{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T14:09:18Z","timestamp":1789135758164,"version":"build-2803163510"},"reference-count":38,"publisher":"American Chemical Society (ACS)","issue":"9","license":[{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico","doi-asserted-by":"publisher","award":["310635\/2020-9"],"award-info":[{"award-number":["310635\/2020-9"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005667","name":"Funda??o de Amparo ? Pesquisa e Inova??o do Estado de Santa Catarina","doi-asserted-by":"publisher","award":["2024TR002566"],"award-info":[{"award-number":["2024TR002566"]}],"id":[{"id":"10.13039\/501100005667","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Physical exercise recruits endogenous opioid mechanisms in the central nervous system, but whether this physiological opioid tone potentiates the effects of exogenous \u03bc-agonists, and whether such interactions differ between sexes, remains unknown. Here, we used the Straub tail response as a behavioral index of central \u03bc2-opioid receptor activity to investigate whether acute submaximal running modulates the pharmacodynamic effects of meperidine in male and female Swiss mice. In resting animals, meperidine produced a dose- and time-dependent Straub tail response across a 12-fold dose range, and the submaximal doses of 8 and 12 mg\/kg were selected for treadmill experiments. Running at approximately 70% of V\u0307O2 max potentiated the Straub tail response at 12 mg\/kg in both sexes, with the effect substantially larger in females, as indicated by a significant Sex \u00d7 Exercise interaction. Thermal antinociception was equivalent between sexes at 12 mg\/kg, indicating that the dimorphism was specific to the exercise-opioid interaction rather than to baseline drug sensitivity. A separate experiment testing whether running alone elicits a naloxone-sensitive response was inconclusive in both sexes; under opioid receptor blockade, however, females maintained higher running-induced scores than males, indicating that the sex difference persists during naloxone treatment. These findings indicate that acute submaximal running potentiates the Straub tail response to meperidine in a dose- and sex-dependent manner. The sexually dimorphic nature of this interaction suggests that biological sex and exercise intensity may jointly modulate opioid pharmacodynamics during physical activity.<\/jats:p>","DOI":"10.1021\/acsptsci.6c00328","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T01:53:57Z","timestamp":1788400437000},"page":"2617-2627","source":"Crossref","is-referenced-by-count":0,"title":["Acute Submaximal\nRunning Potentiates the Straub Tail\nResponse to Meperidine in a Dose- and Sex-Dependent Manner in Mice"],"prefix":"10.1021","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7187-8756","authenticated-orcid":true,"given":"Tatyana","family":"Nery","sequence":"first","affiliation":[{"name":"UFSC-Federal University of Santa Catarina , , , ,","place":["Ararangua\u0301, SC, Brazil, 88905-120"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5593-7197","authenticated-orcid":true,"given":"Naiara de Souza","family":"Santos","sequence":"additional","affiliation":[{"name":"UFSC-Federal University of Santa Catarina , , , ,","place":["Ararangua\u0301, SC, Brazil, 88905-120"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3941-2953","authenticated-orcid":true,"given":"Ana Cristina de Bem","family":"Alves","sequence":"additional","affiliation":[{"name":"UFSC-Federal University of Santa Catarina , , , ,","place":["Ararangua\u0301, SC, Brazil, 88905-120"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9416-1208","authenticated-orcid":true,"given":"Amanda Leite","family":"Bastos-Pereira","sequence":"additional","affiliation":[{"name":"UFSC-Federal University of Santa Catarina , , , ,","place":["Ararangua\u0301, SC, Brazil, 88905-120"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6825-3333","authenticated-orcid":true,"suffix":"Jr.","given":"Aderbal S.","family":"Aguiar","sequence":"additional","affiliation":[{"name":"UFSC-Federal University of Santa Catarina , , , ,","place":["Ararangua\u0301, SC, Brazil, 88905-120"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,31]]},"reference":[{"key":"2026091109134007500_cit1","doi-asserted-by":"publisher","first-page":"970","DOI":"10.1249\/MSS.0000000000001865","article-title":"The transcriptional signature of a runner\u2019s\nhigh","volume":"51","author":"Hicks","year":"2019","journal-title":"Med. 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Med."},{"key":"2026091109134007500_cit3","doi-asserted-by":"publisher","first-page":"1637","DOI":"10.1016\/0024-3205(81)90319-2","article-title":"The effect\nof running on plasma beta-endorphin","volume":"28","author":"Colt","year":"1981","journal-title":"Life Sci."},{"key":"2026091109134007500_cit4","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1016\/0304-3959(84)90061-7","article-title":"Pain sensitivity, mood\nand plasma endocrine levels in man following long-distance running:\neffects of naloxone","volume":"19","author":"Janal","year":"1984","journal-title":"Pain"},{"key":"2026091109134007500_cit5","doi-asserted-by":"publisher","first-page":"109","DOI":"10.2165\/00007256-198907020-00003","article-title":"Opioids\nand exercise: an update","volume":"7","author":"Sforzo","year":"1989","journal-title":"Sports Med."},{"key":"2026091109134007500_cit6","doi-asserted-by":"publisher","first-page":"2523","DOI":"10.1093\/cercor\/bhn013","article-title":"The runner\u2019s high: opioidergic\nmechanisms in\nthe human brain","volume":"18","author":"Boecker","year":"2008","journal-title":"Cereb. Cortex"},{"key":"2026091109134007500_cit7","doi-asserted-by":"publisher","first-page":"1011","DOI":"10.1126\/science.179.4077.1011","article-title":"Opiate receptor:\ndemonstration in nervous tissue","volume":"179","author":"Pert","year":"1973","journal-title":"Science"},{"key":"2026091109134007500_cit8","doi-asserted-by":"publisher","first-page":"577","DOI":"10.1038\/258577a0","article-title":"Identification of\ntwo related pentapeptides\nfrom the brain with potent opiate agonist activity","volume":"258","author":"Hughes","year":"1975","journal-title":"Nature"},{"key":"2026091109134007500_cit9","doi-asserted-by":"publisher","DOI":"10.1016\/j.npep.2020.102048","article-title":"The patterns of exercise-induced \u03b2-endorphin expression in\nthe central nervous system of rats","volume":"82","author":"Xue","year":"2020","journal-title":"Neuropeptides"},{"key":"2026091109134007500_cit10","doi-asserted-by":"publisher","first-page":"278","DOI":"10.1016\/j.brainresbull.2010.07.009","article-title":"Acute and chronic exercise modulates\nthe expression of MOR opioid receptors in the hippocampal formation\nof rats","volume":"83","author":"de Oliveira","year":"2010","journal-title":"Brain Res. Bull."},{"key":"2026091109134007500_cit11","doi-asserted-by":"publisher","first-page":"940","DOI":"10.1097\/ALN.0b013e318210f880","article-title":"Regular exercise reverses sensory hypersensitivity\nin a rat neuropathic\npain model: role of endogenous opioids","volume":"114","author":"Stagg","year":"2011","journal-title":"Anesthesiology"},{"key":"2026091109134007500_cit12","doi-asserted-by":"publisher","first-page":"1","DOI":"10.15585\/mmwr.rr7103a1","article-title":"CDC clinical\npractice guideline for prescribing opioids for pain \u2500 United\nStates, 2022","volume":"71","author":"Dowell","year":"2022","journal-title":"MMWR Recomm. Rep."},{"key":"2026091109134007500_cit13","first-page":"1462","article-title":"Eine empfindliche\nbiologische Reaktion auf Morphin","volume":"37","author":"Straub","year":"1911","journal-title":"Dtsch. Med.\nWochenschr."},{"key":"2026091109134007500_cit14","doi-asserted-by":"publisher","first-page":"540","DOI":"10.1111\/j.1476-5381.1960.tb00277.x","article-title":"The anatomical\nbasis of the Straub\nphenomenon","volume":"15","author":"Bilbey","year":"1960","journal-title":"Br. J. Pharmacol. Chemother."},{"key":"2026091109134007500_cit15","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1111\/j.1476-5381.1969.tb09500.x","article-title":"Effects of\nopiates and opiate antagonists\non the Straub tail reaction in mice","volume":"36","author":"Aceto","year":"1969","journal-title":"Br. J.\nPharmacol."},{"key":"2026091109134007500_cit16","doi-asserted-by":"publisher","first-page":"249","DOI":"10.1254\/jjp.28.249","article-title":"Effects of catecholaminergic or tryptaminergic\nagents on the morphine-induced Straub tail reaction","volume":"28","author":"Kameyama","year":"1978","journal-title":"Jpn. J. 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Behav."},{"key":"2026091109134007500_cit20","doi-asserted-by":"publisher","first-page":"767","DOI":"10.1016\/S0022-3565(25)21187-9","article-title":"Gender-related differences\nin the\nantinociceptive properties of morphine","volume":"279","author":"Cicero","year":"1996","journal-title":"J. Pharmacol.\nExp. Ther."},{"key":"2026091109134007500_cit21","doi-asserted-by":"publisher","first-page":"375","DOI":"10.1016\/S0149-7634(00)00015-4","article-title":"Sex differences\nin thermal nociception and morphine antinociception in rodents depend\non genotype","volume":"24","author":"Mogil","year":"2000","journal-title":"Neurosci. Biobehav. 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Med."},{"key":"2026091109134007500_cit28","doi-asserted-by":"publisher","first-page":"180","DOI":"10.1002\/ana.410130213","article-title":"Central nervous system excitatory\neffects of meperidine in cancer patients","volume":"13","author":"Kaiko","year":"1983","journal-title":"Ann.\nNeurol."},{"key":"2026091109134007500_cit29","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1097\/00045391-200201000-00010","article-title":"Meperidine: a critical review","volume":"9","author":"Latta","year":"2002","journal-title":"Am. J. Ther."},{"key":"2026091109134007500_cit30","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1016\/j.pbb.2016.06.006","article-title":"Sex differences\nin responsiveness to the prescription opioid oxycodone in mice","volume":"148","author":"Collins","year":"2016","journal-title":"Pharmacol., Biochem. Behav."},{"key":"2026091109134007500_cit31","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-29050-0","article-title":"The exercise sex\ngap and the impact of the estrous\ncycle on exercise performance in mice","volume":"8","author":"Aguiar","year":"2018","journal-title":"Sci.\nRep."},{"key":"2026091109134007500_cit32","doi-asserted-by":"publisher","DOI":"10.3791\/4389","article-title":"Performing vaginal\nlavage, crystal violet staining, and vaginal cytological evaluation\nfor mouse estrous cycle staging identification","volume":"67","author":"McLean","year":"2012","journal-title":"J. Vis. Exp."},{"key":"2026091109134007500_cit33","doi-asserted-by":"publisher","first-page":"e0116000","DOI":"10.1371\/journal.pone.0116000","article-title":"A new device for step-down inhibitory avoidance task\n\u2500 effects of low and high frequency in a novel device for passive\ninhibitory avoidance task that avoids bioimpedance variations","volume":"10","author":"Filho","year":"2015","journal-title":"PLoS One"},{"key":"2026091109134007500_cit34","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1101\/lm.7.1.58","article-title":"Computer-assisted\nbehavioral assessment of Pavlovian fear conditioning in mice","volume":"7","author":"Anagnostaras","year":"2000","journal-title":"Learn. Mem."},{"key":"2026091109134007500_cit35","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-69660-1","article-title":"Neuronal\nadenosine A2A receptors signal ergogenic effects of caffeine","volume":"10","author":"Aguiar","year":"2020","journal-title":"Sci. Rep."},{"key":"2026091109134007500_cit36","doi-asserted-by":"publisher","first-page":"1392","DOI":"10.1097\/00000542-200005000-00030","article-title":"Opiate-induced analgesia\nis increased\nand prolonged in mice lacking P-glycoprotein","volume":"92","author":"Thompson","year":"2000","journal-title":"Anesthesiology"},{"key":"2026091109134007500_cit37","doi-asserted-by":"publisher","first-page":"57568","DOI":"10.1021\/acsomega.5c05266","article-title":"Prior exercise attenuates LPS-induced\nneuroinflammation, dopaminergic dysfunction, and fatigue-like behavior\nin mice","volume":"10","author":"Alves","year":"2025","journal-title":"ACS Omega"},{"key":"2026091109134007500_cit38","unstructured":"Nery, T.; Santos, N. S.; Alves, A. C. B.; Bastos-Pereira, A. L.; Aguiar, A. S., Jr.\n          Straub tail effect of exercise: raw data and analysis\nscripts, version 2,  Open Science Framework, 2026. 10.17605\/OSF.IO\/7Y4T9."}],"container-title":["ACS Pharmacology &amp; Translational\nScience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsptsci.6c00328","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/aptsfn\/article-pdf\/9\/9\/2617\/67976238\/acsptsci.6c00328.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/aptsfn\/article-pdf\/9\/9\/2617\/67976238\/acsptsci.6c00328.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T13:32:54Z","timestamp":1789133574000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/aptsfn\/article\/9\/9\/2617\/5382633\/Acute-Submaximal-Running-Potentiates-the-Straub"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,31]]},"references-count":38,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,8,31]]},"published-print":{"date-parts":[[2026,9,11]]}},"URL":"https:\/\/doi.org\/10.1021\/acsptsci.6c00328","relation":{},"ISSN":["2575-9108"],"issn-type":[{"value":"2575-9108","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,31]]}}}