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Introduction of dicationic or dianionic groups imparts water solubility and prevents translocation of the dye through the plasma membrane for highly effective labeling. The dicationic form is particularly well localized to the plasma membrane and resists quenching even after &amp;gt;8 min of continuous light exposure. The dyes are almost completely nonemissive in water and other highly polar solvents, but display high-fluorescence yields in chloroform and upon insertion into the extracellular leaflet.<\/jats:p>","DOI":"10.1021\/acsomega.8b01487","type":"journal-article","created":{"date-parts":[[2018,10,15]],"date-time":"2018-10-15T16:03:48Z","timestamp":1539619428000},"page":"13195-13199","source":"Crossref","is-referenced-by-count":14,"title":["Synthesis of Water-Soluble Far-Red-Emitting Amphiphilic\nBODIPY Dyes"],"prefix":"10.1021","volume":"3","author":[{"given":"Kevin M.","family":"Bardon","sequence":"first","affiliation":[{"name":"Akita Innovations LLC , 267 Boston Road, Suite 11 , , ,","place":["North Billerica, Massachusetts, United States, 01862"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott","family":"Selfridge","sequence":"additional","affiliation":[{"name":"Akita Innovations LLC , 267 Boston Road, Suite 11 , , ,","place":["North Billerica, Massachusetts, United States, 01862"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dany S.","family":"Adams","sequence":"additional","affiliation":[{"name":"Tufts University , , 200 Boston Avenue , , ,","place":["Medford, Massachusetts, United States, 02155"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard A.","family":"Minns","sequence":"additional","affiliation":[{"name":"Akita Innovations LLC , 267 Boston Road, Suite 11 , , ,","place":["North Billerica, Massachusetts, United States, 01862"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9296-3928","authenticated-orcid":true,"given":"Robert","family":"Pawle","sequence":"additional","affiliation":[{"name":"Akita Innovations LLC , 267 Boston Road, Suite 11 , , ,","place":["North Billerica, Massachusetts, United States, 01862"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy C.","family":"Adams","sequence":"additional","affiliation":[{"name":"Akita Innovations LLC , 267 Boston Road, Suite 11 , , ,","place":["North Billerica, Massachusetts, United States, 01862"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larry","family":"Takiff","sequence":"additional","affiliation":[{"name":"Akita Innovations LLC , 267 Boston Road, Suite 11 , , ,","place":["North Billerica, Massachusetts, United States, 01862"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2018,10,15]]},"reference":[{"key":"2026090210343193100_cit1","doi-asserted-by":"publisher","first-page":"580","DOI":"10.1038\/nature06917","article-title":"Imaging in the era\nof molecular oncology","volume":"452","author":"Weissleder","year":"2008","journal-title":"Nature"},{"key":"2026090210343193100_cit2","volume-title":"The\nMolecular Probes Handbook: A Guide to Fluorescent Probes and Labelling\nTechnologies","year":"2010","edition":"11"},{"key":"2026090210343193100_cit3","doi-asserted-by":"publisher","first-page":"1347","DOI":"10.2147\/IJN.S60206","article-title":"Near-Infrared\nFluorescent Prodes in Cancer Imaging and Therapy: an Emerging Field","volume":"9","author":"Yi","year":"2014","journal-title":"Int. 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