{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T04:23:44Z","timestamp":1789791824110,"version":"4.0.1"},"reference-count":32,"publisher":"American Chemical Society (ACS)","issue":"15","license":[{"start":{"date-parts":[[2019,9,27]],"date-time":"2019-09-27T00:00:00Z","timestamp":1569542400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/pubs.acs.org\/page\/policy\/authorchoice_termsofuse.html"}],"funder":[{"DOI":"10.13039\/na","name":"Research and Education Center for Robust Agriculture, Forestry and Fisheries Industry, Hokkaido University","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,10,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Avian influenza virus (AIV) infection, caused by influenza virus type A, is an infectious, acute respiratory disease of birds related to influenza outbreaks worldwide. The highly pathogenic AIV subtype H5N1 has crossed species barriers to infect mammals, including humans, with fatal outcomes and has received attention as a potential pandemic threat. A rapid and timely detection in poultry is vitally important to prevent the virus spread. Despite their great sensitivity, conventional detection methods such as real-time reverse transcription-polymerase chain reaction and the agar gel precipitation test are time-consuming and labor-intensive and require special training. In this work, an immunowall device was evaluated as an easier and faster way for detecting AIV H5-hemagglutinin (AIV H5-HA). For detection, fluorescence-labeled or enzyme-labeled antibody was employed as a labeling antibody in a sandwich immunoassay. Both were shown in this paper to be easier and faster assays for detection compared with the conventional enzyme-linked immunosorbent assay (ELISA) kit. In addition, high selectivity was achieved for AIV H5-HA detection after the evaluation of other different HA virus subtypes. The limit of detection was 0.23 ng\/mL for the enzyme-labeled antibody. This value was equivalent to that of the conventional ELISA kit but 8 times faster (31 min compared to 260 min). The detection range was 0.23\u2013100 ng\/mL. The immunowall device with the enzyme-labeled antibody offers a rapid, sensitive, selective, and simple immunoassay system for future H5 AIV real sample detection.<\/jats:p>","DOI":"10.1021\/acsomega.9b02788","type":"journal-article","created":{"date-parts":[[2019,9,27]],"date-time":"2019-09-27T09:43:42Z","timestamp":1569577422000},"page":"16683-16688","source":"Crossref","is-referenced-by-count":26,"title":["Rapid, Sensitive, and Selective Detection of H5 Hemagglutinin\nfrom Avian Influenza Virus Using an Immunowall Device"],"prefix":"10.1021","volume":"4","author":[{"given":"Kenia","family":"Cha\u0301vez Ramos","sequence":"first","affiliation":[{"name":"National Autonomous University of Mexico , , Av. Universidad 3000 , ,","place":["Mexico City, Mexico, 04510"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7433-1019","authenticated-orcid":true,"given":"Keine","family":"Nishiyama","sequence":"additional","affiliation":[{"name":"Hokkaido University , , Kita 13 Nishi 8 , Kita-ku, ,","place":["Sapporo, Japan, 060-8628"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7500-4231","authenticated-orcid":true,"given":"Masatoshi","family":"Maeki","sequence":"additional","affiliation":[{"name":"Hokkaido University , , Kita 13 Nishi 8 , Kita-ku, ,","place":["Sapporo, Japan, 060-8628"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4100-9426","authenticated-orcid":true,"given":"Akihiko","family":"Ishida","sequence":"additional","affiliation":[{"name":"Hokkaido University , , Kita 13 Nishi 8 , Kita-ku, ,","place":["Sapporo, Japan, 060-8628"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirofumi","family":"Tani","sequence":"additional","affiliation":[{"name":"Hokkaido University , , Kita 13 Nishi 8 , Kita-ku, ,","place":["Sapporo, Japan, 060-8628"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshihiro","family":"Kasama","sequence":"additional","affiliation":[{"name":"The University of Tokyo , , Shinkawasaki , , ,","place":["Saiwai-ku, Kanagawa, Japan, 212-0032"]},{"name":"Nagoya University , , Furo-cho , Chikusa-ku, ,","place":["Nagoya, Japan, 464-8603"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshinobu","family":"Baba","sequence":"additional","affiliation":[{"name":"Nagoya University , , Furo-cho , Chikusa-ku, ,","place":["Nagoya, Japan, 464-8603"]},{"name":"Nagoya University , , ,","place":["Nagoya, Japan, 464-8603"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4412-2144","authenticated-orcid":true,"given":"Manabu","family":"Tokeshi","sequence":"additional","affiliation":[{"name":"Hokkaido University , , Kita 13 Nishi 8 , Kita-ku, ,","place":["Sapporo, Japan, 060-8628"]},{"name":"Nagoya University , , Furo-cho , Chikusa-ku, ,","place":["Nagoya, Japan, 464-8603"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2019,9,27]]},"reference":[{"key":"2026090209470730600_cit1","doi-asserted-by":"publisher","first-page":"243","DOI":"10.1128\/cmr.00037-06","article-title":"Avian influenza\nvirus (H5N1): a threat\nto human health","volume":"20","author":"Peiris","year":"2007","journal-title":"Clin. 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Biochem."},{"key":"2026090209470730600_cit3","first-page":"1647","article-title":"Orthomyxoviridae: The viruses\nand their replication","volume-title":"Fields Virology","author":"Palese","year":"2007","edition":"5"},{"key":"2026090209470730600_cit4","doi-asserted-by":"publisher","first-page":"D49","DOI":"10.1016\/j.vaccine.2008.07.039","article-title":"The biology of influenza\nviruses","volume":"26","author":"Bouvier","year":"2008","journal-title":"Vaccine"},{"key":"2026090209470730600_cit5","doi-asserted-by":"publisher","first-page":"1153","DOI":"10.1016\/j.bbamem.2013.10.004","article-title":"Receptor binding\nand\npH stability - How influenza A virus hemagglutinin affects host-specific\nvirus infection","volume":"1838","author":"Mair","year":"2014","journal-title":"Biochim. Biophys. 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Chem."},{"key":"2026090209470730600_cit16","doi-asserted-by":"publisher","first-page":"375","DOI":"10.1002\/rmv.370","article-title":"Molecular diagnosis\nof influenza","volume":"12","author":"Ellis","year":"2002","journal-title":"Rev. Med. Virol."},{"key":"2026090209470730600_cit17","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1016\/j.cimid.2008.01.009","article-title":"Conventional and future diagnostics\nfor avian influenza","volume":"32","author":"Charlton","year":"2009","journal-title":"Comp. Immunol. Microbiol.\nInfect. Dis."},{"key":"2026090209470730600_cit18","doi-asserted-by":"publisher","first-page":"3256","DOI":"10.1128\/jcm.40.9.3256-3260.2002","article-title":"Development\nof a real-time reverse transcriptase PCR assay for type A influenza\nvirus and the avian H5 and H7 hemagglutinin subtypes","volume":"40","author":"Spackman","year":"2002","journal-title":"J. Clin. Microbiol."},{"key":"2026090209470730600_cit19","doi-asserted-by":"publisher","first-page":"113","DOI":"10.7883\/yoken.jjid.2014.136","article-title":"Development of a diagnostic system for novel influenza\nA(H7N9) virus using a real-time RT-PCR assay in Japan","volume":"68","author":"Takayama","year":"2015","journal-title":"Jpn. J. Infect. Dis."},{"key":"2026090209470730600_cit20","doi-asserted-by":"publisher","first-page":"196","DOI":"10.1128\/jcm.39.1.196-200.2001","article-title":"Simultaneous detection of influenza viruses A and B\nusing real-time quantitative PCR","volume":"39","author":"van Elden","year":"2001","journal-title":"J. Clin. Microbiol."},{"key":"2026090209470730600_cit21","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1186\/s12985-015-0300-x","article-title":"Simultaneous\ndetection of novel H7N9 and other influenza A viruses in poultry by\nmultiplex real-time RT-PCR","volume":"12","author":"Xu","year":"2015","journal-title":"Virol. 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Microbiol."},{"key":"2026090209470730600_cit25","doi-asserted-by":"publisher","first-page":"617","DOI":"10.1128\/cvi.00444-06","article-title":"Detection\nof H5 avian influenza viruses\nby antigen-capture enzyme-linked immunosorbent assay using H5-specific\nmonoclonal antibody","volume":"14","author":"He","year":"2007","journal-title":"Clin. Vaccine Immunol."},{"key":"2026090209470730600_cit26","doi-asserted-by":"publisher","first-page":"726","DOI":"10.1128\/cvi.00465-08","article-title":"Rapid Detection\nof H5N1 Subtype Influenza Viruses by Antigen Capture Enzyme-Linked\nImmunosorbent Assay Using H5- and N1-Specific Monoclonal Antibodies","volume":"16","author":"Ho","year":"2009","journal-title":"Clin. Vaccine Immunol."},{"key":"2026090209470730600_cit27","doi-asserted-by":"publisher","first-page":"546","DOI":"10.1016\/j.bios.2014.09.037","article-title":"An impedance\nimmunosensor\nbased on low-cost microelectrodes and specific monoclonal antibodies\nfor rapid detection of avian influenza virus H5N1 in chicken swabs","volume":"67","author":"Lin","year":"2015","journal-title":"Biosens. Bioelectron."},{"key":"2026090209470730600_cit28","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1016\/j.jviromet.2007.09.004","article-title":"Development\nof an antigen-capture ELISA for detection of H7 subtype\navian influenza from experimentally infected chickens","volume":"147","author":"Velumani","year":"2008","journal-title":"J. Virol. Methods"},{"key":"2026090209470730600_cit29","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1080\/15321810701603443","article-title":"Prototype single step\nlateral flow technology for detection of avian influenza virus and\nchicken antibody to avian influenza virus","volume":"28","author":"Nielsen","year":"2007","journal-title":"J.\nImmunoassay Immunochem."},{"key":"2026090209470730600_cit30","doi-asserted-by":"publisher","first-page":"618","DOI":"10.1080\/14686996.2016.1227222","article-title":"An immuno-wall\nmicrodevice\nexhibits rapid and sensitive detection of IDH1-R132H mutation specific\nto grade II and III gliomas","volume":"17","author":"Yamamichi","year":"2016","journal-title":"Sci. Technol. Adv.\nMater."},{"key":"2026090209470730600_cit31","first-page":"305","article-title":"Microfluidic\nImmunoassay Devices\nRealize Next Generation Clinical Diagnostics","volume-title":"Next Generation Point-of-Care Biomedical Sensors Technologies for\nCancer Diagnosis","author":"Kasama","year":"2017"},{"key":"2026090209470730600_cit32","doi-asserted-by":"publisher","first-page":"4589","DOI":"10.1039\/c9an00480g","article-title":"Ultrasensitive detection of disease\nbiomarkers using an immuno-wall device with enzymatic amplification","volume":"144","author":"Nishiyama","year":"2019","journal-title":"Analyst"}],"container-title":["ACS Omega"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsomega.9b02788","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/acsodf\/article-pdf\/4\/15\/16683\/67775972\/acsomega.9b02788.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/acsodf\/article-pdf\/4\/15\/16683\/67775972\/acsomega.9b02788.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T13:47:15Z","timestamp":1788356835000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/acsodf\/article\/4\/15\/16683\/605422\/Rapid-Sensitive-and-Selective-Detection-of-H5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9,27]]},"references-count":32,"journal-issue":{"issue":"15","published-online":{"date-parts":[[2019,9,27]]},"published-print":{"date-parts":[[2019,10,8]]}},"URL":"https:\/\/doi.org\/10.1021\/acsomega.9b02788","relation":{},"ISSN":["2470-1343"],"issn-type":[{"value":"2470-1343","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,9,27]]}}}