{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T21:04:50Z","timestamp":1787000690333,"version":"build-2736575974"},"reference-count":43,"publisher":"American Chemical Society (ACS)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biochemistry"],"published-print":{"date-parts":[[2003,1,1]]},"DOI":"10.1021\/bi0267339","type":"journal-article","created":{"date-parts":[[2003,1,14]],"date-time":"2003-01-14T01:14:23Z","timestamp":1042506863000},"page":"497-508","source":"Crossref","is-referenced-by-count":20,"title":["Allosteric Binding Properties of a Monoclonal Antibody and Its Fab Fragment"],"prefix":"10.1021","volume":"42","author":[{"given":"Robert C.","family":"Blake","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Tulane University Health Sciences Center, 1430 Tulane Avenue, New Orleans, Louisiana 70112, College of Pharmacy, Xavier University of Louisiana, New Orleans, Louisiana 70125, and Tulane\/Xavier Center for Bioenvironmental Research, New Orleans, Louisiana 70112"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James B.","family":"Delehanty","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Tulane University Health Sciences Center, 1430 Tulane Avenue, New Orleans, Louisiana 70112, College of Pharmacy, Xavier University of Louisiana, New Orleans, Louisiana 70125, and Tulane\/Xavier Center for Bioenvironmental Research, New Orleans, Louisiana 70112"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehraban","family":"Khosraviani","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Tulane University Health Sciences Center, 1430 Tulane Avenue, New Orleans, Louisiana 70112, College of Pharmacy, Xavier University of Louisiana, New Orleans, Louisiana 70125, and Tulane\/Xavier Center for Bioenvironmental Research, New Orleans, Louisiana 70112"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haini","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Tulane University Health Sciences Center, 1430 Tulane Avenue, New Orleans, Louisiana 70112, College of Pharmacy, Xavier University of Louisiana, New Orleans, Louisiana 70125, and Tulane\/Xavier Center for Bioenvironmental Research, New Orleans, Louisiana 70112"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. Mark","family":"Jones","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Tulane University Health Sciences Center, 1430 Tulane Avenue, New Orleans, Louisiana 70112, College of Pharmacy, Xavier University of Louisiana, New Orleans, Louisiana 70125, and Tulane\/Xavier Center for Bioenvironmental Research, New Orleans, Louisiana 70112"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diane A.","family":"Blake","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Tulane University Health Sciences Center, 1430 Tulane Avenue, New Orleans, Louisiana 70112, College of Pharmacy, Xavier University of Louisiana, New Orleans, Louisiana 70125, and Tulane\/Xavier Center for Bioenvironmental Research, New Orleans, Louisiana 70112"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2002,12,19]]},"reference":[{"key":"bi0267339b00001\/bi0267339b00001_1","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/0014-5793(74)80883-5","volume":"40","author":"Carayon P.","year":"1974","journal-title":"FEBS Lett."},{"key":"bi0267339b00002\/bi0267339b00002_1","volume-title":"Monoclonal antibodies to human growth hormone induce an allosteric conformational change in the antigen. Immunology 67, 148\u2212153","author":"Mazza M. M.","year":"1989"},{"key":"bi0267339b00003\/bi0267339b00003_1","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1007\/BF00931602","volume":"136","author":"Aguilar R. C.","year":"1994","journal-title":"Mol. Cell Biochem."},{"key":"bi0267339b00004\/bi0267339b00004_1","doi-asserted-by":"crossref","DOI":"10.1002\/(SICI)1099-1352(199907\/08)12:4<242::AID-JMR461>3.0.CO;2-1","volume":"12","author":"Reineke U.","year":"1999","journal-title":"J. Mol. Recognit."},{"key":"bi0267339b00005\/bi0267339b00005_1","volume-title":"Thermodynamics and kinetics of the reaction of a single-chain antibody fragment (scFv) with the leucine zipper domain of transcription factor GCN4. Biochemistry 37, 13011\u221213020","author":"Weber-Bornhauser S.","year":"1998"},{"key":"bi0267339b00006\/bi0267339b00006_1","volume-title":"Proc. Natl. Acad. Sci. U.S.A. 99","author":"Nowakowski A.","year":"2002"},{"key":"bi0267339b00007\/bi0267339b00007_1","doi-asserted-by":"crossref","DOI":"10.1016\/0022-1759(91)90376-Q","volume":"140","author":"van Erp R.","year":"1991","journal-title":"J. Immunol. Met."},{"key":"bi0267339b00008\/bi0267339b00008_1","volume":"258","author":"Holmes N. J.","year":"1983","journal-title":"J. Biol. Chem."},{"key":"bi0267339b00009\/bi0267339b00009_1","volume-title":"Enhanced binding activity observed between anti-carcinoembryonic monoclonal antibodies. Hybridoma 5, 199\u2212213","author":"Wellerson R.","year":"1986"},{"key":"bi0267339b00010\/bi0267339b00010_1","volume-title":"Cooperative immunoassays:\u2009 ultrasensitive assays with mixed monoclonal antibodies. Science 221, 279\u2212281","author":"Ehrlich P. H.","year":"1983"},{"key":"bi0267339b00011\/bi0267339b00011_1","doi-asserted-by":"crossref","DOI":"10.4049\/jimmunol.131.4.1900","volume":"131","author":"Moyle W. R.","year":"1983","journal-title":"J. Immunol."},{"key":"bi0267339b00012\/bi0267339b00012_1","volume-title":"Specificity considerations in cooperative immunoassays. Clin. Chem. 30, 1523\u22121532","author":"Ehrlich P. H.","year":"1984"},{"key":"bi0267339b00013\/bi0267339b00013_1","volume-title":"Quantitative explanation for increased affinity shown by mixtures of monoclonal antibodies:\u2009 importance of a circular complex. Mol. Immunol. 20, 439\u2212452","author":"Moyle W. R.","year":"1983"},{"key":"bi0267339b00014\/bi0267339b00014_1","volume":"271","author":"Blake D. A.","year":"1996","journal-title":"J. Biol. Chem."},{"key":"bi0267339b00015\/bi0267339b00015_1","volume-title":"Binding properties of a monoclonal antibody directed toward lead-chelate complexes. Bioconj. Chem. 11, 267\u221277","author":"Khosraviani M.","year":"2000"},{"key":"bi0267339b00016\/bi0267339b00016_1","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/S0003-2670(01)01151-5","volume":"444","author":"Blake D. A.","year":"2001","journal-title":"Anal. Chim. Acta"},{"key":"bi0267339b00017\/bi0267339b00017_1","volume-title":"Monoclonal antibodies that recognize minimal differences in the three- dimensional structures of metal-chelate complexes. Bioconj. Chem. 13, 408\u2212415","author":"Jones R. M.","year":"2002"},{"key":"bi0267339b00018\/bi0267339b00018_1","volume":"2","author":"Brechbiel M.","year":"1986","journal-title":"J. Inorg. Chem."},{"key":"bi0267339b00019\/bi0267339b00019_1","doi-asserted-by":"crossref","DOI":"10.1006\/abio.1997.2161","volume-title":"The quantification of protein amino groups by the trinitrobenzenesulfonic acid method:\u2009 a reexamination. Anal. Biochem. 249, 184\u2212200","author":"Cayot P.","year":"1997"},{"key":"bi0267339b00020\/bi0267339b00020_1","first-page":"5","volume":"21","author":"Chakrabarti P.","year":"1994","journal-title":"Anal. Biochem."},{"key":"bi0267339b00021\/bi0267339b00021_1","volume-title":"Immunoaffinity chromatography of proteins. Met. Enzymol. XXXIV, 723\u2212731","author":"Livingston D. M.","year":"1974"},{"key":"bi0267339b00022\/bi0267339b00022_1","volume-title":"NIST Standard Reference Data, National Institutes of Standards and Technology.","author":"Martell A. E.","year":"1998"},{"key":"bi0267339b00023\/bi0267339b00023_1","doi-asserted-by":"crossref","DOI":"10.1021\/jm980152t","volume":"41","author":"McMurry T. J.","year":"1998","journal-title":"J. Med. Chem."},{"key":"bi0267339b00024\/bi0267339b00024_1","unstructured":"Kennedy, J. H. (1984) in\n                      Analytical Chemistry:\u2009 Principles\n                      , pp 281\u2212303, Harcourt Brace Jovanovich, New York."},{"key":"bi0267339b00025\/bi0267339b00025_1","volume-title":"Automated kinetic exclusion assays to quantify protein binding interactions in homogeneous solution. Anal. Biochem. 272, 123\u221234","author":"Blake R. C., II","year":"1999"},{"key":"bi0267339b00026\/bi0267339b00026_1","volume-title":"II. (1997) in Immunochemical Technology for Environmental Applications","author":"Blake D. A."},{"key":"bi0267339b00027\/bi0267339b00027_1","first-page":"83","volume":"29","author":"Weber G.","year":"1975","journal-title":"Adv. Protein. Chem."},{"key":"bi0267339b00028\/bi0267339b00028_1","volume-title":"Biochemistry 11, 864\u2212878","author":"Weber G.","year":"1972"},{"key":"bi0267339b00029\/bi0267339b00029_1","volume-title":"Kinetics of enzyme reactions with interaction between a substrate and a (metal) modifier. Biochemistry 8, 1767\u22121779","author":"London W. P.","year":"1969"},{"key":"bi0267339b00030\/bi0267339b00030_1","volume-title":"Approaches to kinetic studies on metal-activated enzymes. Met. Enzymol. 63, 257\u2212294","author":"Morrison J. F.","year":"1979"},{"key":"bi0267339b00031\/bi0267339b00031_1","doi-asserted-by":"crossref","DOI":"10.1006\/jmbi.1994.1133","volume":"236","author":"Guddat L. W.","year":"1994","journal-title":"J. Mol. Biol."},{"key":"bi0267339b00032\/bi0267339b00032_1","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/0969-2126(93)90024-B","volume":"1","author":"Stanfield R. L.","year":"1993","journal-title":"Structure"},{"key":"bi0267339b00033\/bi0267339b00033_1","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1006\/bbrc.1998.9380","volume":"251","author":"Kodandapani R.","year":"1998","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"bi0267339b00034\/bi0267339b00034_1","doi-asserted-by":"crossref","unstructured":"Monaco-Malbet, S., Berthet-Colominas, C., Novelli, A., Battai, N., Piga, N., Cheynet, V., Mallet, F., and Cusack, S. (2000) Mutual conformational adaptations in antigen and antibody upon complex formation between an Fab and HIV-1 capsid protein p24.\n                      Struct. Fold Des. 8\n                      , 1069\u22121077.","DOI":"10.1016\/S0969-2126(00)00507-4"},{"key":"bi0267339b00035\/bi0267339b00035_1","volume-title":"Effects of secondary forces on the ligand binding properties and variable domain conformations of a monoclonal anti-fluorescyl antibody. Mol. Immunol. 33, 1067\u22121077","author":"Mummert M. E.","year":"1996"},{"key":"bi0267339b00036\/bi0267339b00036_1","volume-title":"Effects of secondary forces on the ligand binding and conformation state of antifluorescein monoclonal antibody 9\u221240. Biochemistry 36, 11918\u221211922","author":"Mummert M. E.","year":"1997"},{"key":"bi0267339b00037\/bi0267339b00037_1","volume-title":"Effects of secondary forces on a high affinity monoclonal IgM anti-fluorescein antibody possessing cryoglobulin and other cross-reactive properties. Mol. Immunol. 35, 103\u2212113","author":"Mummert M. E.","year":"1996"},{"key":"bi0267339b00038\/bi0267339b00038_1","volume":"17","author":"Mummert M. E.","year":"1996","journal-title":"J. Protein Chem."},{"key":"bi0267339b00039\/bi0267339b00039_1","volume-title":"Long-range changes in a protein antigen due to antigen\u2212antibody interaction. Biochemistry 31, 9539\u22129545","author":"Benjamin D. C.","year":"1992"},{"key":"bi0267339b00040\/bi0267339b00040_1","doi-asserted-by":"crossref","DOI":"10.4049\/jimmunol.148.2.503","volume":"148","author":"Lavoie T. B.","year":"1992","journal-title":"J. Immunol."},{"key":"bi0267339b00041\/bi0267339b00041_1","volume-title":"Proc. Natl. Acad. Sci. U.S.A. 86","author":"Padlan E. A.","year":"1989"},{"key":"bi0267339b00042\/bi0267339b00042_1","volume-title":"Cooperativity of binding of anilinonaphthalenesulfonate to serum albumin induced by a second ligand. Biochem. 14, 4476\u22124481","author":"Kolb D. A.","year":"1975"},{"key":"bi0267339n00001\/bi0267339n00001_1","volume-title":"trans-cyclohexyldiethylenetriamine-N,N\u2018,N\u2018,N\u2018\u2009\u2018,N\u2018\u2009\u2018-pentaacetic acid","author":"Abbreviations CHX-DTPA"}],"container-title":["Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/bi0267339","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,8]],"date-time":"2024-01-08T14:38:58Z","timestamp":1704724738000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/doi\/10.1021\/bi0267339"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,12,19]]},"references-count":43,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2003,1,1]]}},"alternative-id":["10.1021\/bi0267339"],"URL":"https:\/\/doi.org\/10.1021\/bi0267339","relation":{},"ISSN":["0006-2960","1520-4995"],"issn-type":[{"value":"0006-2960","type":"print"},{"value":"1520-4995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,12,19]]}}}