{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T14:49:39Z","timestamp":1792162179423,"version":"4.4.0"},"reference-count":24,"publisher":"American Chemical Society (ACS)","issue":"10","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32270100"],"award-info":[{"award-number":["32270100"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32271481"],"award-info":[{"award-number":["32271481"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32671900"],"award-info":[{"award-number":["32671900"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002367","name":"Chinese Academy of Sciences","doi-asserted-by":"publisher","award":["XDC0110300"],"award-info":[{"award-number":["XDC0110300"]}],"id":[{"id":"10.13039\/501100002367","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project","award":["25ZXWCSY00250"],"award-info":[{"award-number":["25ZXWCSY00250"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>l-Glutamic acid is conventionally produced by bacteria from starch-derived sugar, while its production in eukaryotic hosts remains rarely reported. The thermophilic ascomycete fungus Myceliophthora thermophila has been developed as a platform for producing value-added chemicals from lignocellulosic biomass. In this study, we explored the feasibility of engineering M. thermophila for l-glutamate production from biomass-derived carbon sources. Genome-scale metabolic modeling indicated that M. thermophila has the stoichiometric capacity to synthesize l-glutamate from glucose and ammonia. Experimental fermentation confirmed extracellular l-glutamate accumulation, and medium optimization revealed that nitrogen source selection and extracellular pH homeostasis strongly affected l-glutamate accumulation. Overexpression of the native NADPH-dependent glutamate dehydrogenase gene gdh1 markedly increased glutamate production. Additional engineering strategies targeting membrane-associated metabolism, glutamate transport, mitochondrial precursor transport, and competing \u03b1-ketoglutarate-consuming pathways provided insights into the regulation of glutamate metabolism in filamentous fungi. The engineered strain produced 3.41 g\/L l-glutamate from glucose. Moreover, 1.50 g\/L and 1.49 g\/L l-glutamate were obtained directly from cellulose and raw corncob, respectively. This study establishes M. thermophila as a proof-of-concept thermophilic fungal platform for l-glutamate production from lignocellulosic carbon sources and provides a framework for engineering amino acid biosynthesis in cellulolytic fungi.<\/jats:p>","DOI":"10.1021\/acssynbio.6c00533","type":"journal-article","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T15:07:06Z","timestamp":1790694426000},"page":"4327-4336","source":"Crossref","is-referenced-by-count":0,"title":["Metabolic Engineering of the Thermophilic Fungus\n                    <i>Myceliophthora thermophila<\/i>\n                    for\n                    <scp>l<\/scp>\n                    -glutamate Production from Lignocellulosic Biomass"],"prefix":"10.1021","volume":"15","author":[{"given":"Defei","family":"Liu","sequence":"first","affiliation":[{"name":"Chinese Academy of Sciences , , ,","place":["Tianjin, China, 300308"]},{"name":"National Technology Innovation Center for Synthetic Biology , ,","place":["Tianjin, China, 300308"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yutong","family":"Ge","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences , , ,","place":["Tianjin, China, 300308"]},{"name":"National Technology Innovation Center for Synthetic Biology , ,","place":["Tianjin, China, 300308"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7018-3258","authenticated-orcid":true,"given":"Jingen","family":"Li","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences , , ,","place":["Tianjin, China, 300308"]},{"name":"National Technology Innovation Center for Synthetic Biology , ,","place":["Tianjin, China, 300308"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaoguang","family":"Tian","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences , , ,","place":["Tianjin, China, 300308"]},{"name":"National Technology Innovation Center for Synthetic Biology , ,","place":["Tianjin, China, 300308"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,29]]},"reference":[{"key":"2026101610071729000_cit1","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1016\/j.ymben.2019.03.008","article-title":"Metabolic engineering advances and prospects for amino acid production","volume":"58","author":"Wendisch","year":"2020","journal-title":"Metab. 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