{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T18:12:51Z","timestamp":1788545571973,"version":"build-2803163510"},"reference-count":16,"publisher":"American Chemical Society (ACS)","issue":"7","license":[{"start":{"date-parts":[[2019,7,29]],"date-time":"2019-07-29T00:00:00Z","timestamp":1564358400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/pubs.acs.org\/page\/policy\/authorchoice_termsofuse.html"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,7,31]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The use of multivalent glycoconjugate vaccines has dramatically contributed to reduce the incidence of meningococcal infectious disease. The advanced structural characterization of polysaccharide conjugates leads to enhancements in the quality and control of the products. Here, we report a novel nuclear magnetic resonance (NMR) method to confirm the identity and structural conformity (e.g., O-acetyl content) of saccharide antigens that comprise a licensed tetravalent meningococcal serogroups A, C, W, and Y vaccine. For the first time, the NMR methodology is applied on a formulation (licensed vaccine) containing a large excess of excipient (i.e., sucrose) without analytical sample pretreatment. This work confirms the applicability of a rapid and easy NMR assay on a multivalent conjugate vaccine, which might be extended to other combination vaccines that are already licensed or in clinical development.<\/jats:p>","DOI":"10.1021\/acsomega.9b01678","type":"journal-article","created":{"date-parts":[[2019,7,29]],"date-time":"2019-07-29T10:06:11Z","timestamp":1564394771000},"page":"12827-12832","source":"Crossref","is-referenced-by-count":8,"title":["NMR Assays for Estimating the\n                    <i>O<\/i>\n                    -Acetyl\nContent of Meningococcal Polysaccharide Serogroup A in Quadrivalent\nConjugate Vaccine Formulation"],"prefix":"10.1021","volume":"4","author":[{"given":"Silvia","family":"Martini","sequence":"first","affiliation":[{"name":"GSK Vaccines S.r.l. , , Via Fiorentina 1 , , ,","place":["Siena, Italy, 53100"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marianna","family":"Aggravi","sequence":"additional","affiliation":[{"name":"GSK Vaccines S.r.l. , , Via Fiorentina 1 , , ,","place":["Siena, Italy, 53100"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simona","family":"Cianetti","sequence":"additional","affiliation":[{"name":"GSK Vaccines S.r.l. , , Via Fiorentina 1 , , ,","place":["Siena, Italy, 53100"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Egan","sequence":"additional","affiliation":[{"name":"GSK Vaccines S.r.l. , , Via Fiorentina 1 , , ,","place":["Siena, Italy, 53100"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malte","family":"Meppen","sequence":"additional","affiliation":[{"name":"GSK Vaccines S.r.l. , , Via Fiorentina 1 , , ,","place":["Siena, Italy, 53100"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessio","family":"Moriconi","sequence":"additional","affiliation":[{"name":"GSK Vaccines S.r.l. , , Via Fiorentina 1 , , ,","place":["Siena, Italy, 53100"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"Simeone","sequence":"additional","affiliation":[{"name":"GSK Vaccines S.r.l. , , Via Fiorentina 1 , , ,","place":["Siena, Italy, 53100"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4906-5644","authenticated-orcid":true,"given":"Francesco","family":"Berti","sequence":"additional","affiliation":[{"name":"GSK Vaccines S.r.l. , , Via Fiorentina 1 , , ,","place":["Siena, Italy, 53100"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2019,7,29]]},"reference":[{"key":"2026090413100909800_cit1","doi-asserted-by":"crossref","unstructured":"Berti, F.\n          \n          Multivalent meningococcal\nconjugate vaccines: chemical conjugation strategies used for the preparation\nof vaccines licensed or in clinical trials. In  Carbohydrate-Based Vaccines: From Concept to Clinic, ACS Symposium\nSeries, 2018; Vol. 1290, chapter\n6, pp 123\u2013137.","DOI":"10.1021\/bk-2018-1290.ch006"},{"key":"2026090413100909800_cit2","doi-asserted-by":"publisher","first-page":"1088","DOI":"10.1016\/s1473-3099(18)30400-6","article-title":"Safety and immunogenicity of a pentavalent\nmeningococcal conjugate vaccine containing serogroups A, C, Y, W,\nand X in healthy adults: a phase 1, single-centre, double-blind, randomised,\ncontrolled study","volume":"18","author":"Chen","year":"2018","journal-title":"Lancet Infect. Dis."},{"key":"2026090413100909800_cit3","doi-asserted-by":"publisher","first-page":"293","DOI":"10.1590\/s0001-37652005000200009","article-title":"Vaccines based\non the cell surface carbohydrates of pathogenic bacteria","volume":"77","author":"Jones","year":"2005","journal-title":"An. Acad. Bras. Cienc."},{"key":"2026090413100909800_cit4","doi-asserted-by":"publisher","first-page":"840","DOI":"10.1016\/j.jpba.2005.01.044","article-title":"NMR assays\nfor carbohydrate-based vaccines","volume":"38","author":"Jones","year":"2005","journal-title":"J. Pharm. Biomed.\nAnal."},{"key":"2026090413100909800_cit5","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1007\/978-1-4939-2874-3_12","article-title":"Characterization\nof carbohydrate vaccines by NMR spectroscopy","volume":"1331","author":"Berti","year":"2015","journal-title":"Methods Mol. Biol."},{"key":"2026090413100909800_cit6","doi-asserted-by":"publisher","first-page":"2284","DOI":"10.1016\/j.vaccine.2008.01.022","article-title":"Physicochemical characterisation\nof glycoconjugate vaccines for prevention of meningococcal diseases","volume":"26","author":"Bardotti","year":"2008","journal-title":"Vaccine"},{"key":"2026090413100909800_cit7","doi-asserted-by":"publisher","first-page":"353","DOI":"10.1016\/j.biologicals.2012.03.003","article-title":"Evaluation\nof a candidate International Standard for Meningococcal Group C polysaccharide","volume":"40","author":"Vipond","year":"2012","journal-title":"Biologicals"},{"key":"2026090413100909800_cit8","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1016\/j.biologicals.2017.03.001","article-title":"MenA\/MenX Is Working Group. Evaluation\nof candidate international standards for meningococcal serogroups\nA and X polysaccharide","volume":"47","author":"Vipond","year":"2017","journal-title":"Biologicals"},{"key":"2026090413100909800_cit9","first-page":"273","volume":"59","author":"Centers for Disease Control and Prevention (CDC).","year":"2010","journal-title":"MMWR Morb. Mortal. Wkly. Rep."},{"key":"2026090413100909800_cit12","doi-asserted-by":"publisher","first-page":"226","DOI":"10.1006\/abio.1999.4470","article-title":"Development and validation of an\nNMR-based identity\nassay for bacterial polysaccharides","volume":"279","author":"Abeygunawardana","year":"2000","journal-title":"Anal. Biochem."},{"key":"2026090413100909800_cit13","doi-asserted-by":"publisher","first-page":"42765","DOI":"10.1074\/jbc.m313552200","article-title":"TheNeisseria meningitidisSerogroup\nA Capsular Polysaccharide\nO-3 and O-4 Acetyltransferase","volume":"279","author":"Gudlavalleti","year":"2004","journal-title":"J. Biol. Chem."},{"key":"2026090413100909800_cit14","doi-asserted-by":"publisher","first-page":"3707","DOI":"10.1128\/iai.70.7.3707-3713.2002","article-title":"Effect\nof O acetylation of Neisseria meningitidis serogroup A capsular polysaccharide\non development of functional immune responses","volume":"70","author":"Berry","year":"2002","journal-title":"Infect. Immun."},{"key":"2026090413100909800_cit15","doi-asserted-by":"publisher","first-page":"1499","DOI":"10.1128\/cvi.00162-13","article-title":"Meningococcal Polysaccharide AO-Acetylation\nLevels\nDo Not Impact the Immunogenicity of the Quadrivalent Meningococcal\nTetanus Toxoid Conjugate Vaccine: Results from a Randomized, Controlled\nPhase III Study of Healthy Adults Aged 18 to 25 Years","volume":"20","author":"Lupisan","year":"2013","journal-title":"Clin. Vaccine Immunol."},{"key":"2026090413100909800_cit16","doi-asserted-by":"publisher","first-page":"1340","DOI":"10.3390\/molecules23061340","article-title":"Role of O-Acetylation in the Immunogenicity\nof Bacterial Polysaccharide Vaccines","volume":"23","author":"Berti","year":"2018","journal-title":"Molecules"},{"key":"2026090413100909800_cit17","first-page":"71","article-title":"Bacterial polysaccharides\nas major surface antigens: interest in O-acetyl substitutions","volume-title":"Carbohydrate Chemistry","author":"Mulard","year":"2018"},{"key":"2026090413100909800_cit19","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1016\/s0008-6215(96)00253-4","article-title":"Full 1H NMR assignment and detailed O-acetylation patterns of capsular\npolysaccharides from Neisseria meningitidis used in vaccine production","volume":"296","author":"Lemercinier","year":"1996","journal-title":"Carbohydr. Res."}],"container-title":["ACS Omega"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsomega.9b01678","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/acsodf\/article-pdf\/4\/7\/12827\/68385163\/acsomega.9b01678.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/acsodf\/article-pdf\/4\/7\/12827\/68385163\/acsomega.9b01678.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T17:33:10Z","timestamp":1788543190000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/acsodf\/article\/4\/7\/12827\/1574454\/NMR-Assays-for-Estimating-the-O-Acetyl-Content-of"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,7,29]]},"references-count":16,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2019,7,29]]},"published-print":{"date-parts":[[2019,7,31]]}},"URL":"https:\/\/doi.org\/10.1021\/acsomega.9b01678","relation":{},"ISSN":["2470-1343"],"issn-type":[{"value":"2470-1343","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,7,29]]}}}