{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T23:29:51Z","timestamp":1790292591950,"version":"4.1.0"},"reference-count":46,"publisher":"American Chemical Society (ACS)","issue":"15","license":[{"start":{"date-parts":[[2020,4,7]],"date-time":"2020-04-07T00:00:00Z","timestamp":1586217600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/pubs.acs.org\/page\/policy\/authorchoice_termsofuse.html"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["SFB765\/2-2014"],"award-info":[{"award-number":["SFB765\/2-2014"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,4,21]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Multicomponent self-assembly of peptides is a powerful strategy to fabricate novel functional materials with synergetic properties that can be used for several nanobiotechnological applications. In the present study, we used a coassembly strategy to generate an injectable ultrashort bioactive peptide hydrogel formed by mixing a dipeptide hydrogelator with a macrophage attracting short chemotactic peptide ligand. Coassembly does not impede hydrogelation as shown by cryo-transmission electron microscopy (cryo-TEM), scanning electron microscopy, and rheology. Biocompatibility was shown by cytotoxicity assays and confocal microscopy. The hydrogels release the entrapped skin antibiotic ciprofloxacin, among others, in a slow and continuous manner. Such bioinspired advanced functional materials can find applications as wound dressing materials to treat chronic wound conditions like diabetic foot ulcer.<\/jats:p>","DOI":"10.1021\/acsomega.9b04371","type":"journal-article","created":{"date-parts":[[2020,4,7]],"date-time":"2020-04-07T21:36:27Z","timestamp":1586295387000},"page":"8557-8563","source":"Crossref","is-referenced-by-count":30,"title":["Coassembly Generates Peptide Hydrogel with Wound Dressing\nMaterial Properties"],"prefix":"10.1021","volume":"5","author":[{"given":"Chaitanya Kumar","family":"Thota","sequence":"first","affiliation":[{"name":"Freie Universita\u0308t Berlin , , Takustrasse 3 , ,","place":["Berlin, Germany, 14195"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allison A.","family":"Berger","sequence":"additional","affiliation":[{"name":"Freie Universita\u0308t Berlin , , Takustrasse 3 , ,","place":["Berlin, Germany, 14195"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Elomaa","sequence":"additional","affiliation":[{"name":"Freie Universita\u0308t Berlin , , Takustrasse 3 , ,","place":["Berlin, Germany, 14195"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaunxiong","family":"Nie","sequence":"additional","affiliation":[{"name":"Freie Universita\u0308t Berlin , , Takustrasse 3 , ,","place":["Berlin, Germany, 14195"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph","family":"Bo\u0308ttcher","sequence":"additional","affiliation":[{"name":"Freie Universita\u0308t Berlin , , Fabeckstrasse 36a , ,","place":["Berlin, Germany, 14195"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9747-0740","authenticated-orcid":true,"given":"Beate","family":"Koksch","sequence":"additional","affiliation":[{"name":"Freie Universita\u0308t Berlin , , Takustrasse 3 , ,","place":["Berlin, Germany, 14195"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2020,4,7]]},"reference":[{"key":"2026092109074320600_cit1","doi-asserted-by":"publisher","first-page":"1704","DOI":"10.1039\/C8SM02573H","article-title":"Recent Advances of\nSelf-Assembling Peptide-Based Hydrogels for Biomedical Applications","volume":"15","author":"Li","year":"2019","journal-title":"Soft Matter"},{"key":"2026092109074320600_cit2","doi-asserted-by":"publisher","first-page":"640","DOI":"10.1039\/C8CC08163H","article-title":"Designer Aromatic Peptide\nAmphiphiles for Self-Assembly and Enzymatic Display of Proteins with\nMorphology Control","volume":"55","author":"Wakabayashi","year":"2019","journal-title":"Chem. Commun."},{"key":"2026092109074320600_cit3","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fchem.2019.00070","article-title":"Novel Materials\nFrom the Supramolecular Self-Assembly of Short Helical \u03923-Peptide\nFoldamers","volume":"7","author":"Kulkarni","year":"2019","journal-title":"Front. Chem."},{"key":"2026092109074320600_cit4","doi-asserted-by":"publisher","first-page":"9117","DOI":"10.1039\/C3RA40234G","article-title":"Peptide Hydrogels","volume":"3","author":"Dasgupta","year":"2013","journal-title":"RSC Adv."},{"key":"2026092109074320600_cit5","doi-asserted-by":"publisher","first-page":"2780","DOI":"10.1039\/b921448h","article-title":"Designer Self-Assembling\nPeptide Nanofiber Biological\nMaterials","volume":"39","author":"Hauser","year":"2010","journal-title":"Chem. Soc. Rev."},{"key":"2026092109074320600_cit6","doi-asserted-by":"publisher","first-page":"6881","DOI":"10.1039\/C4CS00164H","article-title":"The Physical Properties of Supramolecular Peptide Assemblies: From\nBuilding Block Association to Technological Applications","volume":"43","author":"Adler-Abramovich","year":"2014","journal-title":"Chem. Soc. Rev."},{"key":"2026092109074320600_cit7","doi-asserted-by":"publisher","first-page":"8263","DOI":"10.1021\/acsnano.6b00646","article-title":"Water-Floating\nGiant Nanosheets from\nHelical Peptide Pentamers","volume":"10","author":"Lee","year":"2016","journal-title":"ACS Nano"},{"key":"2026092109074320600_cit8","doi-asserted-by":"publisher","first-page":"3665","DOI":"10.1038\/ncomms4665","article-title":"Tyrosine-Mediated Two-Dimensional\nPeptide Assembly\nand Its Role as a Bio-Inspired Catalytic Scaffold","volume":"5","author":"Jang","year":"2014","journal-title":"Nat. Commun."},{"key":"2026092109074320600_cit9","doi-asserted-by":"publisher","first-page":"31167","DOI":"10.1038\/srep31167","article-title":"A Novel\nHighly Stable and Injectable\nHydrogel Based on a Conformationally Restricted Ultrashort Peptide","volume":"6","author":"Thota","year":"2016","journal-title":"Sci. Rep."},{"key":"2026092109074320600_cit10","doi-asserted-by":"publisher","first-page":"1671","DOI":"10.1016\/j.actbio.2013.08.013","article-title":"Self-Assembly of Short\nPeptides to Form Hydrogels: Design of Building\nBlocks, Physical Properties and Technological Applications","volume":"10","author":"Fichman","year":"2014","journal-title":"Acta Biomater."},{"key":"2026092109074320600_cit11","doi-asserted-by":"publisher","first-page":"8047","DOI":"10.1039\/C5CE00801H","article-title":"Hydrogels\nFormed from Fmoc Amino Acids","volume":"17","author":"Draper","year":"2015","journal-title":"CrystEngComm"},{"key":"2026092109074320600_cit12","doi-asserted-by":"publisher","first-page":"701","DOI":"10.1038\/nchembio.1002","article-title":"Phenylalanine Assembly\ninto Toxic Fibrils Suggests Amyloid Etiology in Phenylketonuria","volume":"8","author":"Adler-Abramovich","year":"2012","journal-title":"Nat. Chem. Biol."},{"key":"2026092109074320600_cit13","doi-asserted-by":"publisher","first-page":"8105","DOI":"10.1039\/C5CE01051A","article-title":"Synergetic Functional\nProperties of Two-Component Single Amino Acid-Based Hydrogels","volume":"17","author":"Fichman","year":"2015","journal-title":"CrystEngComm"},{"key":"2026092109074320600_cit14","doi-asserted-by":"publisher","first-page":"2646","DOI":"10.1021\/bm900584m","article-title":"Self-Assembled\nFmoc-Peptides as a\nPlatform for the Formation of Nanostructures and Hydrogels","volume":"10","author":"Orbach","year":"2009","journal-title":"Biomacromolecules"},{"key":"2026092109074320600_cit15","doi-asserted-by":"publisher","first-page":"3220","DOI":"10.1039\/C0SM00018C","article-title":"The Influence\nof Side-Chain Halogenation\non the Self-Assembly and Hydrogelation of Fmoc-Phenylalanine Derivatives","volume":"6","author":"Ryan","year":"2010","journal-title":"Soft Matter"},{"key":"2026092109074320600_cit16","doi-asserted-by":"publisher","first-page":"3464","DOI":"10.1039\/c0cs00032a","article-title":"More than Just Bare\nScaffolds: Towards Multi-Component\nand Decorated Fibrous Biomaterials","volume":"39","author":"Woolfson","year":"2010","journal-title":"Chem. Soc.\nRev."},{"key":"2026092109074320600_cit17","doi-asserted-by":"publisher","first-page":"1450","DOI":"10.3390\/molecules24081450","article-title":"Using Self-Assembling\nPeptides to Integrate Biomolecules\ninto Functional Supramolecular Biomaterials","volume":"24","author":"Liu","year":"2019","journal-title":"Molecules"},{"key":"2026092109074320600_cit18","doi-asserted-by":"publisher","first-page":"70","DOI":"10.1186\/s12951-017-0300-7","article-title":"Nanoscale Click-Reactive\nScaffolds from Peptide Self-Assembly","volume":"15","author":"Guttenplan","year":"2017","journal-title":"J. Nanobiotechnol."},{"key":"2026092109074320600_cit19","doi-asserted-by":"publisher","first-page":"172040","DOI":"10.1098\/rsos.172040","article-title":"Self-Assembly Behaviours of Peptide\u2013Drug Conjugates:\nInfluence of Multiple Factors on Aggregate Morphology and Potential\nSelf-Assembly Mechanism","volume":"5","author":"Fan","year":"2018","journal-title":"R. Soc. Open Sci."},{"key":"2026092109074320600_cit20","doi-asserted-by":"publisher","first-page":"12474","DOI":"10.1021\/acsami.8b01725","article-title":"Cooperative Assembly of a Peptide\nGelator and Silk Fibroin Afford an Injectable Hydrogel for Tissue\nEngineering","volume":"10","author":"Cheng","year":"2018","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"2026092109074320600_cit21","doi-asserted-by":"publisher","first-page":"9586","DOI":"10.1039\/c7cc04187j","article-title":"Molecular Co-Assembly as a Strategy\nfor Synergistic Improvement of\nthe Mechanical Properties of Hydrogels","volume":"53","author":"Halperin-Sternfeld","year":"2017","journal-title":"Chem.\nCommun."},{"key":"2026092109074320600_cit22","doi-asserted-by":"publisher","first-page":"3406","DOI":"10.1039\/c7cs00827a","article-title":"Minimalistic Peptide\nSupramolecular Co-Assembly: Expanding the Conformational\nSpace for Nanotechnology","volume":"47","author":"Makam","year":"2018","journal-title":"Chem. Soc. Rev."},{"key":"2026092109074320600_cit23","doi-asserted-by":"publisher","first-page":"586","DOI":"10.1021\/acscentsci.7b00115","article-title":"Molecular, Local, and Network-Level Basis for the Enhanced\nStiffness of Hydrogel Networks Formed from Coassembled Racemic Peptides:\nPredictions from Pauling and Corey","volume":"3","author":"Nagy-Smith","year":"2017","journal-title":"ACS Cent.\nSci."},{"key":"2026092109074320600_cit24","doi-asserted-by":"publisher","first-page":"28","DOI":"10.1021\/bc070066k","article-title":"Optimizing\nSize and Copy Number for\nPEG-FMLF (N-Formyl-Methionyl-Leucyl-Phenylalanine) Nanocarrier Uptake\nby Macrophages","volume":"19","author":"Wan","year":"2008","journal-title":"Bioconjugate Chem."},{"key":"2026092109074320600_cit25","doi-asserted-by":"publisher","first-page":"216","DOI":"10.1021\/bc0100657","article-title":"Conjugates Bearing Multiple Formyl-Methionyl Peptides Display Enhanced\nBinding to but Not Activation of Phagocytic Cells","volume":"13","author":"Pooyan","year":"2002","journal-title":"Bioconjugate Chem."},{"key":"2026092109074320600_cit26","doi-asserted-by":"publisher","first-page":"2110","DOI":"10.1007\/s11095-007-9402-5","article-title":"Peritoneal Macrophage\nUptake, Pharmacokinetics\nand Biodistribution of Macrophage-Targeted PEG-FMLF (N-Formyl-Methionyl-Leucyl-Phenylalanine)\nNanocarriers for Improving HIV Drug Delivery","volume":"24","author":"Wan","year":"2007","journal-title":"Pharm. Res."},{"key":"2026092109074320600_cit27","doi-asserted-by":"publisher","first-page":"128","DOI":"10.1016\/j.biomaterials.2017.04.042","article-title":"Biomaterials A Macrophage-Activating\n, Injectable Hydrogel to Sequester\nEndogenous Growth Factors for in Situ Angiogenesis","volume":"134","author":"Feng","year":"2017","journal-title":"Biomaterials"},{"key":"2026092109074320600_cit28","doi-asserted-by":"publisher","first-page":"20976","DOI":"10.1073\/pnas.1115973108","article-title":"Dextran Hydrogel Scaffolds Enhance\nAngiogenic Responses and Promote Complete Skin Regeneration during\nBurn Wound Healing","volume":"108","author":"Sun","year":"2011","journal-title":"Proc. Natl. Acad. Sci. U.\nS. A."},{"key":"2026092109074320600_cit29","doi-asserted-by":"publisher","first-page":"7533","DOI":"10.1039\/C7RA13510F","article-title":"A Functional\nChitosan-Based\nHydrogel as a Wound Dressing and Drug Delivery System in the Treatment\nof Wound Healing","volume":"8","author":"Liu","year":"2018","journal-title":"RSC Adv."},{"key":"2026092109074320600_cit30","doi-asserted-by":"publisher","first-page":"655","DOI":"10.1038\/nrd4363","article-title":"Overcoming the Challenges in Administering\nBiopharmaceuticals: Formulation and Delivery Strategies","volume":"13","author":"Mitragotri","year":"2014","journal-title":"Nat. Rev. Drug Discovery"},{"key":"2026092109074320600_cit31","doi-asserted-by":"publisher","first-page":"318","DOI":"10.1016\/j.ejpb.2015.06.008","article-title":"Novel Biodegradable\nPolymers for Local Growth Factor Delivery","volume":"97","author":"Amsden","year":"2015","journal-title":"Eur.\nJ. Pharm. Biopharm."},{"key":"2026092109074320600_cit32","doi-asserted-by":"publisher","first-page":"e73","DOI":"10.1161\/01.CIR.102.11.e73","article-title":"Clinical Trials in Coronary\nAngiogenesis: Issues,\nProblems, Consensus","volume":"102","author":"Simons","year":"2000","journal-title":"Circulation"},{"key":"2026092109074320600_cit33","doi-asserted-by":"publisher","first-page":"1386","DOI":"10.1021\/acsbiomaterials.8b00031","article-title":"Multidomain\nPeptide Hydrogel Accelerates\nHealing of Full-Thickness Wounds in Diabetic Mice","volume":"4","author":"Carrejo","year":"2018","journal-title":"ACS Biomaterials Science & Engineering"},{"key":"2026092109074320600_cit34","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1016\/j.actbio.2018.01.045","article-title":"Nanoengineered Injectable Hydrogels\nfor Wound Healing Application","volume":"70","author":"Lokhande","year":"2018","journal-title":"Acta Biomater."},{"key":"2026092109074320600_cit35","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1038\/s41427-018-0103-9","article-title":"An Injectable\nSelf-Healing Coordinative Hydrogel with Antibacterial\nand Angiogenic Properties for Diabetic Skin Wound Repair","volume":"11","author":"Chen","year":"2019","journal-title":"NPG Asia Mater."},{"key":"2026092109074320600_cit36","doi-asserted-by":"publisher","first-page":"15030","DOI":"10.1021\/ja027993g","article-title":"Responsive\nHydrogels from the Intramolecular\nFolding and Self-Assembly of a Designed Peptide","volume":"124","author":"Schneider","year":"2002","journal-title":"J. Am. Chem. Soc."},{"key":"2026092109074320600_cit37","doi-asserted-by":"publisher","first-page":"5143","DOI":"10.1039\/C0SM00642D","article-title":"Injectable Solid\nHydrogel: Mechanism of Shear-Thinning\nand Immediate Recovery of Injectable \u03b2-Hairpin Peptide Hydrogels","volume":"6","author":"Yan","year":"2010","journal-title":"Soft Matter"},{"key":"2026092109074320600_cit38","doi-asserted-by":"publisher","first-page":"1822","DOI":"10.1002\/adfm.201505188","article-title":"Enzyme-Catalyzed Formation\nof Supramolecular Hydrogels as Promising Vaccine Adjuvants","volume":"26","author":"Wang","year":"2016","journal-title":"Adv. Funct. Mater."},{"key":"2026092109074320600_cit39","doi-asserted-by":"publisher","first-page":"8607","DOI":"10.1039\/C7TB01883E","article-title":"Self-Assembled RGD Dehydropeptide\nHydrogels for Drug Delivery Applications","volume":"5","author":"Vilac\u0327a","year":"2017","journal-title":"J.\nMater. Chem. B"},{"key":"2026092109074320600_cit40","doi-asserted-by":"publisher","first-page":"3621","DOI":"10.1021\/acs.biomac.7b01006","article-title":"Amphiphilic\nPeptide-Based Supramolecular, Noncytotoxic, Stimuli-Responsive Hydrogels\nwith Antibacterial Activity","volume":"18","author":"Nandi","year":"2017","journal-title":"Biomacromolecules"},{"key":"2026092109074320600_cit41","doi-asserted-by":"publisher","first-page":"6798","DOI":"10.1039\/C5TB00842E","article-title":"A Laminin\nMimetic Peptide SIKVAV-Conjugated Chitosan Hydrogel Promoting Wound\nHealing by Enhancing Angiogenesis, Re-Epithelialization and Collagen\nDeposition","volume":"3","author":"Chen","year":"2015","journal-title":"J. Mater. Chem. B"},{"key":"2026092109074320600_cit42","doi-asserted-by":"publisher","first-page":"15040","DOI":"10.1002\/anie.201806483","article-title":"Design\nof a Multicompartment Hydrogel That Facilitates Time-Resolved Delivery\nof Combination Therapy and Synergized Killing of Glioblastoma","volume":"57","author":"Majumder","year":"2018","journal-title":"Angew. Chem., Int. Ed."},{"key":"2026092109074320600_cit43","doi-asserted-by":"publisher","first-page":"1750","DOI":"10.1021\/acscentsci.9b00501","article-title":"Electrostatically Driven Guanidinium Interaction Domains\nThat Control Hydrogel-Mediated Protein Delivery In Vivo","volume":"5","author":"Miller","year":"2019","journal-title":"ACS Cent. Sci."},{"key":"2026092109074320600_cit44","doi-asserted-by":"publisher","first-page":"2672","DOI":"10.1021\/acs.biomac.5b00541","article-title":"Beta Hairpin\nPeptide Hydrogels as an Injectable Solid Vehicle for Neurotrophic\nGrowth Factor Delivery","volume":"16","author":"Lindsey","year":"2015","journal-title":"Biomacromolecules"},{"key":"2026092109074320600_cit45","doi-asserted-by":"publisher","first-page":"11787","DOI":"10.1021\/jp904251j","article-title":"Tetrapeptide-Based\nHydrogels: For\nEncapsulation and Slow Release of an Anticancer Drug at Physiological\nPH","volume":"113","author":"Naskar","year":"2009","journal-title":"J. Phys. Chem. B"},{"key":"2026092109074320600_cit46","doi-asserted-by":"publisher","first-page":"929","DOI":"10.1021\/la4043638","article-title":"Assembly of an Injectable Noncytotoxic\nPeptide-Based Hydrogelator for Sustained Release of Drugs","volume":"30","author":"Baral","year":"2014","journal-title":"Langmuir"}],"container-title":["ACS Omega"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsomega.9b04371","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/acsodf\/article-pdf\/5\/15\/8557\/67801737\/acsomega.9b04371.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/acsodf\/article-pdf\/5\/15\/8557\/67801737\/acsomega.9b04371.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T13:29:58Z","timestamp":1789997398000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/acsodf\/article\/5\/15\/8557\/619450\/Coassembly-Generates-Peptide-Hydrogel-with-Wound"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,7]]},"references-count":46,"journal-issue":{"issue":"15","published-online":{"date-parts":[[2020,4,7]]},"published-print":{"date-parts":[[2020,4,21]]}},"URL":"https:\/\/doi.org\/10.1021\/acsomega.9b04371","relation":{},"ISSN":["2470-1343"],"issn-type":[{"value":"2470-1343","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4,7]]}}}