{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T18:54:22Z","timestamp":1790016862138,"version":"4.0.1"},"reference-count":37,"publisher":"American Chemical Society (ACS)","issue":"4","license":[{"start":{"date-parts":[[2019,4,15]],"date-time":"2019-04-15T00:00:00Z","timestamp":1555286400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/pubs.acs.org\/page\/policy\/authorchoice_termsofuse.html"}],"funder":[{"DOI":"10.13039\/100000165","name":"Division of Chemistry","doi-asserted-by":"publisher","award":["CHE-1506740"],"award-info":[{"award-number":["CHE-1506740"]}],"id":[{"id":"10.13039\/100000165","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006827","name":"Florida Department of Health","doi-asserted-by":"publisher","award":["7ZK01"],"award-info":[{"award-number":["7ZK01"]}],"id":[{"id":"10.13039\/100006827","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008530","name":"Leonard M. Miller School of Medicine","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R01GM047915"],"award-info":[{"award-number":["R01GM047915"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R01GM127706"],"award-info":[{"award-number":["R01GM127706"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,4,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Zika virus (ZIKV) is an arbovirus that caused widespread panic beginning in 2015 in northeastern Brazil due to the threatening link between infection and fetal abnormalities such as microcephaly, spontaneous abortions, and stillbirths. Since the epidemic began, the virus has been further investigated, unveiling that the long-term dangers of ZIKV infection go beyond fetal neurological impairment. Characterization of the active infection has proven difficult as only 20% of infected individuals are symptomatic. Additionally, ZIKV is often misdiagnosed due to serological cross-reactivity with similar flaviviruses such as dengue, yellow fever, and West Nile. To date, there is no approved vaccine or therapy against ZIKV, highlighting the urgent need to accurately identify active infection to help minimize the spread of the virus. Herein, we describe a highly specific and sensitive enzyme-linked immunosorbent assay to detect early active ZIKV using neutralizing human monoclonal antibodies isolated from infected patients in Brazil that do not cross-react with dengue viruses 1\u20134 and bind directly to a ZIKV immunodominant epitope. The calculated limits of detection of active ZIKV fall within the physiological ranges of the virus in human bodily fluids. This selective immunoassay creates the platform required for future translation toward a point-of-care assay for ZIKV, a necessity to diagnose active ZIKV in the remote regions of which it thrives.<\/jats:p>","DOI":"10.1021\/acsomega.9b00374","type":"journal-article","created":{"date-parts":[[2019,4,15]],"date-time":"2019-04-15T10:03:53Z","timestamp":1555322633000},"page":"6808-6818","source":"Crossref","is-referenced-by-count":14,"title":["Highly Sensitive and Selective Direct Detection of\nZika Virus Particles in Human\nBodily Fluids for Accurate Early Diagnosis of Infection"],"prefix":"10.1021","volume":"4","author":[{"given":"Devon C.","family":"Pawley","sequence":"first","affiliation":[{"name":"University of Miami , , , ,","place":["Miami, Florida, United States, 33136"]},{"name":"Dr. JT Macdonald Foundation Biomedical Nanotechnology Institute of the University of Miami , , ,","place":["Miami, Florida, United States, 33136"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Ricciardi","sequence":"additional","affiliation":[{"name":"University of Miami , , , ,","place":["Miami, Florida, United States, 33136"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emre","family":"Dikici","sequence":"additional","affiliation":[{"name":"University of Miami , , , ,","place":["Miami, Florida, United States, 33136"]},{"name":"Dr. JT Macdonald Foundation Biomedical Nanotechnology Institute of the University of Miami , , ,","place":["Miami, Florida, United States, 33136"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4926-5355","authenticated-orcid":true,"given":"Sapna K.","family":"Deo","sequence":"additional","affiliation":[{"name":"University of Miami , , , ,","place":["Miami, Florida, United States, 33136"]},{"name":"Dr. JT Macdonald Foundation Biomedical Nanotechnology Institute of the University of Miami , , ,","place":["Miami, Florida, United States, 33136"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4760-5528","authenticated-orcid":true,"given":"Sylvia","family":"Daunert","sequence":"additional","affiliation":[{"name":"University of Miami , , , ,","place":["Miami, Florida, United States, 33136"]},{"name":"Dr. JT Macdonald Foundation Biomedical Nanotechnology Institute of the University of Miami , , ,","place":["Miami, Florida, United States, 33136"]},{"name":"University of Miami Clinical and Translational Science Institute , , ,","place":["Miami, Florida, United States, 33136"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2019,4,15]]},"reference":[{"key":"2026090407163723000_cit1","doi-asserted-by":"publisher","first-page":"509","DOI":"10.1016\/0035-9203(52)90042-4","article-title":"Zika virus.\nI. Isolations and serological\nspecificity","volume":"46","author":"Dick","year":"1952","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"2026090407163723000_cit2","doi-asserted-by":"publisher","first-page":"350","DOI":"10.1097\/ANC.0000000000000557","article-title":"Zika Virus\nInfection: A Vector-Borne Threat to Pregnant\nWomen and Infants","volume":"18","author":"Grazel","year":"2018","journal-title":"Adv. Neonatal Care"},{"key":"2026090407163723000_cit3","doi-asserted-by":"publisher","first-page":"1990","DOI":"10.3390\/ijerph15091990","article-title":"Growth\nand Development of Children\nwith Microcephaly Associated with Congenital Zika Virus Syndrome in\nBrazil","volume":"15","author":"Franc\u0327a","year":"2018","journal-title":"Int. J. Environ. Res. Public Health"},{"key":"2026090407163723000_cit4","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1016\/j.ajog.2018.08.035","article-title":"Zika Virus and the Non-Microcephalic\nFetus: Why We Should Still Worry","author":"Walker","year":"2018","journal-title":"Am. J. Obstet.\nGynecol."},{"key":"2026090407163723000_cit5","doi-asserted-by":"publisher","DOI":"10.1136\/bmjgh-2017-000530","article-title":"Zika, chikungunya\nand dengue: the\ncauses and threats of new and re-emerging arboviral diseases","volume":"3","author":"Paixa\u0303o","year":"2018","journal-title":"BMJ Global Health"},{"key":"2026090407163723000_cit6","doi-asserted-by":"publisher","first-page":"7289","DOI":"10.1021\/acs.analchem.6b01632","article-title":"Instrument-Free Point-of-Care\nMolecular Detection of Zika Virus","volume":"88","author":"Song","year":"2016","journal-title":"Anal. Chem."},{"key":"2026090407163723000_cit7","doi-asserted-by":"publisher","first-page":"420","DOI":"10.1016\/j.bios.2016.06.085","article-title":"Attomolar Zika virus oligonucleotide\ndetection based on loop-mediated isothermal amplification and AC susceptometry","volume":"86","author":"Tian","year":"2016","journal-title":"Biosens. Bioelectron."},{"key":"2026090407163723000_cit8","doi-asserted-by":"publisher","first-page":"1577","DOI":"10.3201\/eid2309.162043","article-title":"Evaluation of 5 Commercially\nAvailable Zika Virus Immunoassays","volume":"23","author":"Safronetz","year":"2017","journal-title":"Emerging\nInfect. Dis."},{"key":"2026090407163723000_cit9","doi-asserted-by":"publisher","first-page":"1081","DOI":"10.1016\/j.cell.2016.05.008","article-title":"Zika Virus Infection\nduring Pregnancy in Mice Causes Placental Damage and Fetal Demise","volume":"165","author":"Miner","year":"2016","journal-title":"Cell"},{"key":"2026090407163723000_cit10","doi-asserted-by":"publisher","DOI":"10.1038\/srep32227","article-title":"An ultrasensitive electrogenerated\nchemiluminescence-based immunoassay for specific detection of Zika\nvirus","volume":"6","author":"Acharya","year":"2016","journal-title":"Sci. Rep."},{"key":"2026090407163723000_cit11","doi-asserted-by":"publisher","first-page":"29","DOI":"10.3390\/diagnostics7020029","article-title":"Analytical Tools to Improve Optimization\nProcedures for Lateral Flow Assays","volume":"7","author":"Hsieh","year":"2017","journal-title":"Diagnostics"},{"key":"2026090407163723000_cit12","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0005655","article-title":"A human\ninferred germline antibody binds to an immunodominant epitope and\nneutralizes Zika virus","volume":"11","author":"Magnani","year":"2017","journal-title":"PLoS Neglected Trop.\nDis."},{"key":"2026090407163723000_cit13","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1007\/978-1-59745-240-3","volume-title":"Lateral Flow Immunoassay","author":"Wong","year":"2009"},{"key":"2026090407163723000_cit14","doi-asserted-by":"publisher","first-page":"879.e7","DOI":"10.1016\/j.amjmed.2016.02.027","article-title":"Re-Emergence of\nZika Virus: A Review on Pathogenesis, Clinical Manifestations, Diagnosis,\nTreatment, and Prevention","volume":"129","author":"Shuaib","year":"2016","journal-title":"Am. J. Med."},{"key":"2026090407163723000_cit15","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2016.21.10.30159","article-title":"Isolation\nof infectious Zika virus from saliva and prolonged viral RNA shedding\nin a traveller returning from the Dominican Republic to Italy","volume":"21","author":"Barzon","year":"2016","journal-title":"Eurosurveillance"},{"key":"2026090407163723000_cit16","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES2014.19.13.20751","article-title":"Evidence of perinatal transmission\nof Zika virus, French Polynesia,\nDecember 2013 and February 2014","volume":"19","author":"Besnard","year":"2014","journal-title":"Eurosurveillance"},{"key":"2026090407163723000_cit17","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1016\/j.jcv.2016.02.009","article-title":"Prolonged detection of Zika virus\nRNA in urine samples during the ongoing Zika virus epidemic in Brazil","volume":"77","author":"Campos","year":"2016","journal-title":"J. Clin. Virol."},{"key":"2026090407163723000_cit18","doi-asserted-by":"publisher","first-page":"1035","DOI":"10.4269\/ajtmh.14-0151","article-title":"First case of Zika virus infection in a returning Canadian\ntraveler","volume":"91","author":"Fonseca","year":"2014","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"2026090407163723000_cit19","doi-asserted-by":"publisher","first-page":"84","DOI":"10.3201\/eid2101.140894","article-title":"Detection\nof Zika virus in urine","volume":"21","author":"Gourinat","year":"2015","journal-title":"Emerging Infect. Dis."},{"key":"2026090407163723000_cit20","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES2014.19.4.20683","article-title":"Two cases\nof Zika fever imported from French Polynesia to Japan, December 2013\nto January 2014 [corrected]","volume":"19","author":"Kutsuna","year":"2014","journal-title":"Eurosurveillance"},{"key":"2026090407163723000_cit21","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES2014.19.14.20761","article-title":"Potential for Zika virus transmission\nthrough blood\ntransfusion demonstrated during an outbreak in French Polynesia, November\n2013 to February 2014","volume":"19","author":"Musso","year":"2014","journal-title":"Eurosurveillance"},{"key":"2026090407163723000_cit22","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1016\/j.jcv.2015.04.021","article-title":"Detection\nof Zika virus in saliva","volume":"68","author":"Musso","year":"2015","journal-title":"J. Clin. Virol."},{"key":"2026090407163723000_cit23","doi-asserted-by":"publisher","first-page":"1961","DOI":"10.1007\/s11255-016-1406-9","article-title":"Advantage of urine based molecular\ndiagnosis of Zika virus","volume":"48","author":"Lamb","year":"2016","journal-title":"Int. Urol. Nephrol."},{"key":"2026090407163723000_cit24","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2016.21.6.30131","article-title":"Zika virus\ninfections in three travellers returning from South America and the\nCaribbean respectively, to Montpellier, France, December 2015 to January\n2016","volume":"21","author":"Maria","year":"2016","journal-title":"Eurosurveillance"},{"key":"2026090407163723000_cit25","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2016.21.9.30154","article-title":"Zika virus\ndetection in urine from patients with Guillain-Barre syndrome on Martinique,\nJanuary 2016","volume":"21","author":"Roze","year":"2016","journal-title":"Eurosurveillance"},{"key":"2026090407163723000_cit26","doi-asserted-by":"publisher","DOI":"10.1093\/jtm\/tav011","article-title":"Zika fever\nimported from Thailand to Japan, and diagnosed\nby PCR in the urines","volume":"23","author":"Shinohara","year":"2016","journal-title":"J Travel Med."},{"key":"2026090407163723000_cit27","doi-asserted-by":"publisher","first-page":"940","DOI":"10.3201\/eid2205.160107","article-title":"Detection\nof Zika Virus in Semen","volume":"22","author":"Atkinson","year":"2016","journal-title":"Emerging Infect. Dis."},{"key":"2026090407163723000_cit28","doi-asserted-by":"publisher","first-page":"405","DOI":"10.1016\/S1473-3099(16)00138-9","article-title":"Zika virus:\nhigh infectious viral load in semen, a new sexually transmitted pathogen?","volume":"16","author":"Mansuy","year":"2016","journal-title":"Lancet Infect. Dis."},{"key":"2026090407163723000_cit29","doi-asserted-by":"publisher","first-page":"359","DOI":"10.3201\/eid2102.141363","article-title":"Potential\nsexual transmission of Zika virus","volume":"21","author":"Musso","year":"2015","journal-title":"Emerging\nInfect. Dis."},{"key":"2026090407163723000_cit30","doi-asserted-by":"publisher","first-page":"435","DOI":"10.1007\/s10815-016-0684-6","article-title":"Zika virus infection\nin semen: a call to action and\nresearch","volume":"33","author":"Rowland","year":"2016","journal-title":"J. Assist. Reprod. Genet."},{"key":"2026090407163723000_cit31","doi-asserted-by":"publisher","first-page":"1051","DOI":"10.1016\/S0140-6736(16)00624-3","article-title":"Infectious\nZika viral particles in breastmilk","volume":"387","author":"Dupont-Rouzeyrol","year":"2016","journal-title":"Lancet"},{"key":"2026090407163723000_cit32","doi-asserted-by":"publisher","first-page":"773","DOI":"10.3201\/eid2305.161630","article-title":"Lack of\nDurable\nCross-Neutralizing Antibodies Against Zika Virus from Dengue Virus\nInfection","volume":"23","author":"Collins","year":"2017","journal-title":"Emerging Infect. Dis."},{"key":"2026090407163723000_cit33","doi-asserted-by":"publisher","first-page":"9557","DOI":"10.1128\/JVI.00080-06","article-title":"Cryptic properties\nof a cluster of dominant flavivirus cross-reactive antigenic sites","volume":"80","author":"Stiasny","year":"2006","journal-title":"J. Virol."},{"key":"2026090407163723000_cit34","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2019.02.008","article-title":"Pregnancy outcomes after maternal Zika virus infection\nin a non-endemic region: prospective cohort study","author":"Rodo\u0301","year":"2019","journal-title":"Clin. Microbiol. Infect."},{"key":"2026090407163723000_cit35","doi-asserted-by":"publisher","first-page":"179","DOI":"10.3389\/fneur.2015.00179","article-title":"A Practical Guide to Immunoassay\nMethod Validation","volume":"6","author":"Andreasson","year":"2015","journal-title":"Front. Neurol."},{"key":"2026090407163723000_cit36","doi-asserted-by":"publisher","first-page":"112","DOI":"10.1016\/j.jim.2007.09.017","article-title":"Efficient generation of monoclonal\nantibodies from\nsingle human B cells by single cell RT-PCR and expression vector cloning","volume":"329","author":"Tiller","year":"2008","journal-title":"J. Immunol. Methods"},{"key":"2026090407163723000_cit37","doi-asserted-by":"publisher","first-page":"W34","DOI":"10.1093\/nar\/gkt382","article-title":"IgBLAST: an immunoglobulin variable\ndomain sequence analysis tool","volume":"41","author":"Ye","year":"2013","journal-title":"Nucleic Acids\nRes."}],"container-title":["ACS Omega"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsomega.9b00374","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/acsodf\/article-pdf\/4\/4\/6808\/68368238\/acsomega.9b00374.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/acsodf\/article-pdf\/4\/4\/6808\/68368238\/acsomega.9b00374.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T11:26:50Z","timestamp":1788521210000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/acsodf\/article\/4\/4\/6808\/607132\/Highly-Sensitive-and-Selective-Direct-Detection-of"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4,15]]},"references-count":37,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2019,4,15]]},"published-print":{"date-parts":[[2019,4,30]]}},"URL":"https:\/\/doi.org\/10.1021\/acsomega.9b00374","relation":{},"ISSN":["2470-1343"],"issn-type":[{"value":"2470-1343","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,4,15]]}}}