{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T19:53:43Z","timestamp":1760644423271},"reference-count":55,"publisher":"American Chemical Society (ACS)","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioconjugate Chem."],"published-print":{"date-parts":[[2000,11,1]]},"DOI":"10.1021\/bc0000379","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T05:51:32Z","timestamp":1027662692000},"page":"842-854","source":"Crossref","is-referenced-by-count":44,"title":["Experimental Pretargeting Studies of Cancer with a Humanized anti-CEA \u00d7 Murine anti-[In-DTPA] Bispecific Antibody Construct and a <sup>99m<\/sup>Tc-\/<sup>188<\/sup>Re-Labeled Peptide"],"prefix":"10.1021","volume":"11","author":[{"given":"H.","family":"Karacay","sequence":"first","affiliation":[{"name":"Immunomedics, Inc., Morris Plains, New Jersey, Garden State Cancer Center, Belleville, New Jersey, and IBC Pharmaceuticals, LLC, Morris Plains, New Jersey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. J.","family":"McBride","sequence":"additional","affiliation":[{"name":"Immunomedics, Inc., Morris Plains, New Jersey, Garden State Cancer Center, Belleville, New Jersey, and IBC Pharmaceuticals, LLC, Morris Plains, New Jersey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. L.","family":"Griffiths","sequence":"additional","affiliation":[{"name":"Immunomedics, Inc., Morris Plains, New Jersey, Garden State Cancer Center, Belleville, New Jersey, and IBC Pharmaceuticals, LLC, Morris Plains, New Jersey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. M.","family":"Sharkey","sequence":"additional","affiliation":[{"name":"Immunomedics, Inc., Morris Plains, New Jersey, Garden State Cancer Center, Belleville, New Jersey, and IBC Pharmaceuticals, LLC, Morris Plains, New Jersey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Barbet","sequence":"additional","affiliation":[{"name":"Immunomedics, Inc., Morris Plains, New Jersey, Garden State Cancer Center, Belleville, New Jersey, and IBC Pharmaceuticals, LLC, Morris Plains, New Jersey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. J.","family":"Hansen","sequence":"additional","affiliation":[{"name":"Immunomedics, Inc., Morris Plains, New Jersey, Garden State Cancer Center, Belleville, New Jersey, and IBC Pharmaceuticals, LLC, Morris Plains, New Jersey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. M.","family":"Goldenberg","sequence":"additional","affiliation":[{"name":"Immunomedics, Inc., Morris Plains, New Jersey, Garden State Cancer Center, Belleville, New Jersey, and IBC Pharmaceuticals, LLC, Morris Plains, New Jersey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"316","published-online":{"date-parts":[[2000,11,3]]},"reference":[{"key":"bc0000379b00001\/bc0000379b00001_1","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1097\/00002371-199408000-00049","volume":"16","author":"Axworthy D. B.","year":"1994","journal-title":"J. Immunother."},{"key":"bc0000379b00002\/bc0000379b00002_1","volume-title":"Immunoconjugates, Radiopharm. 5, 385\u2212395.","author":"Bamias A.","year":"1992"},{"key":"bc0000379b00003\/bc0000379b00003_1","volume":"39","author":"Barbet J.","year":"1998","journal-title":"J. Nucl. Med."},{"key":"bc0000379b00004\/bc0000379b00004_1","volume-title":"Reduction in the renal uptake of radiolabeled monoclonal antibody fragments by cationic amino acids and their derivatives. Cancer Res. 55, 3825\u22123834","author":"Behr T. M.","year":"1995"},{"key":"bc0000379b00005\/bc0000379b00005_1","volume-title":"Pretargeting of renal cell carcinoma:\u2009 improved tumor targeting with a bivalent chelate. Cancer Res. 59, 4400\u22124405","author":"Boerman O. C.","year":"1999"},{"key":"bc0000379b00006\/bc0000379b00006_1","volume-title":"vitro evaluation of DNA-DNA hybridization as a two-step approach in radioimmunotherapy of cancer. Cancer Res. 54, 3479\u22123486","author":"Bos E. S.","year":"1994"},{"key":"bc0000379b00007\/bc0000379b00007_1","volume-title":"Engineering antibodies for imaging and therapy. Curr. Opin. Biotechnol. 8, 449\u2212454","author":"Carter P.","year":"1997"},{"key":"bc0000379b00008\/bc0000379b00008_1","doi-asserted-by":"crossref","DOI":"10.1007\/BF00812361","volume":"17","author":"Chetanneau A.","year":"1990","journal-title":"Eur. J. Nucl. Med."},{"key":"bc0000379b00009\/bc0000379b00009_1","doi-asserted-by":"crossref","DOI":"10.1038\/bjc.1998.463","volume":"78","author":"Chinol M.","year":"1998","journal-title":"Br. J. Cancer"},{"key":"bc0000379b00010\/bc0000379b00010_1","volume-title":"Design and production of novel tetravalent bispecific antibodies. Nat. Biotechnol. 15, 159\u2212163","author":"Coloma M. J.","year":"1997"},{"key":"bc0000379b00011\/bc0000379b00011_1","volume":"26","author":"Cremonesi M.","year":"1999","journal-title":"Eur. J. Nucl. Med."},{"key":"bc0000379b00012\/bc0000379b00012_1","doi-asserted-by":"crossref","DOI":"10.1021\/jo00231a027","volume":"52","author":"Dangles O.","year":"1987","journal-title":"J. Org. Chem."},{"key":"bc0000379b00013\/bc0000379b00013_1","first-page":"21","volume":"18","author":"DeNardo S. J.","year":"1999","journal-title":"Hybridoma"},{"key":"bc0000379b00014\/bc0000379b00014_1","volume-title":"New anti-Cu-TETA and anti-Y-DOTA monoclonal antibodies for potential use in the pretargeted delivery of radiopharmaceuticals to tumors. Hybridoma 17, 125\u2212132","author":"Feng X.","year":"1998"},{"key":"bc0000379b00015\/bc0000379b00015_1","volume-title":"Therapy for colon carcinoma xenografts with bispecific antibody-targeted, iodine-131-labeled bivalent hapten. Cancer 80 (Suppl.), 2618\u22122623","author":"Gautherot E.","year":"1997"},{"key":"bc0000379b00016\/bc0000379b00016_1","volume":"39","author":"Gautherot E.","year":"1998","journal-title":"J. Nucl. Med."},{"key":"bc0000379b00017\/bc0000379b00017_1","volume":"41","author":"Gautherot E.","year":"2000","journal-title":"J. Nucl. Med."},{"key":"bc0000379b00018\/bc0000379b00018_1","volume-title":"Preparation and performance of bispecific F(ab\u2018\u03b3)2 antibody containing thioether-linked Fab\u2018\u03b3 fragments. J. Immunol. 139, 2367\u22122375","author":"Glennie M. J.","year":"1987"},{"key":"bc0000379b00019\/bc0000379b00019_1","volume-title":"GW-39:\u2009 A new human tumor serially transplantable in the golden hamster. Transplantation 4, 760\u2212763","author":"Goldenberg D. M.","year":"1966"},{"key":"bc0000379b00020\/bc0000379b00020_1","volume-title":"Carcinoembryonic antigen present in human colonic neopasms serially propagated in hamsters. Science 175, 1117\u22121118","author":"Goldenberg D. M.","year":"1972"},{"key":"bc0000379b00021\/bc0000379b00021_1","volume":"29","author":"Goodwin D. A.","year":"1988","journal-title":"J. Nucl. Med."},{"key":"bc0000379b00022\/bc0000379b00022_1","doi-asserted-by":"crossref","DOI":"10.1042\/bj0890114","volume":"89","author":"Greenwood F. C.","year":"1963","journal-title":"Biochem. J."},{"key":"bc0000379b00023\/bc0000379b00023_1","volume-title":"Direct radiolabeling of monoclonal antibodies with generator-produced rhenium-188 for radioimmunotherapy:\u2009 labeling and animal biodistribution studies. Cancer Res. 51, 4594\u22124602","author":"Griffiths G. L.","year":"1991"},{"key":"bc0000379b00024\/bc0000379b00024_1","volume-title":"Preclinical evaluation of an \u201cinstant","author":"Hansen H. J.","year":"1990"},{"key":"bc0000379b00025\/bc0000379b00025_1","doi-asserted-by":"crossref","DOI":"10.1016\/0883-2897(91)90107-V","volume":"18","author":"Harrison A.","year":"1991","journal-title":"Int. J. Rad. Appl. Instrum. B"},{"key":"bc0000379b00026\/bc0000379b00026_1","volume-title":"Delivery of radionuclides to pretargeted monoclonal antibodies using dihydrofolate reductase and methotrexate in an affinity system. Cancer Res. 53, 2368\u22122373","author":"Hawkins G. A.","year":"1993"},{"key":"bc0000379b00027\/bc0000379b00027_1","volume":"28","author":"Hnatowich D. J.","year":"1987","journal-title":"J. Nucl. Med."},{"key":"bc0000379b00028\/bc0000379b00028_1","volume-title":"Color test for detection of free terminal amino groups in the solid-phase synthesis of peptides. Anal. Biochem. 34, 595\u2212598","author":"Kaiser E.","year":"1970"},{"key":"bc0000379b00029\/bc0000379b00029_1","doi-asserted-by":"crossref","unstructured":"Karacay, H. Sharkey, R. M., Griffiths, G. L., Govindan, S. V., McBride, W. J., Goldenberg, D. M., and Hansen, H. J. (1997) Development of a streptavidin-anti-carcinoembryonic antigen antibody, radiolabeled biotin pretargeting method for radioimmunotherapy of colorectal cancer:\u2009 Reagent development.Bioconjugate Chem. 8,585\u2212594.","DOI":"10.1021\/bc970102n"},{"key":"bc0000379b00030\/bc0000379b00030_1","volume-title":"Processing of reactor-produced tungsten-188 for fabrication of clinical scale alumina-based tungsten-188\/rhenium-188 generators. Appl. Radiat. Isot. 45, 1123\u22121128","author":"Knapp F. F., Jr.","year":"1994"},{"key":"bc0000379b00031\/bc0000379b00031_1","volume":"30","author":"LeDoussal J. M.","year":"1989","journal-title":"J. Nucl. Med."},{"key":"bc0000379b00032\/bc0000379b00032_1","volume-title":"Targeting of indium-111-labeled bivalent hapten to human melanoma mediated by bispecific monoclonal antibody conjugates:\u2009 Imaging of tumors hosted in nude mice. Cancer Res. 50, 3445\u22123452","author":"Le Doussal J.-M.","year":"1990"},{"key":"bc0000379b00033\/bc0000379b00033_1","volume":"34","author":"LeDoussal J.-M.","year":"1993","journal-title":"J. Nucl. Med."},{"key":"bc0000379b00034\/bc0000379b00034_1","volume-title":"B-cell (CD22)-specific, leukemia\/lymphoma antibody, LL2. Mol. Immunol. 32, 1413\u22121427.","author":"Leung S. O.","year":"1995"},{"key":"bc0000379b00035\/bc0000379b00035_1","unstructured":"Leung, S.O., Qu, Z., Hansen, H. J., Shih, L. B., Wang, J., Losman, M. J., Goldenberg, D. M., and Sharkey, R. M. (1999) The effects of domain deletion, glycosylation, and long IgG3hinge on the biodistribution and serum stability properties of a humanized IgG1immunoglobulin, hLL2, and its fragments.Clin. Cancer Res. Suppl. 5, 3106s\u22123117s."},{"key":"bc0000379b00036\/bc0000379b00036_1","doi-asserted-by":"crossref","DOI":"10.1002\/ijc.2910560419","volume":"56","author":"Losman M. J.","year":"1994","journal-title":"Int. J. Cancer"},{"key":"bc0000379b00037\/bc0000379b00037_1","unstructured":"Losman, M. J., Qu, Z., Krishnan, I. S., Wang, J., Hansen, H. J., Goldenberg, D. M., and Leung, S.O. (1999) Generation and monitoring of cell lines producing humanized antibodies.Clin. Cancer Res. Suppl. 5, 3101s\u22123105s."},{"key":"bc0000379b00038\/bc0000379b00038_1","volume-title":"Conjugation of antibodies with bifunctional chelating agents:\u2009 Isothiocyanate and bromoacetamide reagents, methods of analysis, and subsequent addition of metal ions. Anal. Biochem. 142, 68\u221278","author":"Meares C. F.","year":"1984"},{"key":"bc0000379b00039\/bc0000379b00039_1","volume":"2","author":"Paganelli G.","year":"1988","journal-title":"Int. J. Cancer Suppl."},{"key":"bc0000379b00040\/bc0000379b00040_1","volume-title":"A centrifuged column procedure for the measurement of ligand binding by beef heart F1. Part G. Methods Enzymol. 56, 527\u2212530","author":"Penefsky H. S.","year":"1979"},{"key":"bc0000379b00041\/bc0000379b00041_1","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1007\/s002620050456","volume":"46","author":"Reddy N.","year":"1998","journal-title":"Cancer Immunol. Immunother."},{"key":"bc0000379b00042\/bc0000379b00042_1","volume-title":"Pharmacokinetics and biodistribution of radiolabeled avidin, streptavidin and biotin. Nucl. Med. Biol. 20, 663\u2212668","author":"Rosebrough S. F.","year":"1993"},{"key":"bc0000379b00043\/bc0000379b00043_1","volume":"37","author":"Rusckowski M.","year":"1996","journal-title":"J. Nucl. Med."},{"key":"bc0000379b00044\/bc0000379b00044_1","volume-title":"Multistep tumor targeting in nude mice using bispecific antibodies and a gallium chelate suitable for immunoscintigraphy with positron emission tomography. Cancer Res. 55, 115\u2212123","author":"Schumacher J.","year":"1995"},{"key":"bc0000379b00045\/bc0000379b00045_1","volume-title":"Biodistribution and radiation dose estimates for yttrium- and iodine-labeled monoclonal antibody IgG and fragments in nude mice bearing human colonic tumor xenografts. Cancer Res. 50, 2330\u22122336","author":"Sharkey R. M.","year":"1990"},{"key":"bc0000379b00046\/bc0000379b00046_1","volume-title":"Rapid blood clearance of IgG2a and IgG2b in nude mice. Cancer Res. 51, 3102\u22123107","author":"Sharkey R. M.","year":"1991"},{"key":"bc0000379b00047\/bc0000379b00047_1","volume-title":"Evaluation of a complementarity-determining region-grafted (humanized) anti-carcinoembryonic antigen monoclonal antibody in preclinical and clinical studies. Cancer Res. 55, 5935s\u22125945s","author":"Sharkey R. M.","year":"1995"},{"key":"bc0000379b00048\/bc0000379b00048_1","doi-asserted-by":"crossref","unstructured":"Sharkey, R. M., Karacay, H., Griffiths, G. L., Behr, T. M., Blumenthal, R. D., Mattes, M. J., Hansen, H. J., and Goldenberg, D. M. (1997) Development of a streptavidin-anti-carcinoembryonic antigen antibody, radiolabeled biotin pretargeting method for radioimmunotherapy of colorectal cancer. Studies in a human colon cancer xenograft model.Bioconjugate Chem. 8, 595\u2212604.","DOI":"10.1021\/bc970101v"},{"key":"bc0000379b00049\/bc0000379b00049_1","volume-title":"Tumor targeting and pharmacokinetics of unmodified and modified F(ab)2 fragments of an anti-CEA murine monoclonal antibody (Immu-14). Nucl. Med. Biol. 22, 425\u2212435","author":"Siddiqui A.","year":"1995"},{"key":"bc0000379b00050\/bc0000379b00050_1","first-page":"803","volume":"29","author":"Siegel J. A.","year":"1988","journal-title":"J. Nucl. Med."},{"key":"bc0000379b00051\/bc0000379b00051_1","volume-title":"Bifunctional antibody:\u2009 A binary radiopharmaceutical delivery system for imaging colorectal carcinoma. Cancer Res. 51, 6650\u22126655","author":"Stickney D. R.","year":"1991"},{"key":"bc0000379b00052\/bc0000379b00052_1","volume":"38","author":"Vuillez J. P.","year":"1997","journal-title":"J. Nucl. Med."},{"key":"bc0000379b00053\/bc0000379b00053_1","unstructured":"Vuillez, J. P., Kraeber-Bodere, F., Moro, D., Bardies, M., Douillard, J. Y., Gautherot, E., Rouvier, E., Barbet, J., Garban, F., Moreau, P., and Chatal, J. F. (1999) Radioimmunotherapy of small cell lung carcinoma with the two-step method using a bispecific anti-carcinoembryonic antigen\/anti-diethylenetriaminepentaacetic acid (DTPA) antibody and iodine-131 Di-DTPA hapten:\u2009 results of a Phase I\/II trial.Clin. Cancer Res.5, 3259s\u22123267s."},{"key":"bc0000379b00054\/bc0000379b00054_1","doi-asserted-by":"crossref","unstructured":"Wilbur, D. S., Stayton, P. S., To, R., Buhler, K. R., Klumb, L. A., Hamlin, D. K., Stray, J. E., and Vessella, R. L. (1998) Streptavidin in antibody pretargeting. Comparison of a recombinant streptavidin with two streptavidin mutant proteins and two commercially available streptavidin proteins.Bioconjugate Chem.9, 100\u2212107.","DOI":"10.1021\/bc970152s"},{"key":"bc0000379n00001\/bc0000379n00001_1","volume-title":"bispecific antibody","author":"Abbreviations"}],"container-title":["Bioconjugate Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/bc0000379","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,3,14]],"date-time":"2023-03-14T11:03:17Z","timestamp":1678791797000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/doi\/10.1021\/bc0000379"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,11,1]]},"references-count":55,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2000,11,1]]}},"alternative-id":["10.1021\/bc0000379"],"URL":"https:\/\/doi.org\/10.1021\/bc0000379","relation":{},"ISSN":["1043-1802","1520-4812"],"issn-type":[{"value":"1043-1802","type":"print"},{"value":"1520-4812","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,11,1]]}}}