{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T14:49:32Z","timestamp":1792162172428,"version":"4.4.0"},"reference-count":49,"publisher":"American Chemical Society (ACS)","issue":"10","license":[{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFA0911800"],"award-info":[{"award-number":["2022YFA0911800"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32101170"],"award-info":[{"award-number":["32101170"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>d-Pantothenic acid (D-PA) is an essential precursor of coenzyme A and the acyl carrier protein. Its biosynthesis requires abundant precursors and precise regulation of the multifunctional enzyme, ketopantoate reductase. Here, we engineered Corynebacterium glutamicum for enhanced D-PA production through metabolic engineering, spatial enzyme organization, and a dynamically controlled CPSPC system (Coenzyme and Product Synthesis Pathway Coupling System). Competing pathways were suppressed, while target synthesis routes and 5,10-methylenetetrahydrofolate supply were strengthened. A RIAD-RIDD-mediated substrate channel between dihydroxy acid dehydratase and ketopantoate hydroxymethyltransferase was used to optimize enzymatic stoichiometry and localization. After functionalizing a glycine riboswitch in C. glutamicum, we developed the CPSPC system that dynamically couples one-carbon metabolism with D-PA synthesis. Fluorescence tracking confirmed its glycine-responsive regulation. The engineered strain DPAj-2-tuf achieved 23.06 g\/L in a 5 L bioreactor over 63 h, productivity of 0.37 g\/L\/h, and glucose yield of 0.17 g\/g. These outcomes offer an efficient and scalable strategy for industrial D-PA production using C. glutamicum.<\/jats:p>","DOI":"10.1021\/acssynbio.6c00280","type":"journal-article","created":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T16:02:20Z","timestamp":1790179340000},"page":"4060-4074","source":"Crossref","is-referenced-by-count":0,"title":["Enhanced\n                    <scp>d<\/scp>\n                    -Pantothenic Acid Biosynthesis in\n                    <i>Corynebacterium glutamicum<\/i>\n                    by the CPSPC System and Spatial Organization Strategy"],"prefix":"10.1021","volume":"15","author":[{"given":"Qing","family":"Yang","sequence":"first","affiliation":[{"name":"Zhejiang University of Technology , , ,","place":["Hangzhou, China, 310014"]},{"name":"Zhejiang University of Technology , , ,","place":["Hangzhou, China, 310014"]},{"name":"Zhejiang University of Technology , , ,","place":["Hangzhou, China, 310014"]},{"name":"Zhejiang University of Technology , , ,","place":["Hangzhou, China, 310014"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Zhejiang University of Technology , , ,","place":["Hangzhou, China, 310014"]},{"name":"Zhejiang University of Technology , , ,","place":["Hangzhou, China, 310014"]},{"name":"Zhejiang University of Technology , , ,","place":["Hangzhou, China, 310014"]},{"name":"Zhejiang University of Technology , , ,","place":["Hangzhou, China, 310014"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lianggang","family":"Huang","sequence":"additional","affiliation":[{"name":"Zhejiang University of Technology , , ,","place":["Hangzhou, China, 310014"]},{"name":"Zhejiang University of Technology , , ,","place":["Hangzhou, China, 310014"]},{"name":"Zhejiang University of Technology , , ,","place":["Hangzhou, China, 310014"]},{"name":"Zhejiang University of Technology , , ,","place":["Hangzhou, China, 310014"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junping","family":"Zhou","sequence":"additional","affiliation":[{"name":"Zhejiang University of Technology , , ,","place":["Hangzhou, China, 310014"]},{"name":"Zhejiang University of Technology , , ,","place":["Hangzhou, China, 310014"]},{"name":"Zhejiang University of Technology , , ,","place":["Hangzhou, China, 310014"]},{"name":"Zhejiang University of Technology , , ,","place":["Hangzhou, China, 310014"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cuicui","family":"Liu","sequence":"additional","affiliation":[{"name":"Zhejiang University of Technology , , ,","place":["Hangzhou, China, 310014"]},{"name":"Zhejiang University of Technology , , ,","place":["Hangzhou, China, 310014"]},{"name":"Zhejiang University of Technology , , ,","place":["Hangzhou, China, 310014"]},{"name":"Zhejiang University of Technology , , ,","place":["Hangzhou, China, 310014"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3259-6796","authenticated-orcid":true,"given":"Zhiqiang","family":"Liu","sequence":"additional","affiliation":[{"name":"Zhejiang University of Technology , , ,","place":["Hangzhou, China, 310014"]},{"name":"Zhejiang University of Technology , , ,","place":["Hangzhou, China, 310014"]},{"name":"Zhejiang University of Technology , , ,","place":["Hangzhou, China, 310014"]},{"name":"Zhejiang University of Technology , , ,","place":["Hangzhou, China, 310014"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6358-6243","authenticated-orcid":true,"given":"Yuguo","family":"Zheng","sequence":"additional","affiliation":[{"name":"Zhejiang University of Technology , , ,","place":["Hangzhou, China, 310014"]},{"name":"Zhejiang University of Technology , , ,","place":["Hangzhou, China, 310014"]},{"name":"Zhejiang University of Technology , , ,","place":["Hangzhou, China, 310014"]},{"name":"Zhejiang University of Technology , , ,","place":["Hangzhou, China, 310014"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,23]]},"reference":[{"key":"2026101610065076500_cit1","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1016\/j.ymben.2025.08.003","article-title":"Heterologous integration-assisted metabolic engineering in Escherichia coli for elevated D-pantothenic acid production","volume":"92","author":"Zhao","year":"2025","journal-title":"Metab. Eng."},{"key":"2026101610065076500_cit2","doi-asserted-by":"publisher","first-page":"267","DOI":"10.1016\/j.foodchem.2019.05.044","article-title":"Metabolic engineering of Escherichia coli for D-pantothenic acid production","volume":"294","author":"Zhang","year":"2019","journal-title":"Food Chem."},{"key":"2026101610065076500_cit3","doi-asserted-by":"publisher","first-page":"158","DOI":"10.1016\/j.ymben.2024.06.006","article-title":"Development of a vitamin B5 hyperproducer in Escherichia coli by multiple metabolic engineering","volume":"84","author":"Song","year":"2024","journal-title":"Metab. Eng."},{"issue":"5","key":"2026101610065076500_cit4","doi-asserted-by":"publisher","first-page":"949","DOI":"10.1039\/b703104c","article-title":"Roles of vitamins B5, B8, B9, B12 and molybdenum cofactor at cellular and organismal levels","volume":"24","author":"Re\u0301beille\u0301","year":"2007","journal-title":"Nat. Prod. Rep."},{"issue":"3","key":"2026101610065076500_cit5","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1016\/j.bbrc.2020.05.015","article-title":"Cerebral deficiency of vitamin B5 (d-pantothenic acid; pantothenate) as a potentially-reversible cause of neurodegeneration and dementia in sporadic Alzheimer\u2019s disease","volume":"527","author":"Xu","year":"2020","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2026101610065076500_cit6","doi-asserted-by":"publisher","first-page":"169179","DOI":"10.1016\/j.cej.2025.169179","article-title":"Integrated cofactor-centric engineering and energy flux optimization enable high-efficient d-pantothenic acid production in Escherichia coli","volume":"524","author":"Wang","year":"2025","journal-title":"Chem. Eng. J."},{"issue":"8","key":"2026101610065076500_cit7","doi-asserted-by":"publisher","first-page":"1172","DOI":"10.1080\/07388551.2022.2104690","article-title":"Microbial production of vitamin B5: current status and prospects","volume":"43","author":"Zhao","year":"2022","journal-title":"Crit. Rev. Biotechnol."},{"key":"2026101610065076500_cit8","doi-asserted-by":"publisher","first-page":"1176445","DOI":"10.3389\/fbioe.2023.1176445","article-title":"System metabolic engineering of Escherichia coli W for the production of 2-ketoisovalerate using unconventional feedstock","volume":"11","author":"Carranza-Saavedra","year":"2023","journal-title":"Front. Bioeng. Biotechnol."},{"key":"2026101610065076500_cit9","doi-asserted-by":"publisher","first-page":"11248","DOI":"10.1039\/d5gc02458g","article-title":"Enhancing vitamin B5 biosynthesis by multimodule optimization and protein engineering","volume":"27","author":"Zhang","year":"2025","journal-title":"Green Chem."},{"key":"2026101610065076500_cit10","doi-asserted-by":"publisher","first-page":"142","DOI":"10.1016\/j.copbio.2014.12.022","article-title":"Engineering metabolism through dynamic control","volume":"34","author":"Venayak","year":"2015","journal-title":"Curr. Opin. Biotechnol."},{"issue":"7","key":"2026101610065076500_cit11","doi-asserted-by":"publisher","first-page":"1369","DOI":"10.1128\/jb.01480-13","article-title":"Two glycine riboswitches activate the glycine cleavage system essential for glycine detoxification in Streptomyces griseus","volume":"196","author":"Tezuka","year":"2014","journal-title":"J. Bacteriol."},{"issue":"1","key":"2026101610065076500_cit12","doi-asserted-by":"publisher","first-page":"519","DOI":"10.1146\/annurev-chembioeng-091720-125738","article-title":"Dynamic control of metabolism","volume":"12","author":"Ni","year":"2021","journal-title":"Annu. Rev. Chem. Biomol. Eng."},{"key":"2026101610065076500_cit13","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1016\/j.copbio.2017.10.009","article-title":"Production of chemicals using dynamic control of metabolic fluxes","volume":"53","author":"Xu","year":"2018","journal-title":"Curr. Opin. Biotechnol."},{"issue":"8","key":"2026101610065076500_cit14","doi-asserted-by":"publisher","first-page":"2943","DOI":"10.1007\/s00284-021-02550-5","article-title":"Regulation of glycine cleavage and detoxification by a highly conserved glycine riboswitch in Burkholderia spp","volume":"78","author":"Munyati-Othman","year":"2021","journal-title":"Curr. Microbiol."},{"issue":"12","key":"2026101610065076500_cit15","doi-asserted-by":"publisher","first-page":"1335","DOI":"10.1021\/acssynbio.5b00075","article-title":"Engineering a lysine-ON riboswitch for metabolic control of lysine production in Corynebacterium glutamicum","volume":"4","author":"Zhou","year":"2015","journal-title":"ACS Synth. Biol."},{"issue":"5","key":"2026101610065076500_cit16","doi-asserted-by":"publisher","first-page":"774","DOI":"10.1016\/j.molcel.2010.11.026","article-title":"Structural insights into ligand recognition by a sensing domain of the cooperative glycine riboswitch","volume":"40","author":"Huang","year":"2010","journal-title":"Mol. Cell"},{"issue":"12","key":"2026101610065076500_cit17","doi-asserted-by":"publisher","first-page":"5983","DOI":"10.1093\/nar\/gkw465","article-title":"Engineering and characterization of fluorogenic glycine riboswitches","volume":"44","author":"Ketterer","year":"2016","journal-title":"Nucleic Acids Res."},{"issue":"10","key":"2026101610065076500_cit18","doi-asserted-by":"publisher","first-page":"2327","DOI":"10.1021\/acssynbio.9b00137","article-title":"Characterization and engineering of a clostridium glycine riboswitch and its use to control a novel metabolic pathway for 5-aminolevulinic acid production in Escherichia coli","volume":"8","author":"Zhou","year":"2019","journal-title":"ACS Synth. Biol."},{"issue":"1","key":"2026101610065076500_cit19","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1186\/s12934-022-01779-4","article-title":"Development and characterization of a glycine biosensor system for fine-tuned metabolic regulation in Escherichia coli","volume":"21","author":"Hong","year":"2022","journal-title":"Microb. Cell Fact."},{"issue":"9","key":"2026101610065076500_cit20","doi-asserted-by":"publisher","first-page":"2378","DOI":"10.1021\/acssynbio.0c00127","article-title":"Dynamic control of 4-hydroxyisoleucinebiosynthesis by modified l-isoleucine biosensor in recombinant Corynebacterium glutamicum","volume":"9","author":"Tan","year":"2020","journal-title":"ACS Synth. Biol."},{"issue":"3","key":"2026101610065076500_cit21","doi-asserted-by":"publisher","first-page":"865","DOI":"10.1128\/aem.07056-11","article-title":"Improvement of the redox balance increases l-valine production by Corynebacterium glutamicum under oxygen deprivation conditions","volume":"78","author":"Hasegawa","year":"2012","journal-title":"Appl. Environ. Microbiol."},{"issue":"9","key":"2026101610065076500_cit22","doi-asserted-by":"publisher","first-page":"2861","DOI":"10.1021\/acssynbio.4c00278","article-title":"Metabolic engineering of Corynebacterium glutamicum for the high-level production of l-valine under aerobic conditions","volume":"13","author":"Wang","year":"2024","journal-title":"ACS Synth. Biol."},{"issue":"2","key":"2026101610065076500_cit23","doi-asserted-by":"publisher","first-page":"635","DOI":"10.1021\/acssynbio.7b00373","article-title":"Reprogramming one-carbon metabolic pathways to decouple l-serine catabolism from cell growth in Corynebacterium glutamicum","volume":"7","author":"Zhang","year":"2018","journal-title":"ACS Synth. Biol."},{"issue":"11","key":"2026101610065076500_cit24","doi-asserted-by":"publisher","first-page":"1916","DOI":"10.4014\/jmb.1805.05013","article-title":"Comparative genomic and genetic functional analysis of industrial l-leucine and l-valine producing Corynebacterium glutamicum strains","volume":"28","author":"Ma","year":"2018","journal-title":"J. Microbiol. Biotechnol."},{"issue":"1","key":"2026101610065076500_cit25","doi-asserted-by":"publisher","first-page":"3632","DOI":"10.1038\/s41598-018-21926-5","article-title":"Understanding the high l-valine production in Corynebacterium glutamicum VWB-1 using transcriptomics and proteomics","volume":"8","author":"Zhang","year":"2018","journal-title":"Sci. Rep."},{"key":"2026101610065076500_cit26","doi-asserted-by":"publisher","first-page":"127024","DOI":"10.1016\/j.biortech.2022.127024","article-title":"Module engineering coupled with omics strategies for enhancing D-pantothenate production in Escherichia coli","volume":"352","author":"Wang","year":"2022","journal-title":"Bioresour. Technol."},{"issue":"18","key":"2026101610065076500_cit27","doi-asserted-by":"publisher","first-page":"5566","DOI":"10.1128\/aem.01741-13","article-title":"Platform engineering of Corynebacterium glutamicum with reduced pyruvate dehydrogenase complex activity for improved production of l-lysine, l-valine, and 2-ketoisovalerate","volume":"79","author":"Buchholz","year":"2013","journal-title":"Appl. Environ. Microbiol."},{"issue":"6","key":"2026101610065076500_cit28","doi-asserted-by":"publisher","first-page":"3255","DOI":"10.1128\/aem.71.6.3255-3268.2005","article-title":"Rational design of a Corynebacterium glutamicum pantothenate production strain and its characterization by metabolic flux analysis and genome-wide transcriptional profiling","volume":"71","author":"Hu\u0308ser","year":"2005","journal-title":"Appl. Environ. Microbiol."},{"key":"2026101610065076500_cit29","doi-asserted-by":"publisher","first-page":"110330","DOI":"10.1016\/j.enzmictec.2023.110330","article-title":"2-Hydroxyisovalerate production by Klebsiella pneumoniae","volume":"172","author":"Wang","year":"2024","journal-title":"Enzyme Microb. Technol."},{"key":"2026101610065076500_cit30","doi-asserted-by":"publisher","first-page":"131325","DOI":"10.1016\/j.biortech.2024.131325","article-title":"Efficient carbon flux allocation towards D-pantothenic acid production via growth-decoupled strategy in Escherichia coli","volume":"411","author":"Wang","year":"2024","journal-title":"Bioresour. Technol."},{"issue":"2","key":"2026101610065076500_cit31","doi-asserted-by":"publisher","first-page":"383","DOI":"10.1007\/s10529-017-2479-y","article-title":"Native promoters of Corynebacterium glutamicum and its application in l-lysine production","volume":"40","author":"Shang","year":"2017","journal-title":"Biotechnol. Lett."},{"issue":"5","key":"2026101610065076500_cit32","doi-asserted-by":"publisher","first-page":"1759","DOI":"10.1007\/s00253-021-11121-4","article-title":"Research progress and the biotechnological applications of multienzyme complex","volume":"105","author":"Jiang","year":"2021","journal-title":"Appl. Microbiol. Biotechnol."},{"issue":"1","key":"2026101610065076500_cit33","doi-asserted-by":"publisher","first-page":"4248","DOI":"10.1038\/s41467-019-12247-w","article-title":"Modular enzyme assembly for enhanced cascade biocatalysis and metabolic flux","volume":"10","author":"Kang","year":"2019","journal-title":"Nat. Commun."},{"issue":"12","key":"2026101610065076500_cit34","doi-asserted-by":"publisher","first-page":"751","DOI":"10.1038\/s44286-024-00148-9","article-title":"A bacterial platform for producing aromatic esters from glycerol","volume":"1","author":"Lu","year":"2024","journal-title":"Nat. Chem. Eng."},{"issue":"4","key":"2026101610065076500_cit35","doi-asserted-by":"publisher","first-page":"437","DOI":"10.1007\/s00253-002-1161-y","article-title":"3-Phosphoglycerate dehydrogenase from Corynebacterium glutamicum: the C-terminal domain is not essential for activity but is required for inhibition by l-serine","volume":"60","author":"Peters","year":"2002","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"2026101610065076500_cit36","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1016\/j.ymben.2015.10.013","article-title":"Metabolic engineering of Corynebacterium glutamicum for the de novo production of ethylene glycol from glucose","volume":"33","author":"Chen","year":"2016","journal-title":"Metab. Eng."},{"issue":"4","key":"2026101610065076500_cit37","doi-asserted-by":"publisher","first-page":"717","DOI":"10.1128\/jb.02365-14","article-title":"One-carbon metabolic pathway rewiring in Escherichia coli reveals an evolutionary advantage of 10-formyltetrahydrofolatesynthetase (Fhs) in survival under hypoxia","volume":"197","author":"Sah","year":"2015","journal-title":"J. Bacteriol."},{"issue":"1","key":"2026101610065076500_cit38","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1186\/s13068-019-1410-2","article-title":"Modular systems metabolic engineering enables balancing of relevant pathways for l-histidine production with Corynebacterium glutamicum","volume":"12","author":"Schwentner","year":"2019","journal-title":"Biotechnol. Biofuels"},{"issue":"5694","key":"2026101610065076500_cit39","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1126\/science.1100829","article-title":"A glycine-dependent riboswitch that uses cooperative binding to control gene expression","volume":"306","author":"Mandal","year":"2004","journal-title":"Science"},{"key":"2026101610065076500_cit40","doi-asserted-by":"publisher","first-page":"130774","DOI":"10.1016\/j.biortech.2024.130774","article-title":"Systems metabolic engineering of Corynebacterium glutamicum to assimilate formic acid for biomass accumulation and succinic acid production","volume":"402","author":"Li","year":"2024","journal-title":"Bioresour. Technol."},{"issue":"7","key":"2026101610065076500_cit41","doi-asserted-by":"publisher","first-page":"1426","DOI":"10.1261\/rna.1623809","article-title":"Computational identification of riboswitches based on RNA conserved functional sequences and conformations","volume":"15","author":"Chang","year":"2009","journal-title":"RNA"},{"issue":"3","key":"2026101610065076500_cit42","doi-asserted-by":"publisher","first-page":"486","DOI":"10.1016\/j.jbiotec.2006.12.007","article-title":"Development of a glycine-inducible expression system for Bacillus subtilis","volume":"128","author":"Phan","year":"2007","journal-title":"J. Biotechnol."},{"issue":"5","key":"2026101610065076500_cit43","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1038\/nmeth.1318","article-title":"Enzymatic assembly of DNA molecules up to several hundred kilobases","volume":"6","author":"Gibson","year":"2009","journal-title":"Nat. Methods"},{"key":"2026101610065076500_cit44","doi-asserted-by":"publisher","first-page":"681","DOI":"10.1016\/j.procbio.2022.08.012","article-title":"Combing with redox regulation via quorum-sensing system and fermentation strategies for improving D-pantothenic acid production","volume":"121","author":"Qin","year":"2022","journal-title":"Process Biochem"},{"issue":"10","key":"2026101610065076500_cit45","doi-asserted-by":"publisher","first-page":"2983","DOI":"10.1021\/acssynbio.3c00315","article-title":"Dynamically regulating glucose uptake to reduce overflow metabolism with a quorum-sensing circuit for the efficient synthesis of D-pantothenic acid in Bacillus subtilis","volume":"12","author":"Yuan","year":"2023","journal-title":"ACS Synth. Biol."},{"key":"2026101610065076500_cit46","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1016\/j.ymben.2021.01.005","article-title":"Dynamic control over feedback regulatory mechanisms improves NADPH flux and xylitol biosynthesis in engineered E. coli","volume":"64","author":"Li","year":"2021","journal-title":"Metab. Eng."},{"issue":"3","key":"2026101610065076500_cit47","doi-asserted-by":"publisher","first-page":"346","DOI":"10.1038\/s41589-022-01172-7","article-title":"Probing coenzyme A homeostasis with semisynthetic biosensors","volume":"19","author":"Xue","year":"2023","journal-title":"Nat. Chem. Biol."},{"issue":"1","key":"2026101610065076500_cit48","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1016\/0378-1119(94)90324-7","article-title":"Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum","volume":"145","author":"Scha\u0308fer","year":"1994","journal-title":"Gene"},{"key":"2026101610065076500_cit49","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1016\/j.ymben.2022.08.012","article-title":"Metabolic engineering of E. coli for \u03b2-alanine production using a multi-biosensor enabled approach","volume":"74","author":"Yuan","year":"2022","journal-title":"Metab. Eng."}],"container-title":["ACS Synthetic Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acssynbio.6c00280","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/asbcd6\/article-pdf\/15\/10\/4060\/68623299\/sb6c00280.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/asbcd6\/article-pdf\/15\/10\/4060\/68623299\/sb6c00280.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T14:23:32Z","timestamp":1792160612000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/asbcd6\/article\/15\/10\/4060\/5436751\/Enhanced-d-Pantothenic-Acid-Biosynthesis-in"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,23]]},"references-count":49,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2026,9,23]]},"published-print":{"date-parts":[[2026,10,16]]}},"URL":"https:\/\/doi.org\/10.1021\/acssynbio.6c00280","relation":{},"ISSN":["2161-5063"],"issn-type":[{"value":"2161-5063","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,23]]}}}