fluid of 1388 children\nwith neurological disorders","volume":"55","author":"Molero-Luis","year":"2013","journal-title":"Dev. Med. Child.\nNeurol."},{"key":"2026090317513996400_cit24","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1186\/1742-6405-7-15","article-title":"Cerebrospinal fluid\nneopterin: an informative biomarker of central nervous system immune\nactivation in HIV-1 infection","volume":"7","author":"Hagberg","year":"2010","journal-title":"AIDS Res. Ther."}],"container-title":["ACS Omega"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsomega.7b01008","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/acsodf\/article-pdf\/2\/9\/5962\/68310427\/acsomega.7b01008.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/acsodf\/article-pdf\/2\/9\/5962\/68310427\/acsomega.7b01008.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T21:51:49Z","timestamp":1788472309000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/acsodf\/article\/2\/9\/5962\/751244\/Single-Step-Rapid-Diagnosis-of-Dopamine-and"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,9,19]]},"references-count":24,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2017,9,19]]},"published-print":{"date-parts":[[2017,9,30]]}},"URL":"https:\/\/doi.org\/10.1021\/acsomega.7b01008","relation":{},"ISSN":["2470-1343"],"issn-type":[{"value":"2470-1343","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,9,19]]}}}