{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T15:18:04Z","timestamp":1788362284763,"version":"build-2803163510"},"reference-count":29,"publisher":"American Chemical Society (ACS)","issue":"7","license":[{"start":{"date-parts":[[2019,7,10]],"date-time":"2019-07-10T00:00:00Z","timestamp":1562716800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/pubs.acs.org\/page\/policy\/authorchoice_termsofuse.html"}],"funder":[{"DOI":"10.13039\/501100001843","name":"Science and Engineering Research Board","doi-asserted-by":"publisher","award":["SB\/S1\/OC-94\/2013"],"award-info":[{"award-number":["SB\/S1\/OC-94\/2013"]}],"id":[{"id":"10.13039\/501100001843","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008984","name":"Indian Institute of Technology Kharagpur","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/501100008984","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,7,31]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>We describe a rapid electrophoresis-based method for profiling of carbonic anhydrase inhibitors. In addition to the pharmacophore moiety intended for reversible interaction with a target enzyme, a fluorescent template with a built-in azide group for photoaffinity labeling is also included as a part of the inhibitor design. Following incubation and irradiation, gel electrophoresis with visualization under UV allows assessment of the efficiency of cross-linking. The relative efficiency of cross-linking of various probes can be regarded as a reflection of their inhibition potencies, an assumption supported by the trend in their IC50\/Ki values. The method has the advantage of being applicable to impure enzyme preparations and also can be used to screen several inhibitors including their promiscuity in parallel in a short time as has been currently demonstrated with HCA II.<\/jats:p>","DOI":"10.1021\/acsomega.9b01044","type":"journal-article","created":{"date-parts":[[2019,7,10]],"date-time":"2019-07-10T08:28:57Z","timestamp":1562747337000},"page":"11914-11920","source":"Crossref","is-referenced-by-count":2,"title":["Naphthalimide-Based Template for Inhibitor Screening\nvia Cross-Linking and In-Gel Fluorescence: A Case Study against HCA\nII"],"prefix":"10.1021","volume":"4","author":[{"given":"Monisha","family":"Singha","sequence":"first","affiliation":[{"name":"Indian Institute of Technology Kharagpur , , ,","place":["Kharagpur, India, 721302"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sayantani","family":"Roy","sequence":"additional","affiliation":[{"name":"Indian Institute of Technology Kharagpur , , ,","place":["Kharagpur, India, 721302"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ravina","family":"Moirangthem","sequence":"additional","affiliation":[{"name":"Indian Institute of Technology Kharagpur , , ,","place":["Kharagpur, India, 721302"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amit K.","family":"Das","sequence":"additional","affiliation":[{"name":"Indian Institute of Technology Kharagpur , , ,","place":["Kharagpur, India, 721302"]},{"name":"Indian Institute of Technology Kharagpur , , ,","place":["Kharagpur, India, 721302"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4041-2846","authenticated-orcid":true,"given":"Amit","family":"Basak","sequence":"additional","affiliation":[{"name":"Indian Institute of Technology Kharagpur , , ,","place":["Kharagpur, India, 721302"]},{"name":"Indian Institute of Technology Kharagpur , , ,","place":["Kharagpur, India, 721302"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2019,7,10]]},"reference":[{"key":"2026090210463043500_cit1a","doi-asserted-by":"publisher","first-page":"1239","DOI":"10.1111\/j.1476-5381.2010.01127.x","article-title":"Principles of early drug discovery","volume":"162","author":"Hughes","year":"2011","journal-title":"Br. 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