{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T12:25:57Z","timestamp":1790166357122,"version":"4.1.0"},"reference-count":15,"publisher":"American Chemical Society (ACS)","issue":"51","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biochemistry"],"published-print":{"date-parts":[[2001,12,1]]},"DOI":"10.1021\/bi011368r","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T04:45:26Z","timestamp":1027658726000},"page":"15811-15823","source":"Crossref","is-referenced-by-count":23,"title":["Kinetic and Mechanistic Studies of Penicillin-Binding Protein 2x from <i>Streptococcus pneumoniae<\/i>"],"prefix":"10.1021","volume":"40","author":[{"given":"Beth","family":"Thomas","sequence":"first","affiliation":[{"name":"Department of Chemical Enzymology, DuPont Pharmaceuticals Company, Wilmington, Delaware 19880"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Chemical Enzymology, DuPont Pharmaceuticals Company, Wilmington, Delaware 19880"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ross L.","family":"Stein","sequence":"additional","affiliation":[{"name":"Department of Chemical Enzymology, DuPont Pharmaceuticals Company, Wilmington, Delaware 19880"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2001,12,1]]},"reference":[{"key":"bi011368rb00001\/bi011368rb00001_1","first-page":"67","volume":"45","author":"Ghuysen J.-M.","year":"1991","journal-title":"Annu. Rev. Microbiol."},{"key":"bi011368rb00002\/bi011368rb00002_1","first-page":"60","volume":"8","author":"Ghuysen J.-M.","year":"1997","journal-title":"Internat. J. Antimicrob. Agents"},{"key":"bi011368rb00003\/bi011368rb00003_1","doi-asserted-by":"crossref","DOI":"10.1128\/MMBR.62.4.1079-1093.1998","volume":"62","author":"Goffin C.","year":"1998","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"bi011368rb00004\/bi011368rb00004_1","doi-asserted-by":"crossref","DOI":"10.1111\/j.1574-6968.1993.tb05954.x","volume":"106","author":"Kell C.","year":"1993","journal-title":"FEMS Microbiol. Lett."},{"key":"bi011368rb00005\/bi011368rb00005_1","volume-title":"Nat. Struct. Biol. 3, 284\u2212289","author":"Pares S.","year":"1996"},{"key":"bi011368rb00006\/bi011368rb00006_1","doi-asserted-by":"crossref","DOI":"10.1006\/jmbi.2000.3740","volume":"299","author":"Gordon E.","year":"2000","journal-title":"J. Mol. Biol."},{"key":"bi011368rb00007\/bi011368rb00007_1","doi-asserted-by":"crossref","DOI":"10.1042\/bj2920735","volume":"292","author":"Jamin M.","year":"1993","journal-title":"Biochem. J."},{"key":"bi011368rb00008\/bi011368rb00008_1","doi-asserted-by":"crossref","DOI":"10.1111\/j.1432-1033.1992.tb17128.x","volume":"207","author":"Laible G.","year":"1992","journal-title":"Eur. J. Biochem."},{"key":"bi011368rb00009\/bi011368rb00009_1","volume":"62","author":"Brown R. S.","year":"1997","journal-title":"J. Org. Chem."},{"key":"bi011368rb00010\/bi011368rb00010_1","volume-title":"in Enzyme Mechanism from Isotope Effects","author":"Quinn D. M.","year":"1991"},{"key":"bi011368rb00011\/bi011368rb00011_1","unstructured":"Jencks, W. P. (1969).Catalysis in Chemistry and Enzymology, pp 517\u2212523, McGraw-Hill, New York."},{"key":"bi011368rb00012\/bi011368rb00012_1","unstructured":"Fersht, A. (1977)Enzyme Structure and Mechanism, pp 172\u2212174, W. H. Freeman and Company, New York."},{"key":"bi011368rb00013\/bi011368rb00013_1","volume":"107","author":"Stein R. L.","year":"1985","journal-title":"J. Am. Chem. Soc."},{"key":"bi011368rb00014\/bi011368rb00014_1","volume-title":"Biochemistry 26, 1305\u22121314","author":"Stein R. L.","year":"1987"},{"key":"bi011368rn00001\/bi011368rn00001_1","volume-title":"penicillin-binding protein","author":"Abbreviations PBP"}],"container-title":["Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/bi011368r","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,3,6]],"date-time":"2023-03-06T09:17:43Z","timestamp":1678094263000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/doi\/10.1021\/bi011368r"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,12,1]]},"references-count":15,"journal-issue":{"issue":"51","published-print":{"date-parts":[[2001,12,1]]}},"alternative-id":["10.1021\/bi011368r"],"URL":"https:\/\/doi.org\/10.1021\/bi011368r","relation":{},"ISSN":["0006-2960","1520-4995"],"issn-type":[{"value":"0006-2960","type":"print"},{"value":"1520-4995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,12,1]]}}}