{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T08:43:48Z","timestamp":1790325828299,"version":"4.1.0"},"reference-count":33,"publisher":"American Chemical Society (ACS)","issue":"21","license":[{"start":{"date-parts":[[2020,5,18]],"date-time":"2020-05-18T00:00:00Z","timestamp":1589760000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/pubs.acs.org\/page\/policy\/authorchoice_termsofuse.html"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,6,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Antibacterial resistance remains a major global problem due to frequent prescriptions, leading to significant toxicities. To overcome the limitations of antibiotic therapy, it is highly desirable to provide site-specific delivery of drugs with controlled release. Inspired by the biocompatible, biodegradable, and site-specific mimicking behavior of poly\u00ad(ethylene glycol) (PEG) and poly\u00ad(caprolactone) (PCL), we developed vancomycin-PEG-PCL-PEG conjugates to maximize the pharmacological effects and minimize the side effects. Drug-loaded vancomycin-PEG-PCL-PEG conjugates are influenced by size, shape, surface area, encapsulation efficiency, in vitro drug release, hemolysis assay, cytotoxicity, and antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA) and bacterial kill kinetics. The results demonstrated that vancomycin (VCM) release from PEG-PCL-PEG triblock revealed a biphasic manner. Hemolysis assay showed the nonprescription nature of VCM-PEG-PCL-PEG. Cytotoxicity studies confirmed the biocompatibility of VCM-PEG-PCL-PEG. The in vitro antibacterial results showed enhance activity with minimum inhibitory concentration compared to bare VCM. Molecular dynamics simulation study revealed that binding between VCM and PEG-PCL-PEG by hydrophobic interactions offers molecular encapsulation and steric barrier to drug degradation. This newly developed therapeutic delivery system can offer to enhance activity and delivery VCM against MRSA.<\/jats:p>","DOI":"10.1021\/acsomega.9b04064","type":"journal-article","created":{"date-parts":[[2020,5,18]],"date-time":"2020-05-18T20:09:16Z","timestamp":1589832556000},"page":"11935-11945","source":"Crossref","is-referenced-by-count":62,"title":["Pyramid-Shaped PEG-PCL-PEG Polymeric-Based\nModel Systems for Site-Specific Drug Delivery of Vancomycin with Enhance\nAntibacterial Efficacy"],"prefix":"10.1021","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5277-3430","authenticated-orcid":true,"given":"Sima","family":"Singh","sequence":"first","affiliation":[{"name":"University of KwaZulu-Natal , , Private Bag X54001 , ,","place":["Durban, South Africa, 4000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Majed M","family":"Alrobaian","sequence":"additional","affiliation":[{"name":"Taif University , , ,","place":["Taif, Kingdom of Saudi Arabia, 21974"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nagashekhara","family":"Molugulu","sequence":"additional","affiliation":[{"name":"Monash University , , Jalan Lagoon Selatan, , ,","place":["Bandar Sunway, Selangor, Malaysia, 47500"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5365-6332","authenticated-orcid":true,"given":"Nikhil","family":"Agrawal","sequence":"additional","affiliation":[{"name":"University of KwaZulu-Natal , , Private Bag X54001 , ,","place":["Durban, South Africa, 4000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arshid","family":"Numan","sequence":"additional","affiliation":[{"name":"Fudan University , , ,","place":["Shanghai, China, 200433"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0890-769X","authenticated-orcid":true,"given":"Prashant","family":"Kesharwani","sequence":"additional","affiliation":[{"name":"Jamia Hamdard , , ,","place":["New Delhi, India, 110062"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2020,5,18]]},"reference":[{"key":"2026081721195724400_cit1","doi-asserted-by":"publisher","first-page":"S10","DOI":"10.1093\/cid\/cit613","article-title":"Methicillin-Resistant\nStaphylococcus Aureus: An Evolving Pathogen","author":"Stryjewski","year":"2014","journal-title":"Clin. 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