{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T23:04:14Z","timestamp":1790377454723,"version":"4.1.0"},"reference-count":59,"publisher":"American Chemical Society (ACS)","issue":"5","license":[{"start":{"date-parts":[[2020,1,10]],"date-time":"2020-01-10T00:00:00Z","timestamp":1578614400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/pubs.acs.org\/page\/policy\/authorchoice_termsofuse.html"}],"funder":[{"DOI":"10.13039\/na","name":"ALSAM Foundation","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,2,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Genetic mutations in Duchenne muscular dystrophy (DMD) gene affecting the expression of dystrophin protein lead to a number of muscle disorders collectively called dystrophinopathies. In addition to muscle dystrophin, mutations in brain-specific dystrophin isoforms, in particular those that are expressed in the brain cortex and Purkinje neurons, result in cognitive impairment associated with DMD. These isoforms carry minor variations in the flanking region of the N-terminal actin-binding domain (ABD1) of dystrophin, which is composed of two calponin-homology (CH) domains in tandem. Determining the effect of these sequence variations is critical for understanding the mechanisms that govern varied symptoms of the disease. We studied the impact of differences in the N-terminal flanking region on the structure and function of dystrophin tandem CH domain isoforms. The amino acid changes did not affect the global structure of the protein but drastically affected the thermodynamic stability, with the muscle isoform more stable than the brain and Purkinje isoforms. Actin binding investigated with actin from different sources (skeletal muscle, smooth muscle, cardiac muscle, and platelets) revealed that the muscle isoform binds to filamentous actin (F-actin) with a lower affinity compared to the brain and Purkinje isoforms, and a similar trend was observed with actin from different sources. In addition, all isoforms showed a higher affinity to smooth muscle actin in comparison to actin from other sources. In conclusion, tandem CH domain isoforms might be using minor sequence variations in the N-terminal flanking regions to modulate their thermodynamic stability and actin-binding function, thus leading to specificity in dystrophin\u2013actin interactions in various tissues.<\/jats:p>","DOI":"10.1021\/acsomega.9b02911","type":"journal-article","created":{"date-parts":[[2020,1,10]],"date-time":"2020-01-10T10:07:26Z","timestamp":1578650846000},"page":"2159-2168","source":"Crossref","is-referenced-by-count":8,"title":["Tissue-Specificity of Dystrophin\u2013Actin Interactions:\nIsoform-Specific Thermodynamic Stability and Actin-Binding Function\nof Tandem Calponin-Homology Domains"],"prefix":"10.1021","volume":"5","author":[{"given":"Vaibhav","family":"Upadhyay","sequence":"first","affiliation":[{"name":"University of Colorado Anschutz Medical Campus , , 12850 East Montview Boulevard, MS C238-V20 , , ,","place":["Aurora, Colorado, United States, 80045"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Swati","family":"Bandi","sequence":"additional","affiliation":[{"name":"University of Colorado Anschutz Medical Campus , , 12850 East Montview Boulevard, MS C238-V20 , , ,","place":["Aurora, Colorado, United States, 80045"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sudipta","family":"Panja","sequence":"additional","affiliation":[{"name":"University of Colorado Anschutz Medical Campus , , 12850 East Montview Boulevard, MS C238-V20 , , ,","place":["Aurora, Colorado, United States, 80045"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Saba","sequence":"additional","affiliation":[{"name":"University of Colorado Anschutz Medical Campus , , 12850 East Montview Boulevard, MS C238-V20 , , ,","place":["Aurora, Colorado, United States, 80045"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8308-5318","authenticated-orcid":true,"given":"Krishna M. G.","family":"Mallela","sequence":"additional","affiliation":[{"name":"University of Colorado Anschutz Medical Campus , , 12850 East Montview Boulevard, MS C238-V20 , , ,","place":["Aurora, Colorado, United States, 80045"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2020,1,10]]},"reference":[{"key":"2026090201514391300_cit1","doi-asserted-by":"publisher","first-page":"687","DOI":"10.1016\/S0140-6736(02)07815-7","article-title":"The muscular\ndystrophies","volume":"359","author":"Emery","year":"2002","journal-title":"Lancet"},{"key":"2026090201514391300_cit2","doi-asserted-by":"publisher","first-page":"731","DOI":"10.1016\/S1474-4422(03)00585-4","article-title":"Dystrophin and mutations:\nOne gene,\nseveral proteins, multiple phenotypes","volume":"2","author":"Muntoni","year":"2003","journal-title":"Lancet\nNeurol."},{"key":"2026090201514391300_cit3","doi-asserted-by":"publisher","first-page":"15069","DOI":"10.1073\/pnas.1008818107","article-title":"Missense mutations\nin dystrophin that trigger muscular dystrophy decrease protein stability\nand lead to cross-\u03b2 aggregates","volume":"107","author":"Singh","year":"2010","journal-title":"Proc.\nNatl. 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