{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T14:27:04Z","timestamp":1793370424152,"version":"4.5.0"},"reference-count":43,"publisher":"American Chemical Society (ACS)","issue":"8","license":[{"start":{"date-parts":[[2024,8,2]],"date-time":"2024-08-02T00:00:00Z","timestamp":1722556800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2024,8,2]],"date-time":"2024-08-02T00:00:00Z","timestamp":1722556800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2024,8,2]],"date-time":"2024-08-02T00:00:00Z","timestamp":1722556800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32271533"],"award-info":[{"award-number":["32271533"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013058","name":"Jiangsu Provincial Key Research and Development Program","doi-asserted-by":"publisher","award":["BE2023685"],"award-info":[{"award-number":["BE2023685"]}],"id":[{"id":"10.13039\/501100013058","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,8,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\u03b2-ionone, a norisoprenoid, is a natural aromatic compound derived from plants, which displays various biological activities including anticancer, antioxidant and deworming properties. Due to its large biomass and strong environmental tolerance, the nonconventional oleaginous yeast Candida tropicalis was selected to efficiently synthesize \u03b2-ionone. We initially investigated the capacity of the cytoplasm and subcellular compartments to synthesize \u03b2-ionone independently. Subsequently, through adaptive screening of enzymes, functional identification of subcellular localization signal peptides and subcellular compartment combination strategies, a titer of 152.4 mg\/L of \u03b2-ionone was achieved. Finally, directed evolution of rate-limiting enzyme and overexpression of key enzymes were performed to enhance \u03b2-ionone production. The resulting titer was 400.5 mg\/L in shake flasks and 730 mg\/L in a bioreactor. This study demonstrates the first de novo synthesis of \u03b2-ionone in C. tropicalis, providing a novel cellular chassis for terpenoid fragrances with considerable industrial potential.<\/jats:p>","DOI":"10.1021\/acssynbio.4c00286","type":"journal-article","created":{"date-parts":[[2024,8,2]],"date-time":"2024-08-02T00:13:19Z","timestamp":1722557599000},"page":"2533-2544","source":"Crossref","is-referenced-by-count":22,"title":["Metabolic Engineering of\n                    <i>Candida tropicalis<\/i>\n                    for the\n                    <i>De Novo<\/i>\n                    Synthesis of \u03b2-Ionone"],"prefix":"10.1021","volume":"13","author":[{"given":"Jie","family":"Xu","sequence":"first","affiliation":[{"name":"Jiangnan University , , ,","place":["Wuxi, China, 214122"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1054-2044","authenticated-orcid":true,"given":"Yuanyuan","family":"Xia","sequence":"additional","affiliation":[{"name":"Jiangnan University , , ,","place":["Wuxi, China, 214122"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yibo","family":"Shi","sequence":"additional","affiliation":[{"name":"Jiangnan University , , ,","place":["Wuxi, China, 214122"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manzhi","family":"Zhu","sequence":"additional","affiliation":[{"name":"Jiangnan University , , ,","place":["Wuxi, China, 214122"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haibing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Jiangnan University , , ,","place":["Wuxi, China, 214122"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoying","family":"Gui","sequence":"additional","affiliation":[{"name":"Jiangnan University , , ,","place":["Wuxi, China, 214122"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Shen","sequence":"additional","affiliation":[{"name":"Jiangnan University , , ,","place":["Wuxi, China, 214122"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6173-8787","authenticated-orcid":true,"given":"Haiquan","family":"Yang","sequence":"additional","affiliation":[{"name":"Jiangnan University , , ,","place":["Wuxi, China, 214122"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0695-3382","authenticated-orcid":true,"given":"Xianzhong","family":"Chen","sequence":"additional","affiliation":[{"name":"Jiangnan University , , ,","place":["Wuxi, China, 214122"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2024,8,2]]},"reference":[{"issue":"11","key":"2026102915081876600_cit1","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1691\/ph.2018.8142","article-title":"Cleopatra: From turpentine and juniper\nto ionone and irone","volume":"73","author":"Petroianu","year":"2018","journal-title":"Die Pharmazie-An Int J.\nPharm. 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Chem."},{"issue":"3","key":"2026102915081876600_cit4","doi-asserted-by":"publisher","first-page":"3504","DOI":"10.1104\/pp.104.049718","article-title":"Circadian regulation\nof the PhCCD1 carotenoid cleavage dioxygenase controls emission of\nfl-ionone, a fragrance volatile of petunia flowers","volume":"136","author":"Simkin","year":"2004","journal-title":"Plant Physiol."},{"issue":"6","key":"2026102915081876600_cit5","doi-asserted-by":"publisher","first-page":"882","DOI":"10.1111\/j.1365-313X.2004.02263.x","article-title":"The tomato\ncarotenoid cleavage dioxygenase 1 genes contribute to the formation\nof the flavor volatiles beta-ionone, pseudoionone, and geranylacetone","volume":"40","author":"Simkin","year":"2004","journal-title":"Plant J.: Cell Mol. 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