{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T17:45:41Z","timestamp":1793641541709,"version":"4.5.1"},"reference-count":67,"publisher":"American Chemical Society (ACS)","issue":"2","license":[{"start":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T00:00:00Z","timestamp":1769990400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T00:00:00Z","timestamp":1769990400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T00:00:00Z","timestamp":1769990400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32170093"],"award-info":[{"award-number":["32170093"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ACS Synth. Biol."],"published-print":{"date-parts":[[2026,2,20]]},"DOI":"10.1021\/acssynbio.5c00802","type":"journal-article","created":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T11:22:06Z","timestamp":1770031326000},"page":"773-788","source":"Crossref","is-referenced-by-count":3,"title":["Engineering Mixotrophy in the Chemolithoautotrophic\n                    <i>Cupriavidus necator<\/i>\n                    through Hydrogenase Induction"],"prefix":"10.1021","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7182-6008","authenticated-orcid":true,"given":"Xin","family":"Pu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Biopharmaceutical Preparation and Delivery","place":["Beijing, China"]},{"name":"Institute of Process Engineering, Chinese Academy of Sciences","place":["Beijing, China"]},{"name":"School of Chemical Engineering","place":["Beijing, China"]},{"name":"University of Chinese Academy of Sciences","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caihong","family":"Weng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Biopharmaceutical Preparation and Delivery","place":["Beijing, China"]},{"name":"Institute of Process Engineering, Chinese Academy of Sciences","place":["Beijing, China"]},{"name":"School of Chemical Engineering","place":["Beijing, China"]},{"name":"University of Chinese Academy of Sciences","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Biopharmaceutical Preparation and Delivery","place":["Beijing, China"]},{"name":"Institute of Process Engineering, Chinese Academy of Sciences","place":["Beijing, China"]},{"name":"School of Chemical Engineering","place":["Beijing, China"]},{"name":"University of Chinese Academy of Sciences","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biao","family":"Geng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Biopharmaceutical Preparation and Delivery","place":["Beijing, China"]},{"name":"Institute of Process Engineering, Chinese Academy of Sciences","place":["Beijing, China"]},{"name":"School of Chemical Engineering","place":["Beijing, China"]},{"name":"University of Chinese Academy of Sciences","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiqian","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Biopharmaceutical Preparation and Delivery","place":["Beijing, China"]},{"name":"Institute of Process Engineering, Chinese Academy of Sciences","place":["Beijing, China"]},{"name":"School of Chemical Engineering","place":["Beijing, China"]},{"name":"University of Chinese Academy of Sciences","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowei","family":"Peng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Biopharmaceutical Preparation and Delivery","place":["Beijing, China"]},{"name":"Institute of Process Engineering, Chinese Academy of Sciences","place":["Beijing, China"]},{"name":"School of Chemical Engineering","place":["Beijing, China"]},{"name":"University of Chinese Academy of Sciences","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7797-3925","authenticated-orcid":true,"given":"Yejun","family":"Han","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Biopharmaceutical Preparation and Delivery","place":["Beijing, China"]},{"name":"Institute of Process Engineering, Chinese Academy of Sciences","place":["Beijing, China"]},{"name":"School of Chemical Engineering","place":["Beijing, China"]},{"name":"University of Chinese Academy of Sciences","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,2,2]]},"reference":[{"key":"ref1\/cit1a","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2022.127806"},{"key":"ref1\/cit1b","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2020.124169"},{"key":"ref2\/cit2a","doi-asserted-by":"publisher","DOI":"10.1016\/j.cej.2021.132943"},{"key":"ref2\/cit2b","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201711302"},{"key":"ref3\/cit3a","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiosc.2021.08.004"},{"key":"ref3\/cit3b","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2021.06.010"},{"key":"ref4\/cit4a","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2020.09.002"},{"key":"ref4\/cit4b","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2020.04.009"},{"key":"ref5\/cit5","doi-asserted-by":"publisher","DOI":"10.3390\/bioengineering8110179"},{"key":"ref6\/cit6","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1244"},{"key":"ref7\/cit7a","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2025.04.001"},{"key":"ref7\/cit7b","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2023.128376"},{"key":"ref7\/cit7c","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2023.128762"},{"key":"ref7\/cit7d","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2022.946085"},{"key":"ref8\/cit8","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2021.01.001"},{"key":"ref9\/cit9","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-024-04200-x"},{"key":"ref10\/cit10","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-002-0441-3"},{"issue":"3","key":"ref11\/cit11","first-page":"311","volume":"2","author":"Grzeszik C.","year":"2000","journal-title":"J. Mol. Microbiol. Biotechnol."},{"key":"ref12\/cit12","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2017.07.005"},{"key":"ref13\/cit13","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.05702-11"},{"key":"ref14\/cit14","doi-asserted-by":"publisher","DOI":"10.1002\/cphc.200901002"},{"key":"ref15\/cit15","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.12.3197-3204.1998"},{"key":"ref16\/cit16","doi-asserted-by":"publisher","DOI":"10.1128\/aem.00748-24"},{"key":"ref17\/cit17","doi-asserted-by":"publisher","DOI":"10.1159\/000142893"},{"key":"ref18\/cit18","doi-asserted-by":"publisher","DOI":"10.1186\/s12934-020-01494-y"},{"key":"ref19\/cit19","doi-asserted-by":"publisher","DOI":"10.1128\/aem.02007-22"},{"key":"ref20\/cit20","doi-asserted-by":"publisher","DOI":"10.1128\/jb.98.2.617-622.1969"},{"key":"ref21\/cit21","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2024.102432"},{"key":"ref22\/cit22","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-022-01251-4"},{"key":"ref23\/cit23","doi-asserted-by":"publisher","DOI":"10.1093\/lambio\/ovaf061"},{"key":"ref24\/cit24","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijhydene.2016.12.021"},{"key":"ref25\/cit25","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2022.127138"},{"key":"ref26\/cit26","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2025.103298"},{"key":"ref27\/cit27","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms12800"},{"key":"ref28\/cit28","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-13-169"},{"key":"ref29\/cit29","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.69019"},{"key":"ref30\/cit30","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-020-00486-4"},{"key":"ref31\/cit31","doi-asserted-by":"publisher","DOI":"10.1186\/s12934-022-01962-7"},{"key":"ref32\/cit32","doi-asserted-by":"publisher","DOI":"10.1128\/aem.01458-21"},{"key":"ref33\/cit33","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.21.12474"},{"key":"ref34\/cit34","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2022.10.016"},{"key":"ref35\/cit35","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1600718113"},{"key":"ref36\/cit36","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-022-11842-0"},{"key":"ref37\/cit37","doi-asserted-by":"publisher","DOI":"10.3390\/fermentation8030125"},{"key":"ref38\/cit38","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-130-8-1987"},{"key":"ref39\/cit39","doi-asserted-by":"publisher","DOI":"10.1007\/s11306-017-1302-z"},{"key":"ref40\/cit40","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12507"},{"key":"ref41\/cit41","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.68.4.603-616.2004"},{"key":"ref42\/cit42","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2024.10.011"},{"key":"ref43\/cit43a","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25021277"},{"key":"ref43\/cit43b","doi-asserted-by":"publisher","DOI":"10.1038\/s41396-021-01019-4"},{"key":"ref44\/cit44","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.135"},{"key":"ref45\/cit45","doi-asserted-by":"publisher","DOI":"10.1186\/s12934-015-0226-4"},{"key":"ref46\/cit46","doi-asserted-by":"publisher","DOI":"10.3390\/catal12040399"},{"key":"ref47\/cit47","doi-asserted-by":"publisher","DOI":"10.1016\/b978-0-444-63475-7.00012-1"},{"key":"ref48\/cit48","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2025.128222"},{"key":"ref49\/cit49","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp336"},{"key":"ref50\/cit50","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3317"},{"key":"ref51\/cit51","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-12-323"},{"key":"ref52\/cit52","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/bs.mie.2018.10.008","volume-title":"Enzymes of Energy Technology","volume":"613","author":"Lenz O.","year":"2018"},{"key":"ref53\/cit53","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-013-1410-5"},{"key":"ref54\/cit54","doi-asserted-by":"publisher","DOI":"10.1186\/s13068-019-1512-x"},{"key":"ref55\/cit55a","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-021-08358-7"},{"key":"ref55\/cit55b","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.73"},{"key":"ref56\/cit56","doi-asserted-by":"publisher","DOI":"10.1002\/bit.26578"},{"key":"ref57\/cit57","doi-asserted-by":"publisher","DOI":"10.23887\/jst-undiksha.v7i1.13009"},{"key":"ref58\/cit58","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-014-5591-0"}],"container-title":["ACS Synthetic Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acssynbio.5c00802","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acssynbio.5c00802","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T08:49:50Z","timestamp":1773132590000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/doi\/10.1021\/acssynbio.5c00802"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,2]]},"references-count":67,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,2,20]]}},"alternative-id":["10.1021\/acssynbio.5c00802"],"URL":"https:\/\/doi.org\/10.1021\/acssynbio.5c00802","relation":{},"ISSN":["2161-5063","2161-5063"],"issn-type":[{"value":"2161-5063","type":"print"},{"value":"2161-5063","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,2]]}}}