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The synthesized compounds were tested for in vitro antibacterial activity against three Gram negative (Escherichia coli, Pseudomonas putida, and Salmonella typhi) and two Gram positive (Bacillus subtilis, and Staphylococcus aureus) bacteria. Among all tested compounds, 8ga, 8gc, and 8gd exhibited promising minimum inhibitory concentration (MIC) values (4\u20138 \u03bcg\/mL) for all bacterial strains tested as compared to the positive control ciprofloxacin. The synthesized compounds were also evaluated for their in vitro antifungal activity against Aspergillus niger and Candida albicans and compounds 8ga, 8gc, and 8gd having potential antibacterial activity also showed pronounced antifungal activity (MIC values 8\u201316 \u03bcg\/mL) against both strains. The bactericidal assay by propidium iodide and live\u2013dead bacterial cell screening using a mixture of acridine orange\/ethidium bromide (AO\/Et\u00b7Br) showed considerable changes in the bacterial cell membrane, which might be the cause or consequence of cell death. Moreover, the hemolytic activity for most potent compounds (8ga, 8gc, and 8gd) showed their safety profile toward human blood cells.<\/jats:p>","DOI":"10.1021\/acsomega.8b01592","type":"journal-article","created":{"date-parts":[[2018,11,30]],"date-time":"2018-11-30T10:05:27Z","timestamp":1543572327000},"page":"16338-16346","source":"Crossref","is-referenced-by-count":68,"title":["Design and Synthesis of Imidazo\/Benzimidazo[1,2-\n                    <i>c<\/i>\n                    ]quinazoline Derivatives and Evaluation of Their Antimicrobial\nActivity"],"prefix":"10.1021","volume":"3","author":[{"given":"Nitesh Kumar","family":"Nandwana","sequence":"first","affiliation":[{"name":"Birla Institute of Technology and Science , , , ,","place":["Pilani, Rajasthan, India, 333031"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajnish Prakash","family":"Singh","sequence":"additional","affiliation":[{"name":"Birla Institute of Technology and Science , , , ,","place":["Pilani, Rajasthan, India, 333031"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Om P. S.","family":"Patel","sequence":"additional","affiliation":[{"name":"Birla Institute of Technology and Science , , , ,","place":["Pilani, Rajasthan, India, 333031"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiv","family":"Dhiman","sequence":"additional","affiliation":[{"name":"Birla Institute of Technology and Science , , , ,","place":["Pilani, Rajasthan, India, 333031"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hitesh Kumar","family":"Saini","sequence":"additional","affiliation":[{"name":"Birla Institute of Technology and Science , , , ,","place":["Pilani, Rajasthan, India, 333031"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prabhat N.","family":"Jha","sequence":"additional","affiliation":[{"name":"Birla Institute of Technology and Science , , , ,","place":["Pilani, Rajasthan, India, 333031"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4699-3178","authenticated-orcid":true,"given":"Anil","family":"Kumar","sequence":"additional","affiliation":[{"name":"Birla Institute of Technology and Science , , , ,","place":["Pilani, Rajasthan, India, 333031"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2018,11,30]]},"reference":[{"key":"2026090206303160000_cit1","doi-asserted-by":"publisher","first-page":"520","DOI":"10.1056\/nejm199808203390806","article-title":"Staphylococcus\naureus infections","volume":"339","author":"Lowy","year":"1998","journal-title":"N. 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