{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T22:22:44Z","timestamp":1785363764612,"version":"3.55.0"},"reference-count":41,"publisher":"American Chemical Society (ACS)","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioconjugate Chem."],"published-print":{"date-parts":[[2008,7,1]]},"DOI":"10.1021\/bc7003878","type":"journal-article","created":{"date-parts":[[2008,6,24]],"date-time":"2008-06-24T07:01:25Z","timestamp":1214290885000},"page":"1352-1360","source":"Crossref","is-referenced-by-count":39,"title":["PEGylation of Human Serum Albumin: Reaction of PEG-Phenyl-Isothiocyanate with Protein"],"prefix":"10.1021","volume":"19","author":[{"given":"Fantao","family":"Meng","sequence":"first","affiliation":[{"name":"Department of Physiology and Biophysics and Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461, and BioAffinity Systems, Rockford, Illinois 61109"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Belur N.","family":"Manjula","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics and Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461, and BioAffinity Systems, Rockford, Illinois 61109"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul K.","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics and Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461, and BioAffinity Systems, Rockford, Illinois 61109"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seetharama A.","family":"Acharya","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics and Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461, and BioAffinity Systems, Rockford, Illinois 61109"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2008,6,24]]},"reference":[{"key":"ref1\/cit1","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1038\/nrd1033","volume":"2","author":"Harris J. M.","year":"2003","journal-title":"Nat. Rev. Drug Discovery"},{"key":"ref2\/cit2","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/S0014-827X(99)00066-X","volume":"54","author":"Veronese F. M.","year":"1999","journal-title":"I1 Farmaco"},{"key":"ref3\/cit3","doi-asserted-by":"crossref","first-page":"3578","DOI":"10.1016\/S0021-9258(17)40291-2","volume":"252","author":"Abuchowski A.","year":"1977","journal-title":"J. Biol. Chem."},{"key":"ref4\/cit4","first-page":"3583","volume":"252","author":"Abuchowski A.","year":"1977","journal-title":"J. Biol. Chem."},{"key":"ref5\/cit5","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1007\/12_022","volume":"192","author":"Pasut G.","year":"2006","journal-title":"Adv. Polym. Sci."},{"key":"ref6\/cit6","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1080\/10717540600814402","volume":"13","author":"Hamidi M.","year":"2006","journal-title":"Drug Delivery"},{"key":"ref7\/cit7","doi-asserted-by":"crossref","first-page":"261","DOI":"10.2174\/138920106777950852","volume":"7","author":"Kumar S.","year":"2006","journal-title":"Curr. Pharm. Biotechnol."},{"key":"ref8\/cit8","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/0169-409X(95)00023-Z","volume":"16","author":"Zalipsky S.","year":"1995","journal-title":"Adv. Drug Delivery Rev."},{"key":"ref9\/cit9","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1016\/S0169-409X(02)00022-4","volume":"54","author":"Roberts M. J.","year":"2002","journal-title":"Adv. Drug Delivery Rev."},{"key":"ref10\/cit10","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1021\/bc960059o","volume":"7","author":"Greenwald R. B.","year":"1996","journal-title":"Bioconjugate Chem."},{"key":"ref12\/cit12","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1081\/BIO-200066365","volume":"33","author":"Acharya S. A.","year":"2005","journal-title":"Artif\/ Cells Blood Substit. Immobil. Biotechnol."},{"key":"ref13\/cit13","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1111\/j.1537-2995.2005.04290.x","volume":"45","author":"Nacharaju P.","year":"2005","journal-title":"Transfusion"},{"key":"ref14\/cit14","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1021\/bc0200733","volume":"14","author":"Manjula B. N.","year":"2003","journal-title":"Bioconjugate Chem."},{"key":"ref15\/cit15","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1007\/s10930-005-7837-2","volume":"24","author":"Manjula B. N.","year":"2005","journal-title":"Protein J."},{"key":"ref16\/cit16","unstructured":"Acharya, A. S., Manjula, B. N., and Smith. P.Hemoglobin crosslinkers, US patent 585,484,December17,1996."},{"key":"ref17\/cit17","unstructured":"Acharya, A. S., Manjula, B. N., and Smith.P.Hemoglobin crosslinkers, US patent 017,943,January25,2000."},{"key":"ref18\/cit18","first-page":"45","volume-title":"Biochemistry","author":"Stryer L.","year":"1995","edition":"4"},{"key":"ref19\/cit19","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/0003-2697(84)90307-5","volume":"136","author":"Heinrikson R. L.","year":"1984","journal-title":"Anal. Biochem."},{"key":"ref20\/cit20","doi-asserted-by":"crossref","first-page":"1325","DOI":"10.1016\/0006-291X(82)91258-X","volume":"106","author":"Currell D. L.","year":"1982","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref21\/cit21","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1111\/j.1432-1033.1986.tb10451.x","volume":"161","author":"Bellelli A.","year":"1986","journal-title":"Eur. J. Biochem."},{"key":"ref22\/cit22","doi-asserted-by":"crossref","first-page":"3329","DOI":"10.1073\/pnas.88.8.3329","volume":"88","author":"Manning L. R.","year":"1991","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"ref23\/cit23","doi-asserted-by":"crossref","first-page":"1804","DOI":"10.1021\/bi00405a062","volume":"27","author":"Kavanaugh M. P.","year":"1988","journal-title":"Biochemistry"},{"key":"ref24\/cit24","unstructured":"Hermanson, T., G.(1995)The Chemistry of Reactive Group. Bioconjugate Techniques, pp138\u2212168,Pierce Chemical Company,Rockford, IL."},{"key":"ref25\/cit25","unstructured":"Smith, P. K.Activated Polyethylene Glycol Compounds, US Patent 6,737,524,May,18,2004."},{"key":"ref26\/cit26","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1042\/CS20040001","volume":"107","author":"Assaly R. A.","year":"2004","journal-title":"Clin. Sci."},{"key":"ref27\/cit27","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1002\/hep.20720","volume":"41","author":"Quinlan G. J.","year":"2005","journal-title":"Hepatology"},{"key":"ref28\/cit28","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1097\/01.CCM.0000190623.97200.82","volume":"34","author":"Hangai-Hoger N.","year":"2006","journal-title":"Crit. Care Med."},{"key":"ref29\/cit29","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1097\/01.shk.0000167111.80753.ef","volume":"24","author":"Cabrales P.","year":"2005","journal-title":"Shock"},{"key":"ref30\/cit30","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/0003-2697(92)90585-U","volume":"205","author":"Zhang Y.","year":"1992","journal-title":"Anal. Biochem."},{"key":"ref31\/cit31","doi-asserted-by":"crossref","first-page":"5527","DOI":"10.1074\/jbc.275.8.5527","volume":"275","author":"Manjula B. N.","year":"2000","journal-title":"J. Biol. Chem."},{"key":"ref32\/cit32","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","volume":"72","author":"Bradford M.","year":"1976","journal-title":"Anal. Biochem."},{"key":"ref33\/cit33","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/0003-2697(87)90208-9","volume":"165","author":"Jackson C. J.","year":"1987","journal-title":"Anal. Biochem."},{"key":"ref34\/cit34","doi-asserted-by":"crossref","first-page":"3742","DOI":"10.1021\/ma00218a005","volume":"23","author":"Dust J. M.","year":"1990","journal-title":"Macromolecules"},{"key":"ref35\/cit35","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1039\/p29910000303","volume":"2","author":"Satchell D. P. N.","year":"1991","journal-title":"J. Chem. Soc., Perkin Trans."},{"key":"ref36\/cit36","doi-asserted-by":"crossref","first-page":"1304","DOI":"10.1021\/bc049843w","volume":"15","author":"Fee C. J.","year":"2004","journal-title":"Bioconjugate Chem."},{"key":"ref37\/cit37","doi-asserted-by":"crossref","first-page":"3269","DOI":"10.1016\/j.bmc.2004.03.073","volume":"12","author":"Trynda-Lemiesz L.","year":"2004","journal-title":"Bioorg. Med. Chem."},{"key":"ref38\/cit38","doi-asserted-by":"crossref","first-page":"2198","DOI":"10.1016\/S0006-3495(99)77375-3","volume":"76","author":"Chadborn N.","year":"1999","journal-title":"Biophys. J."},{"key":"ref39\/cit39","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/j.ab.2007.11.034","volume":"374","author":"Ananda K.","year":"2008","journal-title":"Anal. Biochem."},{"key":"ref40\/cit40","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1042\/BJ20050663","volume":"392","author":"Hu T.","year":"2005","journal-title":"Biochem. J."},{"key":"ref41\/cit41","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1007\/s10930-006-9010-y","volume":"25","author":"Li D.","year":"2006","journal-title":"Protein J."},{"key":"ref42\/cit42","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1042\/BJ20061434","volume":"402","author":"Hu T.","year":"2007","journal-title":"Biochem. J."}],"container-title":["Bioconjugate Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/bc7003878","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,3,5]],"date-time":"2023-03-05T22:33:11Z","timestamp":1678055591000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/doi\/10.1021\/bc7003878"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,6,24]]},"references-count":41,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2008,7,1]]}},"alternative-id":["10.1021\/bc7003878"],"URL":"https:\/\/doi.org\/10.1021\/bc7003878","relation":{},"ISSN":["1043-1802","1520-4812"],"issn-type":[{"value":"1043-1802","type":"print"},{"value":"1520-4812","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,6,24]]}}}