{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T17:32:38Z","timestamp":1793295158199,"version":"4.5.0"},"reference-count":12,"publisher":"American Chemical Society (ACS)","issue":"6","license":[{"start":{"date-parts":[[2020,4,30]],"date-time":"2020-04-30T00:00:00Z","timestamp":1588204800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2020,4,30]],"date-time":"2020-04-30T00:00:00Z","timestamp":1588204800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2020,4,30]],"date-time":"2020-04-30T00:00:00Z","timestamp":1588204800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/100000185","name":"Defense Advanced Research Projects Agency","doi-asserted-by":"publisher","award":["HR0011-14-C-0075"],"award-info":[{"award-number":["HR0011-14-C-0075"]}],"id":[{"id":"10.13039\/100000185","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006160","name":"Biomass Program","doi-asserted-by":"publisher","award":["EE0007563"],"award-info":[{"award-number":["EE0007563"]}],"id":[{"id":"10.13039\/100006160","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000006","name":"Office of Naval Research","doi-asserted-by":"publisher","award":["N00014-16-1-2558"],"award-info":[{"award-number":["N00014-16-1-2558"]}],"id":[{"id":"10.13039\/100000006","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005562","name":"North Carolina Biotechnology Center","doi-asserted-by":"publisher","award":["2018-BIG-6503"],"award-info":[{"award-number":["2018-BIG-6503"]}],"id":[{"id":"10.13039\/100005562","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["T32GM008555"],"award-info":[{"award-number":["T32GM008555"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["1445726"],"award-info":[{"award-number":["1445726"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,6,19]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>A key challenge in synthetic biology is the successful utilization of characterized parts, such as promoters, in different biological contexts. We report the evaluation of the media robustness of a small library of E.\u00a0coli PhoB regulated promoters that enable heterologous protein production in two-stage cultures. Expression levels were measured both in a rich Autoinduction Broth as well as a minimal mineral salts media. Expression was both media and promoter dependent. Of the 16 promoters tested, 4 were identified to have tightly controlled expression, which was also robust to media formulation. Improved promoter robustness led to more predictable scale up and consistent expression in instrumented bioreactors. This subset of PhoB activated promoters, useful for two-stage autoinduction, highlights the impact of the environment on the performance of biological parts and the importance of robustness testing in synthetic biology.<\/jats:p>","DOI":"10.1021\/acssynbio.0c00182","type":"journal-article","created":{"date-parts":[[2020,4,30]],"date-time":"2020-04-30T18:03:08Z","timestamp":1588269788000},"page":"1483-1486","source":"Crossref","is-referenced-by-count":35,"title":["Media Robustness and Scalability of Phosphate Regulated\nPromoters Useful for Two-Stage Autoinduction in\n                    <i>E.\u00a0coli<\/i>"],"prefix":"10.1021","volume":"9","author":[{"given":"Eirik\nAdim","family":"Moreb","sequence":"first","affiliation":[{"name":"Duke University , , , ,","place":["Durham, North Carolina, United States, 27701"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhixia","family":"Ye","sequence":"additional","affiliation":[{"name":"Duke University , , , ,","place":["Durham, North Carolina, United States, 27701"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John P.","family":"Efromson","sequence":"additional","affiliation":[{"name":"Duke University , , , ,","place":["Durham, North Carolina, United States, 27701"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer N.","family":"Hennigan","sequence":"additional","affiliation":[{"name":"Duke University , , , ,","place":["Durham, North Carolina, United States, 27701"]},{"name":"Duke University , , , ,","place":["Durham, North Carolina, United States, 27701"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romel","family":"Menacho-Melgar","sequence":"additional","affiliation":[{"name":"Duke University , , , ,","place":["Durham, North Carolina, United States, 27701"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8858-9651","authenticated-orcid":true,"given":"Michael D.","family":"Lynch","sequence":"additional","affiliation":[{"name":"Duke University , , , ,","place":["Durham, North Carolina, United States, 27701"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2020,4,30]]},"reference":[{"issue":"7","key":"2026081416111529600_cit1","doi-asserted-by":"publisher","first-page":"787","DOI":"10.1038\/nbt1413","article-title":"Refinement\nand Standardization of\nSynthetic Biological Parts and Devices","volume":"26","author":"Canton","year":"2008","journal-title":"Nat.\nBiotechnol."},{"issue":"7","key":"2026081416111529600_cit2","doi-asserted-by":"publisher","first-page":"771","DOI":"10.1038\/nbt0708-771","article-title":"Setting the\nStandard in Synthetic Biology","volume":"26","author":"Arkin","year":"2008","journal-title":"Nat. Biotechnol."},{"key":"2026081416111529600_cit3","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1016\/j.cels.2017.06.001","article-title":"Biophysical Constraints\nArising from Compositional Context in Synthetic Gene Networks","volume":"5","author":"Yeung","year":"2017","journal-title":"Cell Syst."},{"key":"2026081416111529600_cit4","doi-asserted-by":"publisher","first-page":"856","DOI":"10.1002\/biot.201200085","article-title":"Contextualizing Context for Synthetic\nBiology - Identifying\nCauses of Failure of Synthetic Biological Systems","volume":"7","author":"Cardinale","year":"2012","journal-title":"Biotechnol. J."},{"key":"2026081416111529600_cit5","doi-asserted-by":"crossref","unstructured":"Menacho-Melgar, R., Ye, Z., Moreb, E.\nA., Yang, T., Efromson, J. P., Decker, J. S., and Lynch, M. D. (2020) Improved,\nScalable, Two-Stage, Autoinduction of Recombinant Protein Expression\nin E.\u00a0coli Utilizing Phosphate Depletion. bioRxiv, Jan 5, 2020, 10.1101\/820787 (accessed 2020-02-24).","DOI":"10.1101\/820787"},{"key":"2026081416111529600_cit6","doi-asserted-by":"crossref","unstructured":"Menacho-Melgar, R., Moreb, E. A., Efromson, J. P., and Lynch, M. D. (2020) Improved, Two-Stage Protein Expression and Purification via Autoinduction\nof Both Autolysis and Auto DNA\/RNA Hydrolysis Conferred by Phage Lysozyme\nand DNA\/RNA Endonuclease. bioRxiv, Jan 9, 2020, 10.1101\/2020.01.09.900753 (accessed 2020-02-24).","DOI":"10.1101\/2020.01.09.900753"},{"key":"2026081416111529600_cit7","unstructured":"Warnecke-Lipscomb, T., Lynch, M. D., and Gill, R. T. (2013) Microorganism Production of High-Value Chemical Products, and Related\nCompositions, Methods and Systems. US Patent Application. 20130122541A1."},{"issue":"1","key":"2026081416111529600_cit8","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1111\/j.1574-6968.2006.00440.x","article-title":"Novel Gene Members\nin the Pho Regulon of Escherichia\nColi","volume":"264","author":"Baek","year":"2006","journal-title":"FEMS Microbiol. Lett."},{"issue":"D1","key":"2026081416111529600_cit9","doi-asserted-by":"publisher","first-page":"D212","DOI":"10.1093\/nar\/gky1077","article-title":"RegulonDB v 10.5: Tackling Challenges\nto Unify Classic and High Throughput Knowledge of Gene Regulation\nin E. Coli K-12","volume":"47","author":"Santos-Zavaleta","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"2026081416111529600_cit10","doi-asserted-by":"crossref","unstructured":"Catanach, T. A., McCardell, R., Baetica, A.-A., and Murray, R. M. (2018) Context Dependence of Biological\nCircuits. bioRxiv, July 3, 2018, 10.1101\/360040 (accessed 2020-02-24).","DOI":"10.1101\/360040"},{"issue":"2","key":"2026081416111529600_cit11","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1016\/j.tibtech.2014.11.009","article-title":"Modularity, Context-Dependence, and Insulation in Engineered\nBiological Circuits","volume":"33","author":"Del\nVecchio","year":"2015","journal-title":"Trends Biotechnol."},{"key":"2026081416111529600_cit12","doi-asserted-by":"publisher","first-page":"2006","DOI":"10.1038\/msb4100073","article-title":"Synthetic Biology:\nNew Engineering Rules for an Emerging Discipline","volume":"2","author":"Andrianantoandro","year":"2006","journal-title":"Mol. Syst. 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