{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T16:27:03Z","timestamp":1786724823538,"version":"build-2736575974"},"reference-count":40,"publisher":"American Chemical Society (ACS)","issue":"8","license":[{"start":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:00:00Z","timestamp":1785283200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:00:00Z","timestamp":1785283200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:00:00Z","timestamp":1785283200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100005088","name":"Beijing Municipal Health Commission","doi-asserted-by":"publisher","award":["BJRITO-RDP-2025"],"award-info":[{"award-number":["BJRITO-RDP-2025"]}],"id":[{"id":"10.13039\/501100005088","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81801114"],"award-info":[{"award-number":["81801114"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82271260"],"award-info":[{"award-number":["82271260"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Beijing Jishuitan Hospital Nova Program","award":["XKXX202212"],"award-info":[{"award-number":["XKXX202212"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Objective: Accurate measurement of full-length parathyroid hormone (PTH) 1\u201384 is important for evaluating disorders of calcium-phosphate metabolism, particularly in chronic kidney disease (CKD). Because immunoassay results may vary according to assay design and analyte recognition, we developed and validated a liquid chromatography-tandem mass spectrometry (LC-MS\/MS) method for selective quantification of serum PTH 1\u201384 and explored its potential clinical relevance in kidney-related disease. Methods: Serum samples were processed by solid-phase extraction and spiked with an isotope-labeled internal standard. PTH 1\u201384 was separated on a C4 column and detected in positive electrospray ionization mode using multiple reaction monitoring. Analytical performance, including selectivity, linearity, accuracy, precision, recovery, and matrix effect, was evaluated using predefined acceptance criteria. A retrospective clinical analysis was then performed to compare this method with chemiluminescent immunoassay (CLIA) and to assess associations with calcium-phosphate metabolism markers. Results: The assay showed satisfactory analytical performance with negligible matrix effect and no meaningful interference under the tested conditions. In uremic patients, PTH concentrations measured by LC-MS\/MS were generally lower than those obtained by CLIA, although the two methods remained correlated. PTH 1\u201384 measured by LC-MS\/MS showed a stronger association with serum phosphate than with serum calcium in this cohort. In patients with renal involvement, both methods showed higher PTH concentrations than in patients without renal involvement. Conclusion: This LC-MS\/MS assay provides selective quantification of serum full-length PTH 1\u201384 with satisfactory analytical performance. It may serve as an orthogonal analytical approach for studies of mineral metabolism in CKD-related disease, although intermethod differences should be interpreted cautiously.<\/jats:p>","DOI":"10.1021\/acsptsci.5c00748","type":"journal-article","created":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T13:17:01Z","timestamp":1785849421000},"page":"2151-2161","source":"Crossref","is-referenced-by-count":0,"title":["Validation and Clinical Evaluation of an LC-MS\/MS\nMethod for Serum Full-Length Parathyroid Hormone 1\u201384 with\nApplication to Chronic Kidney Disease"],"prefix":"10.1021","volume":"9","author":[{"given":"Chunyan","family":"Li","sequence":"first","affiliation":[{"name":"Capital Medical University , , ,","place":["Beijing, China, 100035"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shangqi","family":"Yin","sequence":"additional","affiliation":[{"name":"Capital Medical University , , ,","place":["Beijing, China, 100035"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiru","family":"Zhao","sequence":"additional","affiliation":[{"name":"Capital Medical University , , ,","place":["Beijing, China, 100035"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruichen","family":"Liu","sequence":"additional","affiliation":[{"name":"Capital Medical University , , ,","place":["Beijing, China, 100035"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianglong","family":"Zhao","sequence":"additional","affiliation":[{"name":"Shanghai AB Sciex Analytical Instrument Trading Co., Ltd. , ,","place":["Beijing, China, 100035"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shanghai AB Sciex Analytical Instrument Trading Co., Ltd. , ,","place":["Beijing, China, 100035"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9481-0797","authenticated-orcid":true,"given":"Fan","family":"Liu","sequence":"additional","affiliation":[{"name":"Peking University , , ,","place":["Beijing, China, 100035"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Yang","sequence":"additional","affiliation":[{"name":"Chinese Academy of Medical Sciences & Peking Union Medical College , , ,","place":["Beijing, China, 100005"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Wu","sequence":"additional","affiliation":[{"name":"Capital Medical University , , ,","place":["Beijing, China, 100035"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,7,29]]},"reference":[{"issue":"4","key":"2026081411072701000_cit1","doi-asserted-by":"publisher","first-page":"316","DOI":"10.1007\/BF00291648","article-title":"Mapping\nparathyroid hormone, beta-globin, insulin,\nand LDH-A genes within the human chromosome 11 short arm by spot blotting\nsorted chromosomes","volume":"69","author":"Lebo","year":"1985","journal-title":"Hum. Genet."},{"issue":"4","key":"2026081411072701000_cit2","doi-asserted-by":"publisher","first-page":"743","DOI":"10.1016\/j.ecl.2018.07.003","article-title":"Physiology\nof Parathyroid Hormone","volume":"47","author":"Goltzman","year":"2018","journal-title":"Endocrinol. Metab. Clin.\nNorth Am."},{"issue":"7","key":"2026081411072701000_cit3","doi-asserted-by":"publisher","first-page":"602","DOI":"10.1001\/jamasurg.2013.104","article-title":"Factors that influence\nparathyroid\nhormone half-life: determining if new intraoperative criteria are\nneeded","volume":"148","author":"Leiker","year":"2013","journal-title":"JAMA Surg."},{"issue":"2","key":"2026081411072701000_cit4","doi-asserted-by":"publisher","first-page":"449","DOI":"10.1172\/JCI110053","article-title":"Metabolism of parathyroid\nhormone by isolated rat Kupffer cells and hepatocytes","volume":"67","author":"Segre","year":"1981","journal-title":"J. Clin. Invest."},{"issue":"5","key":"2026081411072701000_cit5","doi-asserted-by":"publisher","first-page":"1075","DOI":"10.1210\/jcem-63-5-1075","article-title":"Calcium-dependent\nrelease of carboxyl-terminal fragments\nof parathyroid hormone by hyperplastic human parathyroid tissue in\nvitro","volume":"63","author":"Hanley","year":"1986","journal-title":"J. Clin. Endocrinol. Metab."},{"issue":"1","key":"2026081411072701000_cit6","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1210\/endo-117-1-127","article-title":"Metabolism of radioiodinated carboxy-terminal\nfragments of bovine parathyroid hormone in normal and anephric rats","volume":"117","author":"D\u2019Amour","year":"1985","journal-title":"Endocrinology"},{"issue":"5","key":"2026081411072701000_cit7","doi-asserted-by":"publisher","first-page":"1636","DOI":"10.1021\/ac051711o","article-title":"Identification\nof carboxyl-terminal peptide fragments of parathyroid hormone in human\nplasma at low-picomolar levels by mass spectrometry","volume":"78","author":"Zhang","year":"2006","journal-title":"Anal. Chem."},{"issue":"102","key":"2026081411072701000_cit8","doi-asserted-by":"publisher","first-page":"S29","DOI":"10.1038\/sj.ki.5001599","article-title":"Circulating\nPTH molecular forms: what we know and what we don\u2019t","volume":"70","author":"D\u2019Amour","year":"2006","journal-title":"Kidney Int."},{"issue":"11","key":"2026081411072701000_cit9","doi-asserted-by":"publisher","first-page":"860","DOI":"10.1016\/j.tem.2019.07.011","article-title":"PTH\/PTHrP\nReceptor Signaling, Allostery, and Structures","volume":"30","author":"Sutkeviciute","year":"2019","journal-title":"Trends Endocrinol. Metab."},{"issue":"1","key":"2026081411072701000_cit10","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.kisu.2017.04.001","article-title":"KDIGO\n2017 Clinical Practice Guideline Update for the Diagnosis, Evaluation,\nPrevention, and Treatment of Chronic Kidney Disease-Mineral and Bone\nDisorder (CKD-MBD) Foreword","volume":"7","author":"Kidney Dis Improving Global","year":"2017","journal-title":"Kidney Int. Suppl."},{"issue":"6","key":"2026081411072701000_cit11","doi-asserted-by":"publisher","first-page":"1468","DOI":"10.1210\/er.2018-00220","article-title":"Clinical Guidelines and PTH Measurement:\nDoes Assay Generation Matter?","volume":"40","author":"Smit","year":"2019","journal-title":"Endocr. Rev."},{"issue":"5","key":"2026081411072701000_cit12","doi-asserted-by":"publisher","first-page":"463","DOI":"10.1007\/BF03346392","article-title":"Lessons from second- and third-generation\nparathyroid hormone assays in primary hyperparathyroidism","volume":"31","author":"Souberbielle","year":"2008","journal-title":"J. Endocrinol. Invest."},{"issue":"4","key":"2026081411072701000_cit13","doi-asserted-by":"publisher","first-page":"805","DOI":"10.1093\/clinchem\/44.4.805","article-title":"A non-(1\u201384)\ncirculating parathyroid hormone\n(PTH) fragment interferes significantly with intact PTH commercial\nassay measurements in uremic samples","volume":"44","author":"Lepage","year":"1998","journal-title":"Clin.\nChem."},{"issue":"3","key":"2026081411072701000_cit14","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1007\/s40618-016-0553-2","article-title":"How the reference values for serum\nparathyroid hormone\nconcentration are (or should be) established?","volume":"40","author":"Souberbielle","year":"2017","journal-title":"J. Endocrinol. Invest."},{"issue":"1\u20132","key":"2026081411072701000_cit15","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/j.jim.2009.06.003","article-title":"The fundamental\nflaws of immunoassays and potential\nsolutions using tandem mass spectrometry","volume":"347","author":"Hoofnagle","year":"2009","journal-title":"J.\nImmunol. Methods"},{"issue":"9","key":"2026081411072701000_cit16","doi-asserted-by":"publisher","first-page":"1251","DOI":"10.1515\/cclm-2014-0150","article-title":"LC-MS candidate\nreference methods for the harmonisation of parathyroid hormone (PTH)\nmeasurement: a review of recent developments and future considerations","volume":"52","author":"Couchman","year":"2014","journal-title":"Clin. Chem. Lab. Med."},{"issue":"2","key":"2026081411072701000_cit17","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1373\/clinchem.2009.137323","article-title":"Selected\nReaction Monitoring\u2013Mass\nSpectrometric Immunoassay Responsive to Parathyroid Hormone and Related\nVariants","volume":"56","author":"Lopez","year":"2010","journal-title":"Clin. Chem."},{"issue":"3","key":"2026081411072701000_cit18","doi-asserted-by":"publisher","first-page":"907","DOI":"10.1074\/mcp.M113.036095","article-title":"Targeted peptide measurements\nin biology and medicine: best practices for mass spectrometry-based\nassay development using a fit-for-purpose approach","volume":"13","author":"Carr","year":"2014","journal-title":"Mol. Cell Proteomics"},{"key":"2026081411072701000_cit19","volume-title":"Evaluation of the Linearity\nof Quantitative Measurement\nProcedures:A Statistical Approach; Approved Guideline","author":"Clinical and Laboratory Standards Institute.","year":"2020","edition":"Third"},{"key":"2026081411072701000_cit20","doi-asserted-by":"publisher","first-page":"42","DOI":"10.1016\/j.cca.2016.10.016","article-title":"Perspective and priorities for improvement of parathyroid\nhormone (PTH) measurement - A view from the IFCC Working Group for\nPTH","volume":"467","author":"Sturgeon","year":"2017","journal-title":"Clin. Chim. 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Lab. Med."},{"issue":"5","key":"2026081411072701000_cit24","doi-asserted-by":"publisher","first-page":"F975","DOI":"10.1152\/ajprenal.00336.2005","article-title":"PTH(1\u201384)\/PTH(7\u201384):\na balance of power","volume":"290","author":"Friedman","year":"2006","journal-title":"Am. J. Physiol. Renal Physiol."},{"issue":"2","key":"2026081411072701000_cit25","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1007\/s00268-004-7489-y","article-title":"Sequential\nchanges in plasma intact and whole parathyroid hormone levels during\nparathyroidectomy for secondary hyperparathyroidism","volume":"29","author":"Yamashita","year":"2005","journal-title":"World J. Surg."},{"issue":"4","key":"2026081411072701000_cit26","doi-asserted-by":"publisher","first-page":"301","DOI":"10.1530\/eje.0.1490301","article-title":"Large carboxy-terminal\nparathyroid hormone (PTH) fragment with a relatively longer half-life\nthan 1\u201384 PTH is secreted directly from the parathyroid gland\nin humans","volume":"149","author":"Yamashita","year":"2003","journal-title":"Eur. J. Endocrinol."},{"key":"2026081411072701000_cit27","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1016\/j.cca.2020.12.022","article-title":"The path to the standardization of PTH: Is this a realistic\npossibility? a position paper of the IFCC C-BM","volume":"515","author":"Cavalier","year":"2021","journal-title":"Clin. Chim. Acta"},{"issue":"11","key":"2026081411072701000_cit28","doi-asserted-by":"publisher","first-page":"1399","DOI":"10.1093\/clinchem\/hvac135","article-title":"Validation\nof an LC-MS\/MS\nMethod Using Solid-Phase Extraction for the Quantification of 1\u201384\nParathyroid Hormone: Toward a Candidate Reference Measurement Procedure","volume":"68","author":"Farre\u0301-Segura","year":"2022","journal-title":"Clin. Chem."},{"key":"2026081411072701000_cit29","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2025.116678","article-title":"Quantification and clinical\nperformance of\nserum parathyroid hormone 1\u201384 via immunocapture coupled to\nLC-MS\/MS in chronic renal failure","volume":"256","author":"Cao","year":"2025","journal-title":"J. Pharm.\nBiomed. Anal."},{"issue":"4","key":"2026081411072701000_cit30","doi-asserted-by":"publisher","first-page":"442","DOI":"10.1159\/000187861","article-title":"Dialysis membranes\nand PTH changes during hemodialysis in patients with secondary hyperparathyroidism","volume":"66","author":"De Francisco","year":"1994","journal-title":"Nephron"},{"issue":"6","key":"2026081411072701000_cit31","doi-asserted-by":"publisher","first-page":"1283","DOI":"10.2215\/cjn.01520407","article-title":"Dynamics of parathyroid hormone secretion\nin health and secondary hyperparathyroidism","volume":"2","author":"Felsenfeld","year":"2007","journal-title":"Clin. J. Am. Soc. Nephrol."},{"issue":"4","key":"2026081411072701000_cit32","doi-asserted-by":"publisher","first-page":"596","DOI":"10.1016\/S0272-6386(96)90473-4","article-title":"Phosphorus accelerates the development\nof parathyroid hyperplasia and secondary hyperparathyroidism in rats\nwith renal failure","volume":"28","author":"Denda","year":"1996","journal-title":"Am. J. Kidney Dis."},{"issue":"1","key":"2026081411072701000_cit33","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-12399-9","article-title":"Phosphate acts directly\non the calcium-sensing receptor to stimulate parathyroid hormone secretion","volume":"10","author":"Centeno","year":"2019","journal-title":"Nat. Commun."},{"issue":"10","key":"2026081411072701000_cit34","doi-asserted-by":"publisher","first-page":"1794","DOI":"10.1093\/ndt\/gfaa054","article-title":"Impact of\nlonger term phosphorus control on cardiovascular mortality in hemodialysis\npatients using an area under the curve approach: results from the\nDOPPS","volume":"35","author":"Lopes","year":"2020","journal-title":"Nephrol., Dial., Transplant."},{"issue":"5","key":"2026081411072701000_cit35","doi-asserted-by":"publisher","first-page":"1506","DOI":"10.1093\/ndt\/gfn613","article-title":"Systematic review of the evidence underlying the association\nbetween mineral metabolism disturbances and risk of all-cause mortality,\ncardiovascular mortality and cardiovascular events in chronic kidney\ndisease","volume":"24","author":"Covic","year":"2009","journal-title":"Nephrol., Dial., Transplant."},{"issue":"2","key":"2026081411072701000_cit36","doi-asserted-by":"publisher","first-page":"360","DOI":"10.1093\/ndt\/gfs404","article-title":"Control of\nmineral metabolism and bone disease in haemodialysis patients: which\noptimal targets?","volume":"28","author":"Fouque","year":"2013","journal-title":"Nephrol., Dial., Transplant."},{"issue":"1","key":"2026081411072701000_cit37","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1172\/JCI107162","article-title":"Parathyroid function in primary osteoporosis","volume":"52","author":"Riggs","year":"1973","journal-title":"J. Clin. Invest."},{"issue":"8","key":"2026081411072701000_cit38","doi-asserted-by":"publisher","first-page":"540","DOI":"10.1097\/01.rlu.0000170012.06271.76","article-title":"Hyperparathyroidism\naccompanying multiple myeloma","volume":"30","author":"Patel","year":"2005","journal-title":"Clin. Nucl. Med."},{"issue":"12","key":"2026081411072701000_cit39","doi-asserted-by":"publisher","first-page":"770","DOI":"10.1055\/a-1049-0647","article-title":"Diagnosis, Pathophysiology and Management of Hypercalcemia in Malignancy:\nA Review of the Literature","volume":"51","author":"Asonitis","year":"2019","journal-title":"Horm. Metab. Res."},{"key":"2026081411072701000_cit40","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2024.119846","article-title":"Liquid chromatography-tandem\nmass spectrometry in clinical laboratory protein measurement","volume":"562","author":"Ma","year":"2024","journal-title":"Clin. Chim. Acta"}],"container-title":["ACS Pharmacology &amp; Translational Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsptsci.5c00748","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/aptsfn\/article-pdf\/9\/8\/2151\/66329975\/acsptsci.5c00748.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/aptsfn\/article-pdf\/9\/8\/2151\/66329975\/acsptsci.5c00748.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T15:28:13Z","timestamp":1786721293000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/aptsfn\/article\/9\/8\/2151\/5236715\/Validation-and-Clinical-Evaluation-of-an-LC-MS-MS"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,29]]},"references-count":40,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,7,29]]},"published-print":{"date-parts":[[2026,8,14]]}},"URL":"https:\/\/doi.org\/10.1021\/acsptsci.5c00748","relation":{},"ISSN":["2575-9108"],"issn-type":[{"value":"2575-9108","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,29]]}}}